MEM α, nucleosides from Thermo Fisher Scientific

Product Specs
ItemMEM α, nucleosides
CompanyThermo Fisher Scientific
Price $39.50Supplier Page View Company Product Page
Catalog Number12571063
Quantity500 mL
FormatLiquid
Volume500 ml
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesisBioactive MaterialsJune 1, 2026Qiji Lu, Yudi Kuang, Jingjing Diao, Jiaqian Zheng, Naru Zhao, Chang Du, Yingjun WangChina). The reagents used in the experiment included: H-DMEM(11965118, Gibco, USA.), α-DMEM medium(12571063, Gibco, USA.), TritonX-100(ST1723, Beyotime, China), 4 % paraformaldehyde (BL539A, Biosharp, China),FBS(A5256701,

(2) Biocompatibility, inflammatory response, and antimicrobial properties of single-bottle adhesives on gingival fibroblast and human dental pulp stem cellsSci RepApril 30, 2026Amir Hafez Ibrahim, Shereen Shaaban Mustafa, Nashwa El-Khazragy, Shereen Hafez Ibrahimfrom the resulting cell suspension by centrifugation and then resuspended in α-MEM, (Catalog Number: 12571063; Gibco, Thermo Fisher), a complete culture medium supplemented with 10–20% fetal bovine serum (FBS;

(3) FOXE1 promotes the progression of pulp inflammation by activating PANoptosis in dental pulp cellsiScienceApril 17, 2026Fan Gu, Ruiqi Li, Delan Huang, Linlin Peng, Tingting Huan, Kaili Ye, Zhuan Bian, Wei Yinproteins","Chemicals, peptides, and recombinant proteins" ,, Minimum Essential Medium α (α-MEM),Gibco,Cat#12571063 Phosphate-Buffered Saline (PBS),Gibco,Cat#10010023 Penicillin-Streptomycin (PS),Gibco,Cat#15140122 Fetal

(4) Bone Multicellular Unit on a Chip (BMU-Chip) Subjected to Cyclic Mechanical LoadingACS Biomaterials Science & EngineeringApril 13, 2026Anna-Blessing Merife, Michael P. Seitz, Angelika Polshikova, Ujjwal Aryal, Zachary J. Geffert, Era Jain, Jason Horton, Paola Divieti Pajevic, Pranav Somanincorporating the osteocyte cell solution. Subconfluent OCY454 osteocytic cells were cultured in alpha-MEM (#12571063, Gibco) containing l-glutamine, 1% Anti-Anti (Life Technologies, #15240), and 10% fetal bovine serum

(5) Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitroNat CommunApril 8, 2026Teodora Piskova, Aleksandra N. Kozyrina, Giedrė Astrauskaitė, Mohamed Elsafi Mabrouk, Sebastian Schepl, Stacy Lok Sze Yam, Ragul Ravithas, Wolfgang Wagner, Massimo Vassalli, Jacopo Di Russovitronectin XF (StemCell Technologies, 07180). Cells were cultured in MEM-alpha with nucleosides (Thermo Fisher, 12571063), supplemented with 1x Glutamax (Thermo Fisher, 35050061), 5% (vol/vol) Knockout Serum replacement (Gibco,

(6) A human synovial tendon-on-a-chip models key features of peritendinous adhesions and offers a new approach methodology for testing anti-fibrotic drugsbioRxivApril 7, 2026Isabelle Linares, Anabelle Creveling, Abdikadir Osman, Nathan Grandwetter, Benjamin L. Miller, James L. McGrath, Hani A. Awaddigital flexor tendon. Tendon fibroblasts were expanded in T-175 flasks in MEM Alpha media (Gibco, 12571063) supplemented with 10% FBS and 1% Penicillin-Streptomycin and verified for scleraxis expression using

(7) Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic systembioRxivApril 4, 2026Hyopil Kim, Cheng Xu, Craig Washington, Caleb Shi, Maggie Lowman, Justus M. Kebschullwere maintained in medium containing 5% FBS (Gibco, 16140071 or Cytiva, SH30070.03), MEM Alpha (Gibco, 12571063), 1x Antibiotic-Antimycotic (Gibco, 15240096), 1x MEM Vitamin (Gibco, 11120052), and 1x L-Glutamine

