CHO-K1 from ATCC

Product Specs
ItemCHO-K1
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCCL-61
TypeCell Biology
SpeciesCricetulus griseus
TissueOvary
Cell LineCHO-K1
Validated Species ReactivityHamster (Hm)
Tissue (standardized)Ovary
ApplicationsThis cell line is suitable as a transfection host.Potential cell line for cultivated meat production

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Protocol for quantitative live-cell imaging of early GPCR trafficking using acid-stable afCFP-Venus FRETSTAR ProtocolsSeptember 1, 2026Yu Guo, Toshimasa Takahashi, Cheng Wang, Lisha Yi, Daisuke Nawa, Nanxiang Yin, Weidong Liu, Ziwei Wang, Heedong Jeon, Akitoshi Hara, et al.Cell Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines ,, CHO-K1 cell,ATCC,ATCC CCL-61 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, –,AT1R-afCFP,See “plasmid construction and

(2) Nanoscopy reveals heparan sulfate clusters as docking sites for SARS-CoV-2 attachment and entryElifeAugust 6, 2026Sue Han, Xin Wang, Tiansheng Li, Ammar Mohseni, Ivan Kosik, Chung Yu Chan, Alberto Domingo López-Muñoz, Jessica Matthias, Reid Suddaby, Zhixiong Wang, et al.(Gibco, 10082147) and incubated at 37 °C and 5% CO2 in a humidified incubator. Wild-type CHO-K1 (ATCC, #CCL-61) and GAG-deficient mutants CHO-pgsA-745 (ATCC, #CCL-2242) and CHO-pgsB-618 (ATCC, #CCL-2241) were maintained

(3) Discovery of Ribosomally Synthesized and Post-translationally Modified Knottins from the Deep-Sea Sponge Stryphnus fortisJournal of Natural ProductsJuly 24, 2026Lakmini N. Kosgahakumbura, Blazej Slazak, Norelle L. Daly, Rein Fadoul, Samia Mohamed, Erik Jacobsson, Ruisheng Xiong, Björn Hellman, Ulf Göransson, Chamari M. Hettiarachchi, et al.was also used to evaluate the cytotoxicity. Cells Chinese hamster ovary cells (CHO-K1, ATCC number CCL 61, USA) was maintained in a F-12K medium (Kaighn’s Modification of Ham’s F-12 Medium) (ATCC, USA), supplemented

(4) VEGFR2 dimerization kinetics on the cell surface determined by microscopy and mathematical modelingBiophysical JournalJuly 21, 2026Jaime Guerrero, Zachariah Malik, Fnu Bilal, Soma Jana, Aparajita Dasgupta, Khuloud Jaqamanrecommended protocol. Cell culture and transient transfections Chinese Hamster Ovary (CHO-K1, ATCC, CCL-61, Manassas, VA) cells were grown in F-12K culture medium (ATCC, 30–2004) supplemented with fetal bovine serum (FBS

(5) Comparative Real-Time Kinetics of Ligand-Receptor Interactions Using Immobilization-Based Sensing ReadoutsAnal ChemJuly 21, 2026Yazheng Wang, Yalun Wu, Lauren A. Mayse, Danny Capucilli, Aaron J. Wolfe, Liviu MovileanuGnTI cells (Thermo Fisher Scientific; catalog A39240), or CHO-K1 cells (ATCC, Manassas, VA; catalog CCL-61) using poly ethylenimine (PEI) mediated transfection. The cells were cultured in 1 L batches for 5 days

(6) Discovery of a Functional Sequon for Chondroitin Sulfate GlycosylationResearchSquareJuly 16, 2026Yoshiko Takeda-Uchimura, Yoshiko Takeda-Uchimura, Mayumi Ishihara-Aoki, Kazuchika Nishitsuji, Kazuchika Nishitsuji, Midori Mizumoto, Midori Takechi-Haraya, Eriko Takechi-Haraya, Hassan Lemjabbar-Alaoui, Fabrice Lemjabbar-Alaoui, et al.P0704S, F. meningosepticum) was purchased from New England Biolabs (Ipswich, MA); HEK293 (CRL-1573), CHO (CCL-61), and COS-7 (CRL-1651) cells were from ATCC (Manassas, VA); The mouse anti-SULF2 antibody 8G1 (MABC586)30,

