Polybrene® from Santa Cruz Biotechnology, Inc.

Product Specs
ItemPolybrene®
CompanySanta Cruz Biotechnology, Inc.
Price $26.00Supplier Page View Company Product Page
Catalog Numbersc-134220
Quantity10 mg/ml
TypeMolecular biology reagents or solutions or stains
Molecular Weight374.20

Specifications/Features

Storage:
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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) GUTK induces apoptosis in reactivating quiescent prostate cancer cells via Aurora A-mediated stabilization of SOD2iScienceMay 15, 2026Yalin Wang, Yang Li, Xue Jiang, Xiaoqiong Chen, Mengfan Liu, Hangui Ren, Yulong Zhang, Rongchen Dai, Zhichao Xi, Hongxi XuRRID:SCR_014281 ,, Other,Other,Other ,, Lenti-Pac HIV Expression Packaging Kit,GeneChem,Cat# GMeasy-10 Polybrene,Santa Cruz,Cat# sc-134220

(2) Identification of Cyclin L1 as a Host Factor Regulating Hepatitis B Virus ReplicationVirusesMay 8, 2026Collins Oduor Owino, Balakrishnan Chakrapani Narmada, Gian Yi Lin, Pauline Poh Kim Aw, Nivrithi Ganesh, Jovi Tan Siying, Marie-Laure Plissonnier, Thangavelu Thangavelu Matan, Niranjan Shirgaonkar, Pablo Bifani, et al.instructions. Lentiviral transduction of the cells was carried out in the presence of 5 μg/mL polybrene (sc-134220, Santa Cruz, Dallas TX, USA), followed by selection of positive clones in the presence of 10 μg/mL puromycin

(3) 2-Hydroxyestradiol regulates extracellular matrix deposition through estrogen receptor beta activation in airway smooth muscle cellsMolecular and Cellular EndocrinologyMay 1, 2026Ashish Kumar, Mohammad Irshad Reza, Anurag Banerjee, Nilesh Sudhakar Ambhore, Premanand Balraj, Buddhadev Layek, Michael A. Thompson, John R. Hawse, Christina M. Pabelick, Y.S. Prakash, et al.freshly prepared 5% serum medium (no AbAm) and with 0.5 μl/ml polybrene (Santa Cruz Biotechnology; sc-134220). Then the Lentiviral particles were added to the cells, and the cells were incubated overnight. Post

(4) ReDisulphID: A discovery platform for thiol redox sensors identifies a druggable site regulating p53 activationRedox BiolApril 29, 2026Pierre Coleman, Anna Laddach, Rhys Anderson, Xiaoping Yang, Ravi Kumar, Ajay Shah, Franca Fraternali, Joseph R. Burgoyneserial dilution of lentivirus-containing media and supplemented with 8 μg/mL of polybrene (Santa Cruz, sc-134220) before seeding on 12-well plates. Infection media was replaced with normal media after 24 h and then

(5) Tissue-specific fibroblast lipid cues impose the rate of epithelial cancer invasionNature MetabolismApril 27, 2026Timothy Budden, Noah Palombo, Shilpa Gurung, Martha Gutteridge, Charlotte Russell, Jair Marques, Alex von Kriegsheim, Lyutong An, Catherine Harwood, Luisa Motta, et al.× 104 cells were cultured in cell culture media with 5 μg ml−1 Polybrene (Santa Cruz Biotechnology, sc-134220). Lentiviral particles were added to cells and incubated overnight. Media containing lentiviral particles

(6) Loss of Peroxiredoxin 6 Drives Age-Related Klf9/NF-κB/Nlrp3 Inflammasome Activation and Pyroptosis: Therapeutic Rescue by Prdx6Antioxidants (Basel)April 23, 2026Bhavana Chhunchha, Eri Kubo, Deepali Lehri, Dhirendra P. Singhcomplete medium; 24 h later, cells were washed and 2 mL fresh medium containing polybrene (5 µg/mL; sc-134220, Santa Cruz Biotechnology) was added to enhance transduction efficiency. LV GFP-ShControl and LV GFP-ShPrdx6

