Macro-Prep High S Support from Bio-Rad

Product Specs
ItemMacro-Prep High S Support
CompanyBio-Rad
Price $53.00Supplier Page View Company Product Page
Catalog Number1580030

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tauNature CommunicationsJanuary 7, 2026Celine Park, Jaehun Jung, Yuri Hong, Haeun Yoo, Keunsang Yang, Jaehyeon Shin, Minsik Kim, Chan Lim, Ayoung Jeong, Seokyun Hong, et al.was incubated overnight at 4 °C. To further purify the protein, ion exchange chromatography (Bio-Rad #1580030) was performed to remove dimers and other undesired proteins. The protein elutes were concentrated using

(2) Unique Regulation of Sed-1 β-Lactamase in Citrobacter sedlakii: Insights on Resistance to Third-Generation CephalosporinAntibiotics (Basel)August 12, 2025Mako Watanabe, Ryuichi Nakano, Keizo Yamamoto, Akiyo Nakano, Yuki Suzuki, Kai Saito, Satoko Nakashima, Kentaro Endo, Kazuya Narita, Hisakazu Yanoresuspended in buffer A (20 mM Tris/HCl, pH 7.5) and sonicated. The cell-free extract was applied to a Macro-Prep High S Support column (Bio-Rad Laboratories, South Granville, NSW, Australia) and washed with buffer A. The enzymes

(3) Novel human recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in a glyco-engineered Escherichia coli strainHeliyonJune 30, 2024Luisa N. Pimentel-Vera, Alexander Rodríguez-López, Angela J. Espejo-Mojica, Aura María Ramírez, Carolina Cardona, Luis H. Reyes, Shunji Tomatsu, Thapakorn Jaroentomeechai, Matthew P. DeLisa, Oscar F. Sánchez, et al.buffer (25 mM sodium acetate, pH 5.0). rGALNSopt was purified by ion-exchange chromatography using a Macro-prep® High S-support column (Bio-rad, Hercules, CA, USA) in equilibrium buffer and eluted with a linear gradient of 0–0.5

(4) Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-β-lactamasesThe Journal of BiochemistryJanuary 1, 2023Keizo Yamamoto, Hideaki Tanaka, Genji Kurisu, Ryuichi Nakano, Hisakazu Yano, Hiromi Sakaibuffer A (20 mM Tris/HCl (pH 7.5), 50 μM ZnSO4) and sonicated. The cell-free extract was applied to a Macro-Prep High S Support column (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and washed with buffer A. The enzymes were eluted

(5) Cloning, Expression and Purification of Full-length Recombinant Ecarin and Comparing Its Expression and Function with Its Truncated FormIranian journal of pharmaceutical research : IJPRDecember 1, 2022Jafari Z, Bandehpour M, Gheflat S, Mohammadi N, Kazemi B.study. 3.4. Purification and Confirmation of the Ecarin Protein For the purification of Ecarin protein, a Macro-Prep High S Support (20 mL. Bio-Rad Laboratories) column was washed with 20 mM citrate (pH 5.0) buffer. The pH of the culture

(6) Isoforms of Cathepsin B1 in Neurotropic Schistosomula of Trichobilharzia regenti Differ in Substrate Preferences and a Highly Expressed Catalytically Inactive Paralog Binds CystatinFrontiers in Cellular and Infection MicrobiologyFebruary 26, 2020Hana Dvořáková, Roman Leontovyč, Tomáš Macháček, Anthony J. O'Donoghue, Ondřej Šedo, Zbyněk Zdráhal, Charles S. Craik, Conor R. Caffrey, Petr Horák, Libor Mikešexpressed according to a previously published protocol (Dvořák et al., 2005). A column packed with Macro-Prep High S Support medium (Bio-Rad) was used for pre-purification from yeast media by cation exchange chromatography. TrCB1-containing

