Red Blood Cell (RBC) Lysis Buffer (10x) from Abcam

Product Specs
ItemRed Blood Cell (RBC) Lysis Buffer (10x)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204733
Quantity100 mL
Sample TypeTissue, Whole Blood

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) LINC02470 impairs natural killer cell cytotoxicity by epigenetically targeting the natural cytotoxicity triggering receptor 1Front ImmunolApril 16, 2026Xiumin Shi, Lei Zhou, Min Li, Xue Wen, Yongchong Chen, Chao Niu, Qiliang Yin, Haofan Jin, Andrew R. Hoffman, Jiuwei Cui, et al.Argentina). The bone marrow from healthy donor was treated with erythrocyte lysis buffer (Abcam, catalog ab204733). After mix and subsequent centrifugation, the resulting cell pellet was collected. Peripheral blood

(2) Therapeutic effects of IL-33/ST-2 pathway inhibition combined with albendazole on hepatic fibrosis and immune regulation in alveolar echinococcosis: in vivo and in vitro evidenceParasit VectorsApril 3, 2026Shi-Lei Cheng, Xiu-Mei Ma, Bin-Jie Wu, Yu-Xuan Yang, Hai-Ning Fansubpopulation within the CD45+ gate. The reagents used included 10 × red blood cell (RBC) lysis buffer (ab204733, Abcam, Cambridge, UK) and an intracellular fixation and permeabilization buffer set (product no. eBioscience-88-8824-00,

(3) A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channelsJournal of Biological ChemistryFebruary 1, 2026Fabian M.B. Thöne, Maya M. Polovitskaya, Uta E. Höpken, Armin Rehm, Thomas J. JentschErythrocyte lysis was performed for blood and spleens for 2 min using red blood cell lysis buffer (Abcam, ab204733). Single-cell suspensions were either used immediately (investigation of immune cells in naive Lrrc8b−/−

(4) zAvatar-test—A functional precision model to personalize ovarian cancer treatments: Results from a co-clinical studyCell Reports MedicineJanuary 20, 2026Marta F. Estrada, Filipa Amorim, Filipa Ferreira da Silva, Cátia Rebelo de Almeida, Márcia Fontes, Ricardo Coelho, Sónia Ferreira, Rita Canas-Marques, Mireia Castillo-Martin, João Casanova, et al.at room temperature. The cell pellet was resuspended in 1x red blood cell lysis buffer (Abcam, ref ab204733) to remove erythrocytes. The cell pellets were then cryopreserved in 90% (v/v) fetal bovine serum (FBS;

(5) Single-cell and spatial transcriptomic profiling reveals distinct tumor microenvironment dynamics in cervical adenocarcinoma and squamous cell carcinomaCommunications BiologyDecember 19, 2025Yaqin Wu, Bei Wei, Zhentong Wei, Hongyan Wang, Yunyun Liu, Dongdong Xu, Xiaokang Li, Liyuan Guo, Huaiwu Lu, Huiling Shangmedium for three times. The cell pellet was resuspended with 1x chilled Red Blood Cell Lysis Buffer (ab204733, Abcam) and incubated on ice for 10 min, and then washed with cold PBS three times. The single cell

(6) LXR pathway drives hormonal response intensity in polycystic ovary syndromeEMBO Molecular MedicineMay 21, 2025Sarah Dallel, Manon Despalles, Margaux Tore, Yoan Renaud, Ayhan Kocer, Christelle Damon-Soubeyrand, Pierre Pouchin, Caroline Vachias, Katia Boutourlinsky, Céline Gonthier-Gueret, et al.success by flow cytometry using red blood lysis buffer according manufacturer instructions (Abcam, Ab204733). After blocking with anti-CD16/CD32 (Pharmigen 553141), samples were analyzed using Live/Dead fixable

