UNO Q1 Column from Bio-Rad

Product Specs
ItemUNO Q1 Column
CompanyBio-Rad
Price $1,963.00Supplier Page View Company Product Page
Catalog Number7200001

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

(1) Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivationNature structural & molecular biologySeptember 1, 2025Zhang L, Gordiyenko Y, Morgan T, Franco C, Tufegdžić Vidaković A, Zhang S.using ammonium sulfate followed by affinity purification with an 8WG16 antibody column and a further UNO Q1 column (Bio-Rad). The final porcine Pol II sample was stored in Pol II storage buffer (20 mM HEPES, pH 7.5,

(2) Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complexNat CommunJuly 1, 2025Luojia Zhang, Christopher Batters, Shintaro Aibara, Yuliya Gordiyenko, Kristina Žumer, Jana Schmitzová, Kerstin Maier, Patrick Cramer, Suyang Zhangbuffer, and eluted with 0.18 M buffer supplemented with 50 mM biotin. The eluate was loaded onto an UNO Q1 column (BioRad), washed with 0.18 M buffer, and eluted with a linear gradient to 0.5 M buffer. The peak fractions

(3) Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausingMolecular CellApril 4, 2024Bonnie G. Su, Seychelle M. Vosv1.6.1,"Goddard et al., 201878",https://www.cgl.ucsf.edu/chimerax/download.html Other,Other,Other UnoQ,Bio-Rad,7200001 HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography column,Cytiva,17116701 HisTrap HP His

(4) Role of the histone tails in histone octamer transferNucleic Acids ResearchMay 8, 2023Yahli Lorch, Roger D Kornberg, Barbara Maier-Daviscolumn volumes of TEV protease in 50 mM RSC buffer overnight at 4°C. RSC fractions were applied to a UNO Q1 column (20 ml, Bio-Rad) in 50 mM RSC buffer, washed with 100 mM RSC buffer and eluted with a gradient of 0.1–1

(5) The Allergenic Activity of Blo t 2, a Blomia tropicalis IgE-Binding MoleculeInternational Journal of Molecular SciencesMarch 14, 2023Ernesto Mondol, Karen Donado, Ronald Regino, Karen Hernandez, Dilia Mercado, Ana Carolina Mercado, Inés Benedetti, Leonardo Puerta, Josefina Zakzuk, Luis CaraballoFigure S2). Then, a second purification step was carried out using anion exchange chromatography in an UNO Q1 column (Cat. 720-0001, Bio-Rad, Hercules, CA, USA) adapted to a fast performance liquid chromatography (FPLC)

(6) Antimicrobial Potential of Microorganisms Isolated from the Bottom Sediments of Lake BaikalAntibiotics (Basel)July 30, 2021Olga Babich, Margarita Shevchenko, Svetlana Ivanova, Valery Pavsky, Maria Zimina, Svetlana Noskova, Veronika Anohova, Evgeny Chupakhin, Stanislav Sukhikhsubjected to chromatographic separation using an NGC HPLC chromatograph (Bio-Rad, Hercules, CA, USA) on an Uno-Q1 column (Bio-Rad, Hercules, CA, USA) at a gradient pH 2.5–8.9. The eluent was buffer solutions: phase A—citrate–phosphate

(7) Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate ContentMarine DrugsJuly 1, 2021Anna Andreeva, Ekaterina Budenkova, Olga Babich, Stanislav Sukhikh, Vyacheslav Dolganyuk, Philippe Michaud, Svetlana Ivanovahigh-performance liquid chromatography (HPLC) was used applying an HPLC NGC chromatograph (Bio-rad, Berkeley, CA, USA) on a Uno-Q1 column (Bio-rad, Berkeley, CA, USA) in the mode of gradient pH 2.5–8.9. The eluent comprised the following

(8) A widely distributed metalloenzyme class enables gut microbial metabolism of host- and diet-derived catecholseLifeFebruary 18, 2020Vayu Maini Rekdal, Paola Nol Bernadino, Michael U Luescher, Sina Kiamehr, Chip Le, Jordan E Bisanz, Peter J Turnbaugh, Elizabeth N Bess, Emily P Balskuscombined and injected onto the FPLC system described above for anion exchange chromatography using a UNO Q1 column (Bio-Rad, catalog# 720–0001) at a flow-rate of 1 mL/min. Fractions were eluted using a gradient of 0

(9) Stresses that Raise Np4A Levels Induce Protective Nucleoside Tetraphosphate Capping of Bacterial RNAMolecular CellSeptember 1, 2019Daniel J. Luciano, Rose Levenson-Palmer, Joel G. Belasco[32P]",Perkin Elmer,NEX053S015MC TALON Metal Affinity Resin,Clontech,635503 UNO Q1 column,Bio-Rad,7200001 TLC PEI cellulose F,Millipore,105579 3-acrylamidophenylboronic acid,"Ivanov et al., 2004",N/A Immobilon-Ny+,Millipore,INYC00010 Sephadex

