2X Laemmli Sample Buffer from Santa Cruz Biotechnology, Inc.

Product Specs
Item2X Laemmli Sample Buffer
CompanySanta Cruz Biotechnology, Inc.
Price $12.00Supplier Page View Company Product Page
Catalog Numbersc-286962
Quantity30 ml
TypeBuffers

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titersMolecular Therapy AdvancesMarch 12, 2026Stephen M. Winston, Mark A. Brimble, Kristin B. Wiggins, Isaiah L. Reeves, Yunyu Spence, Baochang Fan, Shaoyuan Tan, Christopher L. Morton, Pei-Hsin Cheng, Mary Y. Spence, et al.and FBS and were then lysed in culture using 2× Laemmli buffer (Santa Cruz Biotechnology, cat. no. sc-286962). For 6-well plate assays, samples were lysed in 200 μL of Laemmli buffer, sonicated, and heated at

(2) HPV16 E6 oncoprotein promotes microhomology-mediated viral integration by increasing PolΘ protein expressionProc Natl Acad Sci U S AMarch 10, 2026Guangli Zhu, Shuhei Asada, Jithma P. Abeykoon, Lifang Sun, Sirisha Mukkavalli, Alan D. D'Andrea#8553S). Proteins were denatured by boiling at 70 °C for 10 min in 2× Laemmli sample buffer (Santa Cruz, #sc-286962), separated on NuPAGE Tris-Acetate gels (Invitrogen #EA0375BOX) or Bis-Tris gels (Invitrogen #NP0321BOX),

(3) Oncostatin M orchestrates collective epithelial migration via HIF1A activationbioRxivJanuary 16, 2026Ian C. McLean, Sean M. Gross, Tiera A. Liby, Mark A. Dane, Daniel S. Derrick, Laura M. Heisertreatment with OSM EGF, or CoCl2 cells were lysed in hot Laemmli buffer (Santa Cruz Biotechnology #sc-286962). Cell lysates were boiled at 95C for 10 minutes, then total protein concentration was determined and

(4) Defective Microhomology-Mediated End-joining in SMARCB1-Deficient TumorsbioRxivJanuary 16, 2026Guangli Zhu, Shuhei Asada, Huy Nguyen, Yuna Hirohashi, Lifang Sun, Avneesh Saravanapavan, Sirisha Mukkavalli, Geoffrey I. Shapiro, Alan D. D’Andreasamples were denatured by boiling at 70 °C for 10 minutes in 2× Laemmli sample buffer (Santa Cruz, #sc-286962). Equal protein amounts were separated on 3-8% NuPAGE Tris-Acetate gels (Invitrogen # EA0375BOX) or

(5) Dapagliflozin Mitigates Hypotension in Lipopolysaccharide-Induced Acute Inflammation Independent of Glycemia LevelPharmaceuticsJune 8, 2023Alanazi WA, Alharbi T, El-Nagar DM, Albogami AM, Alswayyed M.

(6) Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpinsCommunications biologyJanuary 12, 2022Luke CJ, Markovina S, Good M, Wight IE, Thomas BJ, Linneman JM, Lanik WE, Koroleva O, Coffman MR, Miedel MT, et al.

(7) Reduction of litter size during lactation in rats greatly influences fatty acid profiles in damsJournal of physiology and biochemistryNovember 1, 2021Amusquivar E, Sánchez-Blanco C, Herrera E.

(8) Overexpression of Neuron-Derived Orphan Receptor 1 (NOR-1) Rescues Cardiomyocytes from Cell Death and Improves Viability after Doxorubicin Induced StressBiomedicinesSeptember 16, 2021Berg PC, Hansson ÅML, Røsand Ø, Marwarha G, Høydal MA.

(9) OPN binds alpha V integrin to promote endothelial progenitor cell incorporation into vasculatureReproduction (Cambridge, England)April 1, 2020Wing TT, Erikson DW, Burghardt RC, Bazer FW, Bayless KJ, Johnson GA.

(10) A novel selenonucleoside suppresses tumor growth by targeting Skp2 degradation in paclitaxel-resistant prostate cancerBiochemical pharmacologyDecember 1, 2018Byun WS, Jin M, Yu J, Kim WK, Song J, Chung HJ, Jeong LS, Lee SK.

(11) Alterations in β‑catenin/E‑cadherin complex formation during the mechanotransduction of Saos‑2 osteoblastic cellsMolecular medicine reportsAugust 1, 2018Li FF, Zhang B, Cui JH, Chen FL, Ding Y, Feng X.washed four times with 500 ml lysis buffer and proteins were solubilized in Laemmli sample buffer (sc-286962; Santa Cruz Biotechnology, Inc.) containing 2-mercaptoethanol. Immunoprecipitated samples or samples

(12) G6PC mRNA Therapy Positively Regulates Fasting Blood Glucose and Decreases Liver Abnormalities in a Mouse Model of Glycogen Storage Disease 1aMolecular TherapyMarch 1, 2018Daniel S. Roseman, Tayeba Khan, Fabienne Rajas, Lucy S. Jun, Kirtika H. Asrani, Cleo Isaacs, Jeremiah D. Farelli, Romesh R. SubramanianTechnology). 25 μg of total protein diluted in water was denatured in 2× Laemmle buffer (Santa Cruz, sc-286962) and heated at 70°C for 10 min. After denaturation, samples were loaded on Bis-Tris 4%–12% gels (Thermo

(13) Short-Term Moderately Elevated Intraocular Pressure Is Associated With Elevated Scotopic Electroretinogram ResponsesInvestigative ophthalmology & visual scienceApril 1, 2016Choh V, Gurdita A, Tan B, Prasad RC, Bizheva K, Joos KM.Bio-Rad for Spectroscopy, Philadelphia, PA, USA) and equalized before adding 2× Laemmli Sample buffer (#SC 286962; Santa Cruz Biotechnology, Dallas, TX, USA) to each sample and loading onto 12% and 7.5% SDS-PAGE precast

(14) Rosa hybrida extract suppresses vascular smooth muscle cell responses by the targeting of signaling pathways, cell cycle regulation and matrix metalloproteinase-9 expressionInternational journal of molecular medicineApril 1, 2016Lee SJ, Won SY, Park SL, Song JH, Noh DH, Kim HM, Yin CS, Kim WJ, Moon SK.

(15) Expression, localization and possible functions of aquaporins 3 and 8 in rat digestive systemBiotechnic & histochemistry : official publication of the Biological Stain CommissionJanuary 1, 2016Zhao GX, Dong PP, Peng R, Li J, Zhang DY, Wang JY, Shen XZ, Dong L, Sun JY.

(16) Resveratrol ameliorates hepatic metaflammation and inhibits NLRP3 inflammasome activationMetabolism: clinical and experimentalMay 1, 2014Yang SJ, Lim Y.

(17) Silencing lamin A/C in human endometrial stromal cells: a model to investigate endometrial gene function and regulationMolecular human reproductionOctober 1, 2004Tulac S, Dosiou C, Suchanek E, Giudice LC.

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