(8) MAPseq2: a sensitive and cost-effective barcoded connectomics methodbioRxivApril 4, 2026Hyopil Kim, Huihui Qi, Craig Washington, Xiaoping Liang, Justus M. Kebschull(Invitrogen, 11668027) at a 1:1 molar ratio. BHK cells were cultured in a medium composed of MEM Alpha (Gibco, 12571063), 5% FBS (Cytiva, SH30070.03), 1x Antibiotic-Antimycotic (Gibco, 15240096), 1x MEM Vitamin Solution

(9) Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axisBioact MaterApril 3, 2026Yu Xu, Wenpeng Xu, Wencan Zhang, Shangzhi Li, Dingxin Zhang, Xiangyu Lin, Qian Sun, Yanguo Wang, Peijie Hu, Haipeng Siwere flushed out into a 10-cm culture dish using a syringe with a 25G needle and suspended in α-MEM (12571063, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, F101, Vazyme, Nanjing,

(10) Isolation, genomic characterization, and pathogenicity of an emerging PEDV variant in Korea related to virulent Chinese strainsFront Vet SciApril 1, 2026Dong-Kyu Lee, Jonghyun Park, Seung-Chai Kim, Hwan-Ju Kim, Jung-Hoon Kwon, Young S. Lyoo, Won-Il Kim, Choi-Kyu Park, Hye-Ryung Kimgrowth kinetics Vero cells (ATCC CCL-81) were maintained in α-minimum essential medium (α-MEM; Gibco, 12571063) supplemented with 5% fetal bovine serum (FBS; Gibco, A5670701) and 1% antibiotic–antimycotic (Gibco,

(11) Uncovering cancer dependencies in peptide-interacting protein pocketsbioRxivMarch 25, 2026Mihkel Örd, Liz Kogan, Devin Bradburn, Michelle Leiser, Mardo Kõivomägi, Norman E. Davey, Pau Creixell(41966029, Gibco), K562 in IMDM (12440053, Gibco), and OCIAML2 and OCIAML3 in MEM ɑ with nucleosides (12571063, Gibco). All media were supplemented with 10% fetal bovine serum (A5209402, Gibco), 100 U/ml penicillin,

(12) Multi-omics and causal inference reveal lactylation-based osteoblast regulatory networks and drug candidatesiScienceMarch 20, 2026Ruijing Chen, Siliang Ge, Feifan Chang, Ming Chen, Yi Li, Yihui Li, Desheng You, Peifu Tang, Pengbin Yinproteins" ,, FBS,GIBCO,Cat#: 10091-148 Trypsin,GIBCO,Cat#: 25200072 PBS,GIBCO,Cat#: 10010001 MEM α,GIBCO,Cat#: 12571063 Penicillin-Streptomycin,GIBCO,Cat#: 15140122 β-glycerol phosphonate,Sigma-Aldrich,Cat#: G9422 Type

(13) Silk cryogel and electrospun scaffold characterization for bone-tendon interface applicationsFront Bioeng BiotechnolMarch 17, 2026Amritha Anup, Milenka Men, Katelyn Wasacz, Michelle Bok, Afton K. Limberg, Katherine R. Hixon(enthesis) end (Dudakovic et al., 2024). Dissected tendons were immediately placed in a solution of αMEM (12571063, Thermo Fisher Scientific) and 2 mg/mL collagenase type 1 (9001-12–1, Worthington Biomedical Corporation).

(14) Silk cryogel and electrospun scaffold characterization for bone-tendon interface applicationsFrontiers in Bioengineering and BiotechnologyMarch 17, 2026Amritha Anup, Milenka Men, Katelyn Wasacz, Michelle Bok, Afton K. Limberg, Katherine R. Hixon(enthesis) end (Dudakovic et al., 2024). Dissected tendons were immediately placed in a solution of αMEM (12571063, Thermo Fisher Scientific) and 2 mg/mL collagenase type 1 (9001-12–1, Worthington Biomedical Corporation).

