DRAQ7 ™ from Cell Signaling Technology

Product Specs
ItemDRAQ7 ™
CompanyCell Signaling Technology
Price $351.00 Inquire
Catalog Number7406S
Quantity1 ml
Research AreaRelated Products
ApplicationsFC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Single-cell epigenomics uncovers heterochromatin instability and transcription factor dysfunction during mouse brain agingCell ReportsMarch 24, 2026Maria Luisa Amaral, Sainath Mamde, Michael Miller, Xiaomeng Hou, Jessica Arzavala, Julia Osteen, Nicholas D. Johnson, Elizabeth Walker Smoot, Qian Yang, Emily Eisner, et al.nuclei from individual plates were pooled together, stained nuclei suspension with 3 μM Draq7 (1:100, 7406, Cell Signaling). Sort 20 nuclei per well into eight 96-well plates (total of 768 wells, 15,360 nuclei

(2) RIPK1 autophosphorylation at S161 mediates cell death and inflammationThe Journal of experimental medicineDecember 1, 2025Koerner L, Li X, Silnov E, Laurien L, Pasparakis M.inhibitor 5Z-7 Oxozeaenol (O9890; Sigma-Aldrich, 0.25 µM) in the presence of the dead cell stain DRAQ7 (7406; Cell Signaling, 0.15 µM) for 30 min before addition of recombinant mouse TNF (VIB Protein Service Facility,

(3) Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during agingNature CommunicationsNovember 14, 2025Dragana Stefanovska, Eliza Sassu, Mehmet Tekman, Amirhossein Naghsh Nilchi, Severin Haider, Claudia Domisch, Madelon Hossfeld, Stefanie Perez-Feliz, Lauritz Miarka, Franziska Schneider-Warme, et al.Finally, the nuclei were filtered again through a 30 μm CellTrics (Sysmex, MSA150914), and DRAQ7 (#7406, Cell Signaling) was added to the nuclei suspension for nuclei detection by flow cytometry. 10,000-20,000

(4) Single-cell multiome and spatial profiling reveals pancreas cell type–specific gene regulatory programs of type 1 diabetes progressionScience AdvancesSeptember 12, 2025Rebecca Melton, Sara Jimenez, Weston Elison, Luca Tucciarone, Abigail Howell, Gaowei Wang, Denise Berti, Elisha Beebe, Michael Miller, Chun Zeng, et al.in 2% BSA (Sigma-Aldrich, SRE0036) in PBS] and stained with DRAQ7 (1:100; Cell Signaling Technology, 7406). A total of 75,000 nuclei were sorted using an SH800 sorter (Sony) into 50 μl of collection buffer

(5) Activation of lysosomal iron triggers ferroptosis in cancerNatureJune 1, 2025Cañeque T, Baron L, Müller S, Carmona A, Colombeau L, Versini A, Solier S, Gaillet C, Sindikubwabo F, Sampaio JL, et al.averaged. Cell death was measured on the basis of the incorporation of DRAQ7 (0.1 µM, Cell Signaling, 7406S). Data were collected as count of DRAQ7-positive cells per total number of cells in each condition. Generation

(6) Single-Cell Epigenomics Uncovers Heterochromatin Instability and Transcription Factor Dysfunction during Mouse Brain AgingbioRxivMay 25, 2025Maria Luisa Amaral, Sainath Mamde, Michael Miller, Xiaomeng Hou, Jessica Arzavala, Julia Osteen, Nicholas D. Johnson, Elizabeth Walker Smoot, Qian Yang, Emily Eisner, et al.nuclei from individual plates were pooled together, stained nuclei suspension with 3 μM Draq7 (1:100, 7406, Cell Signalling). Sort 20 nuclei per well into eight 96-well plates (total of 768 wells, 15,360 nuclei

(7) Transplantation of gasdermin pores by extracellular vesicles propagates pyroptosis to bystander cellsCellJanuary 23, 2025Wright SS, Kumari P, Fraile-Ágreda V, Wang C, Shivcharan S, Kappelhoff S, Margheritis EG, Matz A, Vasudevan SO, Rubio I, et al.Scientific,Cat# A39267 Propidium iodide (PI),Sigma-Aldrich,Cat# P4864 DRAQ7,Cell Signaling Technologies,Cat# 7406S CellBrite488,Biotium,Cat# 30106 AF568-conjugated phalloidin,Invitrogen,Cat# A12380 AF488-conjugated

