CviQI from New England Biolabs

Product Specs
ItemCviQI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0639S
Concentration10,000 units/ml
Quantity2000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GTAC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Optimized digital polymerase chain reaction enables detection of telomerase reverse transcriptase C228T mutation for prognostic assessment in hepatocellular carcinomaWJGODecember 15, 2025Zulihumaer Aizimuaji, Nan Hu, Hai-Yang Li, Xi-Jun Wang, Sheng Ma, Ya-Ru Wang, Rui-Qi Zheng, Zhuo Li, Huan Zhao, Wei-Qi Rong, et al.(dHsaEXD72405942), Bio-Rad, United States], restriction enzyme 0.2 μL (New England BioLabs; CviQI; R0639S, United States), 0.2 μL 7-Deaza-dGTP (New England BioLabs; N0445S, United States), 1 μL of 25 mmol/L

(2) Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic lociCell GenomicsNovember 12, 2025Ke Zhao, Yao Zhou, Xueqi Wang, Zhao Wang, Xinran Xu, Yichen Chen, Lin Zhao, Chengyue Wu, Jianhua Wang, Hongcheng Yao, et al.,New England Biolabs,Cat#R0543L T4 DNA Ligase,New England Biolabs,Cat#M0202L CviQI,New England Biolabs,Cat#R0639L NlaIII,New England Biolabs,Cat# R0125S EcoRI,New England Biolabs,Cat# R0101S T4 Polynucleotide Kinase

(3) Epigenetic editing at individual age-associated CpGs affects the genome-wide epigenetic aging landscapeNature AgingMarch 24, 2025Sven Liesenfelder, Mohamed H. Elsafi Mabrouk, Jessica Iliescu, Monica Varona Baranda, Athanasia Mizi, Juan-Felipe Perez-Correa, Martina Wessiepe, Argyris Papantonis, Wolfgang Wagnerdigested chromatin, nuclei were lysed, and the isolated DNA was digested with CviQI (New England Biolabs, R0639), circularized by ligation and purified. The library was amplified by inverse PCR with viewpoints-specific

(4) Integration of CTCF loops, methylome, and transcriptome in differentiating LUHMES as a model for imprinting dynamics of the 15q11-q13 locus in human neuronsHuman Molecular GeneticsSeptember 19, 2024Orangel J Gutierrez Fugón, Osman Sharifi, Nicholas Heath, Daniela C Soto, J Antonio Gomez, Dag H Yasui, Aron Judd P Mendiola, Henriette O’Geen, Ulrika Beitnere, Marketa Tomkova, et al.enzyme digest was performed using DpnII (Cat. No. R0543S) and secondary digestion with CviQI (Cat. No. R0639S) from NEB. Before sequencing a final cleanup using SPRI select beads from Beckman Coulter (Cat No. B23317)

(5) GAGE-seq concurrently profiles multiscale 3D genome organization and gene expression in single cellsNature GeneticsAugust 1, 2024Tianming Zhou, Ruochi Zhang, Deyong Jia, Raymond T. Doty, Adam D. Munday, Daniel Gao, Li Xin, Janis L. Abkowitz, Zhijun Duan, Jian Maenzyme (RE) digestion mix (1x T4 ligase buffer (NEB, B0202S), 500U MseI (NEB, R0525M), 240U CviQI (NEB, R0639L), 0.32 U/ml Enzymatics RNase Inhibitor, 0.05 U/ml SUPERase RNase Inhibitor), and incubated at room temperature

(6) Regulation of T helper cell differentiation by the interplay between histone modification and chromatin interactionImmunityMay 14, 2024Shuai Liu, Yaqiang Cao, Kairong Cui, Gang Ren, Tingting Zhao, Xuezheng Wang, Danping Wei, Zuojia Chen, Rama Krishna Gurram, Chengyu Liu, et al.K,Roche,Cat# 03115828001 T4 DNA polymerase,New England Biolabs,Cat# M0203L CviQI,New England Biolabs,Cat# R0639L CviAII,New England Biolabs,Cat# R0640L BfaI,New England Biolabs,Cat# R0568L MluCI,New England Biolabs,Cat#

