Protein L Magnetic Beads from MedChemExpress

Product Specs
ItemProtein L Magnetic Beads
CompanyMedChemExpress
Price $66.00Supplier Page View Company Product Page
Catalog NumberHY-K0205
Quantity1 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
TypeMagnetic Beads

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(1) KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppressioniScienceAugust 21, 2026Pengcheng Li, Chengxin Yu, Runshi Xie, Changsheng Huang, Qi Wu, Anyi Liu, Xiaowei She, Mao Li, Zejun Rao, Lang Liu, et al.washed three times with NP-40 lysis buffer, then incubated overnight at 4°C with primary antibodies (HY-K0205,MCE). The beads were subsequently rotated with the cell lysate, and immunoblot assays were performed. Cytoplasmic

(2) Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimpJournal of Biological ChemistryJuly 1, 2026Qian Feng, Maoshuai Fu, Wei Wang, Tingchu Wu, Junjie Nie, Zhihong Zheng, Yongzhen Zhao, Jude Juventus Aweya, Yueling Zhanganti-O-GlcNAc antibodies following PNGase F pretreatment to remove N-linked glycans. Protein L (MedChemExpress, HY-K0205) agarose beads were used to capture immune complexes. Eluted proteins were resolved by SDS-PAGE and

(3) Chemical knockdown of Keap1 and homoPROTAC-ing allergic rhinitisActa Pharmaceutica Sinica BMay 27, 2025Jianyu Yan, Tianyu Wang, Ruizhi Yu, Lijuan Xu, Hongming Shao, Tengfei Li, Zhe Wang, Xudong Cha, Zhenyuan Miao, Chengguo Xing, et al.(Santa Cruz, sc-365626) on a shaker at 4 °C for 24 h. Next, protein L magnetic beads (MedChemExpress, HY-K0205) were added and incubated overnight at 4 °C. After washing three times with precooled PBST (1 × PBS

(4) Citrullinated IGF2BP1 promotes rheumatoid synovial aggression via increasing the mRNA stability of SEMA3DCommun BiolJanuary 14, 2025Yang Qin, Li Liu, Yanwen Zhang, Xiaowei Chen, Jiani Zhang, Sunwang Ling, Jianguang Wang, Xinyu Yangovernight, respectively. On the second day, the proteins were incubated with protein L magnetic beads (HY-K0205, MCE) or protein A/G magnetic beads (HY-K0202, MCE) for 1 h. After that, the beads were separated by

(5) STK16 promoted colorectal cancer progress in a c-MYC signaling-dependent mannerMolecular MedicineApril 15, 2024Li Peng, Liu Guangshi, Lai Bijiang· Wusman, Li TaoUygur Autonomous Region. Primary antibodies were initially incubated with Protein L Magnetic Beads (HY-K0205; MCE) for 2 h at room temperature, and subsequently, cell lysis was combined with pre-treated beads

(6) Inhibition of CARM1-Mediated Methylation of ACSL4 Promotes Ferroptosis in Colorectal CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)December 1, 2023Feng S, Rao Z, Zhang J, She X, Chen Y, Wan K, Li H, Zhao C, Feng Y, Wang G, et al.for the Western blot assay. After centrifugation, the supernatant was incubated with magnetic beads (HY‐K0205; MCE) overnight at 4 °C. The proteins were eluted from the magnetic beads and collected for subsequent

(7) PRMT6 methylation of STAT3 regulates tumor metastasis in breast cancerCell Death & DiseaseOctober 9, 2023Qianzhi Chen, Qingyi Hu, Yan Chen, Na Shen, Ning Zhang, Anshu Li, Lei Li, Junjun Liimmunoprecipitation assays, primary antibodies were initially incubated with Protein L Magnetic Beads (HY-K0205; MCE) at room temperature for 2 h. The lysate was subsequently exposed to the pre-treated magnetic beads

(8) PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillanceScience advancesMay 26, 2023Huang C, Ren S, Chen Y, Liu A, Wu Q, Jiang T, Lv P, Song D, Hu F, Lan J, et al.three times with NP-40 lysis buffer, and then primary antibodies were incubated with magnetic beads (HY-K0205; MCE) overnight at 4°C, subsequently rotated with cell lysis and immunoblot assays. In vitro methylation

