Protein A Agarose Beads from Cell Signaling Technology

Product Specs
ItemProtein A Agarose Beads
CompanyCell Signaling Technology
Price $93.00 Inquire
Catalog Number9863P
Quantity200 µl
ApplicationsIP
Research AreaRelated Products
TypePrecast Gels / Media / Resins / Beads, Resins, Beads
ModeAntibody Purification
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) BxbI-mediated insertion of a 77kb human RET sensitive haplotype into the mouse genome to generate a humanized model of Hirschsprung diseasebioRxivJuly 6, 2026Ryan D. Fine, Benjamin E. Low, Jarod Rollins, Jon M. Laurent, Michael V. Wiles, Aamir Zuberi, Jef D. Boeke, Aravinda ChakravartiCell Signalling) and allowed to incubate with rotation overnight at 4°C. 40uL of lysis buffer-washed Protein A agarose beads (Thermo Scientific) were then added to the solution and incubated for an additional 4 hrs. Cold lysis

(2) UBA1-CDK16: A female-specific chimeric RNA emerging through evolution and involved in immune regulationSci AdvMay 29, 2026Xinrui Shi, Loryn Blackburn, Sandeep Singh, Martyna Glowczyk-Gluc, Anam Tajammal, Shafaque Zahra, Shailesh Kumar, Robert Cornelison, Chen Liang, Fujun Qin, et al.sonication, nuclei were cleared by centrifugation and subjected to preclearance by incubating with Protein A agarose beads (Cell Signaling). Precleared cell lysates were separately incubated with CTCF or nonspecific immunoglobulin

(3) MPND Loss Epigenetically Activates TGFβ/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non–Small Cell Lung CancerCancer ResMay 26, 2026Jianfeng Yu, Jieru Zhang, Jun Zhang, Zhenqi Bai, Yunqing Zhang, Zi’an Liu, Lifan Su, Ye Yang, Yixin Lian, Wei Gu, et al.Cell Signaling Technology, RRID: AB_2616029). Protein–DNA complexes were precipitated by incubation with saturated protein A agarose beads for an additional 2 hours at 4°C. After extensive washing, immunoprecipitated complexes were eluted

(4) HDAC7 controls anti-viral and anti-tumor immunity by CD8+ T cellsFront ImmunolMay 12, 2026Cansu Yerinde, Jacqueline Keye, Hsiang-Jung Hsiao, Sibel Durlanik, Inka Freise, Franziska Nowak, Marilena Letizia, Stephan Schlickeiser, Benedikt Obermayer, Adrian Huck, et al.protein A beads and primary antibodies (anti-H3K9K14, Cell Signaling Technologies) control IgG. Next day, protein A agarose beads were washed with low- and high-salt buffers followed by DNA elution by incubation at 30 °C for 15 min.

(5) MEK interactions tune RAF kinase sensitivity to conformation-selective inhibitionNature Chemical BiologyMay 12, 2026Ethan G. Stoddard, B. Gayani K. Perera, Linglan Fang, Daniel S. Brush, Yuhao Zhong, Zachary E. Potter, Jessica J. Simon, Martin Golkowski, Dustin J. Maly(Invitrogen, cat. no. 44-504G, 1:500 dilution) Protein A Agarose Beads (Cell Signaling Technology, cat. no. 9863) Protein G Agarose Beads (Cell Signaling Technology, cat. no. 37478) 14-3-3 (pan) E9S9M Rabbit mAb (Cell Signaling Technology

(6) Targeting Cancer-Associated PCNA with AOH1996 Induces Mitotic Catastrophe and Enhances Cisplatin Therapy in Cervical CancerCancer Res CommunMay 1, 2026Sebastian O. Wendel, Grant M. Brooke, Changkun Hu, Allison R. Sandoval, Pouya Haratipour, Long Gu, Malaney Young, Maryam Zangi, Brittany L. Rasche, N.S. Banerjee, et al.controls. For pulldown, 200 μL of protein lysate was incubated with 20 to 30 μL of preequilibrated protein A agarose beads (vortexed before use) and an appropriate dilution of PCNA rabbit primary antibody (Cell Signaling Technology,