(7) The transcription factor NF-κB orchestrates nucleosome remodeling during the primary response to Toll-like receptor 4 signalingImmunityMarch 1, 2024An-Chieh Feng, Brandon J. Thomas, Prabhat K. Purbey, Filipe Menegatti de Melo, Xin Liu, Allison E. Daly, Fei Sun, Jerry Hung-Hao Lo, Lijing Cheng, Michael F. Carey, et al.pmol) Choice-Taq™ DNA Polymerase,Deville,Cat#CB4050-2 CviQI restriction endonuclease,New England Biolabs,Cat#R0639L Cycloheximide (CHX),Sigma-Aldrich,Cat#239765 Digitonin,Promega,Cat#G9441 Dimethyl sulfoxide,Sigma-Aldrich,Cat#2438 "DNA Polymerase I, large (Klenow) fragment

(8) Concurrent profiling of multiscale 3D genome organization and gene expression in single mammalian cellsbioRxivJuly 27, 2023Tianming Zhou, Ruochi Zhang, Deyong Jia, Raymond T. Doty, Adam D. Munday, Daniel Gao, Li Xin, Janis L. Abkowitz, Zhijun Duan, Jian Maenzyme (RE) digestion mix (1x T4 ligase buffer (NEB, B0202S), 500U MseI (NEB, R0525M), 240U CviQI (NEB, R0639L), 0.32 U/ml Enzymatics RNase Inhibitor, 0.05 U/ml SUPERase RNase Inhibitor), and incubated at room temperature

(9) An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin loopingNature communicationsMarch 3, 2023Wang Z, Liang Q, Qian X, Hu B, Zheng Z, Wang J, Hu Y, Bao Z, Zhao K, Zhou Y, et al.R0125L) for the rs4728142-containing region (GRCh37/hg19 chr7: 128,573,714–128,574,338) or CviQI (NEB, R0639L) for the enhancer (GRCh37/hg19 chr7: 128,573,036–128,573,717) adjacent to the downstream rs4728142-containing

(10) RyR2/IRBIT regulates insulin gene transcript, insulin content, and secretion in the insulinoma cell line INS-1Scientific reportsMay 11, 2022Harvey KE, LaVigne EK, Dar MS, Salyer AE, Pratt EPS, Sample PA, Aryal UK, Gowher H, Hockerman GH.purified again. 14 μg of sample DNA were digested overnight at 25 °C with CviQI restriction enzyme (NEB, R0639L), which cuts outside the region of interest. The next day, the samples were purified, and 5 μg were

(11) Simplified MethylRAD Sequencing to Detect Changes in DNA Methylation at Enhancer Elements in Differentiating Embryonic Stem CellsEpigenomesApril 8, 2021Debapriya Saha, Allison B. Norvil, Nadia A. Lanman, Humaira Gowherisolation, followed by ethanol precipitation. DNA from samples was digested first with CviQI (NEB, R0639L) overnight at 25°C. Purified samples were then digested with FspEI (NEB) overnight at 37°C. The digested

(12) Quantitative Assessment of Parental Somatic Mosaicism for Copy‐Number Variant (CNV) DeletionsCurrent Protocols in Human GeneticsJune 1, 2020Qian Liu, Christopher M. Grochowski, Weimin Bi, James R. Lupski, Paweł Stankiewiczno. R0525S), AluI (NEB, cat. no. R0137S), HindIII (NEB, cat. no. R3104S), and CviQI (NEB, cat. no. R0639S) are recommended by Bio-Rad. Eppendorf™ 96-Well twin.tec™ PCR Plate, semi-skirt (Fisher Scientific,

