Filipin III from Cayman Chemical

Product Specs
ItemFilipin III
CompanyCayman Chemical
Price $197.00Supplier Page View Company Product Page
Catalog Number70440
Quantity1 mg
FormatA crystalline solid
Molecular Weight654.8
Purity≥90%
Research AreaApoptosis, Blood, Candidiasis, Cardiovascular System, Cell Biology, Cell Death, Erythrocytes & Hemoglobin, Fungal Diseases, Immunology & Inflammation, Infectious Disease, Lipid Biochemistry, Ringworm, Sterol Lipids
Storage-20
FormulaC35H58O11
OriginISR
TypeInhibitors

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Retinal phenotype of APOB100 transgenic mice on a Western diet with human-like hyperlipidemia and cholesterol crystals in the retina and choroidLab Anim (NY)February 9, 2026Nicole El-Darzi, Tim F. Dorweiler, Natalia Mast, Julia Busik, Irina A. Pikulevaand immunohistochemistry Retinal lipids were stained as described46,49,58,93 with filipin (Cayman Chemical, no. 70440), which binds to UC46, and with BODIPY 493/503 (Thermo Fisher Scientific, D3922), which labels UC, EC,

(2) Systemic and Retinal Protective Effects of Butyrate in Early Type 2 Diabetes via Gut Microbiota-Lipid Metabolism InteractionNutrientsJuly 18, 2025Haijun Gong, Haoyu Zuo, Keling Wu, Xinbo Gao, Yuqing Lan, Ling ZhaoSolarbio, Beijing, China) was used to visualize lipid accumulation, and Filipin III (1:250 dilution, 70440, Cayman, Ann Arbor, MI, USA) was applied to the sections for 2 h at 4 °C to detect unesterified cholesterol.

(3) Investigating Psychopharmaceutical Effects on Early Vertebrate Development Using a Zebrafish Model SystemJournal of Developmental BiologyJune 27, 2025Nathan Zimmerman, Aaron Marta, Carly Baker, Zeljka Korade, Károly Mirnics, Annemarie ShibataUSA)",J67241.AP Cholesterol Detection Filipin III,"Cayman Chemical (Cayman Chemical Company, Ann Arbor, MI, USA)",70440 Cholesterol Detection Filipin III,"Cayman Chemical (Cayman Chemical Company, Ann Arbor, MI, USA)",10009867 Dimethylsulfoxide

(4) ATR promotes mTORC1 activity via de novo cholesterol synthesisEMBO ReportsJune 13, 2025Naveen Kumar Tangudu, Alexandra N Grumet, Richard Fang, Raquel Buj, Aidan R Cole, Apoorva Uboveja, Amandine Amalric, Baixue Yang, Zhentai Huang, Cassandra Happe, et al.fixed with 4% paraformaldehyde. Cells were incubated with 0.1 mg/ml filipin III (Cayman Chemical, cat# 70440) for 45–60 min. Coverslips were washed twice with PBS, mounted, and sealed. To minimize bias, imaging

(5) Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cellsCurrent BiologyApril 7, 2025Weida Wu, Alice R. Lam, Kayla Suarez, Grace N. Smith, Sarah M. Duquette, Jiaquan Yu, David Mankus, Margaret Bisher, Abigail Lytton-Jean, Scott R. Manalis, et al.were labeled with wheat germ agglutinin (WGA, Invitrogen, #W11263) and Filipin III (Cayman Chemical, #70440), respectively 53. For WGA, live cells were labeled in PBS at +4°C for 20 min, whereas for Filipin III,

(6) Small-molecule Activation of TFEB Alleviates Niemann-Pick Disease Type C via Promoting Lysosomal Exocytosis and BiogenesisElifeApril 4, 2025Kaili Du, Hongyu Chen, Zhaonan Pan, Mengli Zhao, Shixue Cheng, Yu Luo, Wenhe Zhang, Dan Listudy include SFN (Sigma-Aldrich, S4441), DMSO (Sigma-Aldrich, D2660), filipin III (Cayman Chemical, 70440), U18666A (MedChemExpress, HY-107433), Triton X-100 (MCE, HY-Y1883A), NAC (Sigma-Aldrich, A7250), FK506