(7) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.along with polybrene at a final concentration of 4 μg/mL of polybrene (Santa Cruz Biotechnology Cat# sc‐134220). For mouse FL LSK cells and Kmt2a::AFF1+ pMIRH‐128a pro‐B leukemic blasts, the supernatant (MOI > 10)

(8) Hydrogen sulfide alleviates hyperoxia effects on mitochondria in human developing airway smooth muscleJCI InsightApril 22, 2026Colleen M. Bartman, Michael Thompson, Samantha K. Hamrick, Niyati A. Borkar, Daniel Pfeffer-Kleemann, Preetham Ravi, Marta Schiliro, Yak Nak, Christian Vivar Ramon, Li Drake, et al.approximately 60% confluence, DMEM/F12 was added to the cells with 5 mg/mL Polybrene (Santa Cruz Biotechnology, sc-134220). shRNA lentiviral particles were added to the cells and incubated overnight. A minimum of 3 different

(9) FXR and BET signaling orchestrate to protect β cellsbioRxivApril 17, 2026Fritz Cayabyab, Jalan Tipirneni, David Chen, Jinhyuk Choi, Yu Hamba, Nhi Pham, Clarissa Tacto, Jie Wu, Liu Wang, Yeva Mirzakhanyan, et al.(TAKARA, 631232) for long-term storage at −80°C in a deep freezer. 10 μg/ml Polybrene (Santa Cruz, sc-134220) was used as a scaffold for virus infection. GSIS assay Isolated pancreatic islets were pre-cultured

(10) Targeting peptide–MHC complexes with designed T cell receptors and antibodiesbioRxivApril 16, 2026Amir Motmaen, Kevin M. Jude, Nan Wang, Anastasia Minervina, David Feldman, Mauriz A. Lichtenstein, Abishai Ebenezer, Colin Correnti, Paul G. Thomas, K. Christopher Garcia, et al.resuspended in complete medium optionally supplemented with 8 μg/mL polybrene (Santa Cruz Biotechnology, sc-134220) and mixed with 5 to 200 uL of fresh or thawed lentivirus. Optionally, cells were spinned at 1000 ×

(11) Pre-assembly of biomolecular condensate seeds drives RSV replicationNatureApril 2, 2026Dhanushika Ratnayake, Marie Galloux, Sanne Boersma, Marko Noerenberg, Christina Sizun, Carlos Sacristan, Julien Sourimant, Anke J. Lakerveld, Anne T. Gelderloos, Leonie Apperloo, et al.remove cellular debris, and was added to recipient cells of interest along with 10 mg ml−1 polybrene (sc-134220, Santa Cruz Biotechnology) and subject to spin infection for 120 min at 2,000 rpm at 25 °C. Following

(12) Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2 / STAT3 Signaling PathwayCNS Neuroscience & TherapeuticsApril 1, 2026Gang Zhou, Fengjiao Hu, Ju Gao, Lei Wang, Xiangbo Wu, Yu Tian, Peng Zhang, Guangming Xia, Feng Wan, Jinhua WangMillipore). The lentivirus supernatant was used to infect HT22 cells in the presence of 10 μg/mL polybrene (SC‐134220, Santa Cruz). Transduced HT22 cells were selected with 2.5 μg/mL puromycin (BLGE320‐25MG, Biolight)

(13) STING–STAT3–SOX18 Axis Drives EndMT and Epigenetic Reprogramming in SAVI Lung FibrosisbioRxivMarch 27, 2026Dan Yang, Guibin Chen, Sachin Gaurav, Adriana A. de Jesus, Atul K. Mehta, Colton McNinch, Adam X. Miranda, Jinzhi Wei, Noemi Kedei, Maria Olga Hernandez, et al.Calcein AM,BD Biosciences,564061 RNase Inhibitor,New England Biolabs,M0307S Polybrene,Santa Cruz,sc-134220 DiI-Ac-LDL,Invitrogen,L3484 "Cyclic [G(2′,5′)pA(3′,5′)p] (2′3′cGAMP)",Invivogen,tlrl-nacga23-5 human

(14) Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeresCell ReportsMarch 24, 2026Erin R. Taylor, Bruce Proctor, Melina Vaurs, Guanhui Wu, Manon Mahieu, Shreya Shrestha, Daniel Morrison, Laila Weatherly, Shane Brelinsky, Maya Raghunandan, et al.membrane (Whatman Uniflo, #9915–2504). Viral media and 8 μg/mL polybrene (Santa Cruz Biotechnology, #sc-134220) were added to cells for infection. Culture media was changed after 24 h with 2.5 mL complete DMEM.

