Magne HaloTag Beads, 20% Slurry from Promega

Product Specs
ItemMagne HaloTag Beads, 20% Slurry
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7281
Quantity1ml
TypeResins, Magnetic Beads
ModeAffinity Purification
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Activity-dependent ribosome profiling reveals the landscape of canonical and non-canonical translation in brain tissueNat CommunJuly 23, 2026Nayan Suryawanshi, Hitoshi Uchida, Ryo Endo, Kai Sato, Daisuke Satoh, Kazuya Tsumagari, Koshi Imami, Takayasu Mikuni, Motomasa Tanakaisolate monosomes. The monosome fraction was subjected to immunoprecipitation using HALO beads (Promega, G7282) for 15 h at 4 °C on a gentle rotor. Immunoprecipitate was washed in the lysis buffer indicated above

(2) Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responsesEMBO JJuly 3, 2026Alexis T Chang, Ricard A Rodriguez-Mias, Matthew D Berg, Sophie Moggridge, Judit Villénenzymes and other reagents","Chemicals, enzymes and other reagents" Magne® HaloTag® beads,Promega,G7282 Fe-NTA MagBeads,Cube Biotech,31501-Fe Isopropyl β-D-1-thiogalactopyranoside,Gold Bio,12481C25 benzonase

(3) Loss of FAM60A disrupts Sin3/HDAC control of the Hippo signaling and promotes oncogenic YAP1 activationCell Death & DiseaseApril 27, 2026Mojnu Miah, Md Nazmul Huda, Md Rafikul Islam, Dayebgadoh Gerald, Sneha Khator, Akash Saha, Jahangir Alam, Janet L. Thornton, Allen Gies, Michael A. Bauer, et al.Hippo “off” signals in YAP-addicted cancers. Experimental section Materials Magne® HaloTag® beads (G7281) and SNAP-Capture Magnetic Beads (S9145) were acquired from Promega (Madison, WI) and New England Biolabs

(4) Lipid droplet-induced T cell death sustains autoimmune tissue inflammationCell MetabolismApril 7, 2026Jitendra Kumar, Yoshinori Takashima, Jose Morales, Brandon Wong, Lina Werner, Isabel N. Goronzy, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jörg J. Goronzy, et al.,Cell Signaling Technology,#57058; P70380 IL-1p Rabbit pAb (A16288),Abclonal,#A16288 "Magne® HaloTag® Beads, 1mL",Promega,#G7281 NLRP3 (D2P5E) Rabbit mAb,Cell Signaling Technology,#13158; Q96P20 "PERK (EIF2AK3) Antibody (Center),

(5) Multicomplex Integrative Structural Modeling of a Human Histone Deacetylase InteractomebioRxivMarch 27, 2026Jules Nde, Kartik Majila, Rosalyn C. Zimmermann, Cassandra Kempf, Ying Zhang, Joseph Cesare, Janet L. Thornton, Jerry L. Workman, Laurence Florens, Shruthi Viswanath, et al.multifaceted role in the cell. STAR METHODS Materials Magne® HaloTag® magnetic affinity beads (G7281), Rabbit anti-HaloTag® polyclonal antibody (G9281), Sequencing Grade Modified Trypsin (V5111), Mammalian Lysis Buffer

(6) Transcriptional Regulation of Starch Biosynthesis in Sorghum Grain by a MIKC-Type MADS-Box Transcription Factor: An In Vitro AnalysisPlants (Basel)March 26, 2026Junkai Zhang, Zheyu Yan, Anqi Sun, Xiangling Gong, Hanmin Ma, Mingxi Huang, Yuxing Lin, Zhizhai Liu, Lanjie Zheng, Qianlin Xiao(Promega, Madison, WI; L3260) and subsequently affinity-purified with Magne HaloTag Beads (Promega, G7281) in accordance with the manufacturer’s instructions. The genomic DNA (gDNA) library was prepared using

