CHT Ceramic Hydroxyapatite from Bio-Rad

Product Specs
ItemCHT Ceramic Hydroxyapatite
CompanyBio-Rad
Price $985.00Supplier Page View Company Product Page
Catalog Number1570080

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) The E. coli DnaX clamp loader sharply bends DNA to load β-clamp at nicks and small gapsMol CellMay 14, 2026Fengwei Zheng, Nina Y. Yao, Roxana E. Georgescu, Meinan Lyu, Michael E. O'Donnell, Huilin Lisolubilizing in 6 M urea in buffer A. This solubilized material was passed through a column with 10 mL CHT ceramic hydroxyapatite Type I resin (Bio-Rad) equilibrated in buffer A + 6 M urea. The flow through was then loaded onto a

(2) Mass spectrometry reveals the evolutionary conservation of phycobiliprotein complexesNature CommunicationsFebruary 16, 2026Jaspreet K. Sound, Giorgio Bianchini, Thrupthi A. Ashok, Cecilia Rad-Menéndez, David H. Green, Patricia Sánchez-Baracaldo, Aneika C. Leneyphosphate pH 7.4 to remove any residual ammonium sulfate and the proteins loaded onto a self-packed CHT Ceramic Hydroxyapatite column (Type 1, 40 µm) (Bio-Rad) pre-equilibrated in 5 mM potassium phosphate pH 7.4. The absorbance

(3) Heterogeneity within phycobilisomes is highly orchestratedPhotosynth ResFebruary 9, 2026Jaspreet K. Sound, Maayan Suissa Szlejf, Hannah E. Wedgwood, Noam Adir, Aneika C. LeneyAPC and PC (αβ)3 complexes were then separated by anion exchange chromatography using a self-packed CHT Ceramic Hydroxyapatite column (Type 1, 40 μm) (Bio-Rad), pre-equilibrated in 5 mM potassium phosphate pH 7.4. Purification

(4) Robust and Reproducible Monoclonal Antibody Production Using a Transgenic Silkworm SystemInternational Journal of Molecular SciencesOctober 22, 2025Seiki Yageta, Yudai Nagata, Mamoru Shimizu, Natsumi Yasaka, Takuma Iwasa, Takushi Nakajima, Takeo Kuwata, Shuzo Matsushita, Masahiro Tomitaresultant 1C10K solution was loaded onto a ceramic hydroxyapatite chromatography column packed with 5 L of CHT Ceramic Hydroxyapatite XT resin (Bio-Rad Laboratories) that had been equilibrated with equilibration buffer (10 mM sodium phosphate,

(5) Crystal structure of the fungal mannosyltransferase Och1 reveals active site primed for N-glycan bindingPLOS OneJuly 31, 2025Emma T. R. Kelly, Dmitry Rodionov, Barry Sleno, Pedro A. Romero, Albert M. Berghuismethods Materials TOPO Cloning and Pichia pastoris Expression kits were obtained from Invitrogen. Macro-Prep (CHT) Ceramic Hydroxyapatite supports (type 1 20 µM) were obtained from Bio-Rad (Mississauga, Ont., Canada). DEAE-Tricacryl® M was

(6) A pilot study of healing critical-sized calvarial defects by LL-37-generated monoosteophilsFront Bioeng BiotechnolJuly 14, 2025Keith Le, Huinan Liu, Chaoxing Zhang, Zhuo Li, Tove Olafsen, Yuman Fong, John E. Shively, Zhifang ZhangCatalog# 12571-063) was purchased from Gibco (Montana, USA). Bio-Gel HT hydroxyapatite (catalog# 1300151), CHT ceramic hydroxyapatite (catalog# 1572000, 20 µm particle size and catalog#1584000, 40 µm particle size) were purchased from