(8) Park7 deletion leads to sex-specific transcriptome changes involving NRF2-CYP1B1 axis in mouse midbrain astrocytesNPJ Parkinsons DisJanuary 4, 2025Sergio Helgueta, Tony Heurtaux, Alessia Sciortino, Yujuan Gui, Jochen Ohnmacht, Pauline Mencke, Ibrahim Boussaad, Rashi Halder, Pierre Garcia, Rejko Krüger, et al.with PBS1X for 5 min at RT. Each tissue section was then covered by DAPI or DRAQ7 (Cell Signaling, # 7406) for 30 s at RT. After removing DAPI or DRAQ7, sections were immediately covered by 1-2 drops of ProLong Gold Antifade Mountant

(9) Rapid Biofabrication of an Advanced Microphysiological System Mimicking Phenotypical Heterogeneity and Drug Resistance in GlioblastomaAdvanced Healthcare MaterialsDecember 1, 2024Sirjana Pun, Anusha Prakash, Dalee Demaree, Daniel Pomeranz Krummel, Giuseppe Sciumè, Soma Sengupta, Riccardo BarrileThermo Fisher Scientific; #H3570), DRAQ7 (1:100 working solution from a 0.3 mM stock, Cell Signaling Technology; #7406), and Calcein (1 µL mL−1 working solution from a 2.5 mg mL−1 stock, Invitrogen; C3100MP), and then imaged

(10) ZBP1 causes inflammation by inducing RIPK3-mediated necroptosis and RIPK1 kinase activity-independent apoptosisCell death and differentiationJuly 1, 2024Koerner L, Wachsmuth L, Kumari S, Schwarzer R, Wagner T, Jiao H, Pasparakis M.2263-5-BV) or GSK’872 (3 µM Millipore). Cell death was measured by Draq7 (0.15 µM, Cell Signaling, 7406) incorporation, normalized to Draq5 (40845, Cell Signaling) staining and analyzed using the IncuCyte

(11) Single-nucleus genomics in outbred rats with divergent cocaine addiction-like behaviors reveals changes in amygdala GABAergic inhibitionNature NeuroscienceNovember 1, 2023Jessica L. Zhou, Giordano de Guglielmo, Aaron J. Ho, Marsida Kallupi, Narayan Pokhrel, Hai-Ri Li, Apurva S. Chitre, Daniel Munro, Pejman Mohammadi, Lieselot L. G. Carrette, et al.(Sigma-Aldrich, catalog no. SRE0036) in PBS) and stained with DRAQ7 (1:100; Cell Signaling, catalog no. 7406). Up to 75,000 nuclei were sorted using a SH800 sorter (Sony Cell Sorter Software v.2.1.2-5) into 50

(12) AKT activation because of PTEN loss upregulates xCT via GSK3β/NRF2, leading to inhibition of ferroptosis in PTEN-mutant tumor cellsCell reportsMay 30, 2023Cahuzac KM, Lubin A, Bosch K, Stokes N, Shoenfeld SM, Zhou R, Lemon H, Asara J, Parsons RE.Media,corning,10–090-CMR MEM Media,corning,10–009-CVR Ham’s F12 Media,corning,10–080-CV Trypsin,Cell Signaling,7406S Penicillin-Streptomycin,Fisher,30002ci Fetal Bovine Serum,Gibco,A52567-01 Rapamycin,Sigma,R8781 Torin1,Sigma,"475,991" CHIR99021,Sigma,SML1046 AZD5363,Selleck

(13) Characterization of altered molecular mechanisms in Parkinson’s disease through cell type–resolved multiomics analysesScience AdvancesApril 14, 2023Andrew J. Lee, Changyoun Kim, Seongwan Park, Jaegeon Joo, Baekgyu Choi, Dongchan Yang, Kyoungho Jun, Junghyun Eom, Seung-Jae Lee, Sun Ju Chung, et al.04-0042-2316) to remove excessive debris. The nuclei were stained with DRAQ7 (1:100; Cell Signaling Technology, 7406) for snRNA-seq and with 4′,6-diamidino-2-phenylindole (10 μg/ml; Sigma-Aldrich, 32670) for snATAC-seq.