(7) Thermosensitive PBP2a requires extracellular folding factors PrsA and HtrA1 for Staphylococcus aureus MRSA β-lactam resistanceCommunications BiologyDecember 1, 2019Mélanie Roch, Emmanuelle Lelong, Olesya O. Panasenko, Roberto Sierra, Adriana Renzoni, William L. Kelleyextensively dialyzed to remove residual imidazole in Buffer A and then concentrated by application to a 3 ml Macro-Prep High S Support (Bio-Rad) column equilibrated in Buffer A. PBP2a was step eluted in 0.4 M NaCl, dialyzed in Buffer A

(8) Identification of the iduronate-2-sulfatase proteome in wild-type mouse brainHeliyonMay 10, 2019Carolina Cardona, Eliana Benincore, Natalia Pimentel, Luis H. Reyes, Camilo Patarroyo, Alexander RodrÃguez-LÃ³pez, M. Martin-Rufian, Luis Alejandro Barrera, Carlos J. AlmÃ©ciga-DÃazCentrifugal filter (Millipore). The rhIDS was purified by using a cation exchange chromatography with a Macro-Prep High S support column (Bio-rad, Hercules, CA, USA) equilibrated with 25 mM potassium acetate, pH 5.0 ± 0.2, and eluted

(9) Type II restriction modification system in Ureaplasma parvum OMC-P162 strainPLOS ONEOctober 16, 2018Heng Ning Wu, Yukiko Nakura, Michinobu Yoshimura, Ourlad Alzeus Gaddi Tantengco, Makoto Nomiyama, Toshimitsu Takayanagi, Tomio Fujita, Kiyoshi Yasukawa, Itaru Yanagihara4°C. Then, the supernatant was diluted 10-fold with buffer A (50 mM Tris-HCl, pH 7.5) and loaded on a Macro-Prep High S Support column (Bio-Rad, CA, USA) equilibrated with 1 ml of buffer A, washed with 2 ml of buffer A, and eluted

(10) Proteome profiling of bovine follicular fluid-specific proteins and their effect on in vitro embryo developmentJournal of Veterinary Medical ScienceMay 3, 2017Saranya SATITMANWIWAT, Chinarat CHANGSANGFAH, Tassanee FAISAIKARM, Kornkanok PROMTHEP, Sujira THAMMAWUNG, Kulnasan SAIKHUN, Kampon KAEOKETthe bFF proteins To fractionate the basic proteins, the dialyzed protein samples were loaded into a Macro-Prep High S support in a 5-ml column (Bio-Rad, Hercules, CA, U.S.A.) equilibrated in 10 mM Tris buffer (pH 7.0). The bound

(11) Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial CellsMolecular & cellular proteomics : MCPApril 1, 2017Worthington J, Spain G, Timms JF.resuspended in SCX loading buffer (5 mm ammonium acetate, 25% ACN, 0.1% FA) and fractionated by SCX (Macro-Prep High S Support; Bio-Rad; Hemel Hempstead, UK) into five fractions by batchwise elution: flow-through and 15, 30, 60,

(12) Nitric oxide and cytokine production by glial cells exposed in vitro to neuropathogenic schistosome Trichobilharzia regentiParasites & VectorsDecember 1, 2016Tomáš Macháček, Lucie Panská, Hana Dvořáková, Petr Horákpastoris (Invitrogen) as described previously [13, 18]. For purification of the recombinant cathepsins, Macro-Prep High S Support (Bio-Rad) and fast protein liquid chromatography (BioLogic HR system, BioRad, Hercules, California,

(13) Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastorisUniversitas ScientiarumSeptember 29, 2016Angela Johana Espejo Mojica, Angela Rocío Mosquera, Edwin Alexander Rodríguez-López, Dennis Johana Díaz, Laura Milena Betrán, Francy Liliana Hernández, Carlos Javier Alméciga Díaz, Luis Alejandro Barreraor Macro-Prep DEAE Support (Bio-Rad Laboratories) for rhHex-A, while rhHex-B was purified by using Macro-Prep High S Support. Protein elution was performed by a 0 to 0.5 M NaCl linear gradient. Fractions with enzyme activity

