ClaI from New England Biolabs

Product Specs
ItemClaI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0197L
Quantity5000 units
ApplicationsDNA Manipulation
Concentration5 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

AT/CGAT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Neuronal hyperactivity becomes mTORC1 independent due to transcriptional changes in tuberous sclerosis complex disease modelsCell ReportsDecember 23, 2025Wardiya Afshar-Saber, Juan F. Ruiz, Isabel Gisser, Ziqin Yang, Leena Mehendale, Nicole A. Teaney, Rachel Hobson, Madison R. Glass, Truc T. Pham, Elizabeth Bainbridge, et al.(Addgene, #85139) was digested with SmaI (New England Biolabs R0141S) and ClaI (New England Biolabs R0197S). These three fragments were cleaned up using SPRI beads (Mag-Bind TotalPure NGS; Omega Bio-Tek; M1378–00),

(2) Transgenic expression of the human Glucose Transporter1 (GLUT1) gene locus reduces disease burden in Glut1 deficiency syndrome model miceNeurobiology of DiseaseOctober 1, 2025Maoxue Tang, Sasa Teng, Ashley Y. Kim, Yueqing Peng, Umrao R. MonaniRP11-125O1 (BACPAC Genomics, Emeryville, CA) containing the human GLUT1 locus was digested with ClaI (Cat.# R0197S, NEB), and a ~ 79 kb fragment harboring SLC2A1 and SLC2A1-DT gel extracted and purified. The fragment

(3) Mycobacterium Transcriptional Factor BlaI Regulates Cell Division and Growth and Potentiates β-Lactam Antibiotic Efficacy Against MycobacteriaMicroorganismsSeptember 25, 2025Junqi Xu, Mingjun Zhang, Fuling Xie, Junfeng Zhen, Yuerigu Abuliken, Chaoyun Gao, Yongdong Dai, Zhiyong Jiang, Peibo Li, Jianping Xievectors were digested with corresponding restriction endonucleases (NEB: BamHI, Cat. R0136; ClaI, Cat. R0197; NdeI, Cat. R0111; HindIII, Cat. R0104) at 37 °C for 2 h, then purified using the Omega Gel Extraction

(4) Efficient cell-free translation from diverse human cell typesJournal of Biological ChemistryJuly 1, 2025Jana Ziegelmüller, Nikolaos Kouvelas, Nino Schwaller, Priyanka Thambythurai, Alexander M. Hofer, Oliver Mühlemann, Evangelos D. Karousisplasmids were linearized using BsmFI (Cat No. R0572 L) for 3xFLAG-RLuc and 3xFLAG-HBB or ClaI (Cat No. R0197L) for SMG6-3xFLAG and the 3xFLAG-HBB reporter without a poly(A) in reactions containing 40 ng/μl DNA

(5) A multi-kingdom genetic barcoding system for precise clone isolationNature BiotechnologyMay 21, 2025Soh Ishiguro, Kana Ishida, Rina C. Sakata, Minori Ichiraku, Ren Takimoto, Rina Yogo, Yusuke Kijima, Hideto Mori, Mamoru Tanaka, Samuel King, et al.inserts through triple restriction enzyme digestion with BsrGI-HF (NEB, no. R3575S), ClaI (NEB, no. R0197S) and PvuI-HF (NEB, no. R3150S), confirming that 16 out of 16 clones contained the desired inserts. To

(6) A transcription factor module mediating C2 photosynthesis in the BrassicaceaeEMBO ReportsMay 1, 2025Patrick J Dickinson, Sebastian Triesch, Urte Schlüter, Andreas P M Weber, Julian M Hibberd,New England Biolabs,R3733S T4 DNA Ligase and buffer,ThermoFisher,EL0011 BSA,ThermoFisher,B14 Cla1,New England Biolabs,R0197S Xba1,New England Biolabs,R0145S NaOH,ThermoFisher,A16037.36 Ethanol (96%),Fisher Scientific,15552393 Acetic

