ClonePix 2 Mammalian Colony Picker from Molecular Devices

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ItemClonePix 2 Mammalian Colony Picker
CompanyMolecular Devices
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Catalog NumberClonePix 2
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Molecular Devices

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: www.moleculardevices.com

(1) Development of Antibody–Drug Conjugates Targeting L1CAM to Treat Metastatic CancerMolecular Cancer TherapeuticsJanuary 26, 2026Jin Suk Park, Carson Kenum, Lan He, Abdul G. Khan, Mary Ann Pohl, Thomas E. White, Sreekumar R. Kodangattil, Charles M. Rudin, Paul J. Balderes, Ivo C. Lorenz, et al.mouse L1CAM expressed by transiently transfected HEK293FT cells. Hybridomas were subcloned using the ClonePix2 (Molecular Devices), and clonal supernatants were tested for binding to human L1CAM by ELISA and flow cytometry

(2) Developing SEMA4A-directed CAR T cells to overcome low BCMA antigen density in multiple myelomaCancer CellDecember 8, 2025Francesco Di Meo, Francesca Albano, Annamaria Cesarano, Yunfei Wang, Brandon Kale, Kenneth Shain, Ariosto Silva, Noriyoshi Kurihara, Hirofumi Tenshin, David Jellyman, et al.bead-conjugated SEMA4A ECD antigen. Up to fifteen hybridoma clones were selected and subcloned by a ClonePix® 2 Colony Picker (Molecular Devices, CPII) in semi-solid methyl cellulose selection media containing fluorescently

(3) Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope ProteinBiotechnology and BioengineeringAugust 22, 2025Matthew Stuible, Sergio P. Alpuche‐Lazcano, Christian Gervais, Manon Ouimet, Julie Lippens, Martine Pagé, Audrey Morasse, Anna N. Moraitis, Yves Durocher(Thermo Scientific) and incubated for 6 days at 37°C, 5% CO₂. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐secretor hybridoma

(4) Metabolic engineering to facilitate anti-tumor immunityCancer CellMarch 10, 2025Tanya Schild, Patrick Wallisch, Yixuan Zhao, Ya-Ting Wang, Lyric Haughton, Rachel Chirayil, Kaitlyn Pierpont, Kevin Chen, Sara Nunes-Violante, Justin Cross, et al.155-545-071). Single colonies were picked and placed into culture with the aid of a robotic instrument (ClonePix 2, Molecular Devices, San Diego, CA). Supernatant from the monoclonal cultures were assessed by flow cytometry

(5) Bioprocessing considerations for generation of iPSCs intended for clinical application: perspectives from the ISCT Emerging Regenerative Medicine Technology working groupCytotherapyNovember 1, 2024Hannah W. Song, Jennifer N. Solomon, Fernanda Masri, Amanda Mack, Nisha Durand, Emmanuelle Cameau, Noushin Dianat, Arwen Hunter, Steve Oh, Brianna Schoen, et al.Clone pickers designed for hybridoma clones cultured in semi solid agar, adapted to mammalian cells" ClonePix2 Molecular Devices,"Developed for semisolid media, primarily used for cell line development. 10x faster

(6) Discovery of RXFP2 genetic association in resistant hypertensive men and RXFP2 antagonists for the treatment of resistant hypertensionScientific reportsJune 8, 2024Zhang SS, Larrabee L, Chang AH, Desai S, Sloan L, Wang X, Wu Y, Parvez N, Amaratunga K, Hartman AC, et al.colonies were allowed to grow for ~ 7 days post fusion after which the omnitray plates were imaged on the Clonepix 2 colony picker (Molecular Devices) and the IgG + hybridomas were picked into 96-well plates containing

(7) TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entryCellAugust 3, 2023Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, Calvaresi V, Martin SR, Roustan C, Cronin NB, et al.hybridomas were isolated using the Clonacell-HY Hybridoma Kit (Stemcell Technologies, Vancouver, BC) and Clonepix2 colony picker (Molecular Devices, Sunnyvale, CA), and supernatants were screened by FACS on HEK293 cells

(8) Validation of immunoassays for the Chlamydia trachomatis antigen Pgp3 using a chimeric monoclonal antibodyScientific ReportsMay 4, 2023Brook Goodhew, Xiaoling Tang, Jason Goldstein, Joo Lee, Diana Martin, Sarah GwynSwitzerland). After ten days of plating, clones grown in the presence of CloneDetect were screened by ClonePix 2 system (Molecular Devices, San Jose, California, USA) per the manufacturer’s instructions. A total of

(9) Development of HEK-293 Cell Lines Constitutively Expressing Flaviviral Antigens for Use in DiagnosticsMicrobiology SpectrumJune 29, 2022Jordan A. Powers, Benjamin Skinner, Brent S. Davis, Brad J. Biggerstaff, Lucy Robb, Elizabeth Gordon, William M. de Souza, Marcilio Jorge Fumagalli, Amanda E. Calvert, Gwong-Jen Changwere cultured for 7 to 14 days at 37°C with 5% CO2. Colonies were identified and selected using the ClonePix2 automated mammalian colony picker (Molecular Devices, CA, USA) according to the manufacturer’s instructions.

(10) Monoclonal antibodies to Cache Valley virus for serological diagnosisPLOS Neglected Tropical DiseasesJanuary 24, 2022Benjamin Skinner, Sierra Mikula, Brent S. Davis, Jordan A. Powers, Holly R. Hughes, Amanda E. Calvertfor 10 days at 37°C in 5% CO2. Clones expressing anti-mouse IgG were identified and picked with the ClonePix2 automated colony picker (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturer’s protocol.