(7) MoMkk1 and MoAtg1 dichotomously regulating autophagy and pathogenicity through MoAtg9 phosphorylation in Magnaporthe oryzaemBioApril 10, 2024Yun Kong, Pusheng Guo, Jiayun Xu, Jiaxu Li, Miao Wu, Ziqi Zhang, Yifan Wang, Xinyu Liu, Leiyun Yang, Muxing Liu, et al.ergosterols (Solarbio, SE8200) for 45 min and then stained with 50 µg/mL filipin (Cayman Chemical, 70440) for 4 h and observed by fluorescence microscopy with a UV filter. Before imaging, the beads were washed

(8) Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterolNature CommunicationsJanuary 11, 2024Haruka Kemmoku, Kanoko Takahashi, Kojiro Mukai, Toshiki Mori, Koichiro M. Hirosawa, Fumika Kiku, Yasunori Uchida, Yoshihiko Kuchitsu, Yu Nishioka, Masaaki Sawa, et al.Cayman); MRT67307 (19916, Cayman); 2-Bromopalmitate (320-76562, Wako); HT-DNA (D6898, Sigma); Filipin III (70440, Cayman); 25-hydroxycholesterol (11097, Cayman); OSW-1 (30310, Cayman); Cholesterol, Water Soluble (C4951,

(9) Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malariamBioDecember 19, 2023Liang R, Rao H, Pang Q, Xu R, Jiao Z, Lin L, Li L, Zhong L, Zhang Y, Guo Y, et al.spleen and brain. Cells were stained with FITC-CD8 (Biolegend, 100705) and 50 µg/mL Filipin III (Cayman, 70440) for 1 hour at room temperature. Samples were measured and analyzed using Fortessa X-20 and FlowJo,

(10) ATR promotes mTORC1 activation via de novo cholesterol synthesis in p16-low cancer cellsbioRxivNovember 2, 2023Naveen Kumar Tangudu, Zhentai Huang, Richard Fang, Raquel Buj, Apoorva Uboveja, Aidan R. Cole, Cassandra Happe, Mai Sun, Stacy L. Gelhaus, Matthew L. MacDonald, et al.fixed with 4% paraformaldehyde. Cells were incubated with 0.1mg/ml filipin III (Cayman Chemical, cat# 70440) for 45min. Coverslips were washed twice with PBS, mounted, and sealed. Abundance was assessed by integrated

(11) Inhibition of the SREBP pathway prevents SARS-CoV-2 replication and inflammasome activationLife Science AllianceNovember 1, 2023Vinicius Cardoso Soares, Suelen Silva Gomes Dias, Julia Cunha Santos, Isaclaudia G Azevedo-Quintanilha, Isabela Batista Gonçalves Moreira, Carolina Q Sacramento, Natalia Fintelman-Rodrigues, Jairo R Temerozo, Marcos Alexandre Nunes da Silva, Debora Ferreira Barreto-Vieira, et al.and incubated with 20 μM glycine for 10 min. Next, the cells were stained with 50 μg/ml filipin III (#70440; Cayman) for 2 h at room temperature in the dark. For nuclear recognition, the cells were labeled with

(12) Apolipoprotein E deficiency potentiates macrophage against Staphylococcus aureus in mice with osteomyelitis via regulating cholesterol metabolismFrontiers in Cellular and Infection MicrobiologyJuly 17, 2023Mincheng Lu, Ruiyi He, Chao Li, Zixian Liu, Yuhui Chen, Bingsheng Yang, Xianrong Zhang, Bin Yupenicillin and streptomycin and incubated at 37°C and 5% CO2. 2.4 Filipin III staining Filipin III (Cat.70440, Cayman) was dissolved in ethanol to reach a final concentration of 5 mg/mL. Cells were fixed with 4%