(15) Cystinosin regulates Na+/H+ exchanger 3 trafficking and function in kidney proximal tubular cellsEMBO ReportsMarch 24, 2026Veenita Khare, Jean-Claude Farré, Mouad Ait Kbaich, Céline J Rocca, Cynthia Tang, Xuan Ma, Kavya Biederman, Ioli Mathur, Rafael A Badell-Grau, Anusha Sivakumar, et al.NHE-3 shRNA (h) lentiviral particles (Santa cruz, sc-36059-V). Briefly, after one day of culturing the cells, polybrene (sc-134220) was added to the culture medium at a final concentration of 5 µg/ml, followed by the addition of viral

(16) Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomicsSTAR ProtocMarch 20, 2026Hannah Thomas, Garry Dolton, Lucia F. Cardo, Nicola Ternette, Andrew K. Sewell, Thierry SchmidlinF7524 Penicillin-Streptomycin,Merck,Cat# P4333 L-glutamine,Merck,Cat# G7513 Hexadimethrine bromide (polybrene®),Santa Cruz,Cat# sc-134220 Roswell Park Memorial Institute 1640 medium (RPMI 1640),Merck,Cat# R8759 Dulbecco’s Phosphate Buffered Saline

(17) KLF5 controls subtype-independent highly interactive enhancers in pancreatic cancer to regulate cell survivalSci AdvMarch 20, 2026Thomas L. Ekstrom, Zhangshuai Dai, Julia Thiel, Akshay Kanakan, Bishakha Joyeeta Saha, Meghana Manjunath, Nadine Schacherer, Pavlos Bousounis, Emily L Siegler, Amro M. Abdelrahman, et al.plate were infected by adding a final concentration of polybrene (8 μg/ml; Santa Cruz Biotechnology, sc-134220), adding 100 μl of the concentrated virus, and centrifuging for 1000g for 2 hours at 30°C. Infected

(18) Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloidsSTAR ProtocolsMarch 20, 2026Giulia Perticari, Maroussia M.P. Ganpat, Jarno Drost25000, Transfection Grade (PEI 25K)",Tebu-Bio,Cat# 23966-100 Polybrene,Santa Cruz Biotechnology,Cat# SC-134220 Paraformaldehyde 16% (w/v) in aqueous solution methanol-free,VWR,Cat# 43368.9M Butanol,Avantor,Cat#

(19) The E3 ubiquitin ligase mechanism specifying targeted microRNA degradationNatureMarch 18, 2026Jakob Farnung, Elena Slobodyanyuk, Peter Y. Wang, Lianne W. Blodgett, Daniel H. Lin, Susanne von Gronau, Brenda A. Schulman, David P. Bartel40% of the total) was combined with 1 ml of media and 12 μg ml−1 polybrene (Santa Cruz Biotechnology, SC-134220), and then added to one well of a six-well plate containing MEFs that were plated 24 h before at around

(20) Control of Inflammatory Response by Tissue MicroenvironmentbioRxivMarch 18, 2026Zhongyang Wu, Scott D. Pope, Nasiha S. Ahmed, Diana L. Leung, Stephanie Hajjar, Qiuyu Yue, Diya M. Anand, Elizabeth B. Kopp, Daniel Okin, Weiyi Ma, et al.L4524 Nigericin,Invitrogen,Cat# N1495 optiMEM reduced serum media,Gibco,Cat# 31985-062 polybrene,Santa Cruz,Cat# sc-134220 Matrigel Matrix,Corning,Cat# 356231 Hoechst 33342 solution,Thermo Scientific,Cat# 62249 Hydrochloric acid solution

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Polybrene®

Polybrene® from Santa Cruz Biotechnology, Inc.

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Specifications/Features

Santa Cruz Biotechnology, Inc.

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Polybrene® from Santa Cruz Biotechnology, Inc.

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