(13) A non-coding genetic variant associated with abdominal aortic aneurysm alters ERG gene regulationHuman Molecular GeneticsMarch 13, 2020Judith Marsman, Gregory Gimenez, Robert C Day, Julia A Horsfield, Gregory T Jonesphenol/chloroform extraction and ethanol precipitated. A second digestion was performed with 25 units CviQI (R0639L, NEB) by incubating at 25◦ C overnight at 30 rpm. The restriction enzyme was removed via phenol/chloroform extraction

(14) A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cellsNature communicationsFebruary 6, 2020Guo L, Li J, Zeng H, Guzman AG, Li T, Lee M, Zhou Y, Goodell MA, Stephan C, Davies PJA, et al.phenol-chloroform extraction method followed by ethanol precipitation. Purified DNA was then digested by BfaI (NEB R0639L, 50 units) overnight followed by a second ligation using T4 DNA ligase (NEB 0202 M, 6000 units) at 16

(15) High-resolution mapping of a standardized transcriptional reporter reveals positions of high and low transcription in the Escherichia coli chromosomeCell SystemsMarch 1, 2019Scott A. Scholz, Rucheng Diao, Michael B. Wolfe, Elayne M. Fivenson, Xiaoxia Nina Lin, Peter L. Freddolinoof the resulting DNA was digested separately with each of CviAII (NEB Cat#0640) and CviQI (NEB Cat#R0639) restriction enzymes (each has a different 4 bp cut site but leaves compatible overhangs; the use of

(16) Promoter-Enhancer Communication Occurs Primarily within Insulated NeighborhoodsMolecular cellJanuary 17, 2019Sun F, Chronis C, Kronenberg M, Chen XF, Su T, Lay FD, Plath K, Kurdistani SK, Carey MF.,Thermo Fisher Scientific,Cat#11205D DpnII,New England Biolabs,Cat#R0543M NlaIII,New England Biolabs,Cat#R0125L CviQI,New England Biolabs,Cat#R0639L T4 DNA Ligase,New England Biolabs,Cat#M0202M 3X FLAG Peptide,Sigma-Aldrich,Cat#F4799 Anti-FLAG M2

(17) YY1 Is a Structural Regulator of Enhancer-Promoter LoopsCellDecember 14, 2017Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, Abraham BJ, Cohen MA, Nabet B, Buckley DL, et al.overnight in 450 μL with 50 units of restriction enzyme. BfaI (NEB R0568S) was used for Etv4 and CviQI (NEB R0639S) was used for Raf1. Samples were phenol-chloroform extracted and ethanol precipitated and the second

(18) A refined DNA methylation detection method using MspJI coupled quantitative PCRAnalytical BiochemistrySeptember 1, 2017Christopher J. Petell, Gilbert Loiseau, Ryan Gandy, Sriharsa Pradhan, Humaira Gowherphenol:chloroform isolation was digested (10 μg) overnight at 25 °C with 40 U of CviQI restriction enzyme (NEB, R0639L), which cuts outside the region of interest. After phenol:chloroform extraction, these samples were

(19) The non-coding variant rs1800734 enhances DCLK3 expression through long-range interaction and promotes colorectal cancer progressionNature CommunicationsApril 1, 2017Ning Qing Liu et al.ligated DNA was de-crosslinked, puriﬁed, digested with 90 U CviQI enzyme (New England BioLabs Inc., R0639S) and circularized by 5,000 U T4 ligase. The circularized DNA (16 300 ng) was ampliﬁed with bait-speciﬁc

(20) An epigenetic switch regulatesde novoDNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiationNucleic Acids ResearchSeptember 19, 2016Christopher J. Petell, Lama Alabdi, Ming He, Phillip San Miguel, Richard Rose, Humaira Gowherfollowed by ethanol precipitation. DNA from various samples was digested initially with CviQI (NEB, R0639L) overnight at 25°C. Purified samples were subjected to digestion by MspJI (NEB, R0166L) overnight at

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CviQI

CviQI from New England Biolabs

Description

Sequence

New England Biolabs

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Reviews

CviQI from New England Biolabs

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