(7) Harnessing defective interfering particles and lipid nanoparticles for effective delivery of an anti-dengue virus RNA therapyMolecular Therapy - Nucleic AcidsMarch 11, 2025Min-Hsuan Lin, Pramila Maniam, Dongsheng Li, Bing Tang, Cameron R. Bishop, Andreas Suhrbier, Lucy Wales- Earl, Yaman Tayyar, Nigel A.J. McMillan, Li Li, et al.buffer containing 2% (v/v) gelatin hydrolysate (Merck G0262) and 5% (v/v) sorbitol (Merck S1876) before CHT ceramic hydroxyapatite (Bio-Rad Laboratories) chromatographic purification of DENV DIPs. CHT chromatography column purification

(8) Mug20-Rec25-Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensatesNucleic Acids ResMarch 4, 2025Max F Wang, Meng-Yun Li, Ya-Ching Yang, Yu-Chien Chuang, Chieh-Yu Tsai, Mai-Chi Nguyen Binder, Lijuan Ma, Sheng-Wei Lin, Hung-Wen Li, Gerald R Smith, et al.100 mM KCl and 200 mM imidazole. The eluate was loaded on an 8-mL Tricorn column (Cytiva) packed with CHT ceramic hydroxyapatite Type I resin (Bio-Rad Laboratories) and fractionated through a linear gradient of 0–280 mM KH2PO4 in

(9) Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and is essential for erythrocyte invasionPLOS BiologySeptember 18, 2024David Anaguano, Opeoluwa Adewale-Fasoro, Grace W. Vick, Sean Yanik, James Blauwkamp, Manuel A. Fierro, Sabrina Absalon, Prakash Srinivasan, Vasant Muralidharanimmobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column. Fractions containing rMBP-RON11 were pooled and dialyzed into phosphate-buffered saline (PBS)

(10) Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) familyNature communicationsMay 30, 2023Deshmukh FK, Ben-Nissan G, Olshina MA, Füzesi-Levi MG, Polkinghorn C, Arkind G, Leushkin Y, Fainer I, Fleishman SJ, Tawfik D, et al.containing 10 mM MgCl2 using 10 kD Vivaspin 20 ml columns (GE Healthcare). Samples were then loaded onto a CHT ceramic hydroxyapatite column (Bio-Rad Laboratories Inc.); a linear gradient of 10–400 mM phosphate buffer was used for elution.

(11) Large-scale purification of functional AAV particles packaging the full genome using short-term ultracentrifugation with a zonal rotorGene TherapyMarch 28, 2023Mikako Wada, Naoya Uchida, Guillermo Posadas-Herrera, Hiromi Hayashita-Kinoh, Yuji Tsunekawa, Yukihiko Hirai, Takashi Okadaflow rate of 1.0 mL/min. A column (4.6 × 35 mm, Sugiyama Shoji Co., Ltd. Kanagawa, Japan) packed with CHT Ceramic Hydroxyapatite Type I, 40 m (Bio-Rad Laboratories Inc., Hercules, CA, USA) was equilibrated with 10 mM HEPES and 150

(12) RNA mis-splicing drives viral mimicry response after DNMTi therapy in SETD2-mutant kidney cancerCell ReportsJanuary 31, 2023Hong-Tao Li, H. Josh Jang, Krizia Rohena-Rivera, Minmin Liu, Hemant Gujar, Justin Kulchycki, Shuqing Zhao, Sandrin Billet, Xinyi Zhou, Daniel J. Weisenberger, et al.XP,BECKMAN COULTER,Cat# A63881 Pierce ChlP-grade Protein A/G Magnetic Beads,Thermo Scientific,Cat# 26162 CHT Ceramic Hydroxyapatite Type I 20 μm,Bio-Rad,Cat# 158–2000 5-Aza-2′-deoxycytidine,Millipore Sigma,Cat# A3656 Micrococcal Nuclease,Thermo Scientific

(13) The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombinationiScienceNovember 18, 2022Altmannova V, Spirek M, Orlic L, Jēkabsons A, Clarence T, Henggeler A, Mlcouskova J, Chaleil RAG, Matos J, Krejci L.[γ-32P], 3000 Ci/mmol",Hartmann Analytic,FP-501 HIS-Select Nickel Affinity Gel,Sigma-Aldrich,P6611 CHT Ceramic Hydroxyapatite,Bio-Rad,1584000 MonoQ,Cytiva,17516701 Superdex 200 Increase,Cytiva,28990944 Talon Superflow resin,Cytiva,28957502 Heparin Sepharose 6 Fast Flow