(7) A homozygous human WNT11 variant is associated with laterality, heart and renal defectsDisease Models & MechanismsMay 1, 2025Henrike Berns, Damian Weber, Maximilian Haas, Zeineb Bakey, Magdalena Maria Brislinger-Engelhardt, Miriam Schmidts, Peter WalentekENST00000322563.8, NM_004626.3, CCDS8242) and subcloned into pCS108 (using Cla1 and EcoR1 enzymes; NEB, R0197 and R3101) to serve as templates for in vitro mRNA synthesis using the primers listed in Table S2. Xenopus

(8) A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformationMolecular CellMarch 6, 2025Raja H. Ali, Esteban A. Orellana, Su Hyun Lee, Yun-Cheol Chae, Yantao Chen, Jim Clauwaert, Alyssa L. Kennedy, Ashley E. Gutierrez, David J. Papke, Mateo Valenzuela, et al.Fisher Scientific,Cat# 60-24-2 BamHI,New England Biolabs,Cat# R0136S ClaI,New England Biolabs,Cat# R0197S Acil,New England Biolabs,Cat# R3160 BglII,New England Biolabs,Cat# R0144 Sodium chloride,Sigma-Aldrich,Cat#

(9) Transcriptomic analysis of coxsackievirus B3 infection in induced pluripotent stem cell-derived brain-like endothelial cellsJournal of VirologyJanuary 31, 2025Sarah F. Hathcock, Julia Mamana, Taryn E. Keyzer, Nadine Vollmuth, Mohammad-Reza Shokri, Henry D. Mauser, Robert N. Correll, Daryl W. Lam, Brandon J. Kim, Jon SineGFP–CVB3 plasmid was then linearized by digesting with ClaI restriction enzyme (New England BioLabs; R0197S). Linearized products were then subsequently used for in vitro transcription with the mMESSAGE mMACHINE T7 kit

(10) Repair of replication-dependent double-strand breaks differs between the leading and lagging strandsMolecular CellJanuary 2, 2025Michael T. Kimble, Aakanksha Sane, Robert J.D. Reid, Matthew J. Johnson, Rodney Rothstein, Lorraine S. Symington,New England Biolabs,Cat# R0521S HindIII,New England Biolabs,Cat# R0104S ClaI,New England Biolabs,Cat# R0197S SpeI-HF,New England Biolabs,Cat# R3133L "dATP, dTTP, dCTP, dGTP",ThermoFisher,Cat# R0181 Alpha factor,Genscript,Cat#

(11) Differential roles of lysosomal cholesterol transporters in the development of C. elegans NMJsLife Science AllianceOctober 1, 2024Amin Guo, Qi Wu, Xin Yan, Kanghua Chen, Yuxiang Liu, Dingfa Liang, Yuxiao Yang, Qunfeng Luo, Mingtao Xiong, Yong Yu, et al.site, which were then assembled with digestion products of Spe I (#3133S; NEB)– and Cla I–treated (#R0197S; NEB) pDD282 (#66823; Addgene) by Gibson Assembly Master Mix (90). Primers used are listed in Table

(12) A syngeneic spontaneous zebrafish model of tp53-deficient, EGFRvIII, and PI3KCAH1047R-driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapse in vivoeLifeJuly 25, 2024Alex Weiss, Cassandra D'Amata, Bret J Pearson, Madeline N Hayesenzyme (New England Biolabs, R0146S) while gfap:EGFP was linearized using ClaI (New England Biolabs, R0197S), according to the manufacturer’s protocol. Restriction enzymes were heat inactivated and linearized

(13) Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Increases Cell Proliferation and Drug ResistanceMethods in Molecular BiologyJuly 11, 2024Xiongjie Jin, Demetrius Moskophidis, Nahid F. MivechiEndonuclease (New England Biolabs, cat no. R0146L) ClaI Restriction Endonuclease (New England Biolabs, cat no. R0197L) SacI Restriction Endonuclease (New England Biolabs, cat no. R3156L) BglII Restriction Endonuclease