(11) SARS-CoV-2 spike protein variant binding affinity to an angiotensin-converting enzyme 2 fusion glycoproteinsPloS oneJanuary 1, 2022Matthews AM, Biel TG, Ortega-Rodriguez U, Falkowski VM, Bush X, Faison T, Xie H, Agarabi C, Rao VA, Ju T.and placed in 96-well plates containing FreeStyle CHO Medium (Cat # 12651014, ThermoFisher) using the ClonePix 2 Mammalian Colony Picker as described by the manufacturer (Molecular Devices). The cell population from each clone was counted

(12) ZRC3308 Monoclonal Antibody Cocktail Shows Protective Efficacy in Syrian Hamsters against SARS-CoV-2 InfectionVirusesDecember 3, 2021Yadav PD, Mendiratta SK, Mohandas S, Singh AK, Abraham P, Shete A, Bandyopadhyay S, Kumar S, Parikh A, Kalita P, et al.IgG H + L specific, fluorescein (Molecular Devices, San Jose, CA, USA), for clonal selection using ClonePix2 (Molecular Devices, San Jose, CA, USA). After 7–10 days, based on high exterior mean fluorescent intensity,

(13) Therapy for Argentine hemorrhagic fever in nonhuman primates with a humanized monoclonal antibodyProceedings of the National Academy of Sciences of the United States of AmericaMarch 16, 2021Zeitlin L, Cross RW, Geisbert JB, Borisevich V, Agans KN, Prasad AN, Enterlein S, Aman MJ, Bornholdt ZA, Brennan MB, et al.heavy and light chains. Stable pools were selected with methionine sulfoximine and enriched using a ClonePix2 colony picker (Molecular Devices). ClonePix nonclonal isolates were grown and expanded in static culture,

(14) Rich production media as a platform for CHO cell line developmentAMB ExpressDecember 1, 2020Yong Jae Kim, Sang Kyul Han, Seongtae Yoon, Chan Wha Kimconditions for 48 h in the presence of HT. After 48 h, cells were either used for mini-pool assessment or ClonePix2 (Molecular Devices, San Jose, CA, USA) experiments. The cells were further cultured without HT and adding

(15) A kinase-deficient NTRK2 splice variant predominates in glioma and amplifies several oncogenic signaling pathwaysNature communicationsJune 12, 2020Pattwell SS, Arora S, Cimino PJ, Ozawa T, Szulzewsky F, Hoellerbauer P, Bonifert T, Hoffstrom BG, Boiani NE, Bolouri H, et al.Harvard Apparatus). Hybridomas secreting peptide specific antibody were identified and isolated using a ClonePix2 (Molecular Devices, CPII) colony picker. Antibodies from the picked clones were validated for peptide

(16) Transferrin Receptor Is a Specific Ferroptosis MarkerCell reportsMarch 10, 2020Feng H, Schorpp K, Jin J, Yozwiak CE, Hoffstrom BG, Decker AM, Rajbhandari P, Stokes ME, Bender HG, Csuka JM, et al.Approximately 4,750 hybridomas positive for IgG secretion were then identified and isolated using a ClonePix2 colony picker (Molecular Devices, CPII). Primary screening of the 4,750 clones was performed by indirect

(17) Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony SorterInternational Journal of Molecular SciencesOctober 16, 2019R. Ashton Lavoie, Alice di Fazio, Ruben G. Carbonell, Stefano Menegattipicking to be manually operated. To expand on this “cast-image-pick” approach, this work adapted a ClonePix 2 colony picker (Molecular Devices, Sunnyvale, CA) to fully automate the bead picking process with minimal

(18) New Monoclonal Antibodies for a Selective Detection of Membrane-Associated and Soluble Forms of Carbonic Anhydrase IX in Human Cell Lines and Biological SamplesBiomoleculesJuly 25, 2019Dovile Stravinskiene, Aiste Imbrasaite, Vilma Petrikaite, Daumantas Matulis, Jurgita Matuliene, Aurelija Zvirblienedays without moving to prevent cell scattering. Before procedure for hybridoma clones isolation, the ClonePix 2 system (Molecular Devices) was sanitized and calibrate according to manufacturer’s recommendations.

(19) Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand DiscoveryInternational journal of molecular sciencesApril 8, 2019Lavoie RA, di Fazio A, Blackburn RK, Goshe MB, Carbonell RG, Menegatti S.Lyophilized polyclonal human IgG was obtained from Athens Research (Athens, GA, USA). CloneMatrix for ClonePix 2 screening was generously provided by Molecular Devices (Sunnyvale, CA, USA). The model mAb production

(20) Production of a recombinant monoclonal antibody to Herpes Simplex Virus glycoprotein D for immunoaffinity purification of tagged proteinsJournal of Immunological MethodsFebruary 1, 2019Sara M. O'Rourke, Bin Yu, Javier F. Morales, Chelsea M. Didinger, David L. Alexander, Christopher Vollmers, Phillip W. Bermantranslation. 3.2. Stable CHO-S r34.1 clone selection Stable G418 resistant CHO-S lines were generated using a ClonePix2 (Molecular devices Sunnyvale, CA) robot, following electroporation of CHO-S cells with linearized vector

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