Dynabeads Mouse CD4 from Thermo Fisher Scientific

Product Specs
ItemDynabeads Mouse CD4
CompanyThermo Fisher Scientific
Price Inquire
Catalog Number11445D
QuantityProcesses ~2x10^9 cells total
TypeAntibody Coated Bead
Sample TypeLymph Node, Blood, Spleen
PurityResearch Grade
Particle Size4.5 µm
ReactivityMouse
Cell TypeT Cells (CD4)

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506International journal of molecular sciencesMarch 17, 2025Suzuki M, Baillo A, Albarracin L, Elean M, Serda R, Suda Y, Namai F, Nishiyama K, Kitazawa H, Villena J.cells that include macrophages and dendritic cells (DCs) were then resuspended in DMEM medium and the Dynabeads™ Mouse CD4 Positive Isolation Kit (Invitrogen) and DynaMag™-2 (Invitrogen) were used to obtain macrophages considering

(2) Neutralizing IL-16 enhances the efficacy of targeting Aurora-A therapy in colorectal cancer with high lymphocyte infiltration through restoring anti-tumor immunityCell death & diseaseJanuary 30, 2024Yang SJ, Chang ST, Chang KC, Lin BW, Chang KY, Liu YW, Lai MD, Hung LY.collected from 8 to10-week-old BALB/c mice. CD4+ T cells were depleted using Dynabeads™ Mouse CD4 kit (11445D, Invitrogen), and CD8+ T cells were isolated using Dynabeads™ Untouched™ Mouse CD8 Cells Kit (11417D,

(3) IFN-γ-independent control of M. tuberculosis requires CD4 T cell-derived GM-CSF and activation of HIF-1αPLoS pathogensJuly 1, 2022Van Dis E, Fox DM, Morrison HM, Fines DM, Babirye JP, McCann LH, Rawal S, Cox JS, Stanley SA.hours postinfection, CD4 T cells were separated from BMDMs using CD4 (L3T4) Dynabeads from Invitrogen (11445D) according to the manufacturer’s protocol, and BMDMs were lysed in 1 mL TRIzol (Invitrogen). Total RNA

(4) Cancer-derived cholesterol sulfate is a key mediator to prevent tumor infiltration by effector T cellsInternational immunologyApril 20, 2022Tatsuguchi T, Uruno T, Sugiura Y, Sakata D, Izumi Y, Sakurai T, Hattori Y, Oki E, Kubota N, Nishimoto K, et al.lymph nodes of OTII (with or without DOCK2 expression) and OTI TCR transgenic mice by magnetic sorting with Dynabeads mouse CD4 (Invitrogen) followed by treatment with DETACHaBEAD™ mouse CD4 (Invitrogen), or by a CD8a+ T Cell Isolation

(5) Single‐cell transcriptomics uncovers an instructive T‐cell receptor role in adult γδ T‐cell lineage commitmentThe EMBO JournalMarch 1, 2022Sara Scaramuzzino, Delphine Potier, Robin Ordioni, Pierre Grenot, Dominique Payet‐Bornet, Hervé Luche, Bernard Malissenthymus Cells extracted from pooled thymus were depleted of CD4+, CD8+, and CD4+CD8+ T cells using Dynabeads Mouse CD4 and Dynabeads Mouse CD8 according to the manufacturer’s protocol (Invitrogen Life Technologies). Proper

(6) AMP-activated protein kinase alpha1 promotes tumor development via FOXP3 elevation in tumor-infiltrating Treg cellsiScienceJanuary 21, 2022An J, Ding Y, Yu C, Li J, You S, Liu Z, Song P, Zou MH.1640,CORNING,10-040-CV DMEM,CORNING,10-013-CV ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Dynabeads Mouse CD4+ CD25+ Treg isolation Kit,ThermoFisher,11463D eBioscience Foxp3/Transcription Factor Staining Buffer

(7) Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulationNature communicationsSeptember 1, 2021Hoefig KP, Reim A, Gallus C, Wong EH, Behrens G, Conrad C, Xu M, Kifinger L, Ito-Kureha T, Defourny KAY, et al.and regulatory T cells for RNA-IC Naïve CD4+ T cells were isolated by using Dyna- and Detachabeads (11445D and 12406D, Invitrogen) from spleens and mesenteric lymph nodes of 8–12 weeks old C57BL/6J mice. For

(8) Human Leukemia T-Cell Lines as Alternatives to Animal Use for Detecting Biologically Active Staphylococcal Enterotoxin Type BToxins (Basel)April 23, 2021Reuven Rasooly, Paula Do, Xiaohua He, Bradley HernlemIsolation of Murine CD4+ T-Cells For the positive and negative isolations of murine CD4+ T-cells, the Dynabeads Mouse CD4 L3T4 Positive Isolation kit and the Dynal Mouse CD4 Negative Isolation Kit were used according to the

(9) IL-33 drives the production of mouse regulatory T cells with enhanced in vivo suppressive activity in skin transplantationAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsMarch 1, 2021Kawai K, Uchiyama M, Hester J, Issa F.days) were processed into single cell suspensions. Cells were then enriched for CD4+ cells using the Dynabeads Mouse CD4 cells kit (Invitrogen, Thermo Fischer, cat 11415D) and subsequently isolated for CD25+ regulatory T cells

(10) T(H)17 cells require ongoing classic IL-6 receptor signaling to retain transcriptional and functional identityScience immunologyJuly 17, 2020Harbour SN, DiToro DF, Witte SJ, Zindl CL, Gao M, Schoeb TR, Jones GW, Jones SA, Hatton RD, Weaver CT.background. CD4 T cell preparation and culture CD4+ T cells were purified from pooled spleen and lymph nodes by Dynabeads Mouse CD4, followed by DETACHaBEAD Mouse CD4, according to the manufacturer’s instructions (ThermoFisher). Naïve

