ProLong Gold Antifade Reagent from Cell Signaling Technology

Product Specs
ItemProLong Gold Antifade Reagent
CompanyCell Signaling Technology
Price $253.00 Inquire
Catalog Number9071S
Quantity10 ml
ApplicationsIF-P, IF-F, IF-IC
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) HELZ-BRCA2 complex resolves R-loops to drive transcription-coupled homologous recombinationNat CommunJuly 23, 2026Wenjing Li, Bo Wu, Boya Gao, Elizabeth M. Irvin, Arijit Ghosh, Lillian Eliaz, Yuxin Huang, Youngho Kwon, Clara M. Stiefel, Tram Thi Ngoc Nguyen, et al.and stained with DAPI for another 15 min, before slides were mounted using antifade mounting media (9071, Cell signaling). Images were captured using a Nikon Swept Field fluorescence microscope with the 40x

(2) Transcription factors ETV4 and ETV5 are required for nephron progenitor cell maintenance, distal nephron development and connection to the collecting systemFront Cell Dev BiolJuly 1, 2026Kaitlin Day, Konrad Thorner, Milanya Thumma, Yanzhen Zhang, Evan VordemEsche, William Kuhlman, Aiden Varnell, Anhar Hosawi, Benjamin Hammond, Madison Fraunfelter, et al.washed in PBS three times and mounted in Prolong Gold Antifade Reagent (Cell Signaling Technology, 9071S). Fixed kidney explants were cut from the main filter and placed in a 24-well plate to be incubated

(3) CD4+ T Cells Sensitize Quasimesenchymal Breast Tumors Lacking CD73 to Anti-CTLA4 Immune Checkpoint Blockade TherapyCancer Res CommunJune 1, 2026Shiney Chandraganti, Caitlyn Sams, Sarthak Sahoo, Brian Feng, Isabel O’Connell, Lynna Li, Sunita Nepal, Siddhartha Pulukuri, Kimaya Bakhle, Prathapan Thiru, et al.distilled water and mounted with ProLong Gold Antifade mounting media (Cell Signaling Technologies 9071S). Microscopy The stained sections were viewed under a Zeiss AxioObserver inverted microscope with

(4) Reactivation of DRP1 plays a functional role in resistance to MEK inhibition in pancreatic cancer cellsbioRxivMay 25, 2026Salma Sharmin, Jennifer A. Kashatus, Sara J. Adair, Emma Bakall Loewgren, Mohammad Fallahi-Sichani, Todd W. Bauer, David F. Kashatusdilution. After the final wash, slides were mounted immediately in Prolong Gold antifade reagent (CST #9071). A Zeiss LSM 900 with Airyscan2 confocal microscope was used for imaging mitochondrial network using

(5) Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cellsPLOS BiologyApril 27, 2026Jian Guan, Rebecca Lee Hurto, Akash Rai, Janakraj Bhattrai, Christopher A. Azaldegui, Luis A. Ortiz-Rodríguez, Quancheng Liu, Julie S. Biteen, Lydia Freddolino, Ursula JakobCoverslips were washed three times in PBS, sealed on a glass slide with a drop of antifade reagent (#9071S, Cell Signalling) and imaged as previously described. Four separate imaging fields were imaged and analyzed

(6) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and immunityApril 15, 2026Schaaf D, Dresen M, Weldearegay YB, Biermann J, Brockmeier SL, Baumgärtner W, Jarek M, Valentin-Weigand P.membrane was cut out and mounted with ProLong Gold Antifade Reagent (Cell Signaling Technology, Cat. No. 9071), as previously described (19). PCLS were fixed with 4% (vol/vol) phosphate-buffered formalin (Department

