GW 4869 (hydrochloride hydrate) from Cayman Chemical

Product Specs
ItemGW 4869 (hydrochloride hydrate)
CompanyCayman Chemical
Price $39.00Supplier Page View Company Product Page
Catalog Number13127
Research AreaImmunology & Inflammation
FormatA crystalline solid
Molecular Weight577.5
Purity≥90%
Storage-20
FormulaC30H28N6O2 • 2HCl [XH2O]
OriginUSA
TypeInhibitors
Quantity500 µg

Specifications/Features

Ki, IC, EC, or working concentration: IC50: 1 µM

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Unconventional Secretion of Angiogenic Sonic Hedgehog–Containing Extra‐Large Extracellular Vesicles is Driven by PI3K–Rab18‐GDP SignallingJournal of Extracellular BiologyFebruary 1, 2026Shuo Wang, Rio Imai, Yuya Kaneko, Yosuke Tanaka(MedChemExpress, HY‐15785) was dissolved at 1 mM in DMSO and used at 0.5 µM for 48 h. GW4869 (Cayman Chemical, #13127) was dissolved at 5 mM in DMSO and used at 1.25 µM (typically overnight). SF1670 (Selleck, S7310) was

(2) Extracellular Vesicle Inhibitors Enhance Cholix-Induced Cell Death via Regulation of the JNK-Dependent PathwayToxinsAugust 29, 2024Ozaki K, Nagahara H, Kawamura A, Ohgita T, Higashi S, Ogura K, Tsutsuki H, Iyoda S, Yokotani A, Yamaji T, et al.anti-mouse (#HAF007) secondary antibodies were purchased from R&D Systems (Minneapolis, MN, USA). GW4869 (#13127), altenusin (#26911), DMA (#19100), imipramine (#15890), and SP600125 (#10010466) were purchased from

(3) Ceramide‐mediated orchestration of oxidative stress response through filopodia‐derived small extracellular vesiclesJournal of Extracellular VesiclesJuly 11, 2024Zainuddin Quadri, Ahmed Elsherbini, Simone M. Crivelli, Salim S. El‐Amouri, Priyanka Tripathi, Zhihui Zhu, Xiaojia Ren, Liping Zhang, Stefka D. Spassieva, Mariana Nikolova‐Karakashian, et al.,Cayman Chemical Company,13583 Cayman Chemical Company,13583,Acid (Arc39) GW4869 (Hydrochloride hydrate),Cayman Chemical Company,13127

(4) Host extracellular vesicles confer cytosolic access to systemic LPS licensing non-canonical inflammasome sensing and pyroptosisNature cell biologyDecember 1, 2023Kumari P, Vasudevan SO, Russo AJ, Wright SS, Fraile-Ágreda V, Krajewski D, Jellison ER, Rubio I, Bauer M, Shimoyama A, et al.some experiments, Casp11−/− mice were injected with DMSO or EV inhibitor (2.5 μg/g GW4869 [catalog no. 13127; Cayman chemical]) dissolved in Trappsol (catalog no. THPB-P; CDT Inc) on days 1 and 2, and then injected

(5) Sphingomyelin 16:0 is a therapeutic target for neuronal death in acid sphingomyelinase deficiencyCell death & diseaseApril 6, 2023Gaudioso Á, Jiang X, Casas J, Schuchman EH, Ledesma MD.Sphingomyelin (Merck; #860600 P; 40 µM); Desipramine (Merck; # D3900; 10 µM) and GW4869 (Cayman Chemicals; #13127; 15 µM) were added, from stocks dissolved in 100% ethanol, to cultured neurons 48 h before processing. Quantification

(6) Renomedullary exosomes produce antihypertensive effects in reversible two-kidney one-clip renovascular hypertensive miceBiochemical pharmacologyOctober 1, 2022Hu G, Li G, Huang D, Zou Y, Yuan X, Ritter JK, Li N, Li PL.exosome-modulating drugs, rapamycin (6 mg/kg BW) [R0395, Sigma-Aldrich, St. Louis, MO, USA] or GW4869 (1 mg/kg BW) [13127, Cayman, Ann Arbor, MI, USA], injected intraperitoneally once every other day until sacrifice. Rapamycin