(11) Insulin-Like Growth Factors Are Key Regulators of T Helper 17 Regulatory T Cell Balance in AutoimmunityImmunityApril 14, 2020DiToro D, Harbour SN, Bando JK, Benavides G, Witte S, Laufer VA, Moseley C, Singer JR, Frey B, Turner H, et al.generate feeder cells, CD4+ T cells were depleted from total splenocytes using Dyna Beads (Invitrogen 11445D). CD4-depleted splenocytes were then exposed to 3000 rads. Human Igf (CU020), human desIgf1 (DU100),

(12)

Dendritic Cells Promote Treg Expansion but Not Th17 Generation in Response to Talaromyces marneffei Yeast Cells

Infection and Drug ResistanceMarch 11, 2020Yanping Tang, Hui Zhang, Haiguang Xu, Wen Zeng, Ye Qiu, Caimei Tan, Shudan Tang, Jianquan ZhangThe CD4+ T cells from the spleen singlecell suspensions were isolated by negative selection using the Dynabeads™ Mouse CD4 Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The purity of CD4+

(13) Dendritic Cells Promote Treg Expansion but Not Th17 Generation in Response to Talaromyces marneffei Yeast CellsInfect Drug ResistMarch 11, 2020Yanping Tang, Hui Zhang, Haiguang Xu, Wen Zeng, Ye Qiu, Caimei Tan, Shudan Tang, Jianquan ZhangCD4+ T cells from the spleen single-cell suspensions were isolated by negative selection using the Dynabeads™ Mouse CD4 Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The purity of CD4+

(14) Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5The Journal of experimental medicineNovember 5, 2018Buxadé M, Huerga Encabo H, Riera-Borrull M, Quintana-Gallardo L, López-Cotarelo P, Tellechea M, Martínez-Martínez S, Redondo JM, Martín-Caballero J, Flores JM, et al.(H-2Dd). T cells were isolated from the spleen and peripheral lymph nodes by positive selection using Dynabeads Mouse CD4 (Invitrogen; 114.45D) and DETACHaBEAD Mouse CD4 (Invitrogen; 124.06D) according to the manufacturer’s

(15) Identification of Functionally Relevant microRNAs in the Regulation of Allergic InflammationMethods in Molecular BiologyOctober 12, 2018Marlys S. Fassett, Heather H. Pua, Laura J. Simpson, David F. Steiner, K. Mark AnselNeon™ transfection 10 μl kit) 6. Dynabuffer 1x PBS supplemented with 2% fetal bovine serum (FBS) 7. Dynabeads™ Mouse CD4 kit (ThermoFisher) 8. DETACHaBEAD® (ThermoFisher) 9. miRIDIAN miRNA mimics and chemistry matched

(16) Differential IL-2 Expression Defines Developmental Fates of Follicular versus Non-follicular Helper T cellsScienceSeptember 14, 2018Daniel DiToro et al.lymph nodes) in two stages. First, CD8+ or CD4+ T-cells were isolated using Dynabeads (ThermoFisher 11445D), MACS Cell Separation (Miltyeni Biotec 130–104-454) or prepared by negative selection against CD8 or

(17) Th17 cells differentiated with mycelial membranes of Candida albicans prevent oral candidiasisFEMS Yeast ResearchMay 1, 2018Sonoko Tasaki, Tamaki Cho, Jun-ichi Nagao, Shojiro Ikezaki, Yuka Narita, Ken-ichi Arita-Morioka, Kanae Yasumatsu, Keita Toyoda, Hiroshi Kojima, Yoshihiko Tanakacells by a previously described method (Hashimoto et al.2017). Magnetic sorting was performed using Dynabeads Mouse CD4 (Invitrogen, Thermo Fisher Scientific, MA, USA) followed by treatment with DETACHaBEAD Mouse CD4 (Invitrogen).

(18) Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4⁺ T Cell DysfunctionImmunityDecember 19, 2017Wu J, Zhang H, Shi X, Xiao X, Fan Y, Minze LJ, Wang J, Ghobrial RM, Xia J, Sciammas R, et al.MOG35-55/CFA Emulsion PTX,Hooke Lab,Cat#EK-0113 Dynabeads Untouched Mouse CD4 Kit,Thermo Fisher,Cat#11416D Dynabeads Mouse CD4+CD25+ Treg Kit,Thermo Fisher,Cat#11463D RNeasy Mini Kit,Qiagen,Cat#74106 iScript™ Reverse Transcription

(19) The AP-1 transcription factor JunB is required for Th17 cell differentiationScientific reportsDecember 12, 2017Yamazaki S, Tanaka Y, Araki H, Kohda A, Sanematsu F, Arasaki T, Duan X, Miura F, Katagiri T, Shindo R, et al.cells were prepared from the spleen and lymph nodes of 6–9 week old mice by magnetic sorting using Dynabeads® Mouse CD4 (L3T4, Invitrogen) and DETACHaBEAD® Mouse CD4 (Invitrogen), as described previously47. The CD4+ T cells

(20) LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formationNature communicationsJuly 12, 2017Raab M, Lu Y, Kohler K, Smith X, Strebhardt K, Rudd CE.rested in growth medium for 2 days in the absence of growth factor. CD4+ T cells were purified using Dynabeads mouse CD4 (11415D–Thermo Fisher Scientific) according to the manufacturer's instructions (Invitrogen). All experiments

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Dynabeads Mouse CD4 from Thermo Fisher Scientific

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Dynabeads Mouse CD4 from Thermo Fisher Scientific

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