(14) Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastorisScientific ReportsSeptember 1, 2016Alexander RodrÃguez-LÃ³pez, Carlos J. AlmÃ©ciga-DÃaz, Jhonnathan SÃ¡nchez, Jefferson Moreno, Laura Beltran, Dennis DÃaz, Andrea Pardo, Aura MarÃa RamÃrez, Angela J. Espejo-Mojica, Luisa Pimentel, et al.recombinant GALNS produced in E. coli30. Briefly, a cation exchange chromatography was performed with a Macro-Prep High S support column (Bio-rad, Hercules, CA, USA), equilibrated with 25 mM potassium acetate, pH 4.5, and eluted with

(15) Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purificationFrontiers in Plant ScienceAugust 31, 2015Laura M. López-Castillo, Janet A. I. López-Arciniega, Armando Guerrero-Rangel, Silvia Valdés-Rodríguez, Luis G. Brieba, Silverio García-Lara, Robert Winklerand subsequently centrifuged at 27,150 g for 30 min. The supernatant was collected, ﬁltered and then loaded on a Macro-Prep High S Support column (1.5 × 14.1 cm; Bio-Rad, Hercules, CA, USA). Protein was eluted with a linear gradient from 0

(16) Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragmentHuman Vaccines & ImmunotherapeuticsFebruary 19, 2015Terence L Kirley, Andrew B Normanincubation with a strong cation exchange resin (for 20 mg antibody digest used 1.5 ml bed volume of BioRad Macro-Prep High S Support (cat. 156-0030)) for 5 minutes at 22°C, the resin beads were poured into a column, washed extensively

(17) Selection of scFv Antibody Fragments Binding to Human Blood versus Lymphatic Endothelial Surface Antigens by Direct Cell Phage DisplayPloS oneJanuary 1, 2015Keller T, Kalt R, Raab I, Schachner H, Mayrhofer C, Kerjaschki D, Hantusch B.alkylate the proteins. Subsequent trypsin digestion was performed overnight at 37°C. Cation-exchange Macro-Prep High S Support columns (Bio-Rad) were used to clean the samples. Digested peptides were separated by nano-LC using

(18) Partial characterisation of peptides inhibiting Listeria growth in two Alpine cheesesDairy Science & TechnologyJanuary 1, 2014Phuong Nguyen Thi, Coralie Dupas, Isabelle Adt, Pascal Degraeve, Mélanie Ragon, May-Farah Missaoui, Enrico Novelli, Severino Segato, Dong Phan The, Nadia Oulahalvolume 30 mL). This preparation was then loaded onto a strong cation-exchanger 15-mL bed volume column (Macro-Prep High S support, Bio-Rad Laboratories, Marnesla-Coquette, France), equilibrated with 50 mmol.L−1 citrate buffer, pH

(19) Characterization and application of enterocin RM6, a bacteriocin from Enterococcus faecalisBioMed Research InternationalJune 13, 2013En Huang, Liwen Zhang, Yoon-Kyung Chung, Zuoxing Zheng, Ahmed E. Yousefsupernatant was adjusted to pH 6.5 using 1.0 N NaOH and passed through a cationic exchange column (Macro-Prep High S support; Bio-Rad, Hercules, CA, USA) that was equilibrated with phosphate buffer (50 mM, pH 6.5). Enterocin

(20) CYP261 enzymes from deep sea bacteria: A clue to conformational heterogeneity in cytochromes P450Biotechnology and Applied BiochemistryJanuary 25, 2013Dmitri R. Davydov, Elena V. Sineva, Nadezhda Y. Davydova, Douglas H. Bartlett, James R. HalpertmM Na-Hepes buffer (pH 7.4) containing 10% glycerol. The diluted solution was applied to a column of Macro-Prep High S Support resin (Bio-Rad Laboratories, Hercules, CA; 1 ml of the gel per 40 nmol P450) equilibrated with 10 mM

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