(7) The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ERThe EMBO JournalMarch 25, 2026Milica Mihailovic, Aleksandra S Anisimova, Bu Erte, Ni Zhan, Ioanna Styliara, Yasin Dagdas, Gülsün Elif Karagözcentrifugation for 30 min at 4 °C. The cleared bacterial lysate was conjugated to Halo beads (Promega, G7281) overnight at 4 °C on an end-over-end rotator, washed five times with wash buffer (25 mM HEPES, pH 7.4,

(8) Circular RNA Vv‐circCOR27 modulates thermotolerance through attenuating VvHSP90 .2b‐ VvHsfA7a interaction in grapevineNew PhytologistJanuary 30, 2026Yi Ren, Yuanyuan Xu, Moyang Liu, Lipeng Zhang, Yue Song, Junpeng Li, Jingjing Liu, Dongying Fan, Zhen Zhang, Juan He, et al.Transcription/Translation System (Cat. L1170; Promega, Beijing, China) and captured onto Magne HaloTag Magnetic Beads (Cat. G7281; Promega). The beads were resuspended and incubated with the DNA library. The binding DNA segments were

(9) A bacterial translation activator with an intrinsically disordered RNA-binding regionProceedings of the National Academy of SciencesJanuary 20, 2026Pallabi Basu, Elizabeth A. Farland, James C. Charity, Kade A. Townsend, Simon L. DoveCLIP-seq. From the cell lysate, PhaF-RNA complexes were immunoprecipitated with Promega Magne HALO beads (G7282) that bind the Halo moiety of the PhaF-Halo fusion protein covalently. Following IP, beads were washed

(10) Diversification and conservation of DNA binding specificities of SPL family of transcription factorsbioRxivJanuary 16, 2026Miaomiao Li, Tao Yao, Mary Galli, Luigi Franklin Di Costanzo, Wanru Lin, Yilin Zhou, Jin-Gui Chen, Andrea Gallavotti, Shao-shan Carol HuangpIX-HALO-SPL plasmids for 3 h at 30 °C. The reaction was then mixed with 10 μl Magne HaloTag Beads (Promega, G7282) and 50 μl wash buffer (PBS + 0.05% NP40) on a rotator for 1 h at room temperature. The beads with protein

(11) High throughput mutational characterization of the GPCR ligand C5a using yeast display and deep sequencingStructureJanuary 8, 2026Yu Xu, Kaushik Thakkar, Li Guan, Yu Miao, Manal Mehibel, Robert B. Lee, David Marciano, Vignesh Viswanathan, Ziwei Wang, Jinglong Wang, et al.G6270 Promega FastBreak Cell Lysis Reagent,Promega,Cat# B8571 Promega Protease Inhibitor Cocktail,Promega,Cat# G7281 Promega HaloTEV Protease,Promega,Cat# G6601 ,, Critical commercial assays,Critical commercial assays,Critical

(12) Nucleolar MED19 regulates 2'-O-methylation of rRNA in supporting cancer cell growthNucleic Acids ResDecember 11, 2025Yang Ming, Zhao Jiamin, Peng Yu, Lu Chengjiang, Zhang Yiyang, Zhang Hanqing, Qiang Zhou, Gang Wangsupernatant of the lysate was collected after centrifugation and incubated with Magne™ HaloTag® Beads (G7281, Promega) for affinity purification. During the wash steps, the beads were washed with buffers containing

(13) Unveiling a pervasive DNA adenine methylation regulatory network in the early-diverging fungus Rhizopus microsporusNature CommunicationsNovember 21, 2025Carlos Lax, Leo A. Baumgart, Ghizlane Tahiri, Natalia Nicolás-Muñoz, Yu Zhang, Ian K. Blaby, Stephen J. Mondo, Bishoy Kamel, Ronan C. O’Malley, Vivian Ng, et al.a total volume of 100 µL, and with an incubation time of 2 h at 30 °C. Magne HaloTag Beads (Promega G7282) were washed and resuspended in binding/wash buffer (1× phosphate buffered saline + 0.005% NP40 detergent).

