Coelenterazine from Promega

Product Specs
ItemCoelenterazine
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberS2001
Quantity250microg

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A ribosome-bound pseudoknot in the HCV coding region stimulates viral growth by tuning viral translationCell ReportsJanuary 27, 2026Han Wan, Harim Jang, Ling Xu, Kyrillos S. Abdallah, Wendy V. Gilbert, Anna Marie Pyleplate reader with 20 μL of medium, 50 μL of 10 μM Gaussia luciferase substrate Coelenterazine (Promega S2001), and a 5-s integration time. Translation dual luciferase assay RNA preparation and transfection were

(2) Repurposing salicylic acid as a versatile inducer of proximityNature Chemical BiologySeptember 1, 2025Tianlu Wang, Siyao Liu, Yuepeng Ke, Sher Ali, Rui Wang, Tingting Hong, Ziying Liu, Guolin Ma, Tien-Hung Lan, Fen Wang, et al.incubated at 37 °C for 18 h. To assess luciferase activity, coelenterazine substrate (Promega, cat. no. S2001) was added to cells with a final concentration of 50 μM, and the signal was recorded using the Cytation 5

(3) Functional characterization of eicosanoid signaling in Drosophila developmentPLoS GenetMay 9, 2025Daiki Fujinaga, Cebrina Nolan, Naoki Yamanakamultiple well plate (Corning). The cells were then incubated with 5 µM of coelenterazine-h (Promega, #S2001) for 4 hours with gently shaking. After adding 1 ml of the assay medium, 50 µl of the cells (approximately

(4) Orphan G protein-coupled receptor GPRC5B controls macrophage function by facilitating prostaglandin E receptor 2 signalingNat CommunFebruary 7, 2025Jeonghyeon Kwon, Haruya Kawase, Kenny Mattonet, Stefan Guenther, Lisa Hahnefeld, Jamal Shamsara, Jan Heering, Michael Kurz, Sina Kirchhofer, Cornelius Krasel, et al.(2038) were from Bio-Techne GmbH. Luciferin D (122799) was purchased from PerkinElmer. Coelenterazine h (S2001) was from Promega GmbH. Butaprost (13741), L-902668 (10007712), 17-phenly trinor PGE2 (17-pt-PGE2, 14810),

(5) Functional characterization of eicosanoid signaling in Drosophila developmentbioRxivJanuary 13, 2025Daiki Fujinaga, Cebrina Nolan, Naoki Yamanakamultiple well plate (Corning). The cells were then incubated with 5 μM of coelenterazine-h (Promega, #S2001) for 4 hours with gently shaking. After adding 1 ml of the assay medium, 50 μl of the cells (approximately

(6) Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensorsSTAR ProtocolsDecember 20, 2024Remi Janicot, Mikel Garcia-Marcossubstrates for nanoluciferase, like Nano-Glo (Promega, cat# N1120) or Coelenterazine (Promega, cat# S2001), may be used for this assay. Step-by-step method details Culture of HEK293T cells and seeding for

(7) Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assaysFrontiers in ChemistryNovember 26, 2024Francesca Canyelles i Font, Krzysztof Żukowski, Masroor A. Khan, Dorota Kwiatek, Jacek L. Kolanowskibeetle luciferin (Promega # E1603), furimazine (Aobious # AOB36539), and coelenterazine (Promega # S2001). All bioluminescent signal generation experiments were performed in 384-well plate formats. The assay

(8) Large-scale deorphanization of Nematostella vectensis neuropeptide G protein-coupled receptors supports the independent expansion of bilaterian and cnidarian peptidergic systemseLifeMay 10, 2024Daniel Thiel, Luis Alfonso Yañez Guerra, Amanda Kieswetter, Alison G Cole, Liesbet Temmerman, Ulrich Technau, Gáspár Jékelyremoved and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2–3 hr. Readings were performed with a FlexStation 3 Multi-mode Microplate

(9) Specific pharmacological and Gi/o protein responses of some native GPCRs in neuronsNat CommunMarch 5, 2024Chanjuan Xu, Yiwei Zhou, Yuxuan Liu, Li Lin, Peng Liu, Xiaomei Wang, Zhengyuan Xu, Jean-Philippe Pin, Philippe Rondard, Jianfeng Liugift from Prof. Fajun Nan (Shanghai Institute of Materials Medicine, China). Coelenterazine h (Cat. S2001) and furimazine (Cat. N1120) were purchased from Promega (Madison, WI, USA). Plasmids The coding sequences

(10) COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complexPlant PhysiologyJuly 3, 2023Maria L Sorkin, Shin-Cheng Tzeng, Stefanie King, Andrés Romanowski, Nikolai Kahle, Rebecca Bindbeutel, Andreas Hiltbrunner, Marcelo J Yanovsky, Bradley S Evans, Dmitri A Nusinow10 min at room temperature with 20 µL of a 1:17 dilution of a 472 mM coelenterazine luciferin (Cat. S2001, Promega) in methanol, diluted directly into CCLR before use. Renilla luminescence was measured using

(11) Molecular insights into the regulation of constitutive activity by RNA editing of 5HT(2C) serotonin receptorsCell reportsAugust 16, 2022Gumpper RH, Fay JF, Roth BL.Potassium Chloride,Sigma,Cat#793590 Magnesium Chloride,Sigma,Cat#M0250 Talon IMAC resin,Clontech,Cat#635507 Coelenterazine,Promega,Cat#S2001 Poly-L-lysine,Sigma,Cat#P2636 Penicillin/Streptomycin,Invitrogen,Cat#15140-122 Sf-900 II SFM,Invitrogen,Cat#10902096 ESF921,Expression

(12) The RNA-binding protein QKI controls alternative splicing in vascular cells, producing an effective model for therapyJournal of cell scienceAugust 15, 2019Caines R, Cochrane A, Kelaini S, Vila-Gonzalez M, Yang C, Eleftheriadou M, Moez A, Stitt AW, Zeng L, Grieve DJ, et al.48 h after transfection, luciferase and Renilla luciferase activity was detected (Promega, #E1501; #S2001). Relative luciferase Units (RLU) were defined as the ratio of luciferase activity to Renilla luciferase

(13) A role for TSPO in mitochondrial Ca2+ homeostasis and redox stress signalingCell Death & DiseaseJune 1, 2017Jemma Gatliff, Daniel A East, Aarti Singh, Maria Soledad Alvarez, Michele Frison, Ivana Matic, Caterina Ferraina, Natalie Sampson, Federico Turkheimer, Michelangelo Campanellacells grown on glass coverslips were incubated with 5 μM coelenterazine (Promega, Madison, WI, USA, S2001) for 2 h in recording medium (RM) supplemented with 1 mM CaCl2.17 Glass coverslips were then transferred

Be the First to Write a Review!

Coelenterazine

Coelenterazine from Promega

Description

Promega

Citations (13)

Reviews

Coelenterazine from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map