(15) Fibroblast-derived exosomes induce macrophage pyroptosis to mediate orthodontic bone remodeling through the mtDNA/NLRP3 axisJournal of NanobiotechnologyMarch 14, 2026Qianyao Yu, Kunyao Guo, Yuhui Yang, Hao Liu, Yineng Han, Xinlei Yu, Jiayi Wang, Yi Zhao, Xinyu Su, Kaixi Zhu, et al.RRID: CVCL_0006) in October 2022. PDLFs were cultured in modified Eagle’s medium (Gibco, USA, Cat. No. 12571063) supplemented with 10% fetal bovine serum (FBS, Procell, China, Cat. No. 164210) and 1% penicillin/streptomycin

(16) TSPO governs bone-lipid homeostasis by redirecting BMSC differentiation via the PI3K/AKT/β-catenin pathwayStem Cell Research & TherapyMarch 11, 2026Peng Zhang, Hongyu Zheng, Zhao Lin, Minjuan Zhang, Linhai Yang, Zhibo Deng, Chao Song, Hanhao Dai, Yibin Su, Rongsheng Zhang, et al.marrow was aspirated from the medullary cavity using a syringe and transferred into complete MEM α (12571063, Gibco, USA) supplemented with 10% FBS and 1% penicillin/streptomycin. The resulting cell suspension

(17) Bone Morphogenetic Protein (BMP) 9 Outperforms BMP2 in Osteogenesis and Osseointegration: In Vitro and In VivoClin Implant Dent Relat ResMarch 10, 2026Hee-Seung Han, Dong Woo Lee, Yu-Bin Kim, Sanghui Seok, Jeong Yeon Park, Sungtae Kim, Jue Yeon Lee, Yoon Jeong Park, Chong Pyung Chung, Young-Dan ChoUK). 2.2 Cell Culture and Treatment MC3T3‐E1 pre‐osteoblast cells and hPDLSCs were cultured in α‐MEM (12571063, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 100 U/mL penicillin,

(18) comBO: A combined human bone and lympho-myeloid bone marrow organoid for preclinical modeling of hematopoietic disordersCell Stem CellMarch 5, 2026Yuqi Shen, Camelia Benlabiod, Edmund Watson, Kristian Gurashi, Alex Fower, Antonio Rodriguez-Romera, Jasmeet S. Reyat, Shady Adnan-Awad, Rupen Hargreaves, Samuel Kemble, et al.(Insulin-Transferrin-Selenium-Ethanolamine (ITS -X)),Gibco,Cat#51500056 alpha-MEM media,Gibco,Cat#12571063 HEPES,Gibco,Cat#15630080 b-mercaptoethanol,Gibco,Cat#21985023 penicillin-streptomycin-glutamine,Gibco,Cat#10378016 RPMI-1640,Gibco,Cat#11875093 L-ascorbic

(19) Myofibroblast lineage mapping and inhibiting subretinal fibrosis by targeting SMAD3 and MRTF pathways via microRNA-24 functional studybioRxivMarch 4, 2026Yinga Wu, Yao Tong, Katherine G. Byrnes, Qi Zhou, Chunmin Dong, Chase Benjamin, Emma Parker, Duran Bao, Zhaoyang Ren, Chastain A. Anderson, et al.differentiate human RPE cells using nicotinamide33, ARPE-19 cells were cultured in MEM alpha medium (Gibco, 12571063) supplemented with GlutaMAX™ Supplement (ThermoFisher, 35050061), 1% fetal bovine (HyClone), 1% Penicillin-Streptomycin (10,000 U/mL

(20) Canonical BAF chromatin remodeling complex specifies stem cell fate via cell-type-specific co-factor recruitmentNature CommunicationsMarch 3, 2026Mingyi Zhang, Jifan Feng, Tingwei Guo, Lin Meng, Fei Pei, Lu Gao, Yuchen Yang, Thach-Vu Ho, Yang Chaimg/mL dispase II (Sigma-Aldrich, 54905400) was added to the chopped tissue in α-MEM medium (Gibco, 12571063). After fragmentation, the tissue was incubated at 37 °C in a thermomixer (Eppendorf) at 600 rpm for

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MEM α, nucleosides from Thermo Fisher Scientific

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MEM α, nucleosides from Thermo Fisher Scientific

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