H-89 (hydrochloride) from Cayman Chemical

Product Specs
ItemH-89 (hydrochloride)
CompanyCayman Chemical
Price $238.00Supplier Page View Company Product Page
Catalog Number10010556
Research AreacAMP Signaling, Cell Biology, Cell Signaling
FormatA crystalline solid
Molecular Weight519.3
Purity≥98%
Storage-20
FormulaC20H20BrN3O2S • 2HCl
OriginCHN
TypeInhibitors
Quantity25 mg

Specifications/Features

Ki, IC, EC, or working concentration: IC50: 0.14 µM for PKA
Ki: 48 nM for PKA
IC50: 0.08 µM for S6K1
IC50: 0.12 µM for MSK1
IC50: 0.27 µM for ROCK-II
IC50: 2.6 µM for PKBalpha
IC50: 2.8 µM for MAPKAP-K1b

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammationCell ReportsJanuary 27, 2026Jun Nagai, Madeline M. Hastings, Airi Nishida, Sreyashi Majumdar, Kendall Zaleski, Sofia A. Marshall, Junrui Lin, Mira A.S. Saliman, Yumi Tani, Yamato Murakami, et al.,Cayman Chemical,20310 Leukotriene E4,Cayman Chemical,20410 Forskolin,Cayman Chemical,11018 H-89 (hydrochloride),Cayman Chemical,10010556 PF-04418948,Cayman Chemical,15016 EP4 Receptor Antagonist 1,Cayman Chemical,32722 Dimethyl sulfoxide,Sigma-Aldrich,D2650-5X10ML Cicaprost,SantaCruz,sc-358799A Bucladesine (sodium salt

(2) GPCR endocytosis rewires neuronal gene expression and cellular architecturebioRxivSeptember 1, 2025Katherine L. Hall, Matthew J. Klauer, Nikoleta G. Tsvetanovaresuspended to 100 μM in DMSO and used at a final concentration of 100 nM. H89 (Cayman Chemical, Cat #10010556) was dissolved in DMSO to 10 mM and used at 10 μM final concentration. 8-bromo-cAMP (Abcam, #ab141448)

(3) Deletion of EP3 prostaglandin receptor in murine macrophages aggravates diet-induced obesity by suppressing SPARCThe EMBO JournalJuly 23, 2025Wenlong Shang, Yinxiu Li, Lu Wang, Jiao Liu, Huiwen Ren, Qian Liu, Shumin Guo, Yuhong Wang, Yubo Ma, Tianyi You, et al.rosiglitazone (#D2408), and d-glucose (#G6152) were purchased from Sigma-Aldrich (St. Louis, MO, USA). H-89 (#10010556) and Mithramycin A (#11434) were purchased from the Cayman Chemical Company (Ann Arbor, MI, USA). Palmitate

(4) Phosphatases control the duration and range of cAMP/PKA microdomainsFunction (Oxf)March 24, 2025Filippo Conca, Doruk Kaan Bayburtlu, Mauro Vismara, Nicoletta C Surdo, Alessandra Tavoni, Leonardo Nogara, Adamo Sarra, Stefano Ciciliot, Giulietta Di Di Benedetto, Liliana F Iannucci, et al.11018), 3-isobutyl-1-methylxantine (IBMX, Cat. No. 13347), and H-89 dihydrochloride (H-89, Cat. No. 10010556) were purchased from Cayman Chemical (USA). Dimethyl sulfoxide (DMSO, Cat. No. D8418), phosphate-buffered

(5) A conserved transcription factor regulatory program promotes tendon fateDevelopmental CellDecember 1, 2024Xubo Niu, Delmy L. Melendez, Suyash Raj, Junming Cai, Dulanjalee Senadeera, Joseph Mandelbaum, Ilya A. Shestopalov, Scott D. Martin, Leonard I. Zon, Thorsten M. Schlaeger, et al.,Cayman Chemical,11018 Colforsin,Cayman Chemical,11214 8-Bromo-cAMP,Sigma,23583–48-4 IBMX,Cayman Chemical,13347 H-89,Cayman Chemical,10010556 ESI-09,Cayman Chemical,19130 L-cis-Diltiazem HCl,Enzo,BML-CA207–0005 66615,Tocris,5661 CBP112,Cayman Chemical