(7) Endosomal chloride/proton exchangers need inhibitory TMEM9 β-subunits for regulation and prevention of disease-causing overactivityNature communicationsApril 1, 2025Planells-Cases R, Vorobeva V, Kar S, Schmitt FW, Schulte U, Schrecker M, Hite RK, Fakler B, Jentsch TJ.sexes. Cells were flushed from bone, and erythrocytes lysed in red blood cell lysis buffer (Abcam, ab204733). Remaining cells were resuspended in complete DMEM medium. Four hours after seeding, non-attached cells

(8) Engineering exosome membrane disguised thermal responsive system for targeted drug delivery and controlled release across the blood-brain barrierMater Today BioMarch 11, 2025Zhe Han, Haina Huang, Boyan Li, RongRong Zhao, Qingtong Wang, Hong Liu, Hao Xue, Weijia Zhou, Gang Licentrifuged at 300×g for 5 min. The resulting precipitate was resuspended in 0.8 ml of 1 × RBC Lysis Buffer (ab204733, Abcam) using a 1000 μL pipette with continuous shaking for 20–30 s. The reaction was terminated by

(9) EpiAge: a next-generation sequencing-based ELOVL2 epigenetic clock for biological age assessment in saliva and blood across health and diseaseAging (Albany NY)January 22, 2025David Cheishvili, Sonia Do Do Carmo, Filippo Caraci, Margherita Grasso, A Claudio Cuello, Moshe Szyfsterile centrifuge tube. After a washing step by using 5 ml of 1X Red Blood Cell Lysis Buffer (Abcam, ab204733) for optimal lysis of erythrocytes in a single-cell suspension and after two additional washing steps

(10) A novel approach to engineering three-dimensional bladder tumor models for drug testingScientific reportsNovember 6, 2024Monjaras-Avila CU, Luque-Badillo AC, Bacon JVM, Wyatt AW, So A, Chavez-Munoz C.were washed once with 1X PBS and incubated for 5 min with 1X Red Blood Cell (RBC) Lysis Buffer (ABCam, ab204733). The cellular pellet was resuspended in a 1:1:1ratio mixture of REBM (Lonza, CC-3190), EBM-2 (Lonza,

(11) Ganglion cell-derived LysoPS induces retinal neovascularisation by activating the microglial GPR34-PI3K-AKT-NINJ1 axisJournal of neuroinflammationOctober 28, 2024Chen L, Zhang H, Zhang Y, Li X, Wang M, Shen Y, Cao Y, Xu Y, Yao J.rpm for 5 min to remove debris. After removing the supernatants, 2 ml red blood cell lysis buffer (ab204733, Abcam) was added to the cell pellet for 5 min to lyse the erythrocytes, followed by centrifugation

(12) Transgenic Targeting of Fcrls Creates a Highly Efficient Constitutively Active Microglia Cre Line with Differentiated SpecificityeNeuroJune 26, 2024Tobias Kaiser, Jordan Dattero, Liang Li, Mandy Chen, Minqing Jiang, Andrew Harrahill, Oleg Butovsky, Guoping Fengof 10% (w/v) EDTA. The 2 ml of whole blood was added to 40 ml of red blood cell lysis buffer (Abcam, ab204733) and incubated for 10 min at room temperature to lyse red blood cells. The suspension containing lysed

(13) Low TGF-β1 plasma levels are associated with cognitive decline in Down syndromeFrontiers in pharmacologyJanuary 1, 2024Grasso M, Fidilio A, L'Episcopo F, Recupero M, Barone C, Bacalini MG, Benatti C, Giambirtone MC, Caruso G, Greco D, et al.to a new sterile centrifuge tube. After washing using 5 mL of 1× Red Blood Cell Lysis Buffer (Abcam, ab204733) for optimal lysis of erythrocytes in a single-cell suspension and after two additional washing steps

(14) Neutrophil-derived Activin-A moderates their pro-NETotic activity and attenuates collateral tissue damage caused by Influenza A virus infectionFrontiers in immunologyJanuary 1, 2024Divolis G, Synolaki E, Doulou A, Gavriil A, Giannouli CC, Apostolidou A, Foster ML, Matzuk MM, Skendros P, Galani IE, et al.washed in ice-cold PBS, and red blood cell (RBC) lysis was performed with the RBC lysis buffer (Abcam, ab204733), according to the manufacturer’s instructions. Then, cells were washed with 10 ml ice-cold PBS supplemented

