SignalFire ™ Plus ECL Reagent from Cell Signaling Technology

Product Specs
ItemSignalFire ™ Plus ECL Reagent
CompanyCell Signaling Technology
Price $117.00 Inquire
Catalog Number12630P
Quantity20 ml
ApplicationsW
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Sex Disparity in Systemic Sclerosis-Associated Pulmonary FibrosisInt J Mol SciMay 14, 2026Audrey N. Galimba, Ludivine Renaud, Samantha E. Kotz, Erica L. Herzog, Carol Feghali-BostwickWest Pico (Cat# 34578, ThermoFisher Scientific, Waltham, MA, USA) or SignalFire Plus ECL reagent (Cat# 12630S, Cell Signaling, Danvers, MA, USA) and imaged on the iBright750 system (ThermoFisher Scientific, Waltham,

(2) Degradation of the TEAD•YAP/TAZ Transcription Factor Complex by Heterobifunctional Small Molecules that Bind to the TEAD Allosteric Lipid PocketbioRxivJanuary 14, 2026I-Ju Yeh, Mona K. Ghozayel, Khuchtumur Bum-Erdene, Samy O. Merouehinhibitor,Fisher,Cat# A32957 Protease inhibitor,Fisher,Cat# A32955 ECL substrate,Cell Signaling,Cat# 12630S "Anti-rabbit IgG, HRP-linked Antibody",Cell Signaling,Cat# 7074S "Anti-mouse IgG, HRP-linked Antibody",Cell Signaling

(3) MUC4 mutations promote a thrombotic phenotype in patients with paroxysmal nocturnal haemoglobinuria by increasing the deposition of terminal complementClin Transl MedJanuary 5, 2026Yingying Chen, Mengting Che, Chaomeng Wang, Qiaoyi Bronte Zhang, Weixin Chen, Hui Liu, Chunyan Liu, Guang Sheng Ling, Rong Fufollowed by HRP‐conjugated secondary antibodies (7074/7076; CST). Signals were detected using ECL reagent (12630; CST) and a GelView6000Plus Smart Chemiluminescence Imaging System (Guangzhou Biolight Biotechnology

(4) Enhanced SLC2A4 expression mitigates insulin resistance in gestational diabetes mellitus via the FoxO signaling pathway in vitroExperimental and Therapeutic MedicineAugust 22, 2025Yanqi Jin, Xiaoyan Cheng, Jinting Wu, Yi Zhu, Hui Xu, Chunyu Caoh at room temperature. Protein bands were visualized using a SignalFire Plus ECL reagent (cat. no. 12630S; Cell Signaling Technology, Inc.), with GAPDH serving as an internal reference. Protein expression levels

(5) Contribution of Sorting Nexin 3 in the Cytomegalovirus AssemblyBiomedicinesApril 11, 2025Ivona Viduka, Igor Štimac, Silvija Lukanović Jurić, Tamara Gulić, Berislav Lisnić, Gordana Blagojević Zagorac, Hana Mahmutefendić Lučin, Pero LučinSignalFire [TM] Elite ECL Reagent; Cell Signaling, Cell Signaling Inc., Danvers, MA, USA; Cat. No. 12630S or 12757P, respectively) using ImageQuant LAS 500 (GE Healthcare Bio-Sciences AB, Upsala, Sweden). The

(6) miR-138-5p inhibits healing of femoral fracture osteogenesis in rats by modulating osteoblast differentiation via SIRT1/FOXO1 axisJ Orthop Surg ResMarch 14, 2025Guangming Dai, Wei Jiang, Bo Feng, Lan ZhangCell Signaling Technology) for an hour at 25 °C. The blots were visualized by treated with ECL reagents (12630P, Cell Signaling Technology) and then photographed applying the Amersham Imager 600 imagers (GE, USA).