(14) Single-cell epigenome analysis reveals age-associated decay of heterochromatin domains in excitatory neurons in the mouse brainCell ResearchOctober 7, 2022Yanxiao Zhang, Maria Luisa Amaral, Chenxu Zhu, Steven Francis Grieco, Xiaomeng Hou, Lin Lin, Justin Buchanan, Liqi Tong, Sebastian Preissl, Xiangmin Xu, et al.(Promega, N211B), 2% BSA (Sigma-Aldrich, SRE0036) in PBS and stained with DRAQ7 (1:100; Cell Signaling, 7406). 75,000 nuclei were sorted using a SH800 sorter (Sony) into 50 µL of collection buffer consisting of

(15) Microglia phenotypes are associated with subregional patterns of concomitant tau, amyloid-β and α-synuclein pathologies in the hippocampus of patients with Alzheimer's disease and dementia with Lewy bodiesActa neuropathologica communicationsMarch 16, 2022Fixemer S, Ameli C, Hammer G, Salamanca L, Uriarte Huarte O, Schwartz C, Gérardy JJ, Mechawar N, Skupin A, Mittelbronn M, et al.were incubated for an additional 20 min with the fluorescent DNA dye DRAQ7™ (1:100, Cell Signaling #7406) at RT and washed twice for 10 min in 0.1 M PB (pH 7.4) before mounting. See Tables 2 and 3 for references

(16) Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5⁺ basal cellsNature cell biologyJanuary 1, 2022Kathiriya JJ, Wang C, Zhou M, Brumwell A, Cassandras M, Le Saux CJ, Cohen M, Alysandratos KD, Wang B, Wolters P, et al.Thermo Fisher; 1:1000). Doublets and dead cells were excluded based on forward and side scatters and DRAQ7 (Cat#7406S; Cell Signaling; 1:200) or DAPI fluorescence. hAEC2s were sorted as live/EpCAM+/HTII-280+ cells, and

(17) Design, Synthesis, and Evaluation of Potent, Selective, and Bioavailable AKT Kinase DegradersJournal of medicinal chemistryDecember 23, 2021Yu X, Xu J, Xie L, Wang L, Shen Y, Cahuzac KM, Chen X, Liu J, Parsons RE, Jin J.Biosciences) on live cells over time. For the measurement of cell apoptosis, DRAQ7 (Cell Signaling # 7406) at 1.5 μM was included in the medium and apoptotic red counts were measured in an IncuCyte FLR automated

(18) AKT Degradation Selectively Inhibits the Growth of PI3K/PTEN Pathway-Mutant Cancers with Wild-Type KRAS and BRAF by Destabilizing Aurora Kinase BCancer discoveryDecember 1, 2021Xu J, Yu X, Martin TC, Bansal A, Cheung K, Lubin A, Stratikopoulos E, Cahuzac KM, Wang L, Xie L, et al.(Essen Biosciences) on live cells over time. For measurement of cell death DRAQ7 (Cell Signaling # 7406) at 1.5 μM was included in the medium and apoptotic red counts were measured in IncuCyte™ FLR automated

(19) A single-cell atlas of chromatin accessibility in the human genomeCellNovember 24, 2021Kai Zhang, James D. Hocker, Michael Miller, Xiaomeng Hou, Joshua Chiou, Olivier B. Poirion, Yunjiang Qiu, Yang E. Li, Kyle J. Gaulton, Allen Wang, et al.Macrolab at UC Berkeley,N/A SPRISelect reagent,Beckman Coulter,Cat# B23319 DRAQ7,Cell Signaling,Cat# 7406 NEBNext High-Fidelity PCR Master Mix,topNEB,Cat# M0541L LightCycler 480 SYBR Green I Master Mix,Roche,Cat#

(20) Cardiac cell type–specific gene regulatory programs and disease risk associationScience AdvancesMay 14, 2021James D. Hocker et al.in 2% BSA (Sigma-Aldrich, SRE0036) in PBS] and stained with DRAQ7 (1:100; Cell Signaling Technology, 7406). Seventy-five thousand nuclei were sorted using an SH800 sorter (Sony) into 50 l of collection buffer

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5/25/2017DRAQ7 Is A Great Choice For Excluding Dead Cells In Flow Cytometry Alexis BrumwellThis product is fantastic for excluding dead cells in flow cytometry. After doing a lung epithelial cell prep, we resuspend the cells at 10^7/ml in DMEM + 2%FBS and add DRAQ7 at 1:200. The dead cells... Read Review

DRAQ7 ™

DRAQ7 ™ from Cell Signaling Technology

Cell Signaling Technology

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DRAQ7 ™ from Cell Signaling Technology

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