(7) HP-Bodies – Ancestral Condensates that Regulate RNA Turnover and Protein Translation in BacteriabioRxivApril 11, 2026Jian Guan, Rebecca Lee Hurto, Akash Rai, Christopher A. Azaldegui, Luis A. Ortiz-Rodríguez, Julie S. Biteen, Lydia Freddolino, Ursula JakobCoverslips were washed three times in PBS, sealed on a glass slide with a drop of antifade reagent (#9071S, Cell Signalling) and imaged as previously described. Single-molecule fluorescence microscopy. WT

(8) A disease-causing isoleucyl-tRNA synthetase variant leads to altered protein complex formation and cellular stress responseJournal of Biological ChemistryMarch 1, 2026Han Gao, Rasangi Tennakoon, Felicia Pais Araújo, Jolie M. Miller, Samuel Protais Nyandwi, Qingyu Shi, Juan Pablo Padilla-Martínez, Hui Peng, Haissi Cuiwater and mounted on glass slides using ProLong Gold Antifade Reagent (Cell Signaling Technologies, #9071S) overnight at room temperature, protected from light. Samples were sealed with nail polish and stored

(9) Mitochondrial transfer from immune to tumor cells enables lymph node metastasisCell MetabolismFebruary 3, 2026Azusa Terasaki, Keshav Bhatnagar, Alexis T. Weiner, Yuhao Tan, Viktoria Szeifert, Han-Li Huang, Lukas Wiggers, Viviana Rodrigues, Cara C. Rada, Vishnu Shankar, et al.Biologicals/Bio-Techne),Cat#H-2000-10 ProLong Gold Antifade Reagent,Cell Signaling Technology,Cat#9071 "Corning 100 mL 0.5M EDTA, pH 8.0",Corning,Cat#46-034-CI "Trypsin 0.25% protease with porcine trypsin,

(10) In vivo and in vitro susceptibility and inflammatory response of postnatal mouse cortical neurons and glial cells to zika virus infectionPLoS OneDecember 31, 2025María-Angélica Calderón-Peláez, Myriam L. Velandia-Romero, Jaime E. Castellanostissues, 1:5000 for cells, B2261, Sigma-Merck), and sections were mounted with ProLong Gold antifade (#9071, Cell signaling). Observations were made under a Zeiss M2 microscope, coupled to the Colibri 7 fluorescence

(11) Cannabidiol Mitigates Pollution-Induced Inflammatory, Oxidative, and Barrier Damage in Ex Vivo Human SkinBiomoleculesDecember 20, 2025Klinngam W, Loruthai O, Vimolmangkang S.counterstained with DAPI (Abcam, UK) for 15 min. Slides were mounted with ProLong™ Gold Antifade Mountant (#9071S; Cell Signaling Technology, Danvers, MA, USA) and imaged at 400× magnification using a Leica TCS SP8

(12) Fibroblast-like cells in mesothelioma can derive from tumor cellsCell Death & DifferentiationDecember 16, 2025Jose M. Garcia-Manteiga, Eltjona Rrapaj, Francesca Caprioglio, Francesco De Marchis, Andrea Lamarca, Liam S. Colley, Angelo Carretta, Daniela Finocchiaro, Francesca Mercalli, Annamaria Molinario, et al.counterstained using DAPI. Slides were mounted with ProLong Gold Antifade Reagent (Cell Signaling #9071). Images were acquired using a SP8 laser scanning confocal microscope (Leica). mIHC image analysis Individual

(13) Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivoPLOS OneNovember 26, 2025María-Angélica Calderón-Peláez, Myriam L. Velandia-Romero, J. Manuel Matiz-González, Jaime E. CastellanosScientific) and DAPI (Merck, 10236276001) were applied before mounting with ProLong Gold Antifade Reagent (9071, Cell Signaling). Images were acquired on a Zeiss Axioimager M2 microscope with Colibri 7 fluorescence