(7) Predicting Clinical Sensitivities of PDGFRA Exon 18 Mutations to Imatinib and Avapritinib to Optimize Gastrointestinal Stromal Tumor TreatmentCancer Res CommunJuly 1, 2026Homma M. Khosroyani, Alina Teuber, Ajia Town, Lillian R. Klug, Denisse Evans, Jerry Call, Sara Rothschild, Neeta Somaiah, Prapassorn Thirasastr, Mengyuan Liu, et al.Table S2. Cell line authentication Parental Ba/F3 (DSMZ #ACC-300, RRID: CVCL_0161) and CHO (ATCC #CCL-61, RRID: CVCL_0214) cell lines that were used to create human PDGFRA-mutant cell lines were tested to

(8) Comparative analysis reveals molecular adaptation of mammalian HCA2 to microbial metabolitesiScienceJune 19, 2026Franziska Bischof, Petra Krumbholz, Gunnar Kleinau, Patrick Scheerer, Claudia Stäubertlines,Experimental models: Cell lines,Experimental models: Cell lines ,, CHO-K1 (passage #3 to max. #40),ATCC,CCL-61; RRID: CVCL_0214 HEK293-T (passage #3 to max #40),ATCC,CRL-3216; RRID: CVCL_0063 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, see

(9) Ultrarapid MC1R protein and associated plumage color evolution in the domestic chickenProc Natl Acad Sci U S AJune 16, 2026Cheng Ma, Aenne-Dorothea Liebing, Gunnar Kleinau, Antje Kamprad, Louisa Calabrese, Michal Szczepek, Zheng Li, Mårten Larsson, Patrick Scheerer, Claudia Stäubert, et al.carried out as previously described (58). In short, the Chinese hamster ovary cell line CHO-K1 (ATCC CCL-61) was obtained from the American Type Culture Collection and maintained at 37 °C in a humidified 5% CO2

(10) Immunotherapeutic and Antitumoral Potential of Fatty Acids from Subantarctic Macroalgae (Sarcopeltis skottsbergii)Mar Biotechnol (NY)June 6, 2026Gilca Costa Nachtigal, Bruna Silveira Pacheco, Fernanda Severo Sabedra Sousa, Maria Eduarda Ehlert, Tallyson Nogueira Barbosa, Nicole Ramos Scholl, Cristina Jansen Alves, Claudio Martin Pereira de Pereira, Andrés Mansilla, Sibele Borsuk, et al.HCC1954 (BCRJ: 0280; ATCC: CRL-2338) (HER-2 + breast cancer), non-tumoral CHO-K1 (BCRJ: 0069; ATCC; CCL-61) and macrophage J774A.1 (BCRJ: 0121; ATCC; TIB-67), all obtained from Rio de Janeiro Cell Bank (PABCAM,

(11) Recombinant LGR4 extracellular domain attenuates osteoclastogenesis and prevents bone metastasis via RANKL inhibitionJournal of Bone OncologyMay 19, 2026Meng Fu, Guowei Chang, Shuilin Shao, Liying Zhaoto confirm insert presence. 2.2 Cell culture and stable cell line establishment CHO-K1 cells (ATCC CCL-61) were maintained in CD CHO medium (Gibco, 10743029) under suspension culture conditions using polycarbonate

(12) Cannabinoid CB2 receptor drives trastuzumab resistance and predicts durable anti-HER2 responseOncogeneMay 11, 2026Marta Seijo-Vila, Sofía A. Balsinde, Sandra Blasco-Benito, Isabel Tundidor, María Rubert-Hernández, Ana Montero-Calle, Rodrigo Barderas, Laura E. Kilpatrick, Simon Platt, Noemi Karsai, et al.de Recerca Contra la Leucèmia Josep Carreras. Barcelona, Spain) and are described in [13]. CHO-K1 (#CCL-61, RRID:CVCL_0214) and HEK293T cells (#CRL-3216, RRID:CVCL_0063) (both female) were obtained from EACR