(7) The identification of novel small extracellular vesicle (sEV) production modulators using luciferase‐based sEV quantification methodJournal of Extracellular BiologySeptember 27, 2022Aki Yamamoto, Yuki Takahashi, Shinsuke Inuki, Shumpei Nakagawa, Kodai Nakao, Hiroaki Ohno, Masao Doi, Yoshinobu Takakuracells/well). After 24 h of incubation, the cells were treated with or without 5 mg/ml GW4869 (item no. 13127; Cayman Chemical, Ann Arbor, MI, USA) suspended in Opti‐MEM (Thermo Fisher Scientific) and incubated

(8) Epstein-Barr virus tegument protein BGLF2 in exosomes released from virus-producing cells facilitates de novo infectionCell communication and signaling : CCSJune 21, 2022Sato Y, Yaguchi M, Okuno Y, Ishimaru H, Sagou K, Ozaki S, Suzuki T, Inagaki T, Umeda M, Watanabe T, et al.(Piscataway, NJ, USA). For inhibition of exosome secretion, cells were treated with 10 μM GW4869 (Cat# 13127; Cayman Chemical, Ann Arbor, MI, USA). Transient transfection of poly(I:C) (Cat# 4287/10; R&D Systems,

(9) LGALS3 (galectin 3) mediates an unconventional secretion of SNCA/α-synuclein in response to lysosomal membrane damage by the autophagic-lysosomal pathway in human midbrain dopamine neuronsAutophagyMay 1, 2022Burbidge K, Rademacher DJ, Mattick J, Zack S, Grillini A, Bousset L, Kwon O, Kubicki K, Simon A, Melki R, et al.InvivoGen, tlrl-wtm), TD139 (1 µM; MedChem Express, HY-19940), GW4869 (10 µM; Cayman Chemical Company, 13127), and LLOME (2 µM; Santa Cruz Biotechology, sc-285992A). All experiments involving drug treatments,

(10) The Pathogenic Sphingolipid Psychosine is Secreted in Extracellular Vesicles in the Brain of a Mouse Model of Krabbe DiseaseASN NeuroMarch 18, 2022Cory R. Reiter et al.pharmacologic inhibition of neutral sphingomyelinase 2 (nSMase2) by GW4869 (Cayman Chemical, RRDI: 13127) (2.5mg/kg) or DMSO vehicle only controls, starting at postnatal day 10 (P10) with separate groups for

(11) Extracellular vesicles synchronize cellular phenotypes of differentiating cellsJournal of extracellular vesiclesSeptember 1, 2021Minakawa T, Matoba T, Ishidate F, Fujiwara TK, Takehana S, Tabata Y, Yamashita JK.inhibit the secretion of EVs, aggregates were treated with manumycin A (BVT‐0091, BioViotica) or GW4869 (13127, Cayman Chemical). 2.5 Aggregate formation ESC lines were seeded on a low adhesion dish, PrimeSurface

(12) Pro-inflammatory β cell small extracellular vesicles induce β cell failure through activation of the CXCL10/CXCR3 axis in diabetesCell reportsAugust 24, 2021Javeed N, Her TK, Brown MR, Vanderboom P, Rakshit K, Egan AM, Vella A, Lanza I, Matveyenko AV.C5789 AMG487,MedChemExpress,Cat# HY-15319 Complete macrophage medium w/kit,CellBiologics,Cat# M3368 GW4869,Cayman Chemical,Cat# 13127 Critical commercial assays,Critical commercial assays,Critical commercial assays ExoGlow-membrane

(13) Microglia modulate stable wakefulness via the thalamic reticular nucleus in miceNature communicationsJuly 30, 2021Liu H, Wang X, Chen L, Chen L, Tsirka SE, Ge S, Xiong Q.glycol, and 70% saline without carmofur served as the vehicle. The SMase2 inhibitor GW-4869 (Item No. 13127, Cayman) was dissolved in DMSO (5 mg/ml) and then diluted with saline for i.p. injection (0.625 mg/kg)