(14) Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoidsDevelopmental CellJune 1, 2024Adriano Bolondi, Benjamin K. Law, Helene Kretzmer, Seher Ipek Gassaloglu, René Buschow, Christina Riemenschneider, Dian Yang, Maria Walther, Jesse V. Veenvliet, Alexander Meissner, et al.R0145S NotI,New England Biolabs,Cat# R0189S BsmbI,New England Biolabs,Cat# R0739S ClaI,New England Biolabs,Cat# R0197S MegaX DH10B competent cells,Thermo Fisher Scientific,Cat# C6400-03 Software and Algorithms,Software

(15) Deep mutational scanning of Pneumocystis jirovecii dihydrofolate reductase reveals allosteric mechanism of resistance to an antifolatePLOS GeneticsApril 29, 2024Francois D. Rouleau, Alexandre K. Dubé, Isabelle Gagnon-Arsenault, Soham Dibyachintan, Alicia Pageau, Philippe C. Després, Patrick Lagüe, Christian R. LandrypAG416-GPD-ScDHFR were digested using ClaI enzyme in rCutSmart buffer (New England Biolabs, catalog #R0197) according to manufacturer’s instructions, and purified using magnetic beads as described above. 3xFLAG

(16) A novel in vivo system to study coral biomineralization in the starlet sea anemone, Nematostella vectensisiScienceMarch 15, 2024Brent Foster, Fredrik Hugosson, Federica Scucchia, Camille Enjolras, Leslie S. Babonis, William Hoaen, Mark Q. Martindalein frame into the pCS2+8CmCherry vector (RRID:Addgene_34935) using AscI (NEB #R0558) and ClaI (NEB #R0197) cut sites. The SpCARP1 insert was synthesized as a gene fragment by Twistbioscience (Twistbioscience.com)

(17) Cytoglobin regulates NO-dependent cilia motility and organ laterality during developmentNat CommunDecember 14, 2023Elizabeth R. Rochon, Jianmin Xue, Manush Sayd Mohammed, Caroline Smith, Anders Hay-Schmidt, Anthony W. DeMartino, Adam Clark, Qinzi Xu, Cecilia W. Lo, Michael Tsang, et al.the forward primer and an Xho1 site to the reverse primer. pCS2+ plasmid was digested with ClaI (NEB, R0197s) and Xho1 (NEB, R0146s) and ligated with the cygb2 PCR product. nos2b mRNA overexpression plasmid was

(18) Biomolecular condensates in fungi are tuned to function at specific temperaturesbioRxivNovember 30, 2023Benjamin M. Stormo, Grace A. McLaughlin, Logan K. Frederick, Ameya P. Jalihal, Sierra J Cole, Ian Seim, Fred S. Dietrich, Amy S. Gladfelterverified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA). Plasmids were linearized using NcoI (R0197, R3193, New England BioLabs, Waltham, MA, USA) at 37°C for 1 hr. Linearization was confirmed by running

(19) Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafishDevelopmental CellNovember 1, 2023Masahiro Shin, Hui-Min Yin, Yu-Huan Shih, Takayuki Nozaki, Daneal Portman, Benjamin Toles, Amy Kolb, Kevin Luk, Sumio Isogai, Kinji Ishida, et al.High ROX 500nM,3Cr Biosciences,001-0010 ISceI,New England Biolabs,R0694 ClaI,New England Biolabs,R0197 SacII,New England Biolabs,R0157 XhoI,New England Biolabs,R0146 NotI-HF,New England Biolabs,R3189 HindIII-HF,New England Biolabs

(20) Doxycycline-inducible Expression of Proteins at Near-endogenous Levels in Mammalian Cells Using the Sleeping Beauty Transposon SystemBIO-PROTOCOLOctober 20, 2023Karolina Zak, Costin N. Antonescureagents Restriction enzymes, e.g., NcoI and ClaI (New England Biolabs, catalog numbers: R0193L and R0197L, respectively)Competent DH5α E. coli (Thermo Fisher, catalog number: 18265017)D-MEM/F-12 (1×), liquid

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ClaI

ClaI from New England Biolabs

Sequence

New England Biolabs

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ClaI from New England Biolabs

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