(13) Nanoscale organization in the cell membrane dynamically modulates the biophysics of voltage-gated sodium channelsNature CommunicationsMay 8, 2026Mikhail Tarasov, Madison Ammon, Jan Otto Wirth, Christopher Hampton, Zoja Selimi, Rengasayee Veeraraghavan, Przemysław B. Radwańskicultured at 37 °C in 5% CO2 in a humidified atmosphere. A blank CHO cell line (CHO-K1, ATCC cat. # CCL-61) was cultured under the same conditions but without antibiotics. Mouse cDNA for WT SCN5A (NCBI Nucleotide

(14) A molecular stabiliser of an inhibitory eIF2B-eIF2(αP) complex activates the Integrated Stress ResponseNature CommunicationsMay 6, 2026Fiona Shilliday, Miguel Gancedo-Rodrigo, Ginto George, Shintaro Aibara, Santosh Adhikari, Syedah Neha Ashraf, Evelyne J. Barrey, Paolo A. Centrella, Damian Crowther, Paige Dickson, et al.were used in the experiments described in Fig. 4. Adherent Chinese Hamster Ovary (CHO-K1) cells (ATCC CCL-61) were cultured in Ham’s F12 nutrient mixture (Sigma), supplemented with 10% (v/v) serum (FetalClone-2,

(15) Engineered NLS-PBase system boosts stability and productivity in recombinant cell linesBioresour BioprocessApril 28, 2026Yanan Zhou, Yuan Tian, Qingyuan Ran, Qian Ye, Wen-Song Tanscreening. Materials and methods Cell line and cell culture The adherent CHO-K1 cell line (ATCC, Virginia, USA; No. CCL-61) used in this work was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Bioengine, Shanghai, China)

(16) Structural basis for the differential recognition of integrin αvβ3 by rhodostomin and trimucrinCommunications biologyApril 27, 2026Wang YT, Chang YT, Huang CH, Liau CT, Chen CY, Chuang WJ.integrins αvβ3 were kindly provided by Dr. Y. Takada at Scripps Research Institute, derived from ATCC CCL-61; CHO-K1 cells) were mixed with Rho, Tmu, their mutants at five different concentrations, or PBS for

(17) Metabolite import by SLC33A1 is required for ATF6 activation during endoplasmic reticulum stressLife Sci AllianceApril 17, 2026Ginto George, Heather P Harding, Richard Kay, David Ron, Adriana OrdoñezKey Resources Tables. Mammalian cell culture Adherent Chinese hamster ovary (CHO-K1) cells (ATCC CCL-61) were cultured in Ham’s F12 nutrient mixture (Sigma-Aldrich), supplemented with 10% (vol/vol) serum

(18) Ghosts on the Membrane: Cytoskeletal Pinning Influences Nanoscale Cell Membrane OrganizationBiomoleculesApril 17, 2026Shambhavi Pandey, Thorsten Wohlandspatial organization and dynamics of the PM. 2. Materials and Methods 2.1. Cell Culture CHO-K1 cells (CCL-61™, ATCC; Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose with

(19) A series of spontaneously blinking dyes for super-resolution microscopyNature MethodsApril 15, 2026Katie L. Holland, Sarah E. Plutkis, Brian P. English, Timothy A. Daugird, Abhishek Sau, Jonathan B. Grimm, Qinsi Zheng, Ankith Sharma, Sandeep Dave, Anja Schmidt, et al.(HTB-96), hTERT-immortalized retinal pigment epithelial cells (hTERT RPE-1; CRL-4000) and CHOK1 cells (CCL-61) were obtained from the ATCC. For imaging of cell nuclei using the HaloTag system, COS-7 cells with

(20) Structural insights into single-pass transmembrane receptor GC-A activation by distinct antihypertensive antibodiesNature CommunicationsApril 6, 2026Shian Liu, Onorina Manzo, Jinan Wang, Lan Zhu, Fu Xiao, Yi-Chen Su, Devanshu Kurre, Wei Liu, Yinglong Miao, Annarita Di Lorenzo, et al.vs. response function of Prism 10 (GraphPad)20,21,34. Ligand binding experiment CHO-K1 cells (ATCC CCL-61) were cultured in F-12K medium containing 10% fetal bovine serum and transfected with wild-type full-length

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CHO-K1 from ATCC

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