(14) Visualization of liquid-liquid phase transitions using a tiny G-quadruplex binding proteinNature CommunicationsSeptember 29, 2025Bikash R. Sahoo, Xiexiong Deng, Ee Lin Wong, Nathan Clark, Harry J. Yang, Alexey Kovalenko, Vivekanandan Subramanian, Bryan B. Guzman, Sarah E. Harris, Budheswar Dehury, et al.more times with 1X DPBS, air dried, and mounted with ProLongGoldTM antifade reagent (Cell Signaling, 9071S) on glass slides. After 24 h of mounting, the slides were sealed with nail polish and imaged using a

(15) The Tacrine-Induced Endoplasmic Reticulum Stress in AChE-Expressed Cells Leads to Improper Assembly and Transport of the Oligomeric Enzyme: Reversal by TrehaloseJournal of neurochemistryAugust 1, 2025Wang X, Xia Y, Guo MS, Wu J, Dilidaer A, Gao J, Dong TT, Zhu Y, Tsim KWK.cells were mounted with ProLong Gold Antifade reagent with 4′,6‐diamidino‐2‐phenylindole (DAPI) (Cat# 9071, Cell Signaling Technology) and then subjected to visualization by SP8 confocal microscope (Leica Microsystems,

(16) Preclinical Evaluation of an Integrin αvβ6-Targeted Photodynamic TherapyBioconjugate ChemistryJuly 16, 2025Hua Zhang, Tanushree Ganguly, Rebecca Harris, Ryan A. Davis, Sven H. Hausner, Luciana Kovacs, Julie L. Sutcliffefor 10 min, washed three additional times with PBS, and mounted with ProLong Gold Antifade reagent (#9071, Cell Signaling Technology, Danvers, MA). Cells were imaged on a Leica TCS SP8 confocal microscope (Ex

(17) Probing condensate microenvironments with a micropeptide killswitchNatureJune 4, 2025Yaotian Zhang, Ida Stöppelkamp, Pablo Fernandez-Pernas, Melanie Allram, Matthew Charman, Alexandre P. Magalhaes, Melanie Piedavent-Salomon, Gregor Sommer, Yu-Chieh Sung, Katrina Meyer, et al.PBS and mounted onto glass slides using ProLong Gold Antifade Reagent (Cell Signaling Technologies, 9071). The following primary antibodies were used: 52K (gift from P. Hearing80; rabbit, polyclonal, 1:500)

(18) CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapybioRxivMay 16, 2025Shiney Chandraganti, Caitlyn Sams, Sarthak Sahoo, Brian Feng, Isabel O’Connell, Lynna Li, Sunita Nepal, Siddhartha Pulukuri, Kimaya Bakhle, Prathapan Thiru, et al.distilled water and mounted with Prolong Gold anti-Fade mounting media (Cell Signaling Technologies 9071S). Microscopy The stained sections were viewed under a Zeiss AxioObserver inverted microscope with

(19) Unraveling the effects of uric acid on endothelial cells: A global proteomic studyRedox BiolApril 1, 2025Bianca Dempsey, Beatriz Pereira da Silva, Litiele Cezar Cruz, Danielle Vileigas, Amanda R.M. Silva, Railmara Pereira da Silva, Flavia Carla Meottiwashes, coverslips were mounted on microscope objective slides using ProLongGold Antifade Reagent (9071, Cell Signaling, Danvers, Massachusetts, USA). Images were acquired with a Leica SP8 laser scanning

(20) Hippocampus Oxytocin Signaling Promotes Prosocial Eating in RatsBiological PsychiatryMarch 1, 2025Jessica J. Rea, Clarissa M. Liu, Anna M.R. Hayes, Alexander G. Bashaw, Grace M. Schwartz, Rita Ohan, Léa Décarie-Spain, Alicia E. Kao, Molly E. Klug, Kenneth J. Phung, et al.L4408-100G, Chemical Compound or Drug,Prolong Gold AntiFade Reagent,Cell Signaling Technology,Cat#9071s, Commercial Assay Or Kit,RNAscope Fluorescent Multiplex Detection Reagent Kit,Advanced Cell Diagnostics,Cat#323100, Commercial

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