(7) CircGRHL2 suppresses EMT and enhances sunitinib sensitivity in ccRCC via the miR-330-5p/FBXO21 axisCellular and Molecular Life SciencesApril 29, 2026Jie Gao, Huibao Yao, Qiancheng Mao, Chu Liu, Yuanshan Cui, Junjie Zhao, Jian Ma, Jitao WuTechnologies, USA) overnight at 4 °C, followed by incubation with Protein A agarose beads (Cell Signaling, #9863) for 3 h at 4 °C. Lysates were incubated with Anti-FBXO21 or Anti-p85α beads as described. Beads were

(8) Ferrodoxin 1 (FDX1) drives paclitaxel resistance in ovarian cancer via copper metabolism and ULK1/ATG13-mediated autophagy: overcome by pH/ROS-responsive PPD/PDP@si-FDX1 nanomicellesJ Exp Clin Cancer ResApril 23, 2026Yangmei Gong, Zhizhi Deng, Jie Wu, Yi HuChina), which was incubated overnight at 4 °C. After incubation, 20 µL of Protein A Agarose Beads (9863, Cell Signaling, China) was added, and the mixture was incubated for an additional 3 h. The beads were

(9) Allosteric inhibition of RAN decreases miR-126 biogenesis in endothelial cells and controls acute myeloid leukemia growthCommunications BiologyApril 14, 2026Melissa Valerio, Wenyuan Wei, Hyunjun Kang, Ning Ma, Supriyo Bhattacharya, Genevieve Baker, Weidong Hu, Lianjun Zhang, Dinh Hoa Hoang, Jia Feng, et al.per IP reaction. The next day, 50 µL of protein A/G agarose beads (Cell Signaling Technology, Cat# 9863; Cat# 37478) were added to the supernatant and incubated at 4 °C for 3 h. The beads were then washed

(10) Adropin protects against cardiac remodeling and metabolic dysfunction in a mouse model of HFpEFbioRxivApril 12, 2026Bellina A.S. Mushala, Michael W. Stoner, Maryam Sharifi-Sanjani, Janet R. Manning, Paramesha Bugga, Nisha Bhattarai, Brenda McMahon, Amber Vandevender, Steven J. Mullet, Brett A. Kaufman, et al.mouse O-GlcNAc antibody (Cell Signaling). Immunocaptured proteins were isolated using Protein-G and Protein-A agarose beads (Cell Signaling Technology, catalog number 9007), washed 5 times with 500 μL 1% CHAPS buffer, and then

(11) An EGFR co-amplified lncRNA HELDR promotes glioblastoma malignancy through KAT7-driven gene programsNature Cell BiologyMarch 27, 2026Xiaozhou Yu, Xiao Song, Richard A. Schäfer, Qingshu Meng, Deanna Tiek, Runxin Wu, Qiu He, Maya Walker, Qi Cao, Rendong Yang, et al.4 h on a rotor. The reaction mixture was subsequently incubated with protein A agarose beads (CST, 9863) at 4 °C for 1 h on a rotor. The beads were washed ten times with RIPA buffer. Proteinase K (NEB, P8107S)

(12) c-Myc transactivates CFL1 to induce senescence-like phenotype and potentiate the bystander effects for the migration and proliferation in lung cancer cellsCell Death DiscoveryMarch 26, 2026Yen-Ting Chou, Jyh-Der Leu, Wan-Yu Yang, Chien-Hsiu Li, Min-Ying Lin, Chia-Wei Kao, Yu-Chan Chang, Michael Hsiao, Yi-Jang Leeto 1000 bp, as verified by electrophoresis. The pre-cleared chromatin fragments were incubated with Protein A agarose beads and a specific anti-c-Myc antibody (Cell Signaling Technology, Danvers, MA, USA), or an anti-rabbit

(13) Investigating PKD2 deficiency-associated cardiomyopathies using hESC-cardiomyocytes and bioengineered 3D ventricular cardiac tissue stripsCell Death & DiseaseMarch 25, 2026Jingxuan Li, Wentao Peng, Maxwell Kwok, Huanyu Ding, Duan Zhuo, Bimal Gurung, Ishan Raj Lakhani, Hongyan Yu, Ellen N. Poon, Ronald A. Li, et al.HES2-CMs lysate proteins were immunoprecipitated with 2 μg of anti-PKD2 antibody or anti-PLN antibody with protein A Agarose beads (Beyotime, China) at 4 °C. Negative controls were rabbit preimmune IgG (Proteintech, USA). Immunostaining