(14) The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylationNucleic Acids ResearchNovember 20, 2025Nana Ma, Dingkun Jiang, Tian Li, Lin Luo, Hao Chen, Xinfeng Zhang, Zongkai Mu, Shuyan Chen, Piaojuan Chen, Qing Liu, et al.3 µM ZnSO4, 100 µM EGTA, 10 mM Tris, pH 8, 0.1% Tween). Next, 2 µl of Magne HaloTag Beads (Promega, G7282) were added and incubated for 1 h at 25°C. Beads were then washed by a HydroSpeed plate washer (Tecan,

(15) The ZmFKF1b‐ZmDi19‐5 Regulatory Module Coordinates Drought Tolerance and Flowering Time in MaizePlant Biotechnology JournalOctober 8, 2025Haixia Zeng, Dandan Dou, Yang Yang, Yan Yan, Yawen Sun, Shuhan Yang, Wen Yao, Shifang Zhao, Mingle Wang, Zhixue Liu, et al.a wheat germ extract, and the recombinant protein was purified using Magen HaloTag beads (Promega, G7281). To ensure stability, 2 biological replicates were simultaneously set up during the protein purification

(16) The Diversity of the DNA ‐Binding Landscape in the DREB / ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous GenomePlant Biotechnology JournalSeptember 18, 2025Fengqi Wu, Jiakun Zheng, Huimin Hu, Hongsen Liu, Yaxuan Xiao, Junting Feng, Yanwei Hao, Chengjie Chen, Rui Xia, Zaohai ZengTNT SP6 High‐Yield Wheat Germ Protein Expression System (Promega, L3260). HaloTag magnetic beads (Promega, G7282) were used to pull down the LcERF‐Halo fusion proteins. The purified LcERF‐Halo fusion proteins were

(17) Mechanism Analysis of Transcription Factor OsERF110 Regulating Rice Pollen Response to Heavy Ion IrradiationBiologySeptember 8, 2025Sun K, Liu J, Zhang J, Li H, Zeng J, Zhou L, Guo T, Chen C.L4130). Purify and capture the expressed proteins using Magne Halo Tag Beads (Promega, Madison, WI, USA, G7281). Incubate the gDNA library linked to the OsERF110 binding beads and the adapters together. After incubation,

(18) Using DNA affinity purification sequencing (DAP-seq) to identify in vitro binding sites of potential Novosphingobium aromaticivorans DSM12444 transcription factorsMicrobiology Resource AnnouncementsAugust 14, 2025Kevin S. Myers, Alexandra M. Linz, Leo A. Baumgart, Yu Zhang, Ian Blaby, Ritesh Mewalal, Vivian Ng, Daniel R. Noguera, Timothy J. Donohuebinding/wash buffer (1× PBS, 0.1% Tween-20) containing 10 µL of pre-washed Magne HaloTag beads (Promega G7281), 1 ng of prepared genomic DNA libraries, and 1 µg of salmon sperm DNA. This mixture was incubated at

(19) Nicotinamide boosts oocyte quantity and quality by promoting N4-acetylation modification in lupus miceSci AdvJuly 18, 2025Yun Xie, Chuanchuan Zhou, Qi Guo, Yingchun Guo, Jiayi Guo, Lina Chen, Xiaoping Liu, Yanyan Zeng, Qiqi Liang, Taibao Wu, et al.was optimized as follows: Protein A coupled with HaloTag was purified using HaloTag beads (Promega, G7281). Anti-ac4Cantibody (2.5 μl; Abcam, ab252215) was incubated with 50 μl of protein A–HaloTag beads in

(20) R2HaPpY: Rapid-robust phosphotyrosine peptide enrichment using HaloTag-Src SH2 pY superbinderbioRxivMay 21, 2025Alexis Chang, Ricard A. Rodriguez-Mias, Matthew D. Berg, Sophie Moggridge, Judit Villénconcentrator,Labconco, plate and microtube centrifuge,various, bath sonicator,Branson, Magne® HaloTag® beads,Promega,G7282 Fe-NTA MagBeads,Cube Biotech,31501-Fe Isopropylβ-D-1-thiogalactopyranoside,Gold Bio,12481C25 beta-estradiol,Sigma

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