UNO S6 Column from Bio-Rad

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ItemUNO S6 Column
CompanyBio-Rad
Price $4,355.00Supplier Page View Company Product Page
Catalog Number7200023

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

(1) Small heat shock protein HSPB8 interacts with a pre-fibrillar TDP43 low complexity domain species to delay fibril formationBiophysical ChemistryMay 1, 2026Khaled M. Jami, Katherine E. Corbett, Daniel C. Farb, Kayla M. Osumi, Catelynn C. Shafer, Sophie Criscione, Dylan T. Murraywidth dialysis tubing. The protein was then syringe filtered (sterile 0.22μm, PES) and loaded onto an UNO S6 Column (Bio-Rad) equilibrated in CEX buffer using a Bio-Rad NGC Quest 10 Plus Chromatography system. Purified

(2) RNA polymerase II partitioning is a shared feature of diverse oncofusion condensatesCellJuly 10, 2025Heankel Lyons, Prashant Pradhan, Gopinath Prakasam, Shubham Vashishtha, Xiang Li, Mikayla Eppert, Christy Fornero, Vanina T. Tcheuyap, Kathleen McGlynn, Ze Yu, et al.Poly-Prep® Chromatography Columns,Bio-Rad,7311550 ENrich SEC 650 10 × 300 column,Bio-Rad,7801650 UNO S6 column,Bio-Rad,7200023 "Stellaris® FISH Probes, Custom Assay with Quasar® 670 Dye",LGC Biosearch Technologies,SMF-1065-5 Stellaris® RNA FISH Hybridization Buffer

(3) Small heat shock protein HSPB8 interacts with a pre-fibrillar TDP43 low complexity domain species to delay fibril formationbioRxivFebruary 1, 2025Khaled M. Jami, Daniel C. Farb, Kayla M. Osumi, Catelynn C. Shafer, Sophie Criscione, Dylan Thomas Murraywidth dialysis tubing. The protein was then syringe filtered (sterile 0.22 μm, PES) and loaded onto an UNO S6 Column (Bio-Rad) equilibrated in CEX buffer using a Bio-Rad NGC Quest 10 Plus Chromatography system. Purified

(4) Disorder-mediated interactions target proteins to specific condensatesMolecular CellSeptember 19, 2024Nancy De La Cruz, Prashant Pradhan, Reshma T. Veettil, Brooke A. Conti, Mariano Oppikofer, Benjamin R. Sabariinhibitor cocktail). mEGFP-MED1IDR was purified by cation exchange chromatography on UNO S6 column (Bio-Rad 7200023) using Buffer A (50 mM Tris, 5 mM BME) and Buffer B (50 mM Tris, 5 mM BME, 1M NaCl). Fractions (0.5

(5) Ribosomal Protein S12 Hastens Nucleation of Co-Transcriptional Ribosome AssemblyBiomoleculesJune 6, 2023Margaret L. Rodgers, Yunsheng Sun, Sarah A. Woodsonchange. The dialyzed protein was cleared by centrifugation and filtered (0.45 μm), then applied to a UNO-S6 column (Bio-Rad, Hercules, CA, USA) and eluted with a 0–40% linear gradient of 1 M KCl in buffer D. Half of

(6) Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17BiomoleculesMay 30, 2020Kumudie Jayalath, Sean Frisbie, Minhchau To, Sanjaya Abeysirigunawardenafinal KCl concentration of the labeling reaction was adjusted to 20 mM before passing through a BioRad Uno S6 column to remove the unreacted dye. Fluorescently labeled RsuA: G127C was eluted with a KCl gradient. Isolated

(7) Detecting and Characterizing the Kinetic Activation of Thermal Networks in Proteins: Thermal Transfer from a Distal, Solvent-Exposed Loop to the Active Site in Soybean LipoxygenaseThe Journal of Physical Chemistry BOctober 17, 2019Jan Paulo T. Zaragoza, Andy Nguy, Natalie Minnetian, Zhenyu Deng, Anthony T. Iavarone, Adam R. Offenbacher, Judith P. KlinmanRPM for 20 min and passed through a 0.45 μm filter to remove insoluble debris, and then loaded to an UNO S6 column (Bio-Rad) using a 150-mL Superloop (GE Healthcare). The column was washed with 20 mM Bis-Tris (pH 5.8)

(8) Nature of Amorphous Hydrophilic Block Affects Self-Assembly of an Artificial Viral Coat PolypeptideBiomacromoleculesOctober 14, 2019Lione Willems, Larissa van Westerveld, Stefan Roberts, Isaac Weitzhandler, Carlos Calcines Cruz, Armando Hernandez-Garcia, Ashutosh Chilkoti, Enrico Mastrobattista, John van der Oost, Renko de Vrieschromatography. For the cation-exchange chromatography, the soluble cleared lysate was loaded onto a UNO S6 column (Bio-Rad) connected to a BioLogic DuoFlow chromatography system supplied with a QuadTec detector from

(9) The MICALs are a Family of F-actin Dismantling Oxidoreductases Conserved from Drosophila to HumansScientific ReportsDecember 1, 2018Heng Wu, Hunkar Gizem Yesilyurt, Jimok Yoon, Jonathan R. Termanglycerol, 1 mM DTT), the samples containing the MICAL-1redoxCH protein were loaded into a MonoS column (Uno S6 column [Bio-Rad] or Mono S 5/50 GL Column [GE Healthcare]), washed with Buffer S-A (20 mM NaPO4, pH7.5, 10