(6) Extensive location bias of the GPCR-dependent translatome via site-selective activation of mTORbioRxivJune 23, 2024Matthew J Klauer, Caitlin AD Jagla, Nikoleta G Tsvetanovaresuspended in 10 mM HEPES buffer and used at 150 μg/mL final concentration. H89 (Cayman Chemical, Cat #10010556) was dissolved in DMSO to 10 mM and used at 10 μM final concentration. RPS6 mouse monoclonal antibody

(7) Effects of Akt Activator SC79 on Human M0 Macrophage Phagocytosis and Cytokine ProductionCellsMay 24, 2024Lee RJ, Adappa ND, Palmer JN.(13311) GSK690693,Akt inhibitor,Cayman Chemical (16891) H89,Protein kinase A (PKA) inhibitor,Cayman Chemical (10010556) KH 7,Soluble adenylyl cyclase (sAC) inhibitor,Cayman Chemical (13243) L-NAME,Nitric oxide synthase

(8) Molecular determinants and signaling effects of PKA RIα phase separationMolecular CellApril 18, 2024Julia C. Hardy, Emily H. Pool, Jessica G.H. Bruystens, Xin Zhou, Qingrong Li, Daojia R. Zhou, Max Palay, Gerald Tan, Lisa Chen, Jaclyn L.C. Choi, et al.,Sigma Aldrich,Cat#I7018 Isoproterenol,Sigma Aldrich,Cat#1351005 Propranolol,Sigma,Cat#P0884 H89,Cayman Chemical,Cat#10010556 Rapamycin,LC Labs,Cat#R-5000 CTZ400a (DeepBlueC),Nanolight Technology,Cat#340 Noble Agar,ThermoFisher,Cat#

(9) Endosome positioning coordinates spatially selective GPCR signalingNature Chemical BiologyFebruary 1, 2024Blair K. A. Willette, Jin-Fan Zhang, Jin Zhang, Nikoleta G. TsvetanovaCalbiochem (Cat # 344281) and used at 50μM final concentration. H89 was purchased from Cayman Chemical (Cat #10010556) and used at 20μM final concentration. Construct cloning A previously described lentiviral plasmid

(10) Neuronal ER-plasma membrane junctions couple excitation to Ca2+-activated PKA signalingNat CommunAugust 26, 2023Nicholas C. Vierra, Luisa Ribeiro-Silva, Michael Kirmiz, Deborah van der List, Pradeep Bhandari, Olivia A. Mack, James Carroll, Elodie Le Monnier, Sue A. Aicher, Ryuichi Shigemoto, et al.solution immediately prior to stimulation of neurons. In some experiments, H89 (Cayman Chemical Cat# 10010556) or nimodipine (Alomone Cat# N-150) were included at the indicated concentrations. Hexanediol and forskolin/IBMX

(11) Mechanism of cis-Nerolidol-Induced Bladder Carcinoma Cell DeathCancersFebruary 3, 2023Glumac M, Čikeš Čulić V, Marinović-Terzić I, Radan M.molecular inhibitors: xestospongin C (XSC; 64950), dandrolene-Na (DRN; 14326), SB203508 (13067), H-89 (10010556), KH7 (13243), and L755507 (18629) were obtained from Cayman Chemicals. Z-Vad (FMK001) was obtained

(12) NLRP3 Inflammasome Priming and Activation Are Regulated by a Phosphatidylinositol-Dependent MechanismImmunoHorizonsAugust 29, 2022Hamilton C, Olona A, Leishman S, MacDonald-Ramsahai K, Cockcroft S, Larrouy-Maumus G, Anand PK.2 (COX2) inhibitor NS-398 (10 μM, 70590; Cayman), or protein kinase A (PKA) inhibitor H-89 (10 μM, 10010556; Cayman) overnight. For fatty acid supplementation, distinct fatty acids were conjugated to albumin