(14) Age-related defects in autophagy alter the secretion of paracrine factors from bone marrow mononuclear cellsAgingJune 4, 2021Yeganeh A, Alibhai FJ, Tobin SW, Lim F, Wu J, Li S, Weisel RD, Li RK.hours, and 10 mM concentration for 3-4 hours, respectively. EV secretion was inhibited using GW4869 (#13127, Cayman Chemical) at 20 μM, for 24 hours. TGF-β1 Ab (1 μg/mL) (#MAB240, R&D Systems) was used to neutralize

(15) Generation and Application of a Reporter Cell Line for the Quantitative Screen of Extracellular Vesicle ReleaseFrontiers in pharmacologyJanuary 1, 2021Shpigelman J, Lao FS, Yao S, Li C, Saito T, Sato-Kaneko F, Nolan JP, Shukla NM, Pu M, Messer K, et al.InvivoGen), 50 ng/ml or graded concentrations of PMA, 5 µM compound 5, 5 µM compound 81, 5 µM GW4869 (GW, 13127, Cayman Chemical Company, Ann Arbor, MI, United States) or 1 µM manumycin A (MA, 10010497, Cayman Chemical Company

(16) Programmable Extracellular Vesicles for Macromolecule Delivery and Genome ModificationsDevelopmental cellDecember 21, 2020Zhang X, Xu Q, Zi Z, Liu Z, Wan C, Crisman L, Shen J, Liu X.Carbonyl cyanide m-chlorophenyl hydrazone",Sigma-Aldrich,CAS#555-60-2 GW4869,Cayman Chemical,CAT# 13127 Recombinant (E. Coli) rGFP protein,PROSPEC,CAT#:pro-687-a Protein-G Magnetic beads,Thermo Scientific,Catalog#.:

(17) The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesiclesNature cell biologyFebruary 1, 2020Leidal AM, Huang HH, Marsh T, Solvik T, Zhang D, Ye J, Kai F, Goldsmith J, Liu JY, Huang YH, et al.Bafilomycin A1 (Sigma, B1793) as indicated for 1 h prior to lysis. Treatment with 5μM GW4869 (Cayman, 13127) or vehicle (DMSO, Sigma) in serum free DMEM for 24h was used to inhibit nSMase activity. Plasmid constructs The

(18) Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signaturesNature communicationsJanuary 18, 2018Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.cycloheximide (50 µg/ml, C4859, Sigma-Aldrich), EGF (200 ng/ml, 01–101, EMD Millipore), GW4869 (10 µM, 13127, Cayman Chemicals), SAR405 (1 µM, HY-12481, MedChem Express), VPS34IN1 (1 or 3 µM, Dundee University),

(19) MEX3C interacts with adaptor-related protein complex 2 and involves in miR-451a exosomal sortingPloS oneJanuary 1, 2017Lu P, Li H, Li N, Singh RN, Bishop CE, Chen X, Lu B.sphingomyelinase 2 inhibitor GW 4869 (hydrochloride hydrate) was purchased from Cayman Chemical Co. (Item no. 13127). Puromycin and Bafilomycin A1 were purchased from Sigma. Plasmids Plasmid DNA constructs for the

(20) KRAS-dependent sorting of miRNA to exosomeseLifeJuly 1, 2015Diana J Cha, Jeffrey L Franklin, Yongchao Dou, Qi Liu, James N Higginbotham, Michelle Demory Beckler, Alissa M Weaver, Kasey Vickers, Nirpesh Prasad, Shawn Levy, et al.with PBS and cultured for 24 hr in serum-free medium containing either 5 μM GW4869 (Cayman Chemicals # 13127, Ann Arbor, MI, USA) or DMSO. Medium was removed and cells were washed three times with PBS and fixed

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GW 4869 (hydrochloride hydrate) from Cayman Chemical

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Specifications/Features

Cayman Chemical

Citations (21)

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GW 4869 (hydrochloride hydrate) from Cayman Chemical

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