(14) Functional rescue of a disease-linked ERAD pathway mutation via alternative splicingThe EMBO JournalMarch 20, 2026Huilun Helen Wang, Zhihong Wang, Liangguang Leo Lin, Sunil K Verma, Weronika Gniadzik, Hui Wang, Zexin Jason Li, Emily Whitestone, Lulu Jiang, Muge N Kuyumcu-Martinez, et al.(Zhou et al, 2020) or IgG (Cell Signaling Technology, 2729S) at 4 °C overnight and then followed by protein A agarose beads (Invitrogen 15918-014) for 2 h at 4 °C. Beads were washed three times with lysis buffer, boiled for

(15) De novo design of Ras isoform selective bindersCell Chem BiolMarch 19, 2026Jason Z. Zhang, Xinting Li, Alexa Rane Batingana, Caixuan Liu, Hanlun Jiang, Kevin Shannon, Benjamin J. Huang, Kejia Wu, David BakerFisher,Cat#PI26620 Spectra™ Multicolor High Range Protein Ladder,Thermo Fisher,Cat#26625 Protein A Agarose Beads,CST,Cat#9863S Protein G Agarose Beads,CST,Cat#37478 Puromycin,Tocris,Cat#4089 Hygromycin,Tocris,Cat#4137 DPBS,Gibco,Cat#14190250 Doxycycline,Tocris,Cat#4090 Studier

(16) Mycobacterium tuberculosis Rv0927c inhibits the proliferation and promotes the intrinsic apoptosis of alveolar epithelial cells through targeting mitochondrial TUFMFrontiers in ImmunologyMarch 18, 2026Aihong Xia, Xin Li, Xiang Chen, Juanjuan Quan, Jiaxu Wan, Chengkun Zheng, Zhengzhong Xu, Xinan Jiaoin western and immunoprecipitation (IP) buffer for 30 min at 4 °C. Lysates were pre-incubated with protein A agarose beads (Cell Signaling Technology) to remove non-specific adsorption, followed by overnight incubation with

(17) Gut Microbiota-Derived Propionate Governs Hepatic N2 Neutrophils in Wilson’s DiseaseCellular and Molecular Gastroenterology and HepatologyMarch 17, 2026Xiaoxiao Mi, Rongqiang Liu, Zhenyao Jiang, Mengjuan Tang, Jian Yan, Jing Liu, Yingniang Li, Jun Zheng, Wenjun Yang, Ling Gong, et al.the presence or absence of propionate (5 mM) for 24 hours. Cultured cells were lysed, pre-cleared by Protein A agarose beads, and incubated with acetylated-lysine antibody (Cell Signaling Technology) overnight at 4°C. Antigen–antibody

(18) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFront ImmunolMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar Ali#7074S) and HRP-linked anti-mouse IgG (Catalog #7076S), and protein A agarose (50% bead slurry; Catalog #9863S) were purchased from Cell Signaling Technology. Unconjugated rabbit anti-Phospho-MRGPRX2 (Ser313, Ser327/328)

(19) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFrontiers in ImmunologyMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar Ali#7074S) and HRP-linked anti-mouse IgG (Catalog #7076S), and protein A agarose (50% bead slurry; Catalog #9863S) were purchased from Cell Signaling Technology. Unconjugated rabbit anti-Phospho-MRGPRX2 (Ser313, Ser327/328)

(20) The endocytic fission protein EHD1 interacts with tubulin and regulates microtubule functionBiochimica et biophysica acta. Molecular cell researchMarch 1, 2026Ashraf B, Reddick-Umoja J, Grant J, Iyer J, Naslavsky N, Caplan S.(Sigma, #T5326), overnight at 4°C. The next day protein A agarose beads (Cell Signaling Technology, #9863S) or protein G Sepharose beads (Cytiva, #17061801) were added to the lysate-antibody mix and the negative

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Protein A Agarose Beads from Cell Signaling Technology

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Protein A Agarose Beads from Cell Signaling Technology

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