(13) Cyanidin 3-Glucoside Induces Hepatocyte Growth Factor in Normal Human Dermal Fibroblasts through the Activation of β(2)-Adrenergic ReceptorACS omegaJuly 5, 2022Krama A, Tokura N, Isoda H, Shigemori H, Miyamae Y.#16384-84), isoproterenol (Nacalai Tesque, Inc., Japan, #19703-04), and H89 (Cayman Chemical, United States, #10010556). Cell Culture NHDF cells, purchased from Kurabo Co., Ltd. (Japan), were cultured in Dulbecco’s modified

(14) Glycaemia and body weight are regulated by sodium-glucose cotransporter 1 (SGLT1) expression via O-GlcNAcylation in the intestineMolecular MetabolismMay 1, 2022Kimihiro Nishimura et al.For PKA inhibition or CREB inhibition, we incubated STC-1 cells in the medium with H89 at 10 μmol/l (10010556; Cayman Chemical, Ann Arbor, MI, USA) or 666-15 at 100 nmol/l (538341; Sigma-Aldrich, St. Louis, MO,

(15) C9orf72 ALS/FTD dipeptide repeat protein levels are reduced by small molecules that inhibit PKA or enhance protein degradationThe EMBO JournalJanuary 4, 2022Nausicaa V Licata et al.SelleckChem, S2449), Cilostazol (CLZ; Cayman Chemical, 15035), H89 hydrochloride (H89; Cayman Chemical, 10010556), Cycloheximide (CHX; Sigma‐Aldrich, C1988), ActinomycinD (ACTD; Sigma‐Aldrich, A9400), Dimethyl sulfoxide (DMSO

(16) Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5Nature communicationsOctober 28, 2021Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, et al.on 12 mm coverslips and incubated the following day for 30 min with 100 µM H-89 (Cayman Chemicals, 10010556) in DMSO or DMSO alone (vehicle) and H-89 was washed out with fresh medium for 5 min. Cells were fixed

(17) Phosphorylation of a chronic pain mutation in the voltage-gated sodium channel Nav1.7 increases voltage sensitivityJournal of Biological ChemistryJanuary 1, 2021Clara M. Kerth, Petra Hautvast, Jannis KÃ¶rner, Angelika Lampert, Jannis E. Meentsstored protected from light. Calphostin C and H-89 were ordered at Cayman Chemicals (Item#: 15383 and 10010556, respectively). PMA (phorbol 12-myristate 13-acetate) was purchased at Stemcell Technologies (Cat.#:

(18) Lipid Droplet-Derived Monounsaturated Fatty Acids Traffic via PLIN5 to Allosterically Activate SIRT1Molecular cellFebruary 20, 2020Najt CP, Khan SA, Heden TD, Witthuhn BA, Perez M, Heier JL, Mead LE, Franklin MP, Karanja KK, Graham MJ, et al.(ATGLi),Cayman Chemical,Cat No. 15284 EX527,Cayman Chemical,Cat No. 10009798 H89,Cayman Chemical,Cat No. 10010556 Dynabeads Protein G,ThermoFisher Scientific,Cat No. 10004D NucBlue,ThermoFisher Scientific,Cat No.

(19) β-adrenergic receptor signaling evokes the PKA-ASK axis in mature brown adipocytesPloS oneJanuary 1, 2020Hattori K, Wakatsuki H, Sakauchi C, Furutani S, Sugawara S, Hatta T, Natsume T, Ichijo H.purchased from Sigma-Aldrich. H89 was purchased from Cell Signaling Technology (#9844S) or Cayman Chemical (#10010556). 8-pCPT-cAMP (#039–18121) and H2O2 (#081–04215) were purchased from Wako. An ASK1 inhibitor, K811,

(20) Cholera toxin B induces interleukin-1β production from resident peritoneal macrophages through the pyrin inflammasome as well as the NLRP3 inflammasomeInternational immunologySeptember 18, 2019Orimo T, Sasaki I, Hemmi H, Ozasa T, Fukuda-Ohta Y, Ohta T, Morinaka M, Kitauchi M, Yamaguchi T, Sato Y, et al.hours. A pan-caspase inhibitor, Z-VAD-FMK (4800-520, MBL), and a protein kinase A (PKA) inhibitor, H-89 (10010556, Cayman Chemical), were added 3 h before addition of CTB or ATP. Dimethyl sulfoxide (DMSO, Nacalai Tesque)

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