(10) Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein CoronaBiomacromoleculesAugust 12, 2019Claudia Weber, Matthias Voigt, Johanna Simon, Ann-Kathrin Danner, Holger Frey, Volker Mailänder, Mark Helm, Svenja Morsbach, Katharina Landfestercentrifugation was injected into the system running with PBS at a flow rate of 1 mL min–1. A BioRad UNO Q1 column (BioRad, Munich, Germany) filled with Sephacryl S500-HR was used for separation. A multiwavelength detector

(11) Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panelClinical proteomicsJanuary 1, 2019Ahn SB, Sharma S, Mohamedali A, Mahboob S, Redmond WJ, Pascovici D, Wu JX, Zaw T, Adhikari S, Vaibhav V, et al.min. Strong anion exchange (SAX) peptide fractionation Digested peptides (100 µg) were fractionated using a UNO™ Q1 column (Bio-Rad, CA, USA) on a 1260 series HPLC (Agilent, Santa Clara, CA, USA). Fractionation was performed

(12) Histone Acetylation Inhibits RSC and Stabilizes the +1 NucleosomeMolecular CellNovember 1, 2018Yahli Lorch, Barbara Maier-Davis, Roger D. Kornbergcolumn volumes of TEV protease in 50 mM RSC buffer overnight at 4 °C. RS C fractions were applied to an UNO Q1 column (20 ml, Bio-Rad) in 50 mM RSC buffer, washed with 100 mM RSC buffer, and eluted with a gradient of 0.1–1

(13) A Novel RNA Phosphorylation State Enables 5â€² End-Dependent Degradation in Escherichia coliMolecular CellJuly 1, 2017Daniel J. Luciano, Nikita Vasilyev, Jamie Richards, Alexander Serganov, Joel G. Belascocell extracts by elution from TALON beads (Clontech) followed by anion-exchange chromatography on an UNO Q1 column (Bio-Rad). The purified protein was concentrated/exchanged into a buffer containing 10 mM Tris-HCl (pH

(14) Response to Metronidazole and Oxidative Stress Is Mediated through Homeostatic Regulator HsrA (HP1043) in Helicobacter pyloriJournal of BacteriologyFebruary 15, 2014Igor N. Olekhnovich, Serhiy Vitko, Meaghan Valliere, Paul S. HoffmanmM EDTA, 14 mM ␤ME), followed by dialysis against buffer B. The dialyzed sample was applied onto a UNO Q1 column (Bio-Rad Laboratories, Inc.). Proteins of interest were eluted with a 24-ml linear gradient of 0 to

(15) CELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSAmerican Journal of Biochemistry and BiotechnologyJanuary 1, 2014CierpkaLiquid Chromatography (FPLC). The purification process was performed with an anion exchange column (UNO Q1, column-material-N+(CH3)3, column-volume 1.3 mL, 20 mg max. protein load, BioRad) at a flow rate of 1 mL min−1.

(16) Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosaBMC MicrobiologyJuly 13, 2013Petra Tielen, Hubert Kuhn, Frank Rosenau, Karl-Erich Jaeger, Hans-Curt Flemming, Jost WingenderFractions containing the highest lipase activity (usually 15–20 fractions) were pooled and loaded onto an Uno-Q1 column (Bio-Rad, Munich, Germany), pre-equilibrated with buffer A (20 mM Tris–HCl pH 8.0, 100 mM NaCl) and

(17) Calcified Granulomatous Lung Lesions Contain Abundant Mycobacterium tuberculosis ComponentsMycobacterial DiseasesJanuary 1, 2013Tadatsune Iida Keisuke Uchidatuberculosis (ATCC25177) were fractionated on a Bio Logic DuoFlow 10 system by anion-exchange chromatography (UNO Q1 column; Bio-Rad, Hercules, CA) with a stepwise elution (based on conductivity, from 0 to 45 ms/cm in 10 equal

(18) Comparative analysis of cone and rod transducins using chimeric GÎ±-subunitsâ€ BiochemistryFebruary 28, 2012Kota N. Gopalakrishna, Kimberly K. Boyd, Nikolai O. Artemyevproteins were purified on His-bind Ni-NTA resin (Novagen) followed by an ion-exchange chromatography on Uno-Q1 column (BioRad). Fractions containing functional Gα-subunits were dialyzed against buffer containing 40% glycerol

(19) Hybrid Steered Molecular Dynamics-Docking: An Efficient Solution to the Problem of Ranking Inhibitor Affinities Against a Flexible Drug TargetMolecular InformaticsMay 16, 2011Katie L. Whalen, Kevin M. Chang, M. Ashley SpiesEluant was then diluted 10-fold with H2O and submitted to ion exchange chromatography using a BioRad Uno Q1 column on a BioRad BioLogic DuoFlow HPLC. Pooled fractions were then exchanged into protein storage buffer

(20) Purification and characterization of cytochrome c(6) from Acaryochloris marinaPhotosynthesis ResearchOctober 1, 2009Patrick D. Bell, Yueyong Xin, Robert E. Blankenshiptogether. For the final purification step, the pooled fractions were loaded onto a high resolution 2nd IEC UNO Q1 column (Bio-Rad). The sample was eluted by a 1 ml/min flow rate of a linear gradient from 0–0.5 M NaCl in 20

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UNO Q1 Column

UNO Q1 Column from Bio-Rad

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UNO Q1 Column from Bio-Rad

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