(10) Effect of a K72A Mutation on the Structure, Stability, Dynamics, and Peroxidase Activity of Human Cytochrome cBiochemistryJuly 5, 2017Shiloh M. Nold, Haotian Lei, Tung-Chung Mou, Bruce E. Bowlerat −80 °C until used. Prior to experiments, WT and K72A Hu Cytc were purified by HPLC using a BioRad UNO S6 column, as previously decribed.20 Biochemistry. Author manuscript; available in PMC 2017 August 21. Nold

(11) A simple and efficient method for generating high-quality recombinant Mical enzyme for in vitro assaysProtein Expression and PurificationNovember 1, 2016Heng Wu, Ruei-Jiun Hung, Jonathan R. Termanand AKTA Purifier UPC 10 were from GE Healthcare Bio-Sciences Corporation (Piscataway, NJ, USA). The Uno S6 column was obtained from Bio-Rad Company (Hercules, CA, USA). Molecular Biology and Protein Expression In

(12) Disruption of a hydrogen bond network in human versus spider monkey cytochrome c affects heme crevice stabilityJournal of Inorganic BiochemistryMay 1, 2016Matthew E. Goldes, Margaret E. Jeakins-Cooley, Levi J. McClelland, Tung-Chung Mou, Bruce E. Bowlerare 9 – 10 mg per liter of 2x YT culture. Aliquots were thawed and purified by HPLC using a Bio-Rad UNO S6 column immediately in advance of experiments. The following gradient at a flow rate of 3.0 mL/min was used

(13) Differential effects of ribosomal proteins and Mg2+ ions on a conformational switch during 30S ribosome 5â€²-domain assemblyRNANovember 1, 2015Sanjaya C. Abeysirigunawardena, Sarah A. WoodsonmM 2-mercaptoethanol. The final KCl concentration was adjusted to 20 mM before loading on a BioRad Uno-S6 column to remove excess unreacted dye, followed by overnight dialysis against 80 mM K-HEPES pH 7.6, 1 M KCl,

(14) Kinetic Detection of Orthogonal Protein and Chemical Coordinates in Enzyme Catalysis: Double Mutants of Soybean LipoxygenaseBiochemistrySeptember 8, 2015Sudhir C. Sharma, Judith P. Klinman20 mM BIS-TRIS (pH 6.0) to remove salt. Protein was then concentrated and further purified using an UNO S6 column (Bio-Rad) with a 210 mL stepwise gradient [A consisting of 0 mM NaCl in 20 mM BIS-TRIS (pH 6.0) and

(15) The response of Ω-loop D dynamics to truncation of trimethyllysine 72 of yeast iso-1-cytochrome c depends on the nature of loop deformationJBIC Journal of Biological Inorganic ChemistryJuly 1, 2015Levi J. McClelland, Sean M. Seagraves, Md. Khurshid Alam Khan, Melisa M. Cherney, Swati Bandi, Justin E. Culbertson, Bruce E. Bowlerwere purified by cation-exchange HPLC using an Agilent Technologies 1200 series HPLC with a BioRad UNO S6 Column (catalog no. 720-0023). After collection of the iso-1-Cytc peak, samples were concentrated by ultrafiltration

(16) An improved surface passivation method for single-molecule studiesNature MethodsDecember 1, 2014Boyang Hua et al.single cysteine residues were over-expressed and purified by cation exchange chromatography using an UNO S6 column (BioRad) as described by Culver and Noller.24 Isolated proteins were dialyzed overnight into storage

(17) Dynamics of the His79-heme Alkaline Transition of Yeast Iso-1-cytochrome c Probed by Conformationally-gated Electron Transfer with Co(II)bis(terpyridine)â€ Journal of the American Chemical SocietyAugust 28, 2013Melisa M. Cherney, Carolyn C. Junior, Bryan B. Bergquist, Bruce E. Bowlerexperiments, protein was thawed and purified to homogeneity by HPLC (Agilent 1200 series) using a BioRad UNO S6 column as described previously.44 Co(terpy)22+-mediated Gated ET Measurements [Co(2,2′:6,2″-terpyridine)2]2+

(18) Identification and Quantification of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Cells by Liquid Chromatography/Isotope-Dilution Tandem Mass SpectrometryPLoS ONEJuly 29, 2013Güldal Kirkali, Pawel Jaruga, Prasad T. Reddy, Alessandro Tona, Bryant C. Nelson, Mengxia Li, David M. Wilson, Miral Dizdaroglurecombinant hAPE1 was immediately purified from the clarified extracts using sequential UNO Q12 and UNO S6 column (BioRad) chromatography as outlined previously [27]. The Q51H and G241R variant of hAPE1 were produced

(19) Binding properties of the regulatory domains in Manduca sexta hemolymph proteinase-14, an initiation enzyme of the prophenoloxidase activation systemDevelopmental & Comparative ImmunologyMarch 1, 2010Yang Wang, Haobo Jiangcombined, diluted with eight volumes of S buffer (50 mM sodium acetate, pH 4.8), and then loaded onto an UNO™ S6 column using a BioLogic DuoFlow System (Bio-Rad). Following washing, bound proteins were eluted with a linear

(20) Structural basis of HIV-1 activation by NF-kappaB - a higher-order complex of p50:RelA bound to the HIV-1 LTRJournal of Molecular BiologyOctober 16, 2009James C. Stroud, Amy Oltman, Aidong Han, Darren L. Bates, Lin Chenbeads (Qiagen) following the manufacturer's protocol. The eluted protein was further purified on an UNO S6 column (Bio-Rad) with a 10% to 30% NaCl gradient (10mM HEPES, pH 7.7, 5mM β-mercaptoethanol). Both p50 and

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UNO S6 Column

UNO S6 Column from Bio-Rad

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UNO S6 Column from Bio-Rad

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