(–)-Blebbistatin from Cayman Chemical

Product Specs
Item(–)-Blebbistatin
CompanyCayman Chemical
Price $269.00Supplier Page View Company Product Page
Catalog Number13013
Research AreaApoptosis, Cell Biology, Cell Death, Cytoskeleton & Motor Proteins
FormatA crystalline solid
Molecular Weight292.3
Purity≥98%
Storage-20
FormulaC18H16N2O2
OriginUSA
TypeInhibitors
Quantity5 mg

Specifications/Features

Ki, IC, EC, or working concentration: IC50: 0.5-5.0 µM for Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB
IC50: 80 µM for smooth muscle myosin

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Subcellular proteomic analyses reveal REEP5 knockdown in the mouse heart disrupts mitochondrial networksMolecular & Cellular ProteomicsFebruary 9, 2026Michelle Di Paola, Cristine J. Reitz, Uros Kuzmanov, Kateleen Jia, Anthony O. GramoliniWorthington Biochemical Corporation, #LS004176) buffer containing blebbistatin (Cayman Chemical Corporation, #13013). The cell suspension was passed through a 70 μm filter and subjected to sequential gravity sedimentation

(2) Bioenergetic responses to β-adrenergic stimulation in beige adipocyte depend on actomyosin driven forcesbioRxivDecember 1, 2025Yuchen He, Lu Ling, Garrett Dempsey, Nayiri Kalindjian, Zoie Verghese, Erzhen Chen, Irene Liparulo, Isabel Cho, Vyvylyn Tran, Sanjay Kumar, et al.differentiation day 7. Vendors and concentrations of compounds are listed below: Blebbistatin (50 mM, Cayman, #13013), EMD-57003 (10 mM, Sigma-Aldrich, #530657), PF-573,228 (10 mM, Sigma-Aldrich, #PZ0117), isoproterenol

(3) Cell-intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrowProceedings of the National Academy of SciencesJuly 16, 2024Roberto Alonso-Matilla, Alice R. Lam, Teemu P. Miettinen1:1,000 dilution (v/v) of EtOH (vehicle for Brefeldin A), 50 µM (-)-Blebbistatin (Cayman Chemical, #13013) for 2 h, 2 µM Latrunculin B (Cayman Chemical, #10010631) for 2 h, 10 µM Brefeldin A (ThermoFisher,

(4) Differential cellular stiffness across tissues that contribute to Xenopus neural tube closureDevelopment, Growth & DifferentiationJune 26, 2024Makoto Suzuki, Naoko Yasue, Naoto Uenomicro‐dissecting forceps. To reduce actomyosin activity levels, embryos were treated with 50 μM blebbistatin (#13013; Cayman) for 3–4 h or 0.5 μM latrunculin B (BML‐T110; Enzo) for 10 min before being embedded. To measure

(5) Myosin-independent stiffness sensing by fibroblasts is regulated by the viscoelasticity of flowing actinCommunications MaterialsMay 14, 2024Nikhil Mittal, Etienne B. Michels, Andrew E. Massey, Yunxiu Qiu, Shaina P. Royer-Weeden, Bryan R. Smith, Alexander X. Cartagena-Rivera, Sangyoon J. HanGrand Island, NY, USA; 10131035) in 5% CO2 at 37 °C. To inhibit myosin-II activity, (−)-Blebbistatin (13013) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Formin activity inhibitor SMIFH2 (340316-62-3)

(6) N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clustersElifeDecember 12, 2023Anshuman Bhanja, Margaret K. Seeley-Fallen, Michelle Lazzaro, Arpita Upadhyaya, Wenxia Songcompound,Wiskostatin,Millipore Sigma,W2270-5MG,N-WASP inhibitor (10 µM) Chemical compound,Blebbistatin,Cayman Chemicals,13013,NMII inhibitor (50 µM) Antibody,Rabbit IgG anti-mouse Arp2 antibody (rabbit polyclonal),Abcam,ab47654,IF

(7) Cell intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrowbioRxivNovember 29, 2023Roberto Alonso-Matilla, Alice Lam, Teemu P. Miettinenchemical treatments of live cells, we used DMSO (vehicle), 50 µM (–)-Blebbistatin (Cayman Chemical, Item #13013) or 2 µM Latrunculin B (Cayman Chemical, Item #10010631) for 2 hours. For live cells experiments, the

(8) Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletionBMC biologyFebruary 1, 2023Stark M, Levin M, Ulitsky I, Assaraf YG.Y27632 (#sc-281642), and CyMl (#sc-500804) were from Santa Cruz Biotechnology, Dallas, TX, USA. Blebb (#13013) was from Cayman Chemical, Ann Arbor, MI, USA. FA (#J62937) was from Alfa Aesar, Tewksbury, MA, USA. Tissue culture

(9) Adenylyl cyclase isoform 1 contributes to sinoatrial node automaticity via functional microdomainsJCI insightNovember 22, 2022Ren L, Thai PN, Gopireddy RR, Timofeyev V, Ledford HA, Woltz RL, Park S, Puglisi JL, Moreno CM, Santana LF, et al.indicated otherwise. Laminin (catalog 23017015) was obtained from Invitrogen, blebbistatin (catalog 13013) was obtained from Cayman Chemical, and IBMX (catalog 2845) was obtained from Tocris Bioscience. Data

(10) ROCK1 mechano-signaling dependency of human malignancies driven by TEAD/YAP activationNature communicationsFebruary 4, 2022Esposito D, Pant I, Shen Y, Qiao RF, Yang X, Bai Y, Jin J, Poulikakos PI, Aaronson SA.chemicals were solubilized in DMSO. Latrunculin B (Sigma, L5288) and Blebbistatin (Cayman Chemical, 13013). ROCK inhibitors were as follows: SAR407899 (Cayman Chemical, 21717) Y-27632 (Tocris Bioscience, 1254),