(13) DNA methyltransferase 1 deficiency improves macrophage motility and wound healing by ameliorating cholesterol accumulationNPJ Regenerative medicineJune 8, 2023Zhao C, Yang Q, Tang R, Li W, Wang J, Yang F, Zhao J, Zhu J, Pang W, Li N, et al.incubated with Rhodamine Phalloidin (ABclonal, RM02835, 1:200) for 30 min or 50 µg/mL filipin (Cayman, 70440) for 1.5 h. Nuclei were counterstained with DAPI for 5 min (for F-actin staining). Images of the stained

(14) SREBP activation contributes to fatty acid accumulations in necroptosis†RSC Chemical BiologyApril 5, 2023Daniel Lu, Laura R. Parisi, Omer Gokcumen, G. Ekin Atilla-GokcumenU18666A (Cat # 10009085), Betulin (Cat # 11041), necrosulfonamide (Cat # 20844) and filipin III (Cat # 70440) were obtained from Cayman Chemical. TAK-475 (Cat # SML2168), necrostatin-1s (Cat # 504297) and terbinafine

(15) Pathological changes in a model of retinal cholesterol dyshomeostasis and their mitigation by pharmacologic treatmentJournal of Lipid ResearchFebruary 1, 2023Nicole El-Darzi, Natalia Mast, Sandra S. Hammer, Tim F. Dorweiler, Julia V. Busik, Irina A. Pikulevathe retina Three dyes were used as described (31, 32). Fluorescent antibiotic filipin (catalog no.: 70440; Cayman Chemical, Ann Arbor, MI) visualized unesterified and esterified (after additional processing)

(16) Cellular cholesterol licenses Legionella pneumophila intracellular replication in macrophagesMicrobial CellJanuary 2, 2023Edna Ondari, Ashley Wilkins, Brian Latimer, Ana-Maria Dragoi, Stanimir S. Ivanovpurchased from Cayman Chemicals - u18666A (cat #10009085), ketoconazole (cat #15212), Filipin III (cat #70440), NileRed (cat # 30787). Gemcitabine (cat #G6423) and cholesterol (cat #C8667-1G) were purchased from

(17) Inflammatory stress signaling via NF-kB alters accessible cholesterol to upregulate SREBP2 transcriptional activity in endothelial cellseLifeAugust 12, 2022Fowler JWM, Zhang R, Tao B, Boutagy NE, Sessa WC.drug",EGM2,Lonza,CC-3162, "chemical compound, drug",Fatostatin,Cayman,13562, "chemical compound, drug",Filipin,Cayman,70440, "chemical compound, drug",FITC-UEAI,ThermoFisher,L32476, "chemical compound, drug",Lipopolysaccharide

(18) Polyploid giant cancer cells are dependent on cholesterol for progeny formation through amitotic divisionScientific reportsMay 27, 2022White-Gilbertson S, Lu P, Esobi I, Echesabal-Chen J, Mulholland PJ, Gooz M, Ogretmen B, Stamatikos A, Voelkel-Johnson C.lens. Alternatively, after removal of primary anti-ceramide antibody, cells were incubated with Filipin III (#70440, Cayman Chemicals), and anti-mouse FITC-conjugated secondary at 1:50 (#sc-2010, Santa Cruz). Slides

(19) The amount of membrane cholesterol required for robust cell adhesion and proliferation in serum-free conditionPloS oneJanuary 1, 2022Takii S, Wu J, Okamura D.paraformaldehyde. Cells were stained with filipin working solution (0.05 mg/ml filipin III (Cayman, 70440) in 10% FBS-contained PBS) for 2 hrs at room temperature, and washed three times in PBS, and then the

(20) Accumulation of cholesterol suppresses oxidative phosphorylation and altered responses to inflammatory stimuli of macrophagesBiochemistry and biophysics reportsDecember 1, 2021Chiu YC, Chu PW, Lin HC, Chen SK.followed by a secondary antibody for 1 h. After the incubation of secondary antibody, Filipin III (Cayman 70440) was added at 0.05 mg/ml and incubated for 2 h. Stained samples were mounted with ProLong Gold Antifade Mountant

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Filipin III

Filipin III

Filipin III from Cayman Chemical

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Cayman Chemical

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Filipin III from Cayman Chemical

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