Description
Designed to maximize fluorescence signal intensities generated by pH-sensitive fluorochromes. In addition, this buffer contains a metabolic inhibitor sodium azide (NaN3) and animal serum proteins to help minimize non-specific binding. NaN3 helps inhibit patching and capping of cell surface antigens. It also inhibits the potential redistribution of cell surface antigens caused by antibody cross linking, thereby preventing the potential loss of fluorescent signal intensities generated by immunofluorescently-stained cells during subsequent flow cytometric analysis