(9) Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγScientific ReportsApril 5, 2023Tianlong Zhang, Haoran Li, Shiwei Sun, Wuling Zhou, Tieqi Zhang, Yueming Yu, Qiang Wang, Minghai Wangdish. Next, 1 mL syringes were used to pump cells for 10 min. Next, 40 μL of Protein L Magnetic Beads (HY-K0205, MCE) were incubated with the corresponding antibody for 30 min at room temperature and washed four

(10) Serine/threonine-protein kinase STK24 induces tumorigenesis by regulating the STAT3/VEGFA signaling pathwayJournal of Biological ChemistryMarch 1, 2023Senyan Lai, Dao Wang, Wei Sun, Xiaonian CaoPro; BLT). For immunoprecipitation, primary antibodies were incubated with Protein L Magnetic Beads (HY-K0205; MCE) at room temperature for 2 h. The whole cell lysates were then incubated overnight with beads at

(11) Glycogen synthase kinase GSK3α promotes tumorigenesis by activating HIF1/VEGFA signaling pathway in NSCLC tumorCell Communication and SignalingDecember 1, 2022Xiaonian Cao, Wei Wu, Dao Wang, Wei Sun, Senyan Laichemiluminescence method. For immunoprecipitation, primary antibodies were incubated with Protein L Magnetic Beads (HY-K0205; MCE) at room temperature for 2 h, and then whole cell lysis was cultured with beads at 4 °C overnight,

(12) Alternative splicing of HSPA12A pre-RNA by SRSF11 contributes to metastasis potential of colorectal cancerClinical and translational medicineNovember 1, 2022Pan YJ, Huo FC, Kang MJ, Liu BW, Wu MD, Pei DS.antibodies overnight at 4°C followed by coincubation with 50 µl magnetic beads (MCE, catalog number: HY‐K0205) per pipe for 1 h. The next step was to clean the beads three times by washing buffer with 1% cocktail

(13) EZH2-triggered methylation of SMAD3 promotes its activation and tumor metastasisThe Journal of clinical investigationMarch 1, 2022Huang C, Hu F, Song D, Sun X, Liu A, Wu Q, She X, Chen Y, Chen L, Hu F, et al.by chemiluminescence. For IP, the indicated primary antibodies were incubated with magnetic beads (HY-K0205, MedChemExpress [MCE]) overnight at 4°C and subsequently rotated with cell lysis, followed by Western blot

(14) LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axisMolecular Medicine ReportsMarch 16, 2021Li Jiang, Chao Cheng, Wei Ji, Hao Wang, Quan Du, Xiaoqiao Dong, Junfei Shao, Wenhua YuInc.) at 4°C overnight, followed by incubating with 30 µl Magnetic beads (MedChemExpress; cat. no. HY-K0205) for 6–8 h at 4°C. Argonaute proteins are involved in the various steps of miRNA-mediated gene silencing

(15) Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal CardiomyocytesFrontiers in cardiovascular medicineJanuary 1, 2021Xu R, Kang L, Wei S, Yang C, Fu Y, Ding Z, Zou Y.against HA or isotype control immunoglobulin G (IgG) at 4°C overnight. Then, 50 μl magnetic beads (MCE, #HY-K0205) were added into the mixture and incubated for 4 h with rotation. After removing the surface attachments,

(16) NCOA4-Mediated Ferritinophagy Induces Ferroptosis and Enriches Ferritin-Containing EVs via Ferritin Phase Separation to Promote Mechanical Ventilation-Induced Pulmonary FibrosisJournal of advanced researchJanuary 1, 0001"Huang X, Feng J, Xu Q, Tang R, Peng Y, Yang W, Yang X, Xing S, Qian G, Gao Y, et al."Thermofisher, USA) and rabbit-IgG polyclonal antibody (2729, CST, USA) as control. Magnetic beads (HY-K0205, MCE, USA) were incubated with antibodies (500 µg of total protein per 1 µg of antibody) diluted in

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Protein L Magnetic Beads from MedChemExpress

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Protein L Magnetic Beads from MedChemExpress

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