DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(381) Inhibition of the Tcf/beta-catenin complex increases apoptosis and impairs adrenocortical tumor cell proliferation and adrenal steroidogenesisOncotargetDecember 15, 2015Leal LF, Bueno AC, Gomes DC, Abduch R, de Castro M, Antonini SR.counterstaining, cells were incubated with 4′,6-diamidino-2-phenylidone (DAPi; Cell Signaling Technology, #4083, dilution: 1:25,000) for 2 minutes, washed in 1X PBS, and slides were set with Fluoromount (Sigma-Aldrich).

(382) KPT-330 has antitumour activity against non-small cell lung cancerBritish journal of cancerJuly 15, 2014Sun H, Hattori N, Chien W, Sun Q, Sudo M, E-Ling GL, Ding L, Lim SL, Shacham S, Kauffman M, et al.Louis, MO, USA). Wortmannin (product number 9951) and 4′, 6-diamidino-2-phenylindole (product number 4083) were purchased from Cell Signaling Technology (Danvers, MA, USA). Flag-hCRM1 plasmid was purchased

(383) Neuroprotection by sodium ferulate against glutamate-induced apoptosis is mediated by ERK and PI3 kinase pathwaysActa Pharmacologica SinicaDecember 1, 2007Ying JIN, En-zhi YAN, Ying FAN, Xiao-li GUO, Yan-jie ZHAO, Zhi-hong ZONG, Zhuo LIUprotein S6 protein kinase (p70S6K; Thr389), and Bcl-2 antibodies 1882 Acta Pharmacologica Sinica ISSN 1671-4083 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The β-actin antibody and Hoechst 33258

(384) Cytotoxicity, apoptosis induction, and mitotic arrest by a novel podophyllotoxin glucoside, 4DPG, in tumor cells1Acta Pharmacologica SinicaAugust 1, 2005Yi-lin QI, Fan LIAO, Chang-qi ZHAO, Yong-da LIN, Ming-xue ZUOcells were fixed and incubated with cleaved caspase-3 (Asp175) 1002 Acta Pharmacologica Sinica ISSN 1671-4083 antibody (Cell Signaling, Beverly, USA) as a primary antibody, which specifically recognizes activated

(385) Interleukin-1 beta induction of neuron apoptosis depends on p38 mitogen-activated protein kinase activity after spinal cord injury1Acta Pharmacologica SinicaAugust 1, 2005Xin-jia WANG, Kang-mei KONG, Wei-li QI, Wei-lian YE, Pei-song SONGafter injury (n=3 per time point), an 8-mm spinal cord segment was 936 Acta Pharmacologica Sinica ISSN 1671-4083 dissected 4 mm rostral and 4 mm caudal from the lesion epicenter from each group. The cord tissues

(386) Inhibiting Caspase-12 Mediated Inflammasome Activation protects against Oxygen-Glucose Deprivation Injury in Primary AstrocytesInternational journal of medical sciencesJanuary 1, 0001"Liu L, Chen M, Lin K, Xiang X, Zheng Y, Zhu S."SA00013-4) were added for 1 h. After three washes with PBS, 1 μg/ml DAPI (Cell Signaling Technology, #4083) solution was added to cover the cells for 5 min in the dark followed by three washes with PBS. Fluorescence

(387) FoxA2 and RNA Pol II mediate human islet amyloid polypeptide turnover in ER-stressed pancreatic β-cellsThe Biochemical journalJanuary 1, 0001"Chatterjee Bhowmick D, Burnett L, Kudaibergenova Z, Jeremic AM."minutes at RT. Nuclei were stained with nuclear-specific dye, DAPI (Cell Signaling Technology, cat# 4083) for 10 min at RT. The following antibodies (Abcam) were used: ER marker, anti-calnexin (1 : 500, cat#

(388) Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinomaCell death & diseaseJanuary 1, 0001"Ling Z, Li W, Hu J, Li Y, Deng M, Zhang S, Ren X, Wu T, Xia J, Cheng B, et al."incubation in the dark at room temperature. The cells were then counterstained with 0.5 ug/mL of DAPI (#4083 s, CST, Danvers, MA, USA) for 5 min at room temperature and washed with PBS three times. The samples

(389) A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleusNature communicationsJanuary 1, 0001"Simion V, Zhou H, Haemmig S, Pierce JB, Mendes S, Tesmenitsky Y, Pérez-Cremades D, Lee JF, Chen AF, Ronda N, et al."temperature. Nuclei were stained for 5 min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5 µg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen, P36930). Images

(390) Targeting the succinate receptor effectively inhibits periodontitisCell reportsJanuary 1, 0001"Guo Y, Xu F, Thomas SC, Zhang Y, Paul B, Sakilam S, Chae S, Li P, Almeter C, Kamer AR, et al."/14190-136 Succinate,Sigma,14160-100G EDTA,Fisher Chemical,E478-1 BlockAid™ Blocking Solution,Invitrogen,B10710 DAPI,Cell Signaling Technology,4083 ActinGreen™ 488,Invitrogen,R37110 Sodium acetate,Sigma,S2889 L-(+) Tartaric acid,Sigma,T6521 Glacial

