TNT T7/SP6 Coupled Wheat Germ Extract System from Promega

Product Specs
ItemTNT T7/SP6 Coupled Wheat Germ Extract System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL5030
Quantity40 reactions
SystemT7/SP6 Coupled Wheat Germ Extract System

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protocol for chloroplast purification and import assay in ArabidopsisSTAR ProtocDecember 6, 2025Huan Zhao, Xinyue Wang, Liangsheng Wangwheat germ extract system, according to the manufacturer’s instructions (www.promega.com.cn, Promega, L5030). Use the SP6 RNA polymerase to drive transcription.b.Prepare the in vitro translation reaction system

(2) ZSCAN21 mediates the pathogenic transcriptional induction of α-synuclein in cellular and animal models of Parkinson's diseaseCell death & diseaseMay 16, 2025Kozoriz A, Mora S, Damiano MA, Carballo-Carbajal I, Parent A, Kumarasinghe L, Vila M, Lassot I, Desagher S.was incubated for 1 h at 30 °C in 50 μl of the TNT® T7 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer. Equivalent amounts of the different forms of ZSCAN21

(3) Crosstalk interactions between transcription factors ERRα and PPARα assist PPARα-mediated gene expressionMolecular MetabolismJune 1, 2024Sofie J. Desmet, Jonathan Thommis, Tineke Vanderhaeghen, Edmee M.F. Vandenboorn, Dorien Clarisse, Yunkun Li, Steven Timmermans, Daria Fijalkowska, Dariusz Ratman, Evelien Van Hamme, et al.the TnT reticulocyte reaction (Promega cat #L1170, 2 h on 37 °C) or the TnT wheat germ extract (cat #L5030, 90′ on 30 °C), and added (20 μl/sample) to the beads solution, together with 5 μM GW or solvent control

(4) Environmental allergens trigger type 2 inflammation through ripoptosome activationNature immunologyOctober 1, 2021Brusilovsky M, Rochman M, Rochman Y, Caldwell JM, Mack LE, Felton JM, Habel JE, Porollo A, Pasare C, Rothenberg ME.proteins were produced using the TnT Coupled Wheat Germ Extract Transcription/Translation Systems (L5030; Promega) according to the manufacturer’s specifications. The resulting IL-33 proteins were qualitatively

(5) Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root GravitropismCurrent BiologyJanuary 11, 2021Maria Mar Marquès-Bueno et al.clonase,Thermofisher,Cat#12538120 TnT Coupled Wheat Germ Extract System,Promega / Thermofisher,Cat#L5030 Halo-tag magnetic beads,Promega / Thermofisher,Cat#7281

(6) Targeting the MLL complex in castration-resistant prostate cancerNature medicineApril 1, 2015Malik R, Khan AP, Asangani IA, Cieślik M, Prensner JR, Wang X, Iyer MK, Jiang X, Borkin D, Escara-Wilke J, et al.using the TNT® SP6 High-Yield Wheat Germ Reaction cell-free transcription and translation system (Cat.# L5030, Promega) following the manufacturer’s protocol. For each reaction, protein expression was confirmed

(7) Therapeutic targeting of BET bromodomain proteins in castration-resistant prostate cancerNatureJune 12, 2014Asangani IA, Dommeti VL, Wang X, Malik R, Cieslik M, Yang R, Escara-Wilke J, Wilder-Romans K, Dhanireddy S, Engelke C, et al.the fusion proteins were expressed using the cell-free transcription and translation system (Cat.# L5030, Promega) following the manufacturer's protocol. For each reaction, protein expression was confirmed

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