(14) Transient Inhibition of Trastuzumab-Tumor Binding to Overcome the “Binding-Site Barrier” and Improve the Efficacy of a Trastuzumab-Gelonin ImmunotoxinMol Cancer TherOctober 7, 2022Ping Chen, Brandon M. Bordeau, Yu Zhang, Joseph P. Balthasarusing a Ni2+-NTA column (Thermo Fisher Scientific, Waltham, MA, 88226) followed by a Bio-Scale mini CHT ceramic hydroxyapatite multimodal chromatography type I cartridge (BioRad, Hercules, CA, 7324324) (10). Trastuzumab was purchased

(15) Transient Inhibition of Trastuzumab–Tumor Binding to Overcome the “Binding-Site Barrier” and Improve the Efficacy of a Trastuzumab–Gelonin ImmunotoxinMolecular Cancer TherapeuticsOctober 7, 2022Ping Chen, Brandon M. Bordeau, Yu Zhang, Joseph P. Balthasarusing a Ni2+-NTA column (Thermo Fisher Scientific, Waltham, MA, 88226) followed by a Bio-Scale mini CHT ceramic hydroxyapatite multimodal chromatography type I cartridge (BioRad, Hercules, CA, 7324324) (10). Trastuzumab was purchased

(16) Conformational Rearrangements in the Redox Cycling of NADPH-Cytochrome P450 Reductase from Sorghum bicolor Explored with FRET and Pressure-Perturbation SpectroscopyBiology (Basel)March 25, 2022Bixia Zhang, ChulHee Kang, Dmitri R. Davydovto 2 mg mL−1, its solution was dialyzed against 5 mM potassium phosphate buffer and applied onto a CHT ceramic hydroxyapatite column (Bio-Rad, Hercules, CA, USA). The fraction containing SbCPR was eluted by a linear phosphate

(17) Mobile origin-licensing factors confer resistance to conflicts with RNA polymeraseCell reportsMarch 22, 2022Scherr MJ, Wahab SA, Remus D, Duderstadt KE.Affinity Gel,Sigma-Aldrich,Cat# A2220 Protino Glutathione Agarose 4B,Macherey-Nagel,Cat# 745500.10 CHT Ceramic Hydroxyapatite,Bio-Rad,Cat# 1582200 DYNAL Dynabeads KilobaseBINDER Kit,Thermo Fisher Scientific,Cat# 60101 "RNase Inhibitor, Murine

(18) Recombinant Tropomyosin from the Pacific Oyster (Crassostrea gigas) for Better DiagnosisFoodsJanuary 30, 2022Roni Nugraha, Thimo Ruethers, Aya C. Taki, Elecia B. Johnston, Shaymaviswanathan Karnaneedi, Sandip D. Kamath, Andreas L. Lopatawas diluted in buffer containing 5 mM NaPO4, 150 mM NaCl, pH 6.8, and loaded onto a Bio-scale Mini CHT Ceramic Hydroxyapatite Cartridges column (BioRad, Hercules, CA, USA). An increasing concentration of phosphate was used to

(19) Structural and functional characterization of the intracellular filament-forming nitrite oxidoreductase multiprotein complexNature MicrobiologySeptember 1, 2021Tadeo Moreno Chicano et al.8.0, and concentrated to a volume of less than 10 ml. The sample was subsequently loaded onto a 30-ml CHT ceramic hydroxyapatite column (Bio-Rad) which was equilibrated with ~75 mM KPi buffer, pH 8.0. Proteins were eluted with a

(20) Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in OsteoarthritisInternational Journal of Molecular SciencesJuly 13, 2021Isabel Olmos Calvo et al.was prepared from pooled freshly isolated HAS that was filtered with an IgM antibodies BioScale Mini CHT ceramic hydroxyapatite cartridge type II (BioRad, 7324332). Afterwards, 2 mL of pooled sterile serum per vial were frozen at

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