(391) Dysfunction of estrogen-related receptor alpha-dependent hepatic VLDL secretion contributes to sex disparity in NAFLD/NASH developmentTheranosticsJanuary 1, 0001"Yang M, Liu Q, Huang T, Tan W, Qu L, Chen T, Pan H, Chen L, Liu J, Wong CW, et al."(1μg/mL, Sigma, N3013) for 10 min at room temperature. Nuclei were stained using DAPI (Cell signaling, 4083) and mounted with prolong gold anti-fade reagent (Invitrogen, P10144) followed by washing in PBS for

(392) Enhancer hijacking activates GFI1 family oncogenes in medulloblastomaNatureJanuary 1, 0001"Northcott PA, Lee C, Zichner T, Stütz AM, Erkek S, Kawauchi D, Shih DJ, Hovestadt V, Zapatka M, Sturm D, et al."Anti-Rabbit IgG, Invitrogen A10042). Sections were then counter-stained with DAPI (Cell Signaling #4083), mounted using Fluoromount G (Southern Biotech #0100-01), and imaged on a confocal (Zeiss LSM700) fluorescent microscope

(393) Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microgliaJournal of neuroinflammationJanuary 1, 0001"Xiang X, Tang X, Yu Y, Xie S, Liu L, Chen M, Zhang R, Kang X, Zheng Y, Yang G, et al."1:400) for 1 h at room temperature. Sections were then washed in PBS, incubated with DAPI (1:1000, CST, #4083) in PBS for 10 min. Subsequently, sections were washed in PBS buffer, mounted onto microscope slides

(394) A novel epigenetic AML1-ETO/THAP10/miR-383 mini-circuitry contributes to t(8;21) leukaemogenesisEMBO molecular medicineJanuary 1, 0001"Li Y, Ning Q, Shi J, Chen Y, Jiang M, Gao L, Huang W, Jing Y, Huang S, Liu A, et al."USA) diluted in PBA‐BSA. After three PBS washes, nuclei were counterstained with DAPI (0.2 μg/ml, #4083, Cell Signalling Technology, USA). Following extensive washing in PBS, samples were air‐dried and mounted

(395) Effect of Hypoxia-Ischemia on the Expression of Iron-Related Proteins in Neonatal Rat BrainsNeural plasticityJanuary 1, 0001"Lin Q, Hu DW, Hao XH, Zhang G, Lin L."ab150117) for 1 hour at room temperature. Sections were subsequently washed in PBST and DAPI (CST, 4083). The results were visualized and photographed under a confocal laser-scanning microscope (Leica, TCS

(396) Brainstem Nuclei Associated with Mediating Apnea-Induced Respiratory Motor PlasticityScientific reportsJanuary 1, 0001"Lui S, Torontali Z, Tadjalli A, Peever J."RRID: AB_2313593). They were then counterstained with DAPI (1:1000 dilution, Cell Signalling, Cat# 4083 S, lot# 0007). Sections with viral vector were incubated in primary rabbit anti-mCherry (1:1000, Novus Biologicals

(397) Pathological significance and therapeutic prospects of HCCS expression patterns in lung adenocarcinomaCell adhesion & migrationJanuary 1, 0001"Luo P, Wu S, Li Z, Tan S, Zeng L, Tang Y, Luo L, Li Y, Tang Z."fluorescent marker for simultaneous detection of both markers. Nuclei were stained with DAPI (Catalog No. 4083, CST) for 8 minutes. Finally, fluorescently labeled cells were imaged using a fluorescence microscope,

(398) A spatiotemporal steroidogenic regulatory network in human fetal adrenal glands and gonadsFrontiers in endocrinologyJanuary 1, 0001"Wang Y, Guo B, Guo Y, Qi N, Lv Y, Ye Y, Huang Y, Long X, Chen H, Su C, et al."The slides were washed three times in PBS for 5 min each, and the nuclei were then stained with DAPI (4083S, CST). Fluorescence images were captured using a laser scanning confocal microscope (TCS SP8, Laika

(399) Sex and diet-dependent gene alterations in human and rat brains with a history of nicotine exposureFrontiers in psychiatryJanuary 1, 0001"Vargas-Medrano J, Carcoba LM, Vidal Martinez G, Mulla ZD, Diaz V, Ruiz-Velasco A, Alvarez-Primo F, Colina G, Iñiguez SD, Thompson PM, et al."4°C. Finally, the slides were washed twice with wash buffer. Cell nuclei were stained with DAPI (Cat# 4083S, Cell Signaling Technology Inc., Danvers, MA, USA), and mounted with Fluromount-G slide mounting medium

(400) PPAR agonists attenuate lenalidomide's anti-myeloma activity in vitro and in vivoCancer lettersJanuary 1, 0001"Sha Y, Wu J, Paul B, Zhao Y, Mathews P, Li Z, Norris J, Wang E, McDonnell DP, Kang Y."thrice with PBS, the slides were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 4083; Cell Signaling) for 5 min and mounted with the antifade mounting medium (Vector, H-1000). Images were

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DAPI

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