(15) Effect of erythrophagocytosis-induced ferroptosis during angiogenesis in atherosclerotic plaquesAngiogenesisNovember 1, 2023Puylaert P, Roth L, Van Praet M, Pintelon I, Dumitrascu C, van Nuijs A, Klejborowska G, Guns PJ, Berghe TV, Augustyns K, et al.or attached to (though not internalized by) BMDMs were removed with Erythrocyte Lysis Buffer (Abcam, ab204733). Finally, BMDMs were detached with 0.25% trypsin-EDTA (Thermo Fischer Scientific, 25200072) and analyzed

(16) Drug-Loaded Lipid Magnetic Nanoparticles for Combined Local Hyperthermia and Chemotherapy against Glioblastoma MultiformeACS NanoSeptember 26, 2023Lilianne Beola, Nerea Iturrioz-Rodríguez, Carlotta Pucci, Rosalia Bertorelli, Gianni Ciofanicentrifugation at 300g for 5 min. The resulting pellet was resuspended in 0.8 mL of 1× RBC Lysis Buffer (ab204733, Abcam) by continuous shaking with a 1000 μL pipet for 20–30 s. The reaction was stopped by adding DMEM

(17) Diversity of B Cell Populations and Immunoglobulin Repertoire in Human LungsThe Journal of ImmunologyAugust 1, 2023Fumiaki Aihara, Yumei Wang, Anna C. Belkina, Rachel Fearns, Joseph P. Mizgerd, Feng Feng, Thomas B. Keplersample was spun down, the supernatant removed, and then treated with red blood cell lysis buffer (Abcam ab204733). The final product was resuspended in PBS (Sigma RNBK1816) for staining. PBMC Isolation The bottom

(18) The pharmacokinetic profile of brensocatib and its effect on pharmacodynamic biomarkers including NE, PR3, and CatG in various rodent speciesFrontiers in PharmacologyJuly 19, 2023Jessica Basso, Kuan-Ju Chen, Yuchen Zhou, Lilly Mark, Daniel LaSala, Arielle Dorfman, Mary Atalla, Donald Chun, Veronica Viramontes, Christina Chang, et al.aspirated. After which, bone marrow cells were lysed with 1X red blood cell (RBC) lysis buffer (Abcam, ab204733) to eliminate contaminating RBCs and washed with PBS to remove any residual RBC lysis buffer. The resulting

(19) Validation of a Novel EUS-FNB-Derived Organoid Co-Culture System for Drug Screening in Patients with Pancreatic CancerCancers (Basel)July 19, 2023Simon Ezban Grützmeier, Bojan Kovacevic, Peter Vilmann, Charlotte Vestrup Rift, Linea Cecilie Melchior, Morten Orebo Holmström, Lene Brink, Hazem Hassan, John Gásdal Karstensen, Hanne Grossjohann, et al.single cells was avoided to preserve tumor cells and CAF viability. Use of red blood cell lysis buffer (ab204733, Abcam, Cambridge, UK) was applied if needed. Cells were resuspended in growth-factor-reduced Matrigel®

(20) ClC-7 drives intraphagosomal chloride accumulation to support hydrolase activity and phagosome resolutionThe Journal of cell biologyJune 5, 2023Wu JZ, Zeziulia M, Kwon W, Jentsch TJ, Grinstein S, Freeman SA.cells were flushed out of bones with PBS, erythrocytes were lysed with red blood cell lysis buffer (ab204733; Abcam), and cells were resuspended in complete DMEM supplemented with 10% FBS, 1% penicillin–streptomycin,

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Red Blood Cell (RBC) Lysis Buffer (10x) from Abcam

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Red Blood Cell (RBC) Lysis Buffer (10x) from Abcam

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