(11) Deciphering cellular signals in adult mouse sinoatrial node cellsiScienceJanuary 21, 2022Reddy GR, Ren L, Thai PN, Caldwell JL, Zaccolo M, Bossuyt J, Ripplinger CM, Xiang YK, Nieves-Cintrón M, Chiamvimonvat N, et al.M1018 "2,3-Butanedione 2-monoxime (BDM)",Sigma Aldrich,Cat# B0753; CAS: 57-71-6 Blebbistatin,Cayman,Cat# 13013; CAS: 856925-71-8 (S)-nitroblebbistatin,Cayman,Cat# 13891; CAS: 856925-75-2 Forskolin,Sigma-Aldrich,Cat#

(12) The effective use of blebbistatin to study the action potential of cardiac pacemaker cells of zebrafish (Danio rerio) during incremental warmingCurrent Research in PhysiologyJanuary 17, 2022L. Marchant James, M. Smith Frank, P. Farrell Anthonysolution was then replaced with fresh saline containing 10 μM blebbistatin (Cayman Chemicals catalog #13013, Burlington, ON, Canada). Blebbistatin was dissolved in DMSO (10 mM stock solution) and stored at −20°C

(13) Condensation tendency and planar isotropic actin gradient induce radial alignment in confined monolayerseLifeSeptember 20, 2021Xie T, St Pierre SR, Olaranont N, Brown LE, Wu M, Sun Y.cell nucleus. Small-molecule drugs treatment assays Blebbistatin (10 µM, Cayman Chemical, cat.no.13013), Y27632 (10 µM, Cayman Chemical, cat.no.10005583), and Aphidicolin (1 µg/ml, Cayman Chemical, cat.no.14007)

(14) Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3DAdvanced healthcare materialsJune 1, 2021Berg IC, Mohagheghian E, Habing K, Wang N, Underhill GH.manufacturers: EGF (PeproTech, AF-100-15) 50 ng ml−1 in PBS with 1% w/v BSA; (−)-blebbistatin (Cayman Chemical, 13013), 1 mg ml−1 in dimethyl sulfoxide (DMSO); Y-27632 (Enzo Life Sciences, 270-333-M005), 5 mg ml−1 in deionized

(15) A simple and robust cell-based assay for the discovery of novel cytokinesis inhibitorsJournal of Biological MethodsSeptember 17, 2020Laszlo Radnai, Rebecca F. Stremel, Thomas Vaissiere, Li Lin, Michael Cameron, William H. Martin, Gavin Rumbaugh, Theodore M. Kamenecka, Patrick R. Griffin, Courtney A. MillerArbor, MI)✓ Swinholide A (Cat. # 501146229, Fisher Scientific, Hanover Park, IL)✓ Blebbistatin (Cat. # 13013, Cayman Chemical, Ann Arbor, MI)✓ para-aminoblebbistatin (Cat. # 22699, Cayman Chemical, Ann Arbor,

(16) Matrix mechanics and water permeation regulate extracellular vesicle transportNature nanotechnologyMarch 1, 2020Lenzini S, Bargi R, Chung G, Shin JW.polystyrene plates with retention medium. If applicable, hydrogels were treated with blebbistatin (Cayman 13013) or Y-27632 (Cayman 10005583). If necessary, gels were digested by adding medium with 3.4 mg/mL alginate

(17) Myosin IIA and formin dependent mechanosensitivity of filopodia adhesionNature communicationsAugust 9, 2019Alieva NO, Efremov AK, Hu S, Oh D, Chen Z, Natarajan M, Ong HT, Jégou A, Romet-Lemonne G, Groves JT, et al.or 50 μM Rho-kinase (ROCK) inhibitor Y-27632 (Y0503, Sigma-Aldrich), or 20 μM S-nitro-blebbistatin (13013-10, Cayman Chemicals) was added for 10–20 min before the experiments or directly during observation.

(18) Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signalingeLifeDecember 27, 2018Kaylan KB, Berg IC, Biehl MJ, Brougham-Cook A, Jain I, Jamil SM, Sargeant LH, Cornell NJ, Raetzman LT, Underhill GH.Factor or drug,Stock,Target,Manufacturer,Catalog # (–)-Blebbistatin,1 mg/ml,25 μM,Cayman Chemical,13013 DECMA,1 mg/ml,10 μg/ml,Fisher Scientific,50-245-625 FR180204,10 mg/ml,10 μM,Sigma-Aldrich,SML0320 "L-685,458

(19) Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epitheliaeLifeJune 13, 2018McKinley KL, Stuurman N, Royer LA, Schartner C, Castillo-Azofeifa D, Delling M, Klein OD, Vale RD.S -- Blebbistatin,Myosin II inhibitor,Abcam,ab120491,200 µM S -- Blebbistatin,Myosin II inhibitor,Cayman,13013,200 µM Y27632,ROCK inhibitor,Selleck,S1049,10 µM DAPT,Gamma-secretase (Notch) inhibitor,Abcam,ab120633,50

(20) Emergent patterns of collective cell migration under tubular confinementNature CommunicationsDecember 1, 2017Wang Xi, Surabhi Sonam, Thuan Beng Saw, Benoit Ladoux, Chwee Teck Limmyosin-II inhibition, cells were treated with 6, 12.5, 25, 50, and 100 μM blebbistatin (Cayman Chemical, 13013) 30 min before imaging. Image analysis Virtual opening of the TCSs was performed after background

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(–)-Blebbistatin from Cayman Chemical

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Specifications/Features

Cayman Chemical

Citations (22)

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(–)-Blebbistatin from Cayman Chemical

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