Description
Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique.
Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms:
- In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size
- For monosaccharide separations, ligand exchange is the primary mechanism and involves the binding of hydroxyl groups of the sugars with the fixed counterion of the resin. Ligand exchange is affected by the nature of the counterion (Pb2+, Ca2+, etc.) and by the spatial orientation of the carbohydrate's hydroxyl groups
Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 µm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity.
Aminex columns are an industry standard for the analysis of carbohydrates, organic acids and alcohols. Bio-Rad offers a complete line of carbohydrate analysis columns optimized to provide high selectivity for a specific carbohydrate or carbohydrate class. Organic acid columns are used for simple, yet specific methods of food and beverage analysis. Research length (300 mm) columns provide high-resolution separations of complex carbohydrate mixtures in approximately 20 minutes, while the shorter columns provide efficient separations of specific carbohydrates in 3–5 minutes. Use our selection guide to choose the column that fits your application needs.