(7) Rab10-associated tubulation as an early marker for biogenesis of the assembly compartment in cytomegalovirus-infected cellsFront Cell Dev BiolJanuary 10, 2025Hana Mahmutefendić Lučin, Igor Štimac, Marina Marcelić, Matej Skočaj, Berislav Lisnić, Alen Omerović, Ivona Viduka, Barbara Radić, Ljerka Karleuša, Gordana Blagojević Zagorac, et al.chemiluminescence (SignalFire [TM] Plus ECL Reagent or SignalFire [TM] Elite ECL Reagent; Cell Signaling, Cat. No. 12630S and 12757P, respectively) using Transilluminator Alliance 4.7 (Uvitec Ltd., Cambridge, United Kingdom)

(8) SNX27:Retromer:ESCPE-1-mediated early endosomal tubulation impacts cytomegalovirus replicationFrontiers in Cellular and Infection MicrobiologySeptember 18, 2024Igor Štimac, Marina Marcelić, Barbara Radić, Ivona Viduka, Gordana Blagojević Zagorac, Silvija Lukanović Jurić, Carmen Rožmanić, Martin Messerle, Ilija Brizić, Pero Lučin, et al.chemiluminescence (SignalFire [TM] Plus ECL Reagent or SignalFire [TM] Elite ECL Reagent; Cell Signaling, Cat. No. 12630S or 12757P, respectively) using Transilluminator Alliance 4.7 (Uvitec Ltd., Cambridge, UK) and ImageQuant LAS 500

(9) IGF-II regulates lysyl oxidase propeptide and mediates its effects in part via basic helix-loop-helix E40Matrix BiologySeptember 1, 2024Adegboyega Timothy Adewale, Shailza Sharma, Joe E. Mouawad, Xinh-Xinh Nguyen, Amy D. Bradshaw, Carol Feghali-BostwickSubstrate (Cat. # 34580, Thermo Scientific, Waltham, MA) and SignalFire™ Plus ECL detection reagent (Cat. # 12630, Cell Signaling, Danvers, MA) on the iBright™ Imaging System (Thermo Scientific). ImageJ software (Version

(10) Age-dependent effects of metformin on human oligodendrocyte lineage cell ensheathment capacityBrain communicationsJanuary 1, 2024Mohammadnia A, Cui QL, Weng C, Yaqubi M, Fernandes MGF, Hall JA, Dudley R, Srour M, Kennedy TE, Stratton JA, et al.peroxidase-conjugated secondary antibody used in conjunction with an ECL Western blot detection kit (Cell Signalling #12630S). The blots were quantified with the Gels program in ImageJ, and the value of the pAMPK was corrected

(11) MiR-182-5p: A Novel Biomarker in the Treatment of Depression in CSDS-Induced MiceInternational Journal of NeuropsychopharmacologyJanuary 1, 2024Ya-Bin Zheng, Xiao-Ming Sheng, Xiang Jin, Wei Guan7076S) at room temperature. The membrane was detected using enhanced chemiluminescence (Cell Signaling; 12630) reagent (Cai et al., 2021; Li et al., 2022). Statistical Analysis The statistical analyses and bar

(12) Phoenixin-20 ameliorates Sevoflurane inhalation-induced post-operative cognitive dysfunction in rats via activation of the PKA/CREB signalingAgingDecember 4, 2023Zhang J, Wang Z, Cong K, Qi J, Sun L.secondary antibody (Cat#7074 or Cat#7076, 1:5000, CST, USA) and then the ECL luminescence solution (Cat#12630, CST, USA) was introduced for exposure. The image J system was used to analyze the gray value of protein

(13) K+/Cl− cotransporter 2 (KCC2) and Na+/HCO3− cotransporter 1 (NBCe1) interaction modulates profile of KCC2 phosphorylationFrontiers in Cellular NeuroscienceOctober 10, 2023Abhishek Pethe, Mira Hamze, Marina Giannaki, Bernd Heimrich, Igor Medina, Anna-Maria Hartmann, Eleni Roussawere developed in enhanced chemiluminescence reagents (Signalfire ECL reagent, Cell Signaling Cat# 12630S), and signals were visualized on X-ray films (Amersham Hyperfilm ECL, Cytiva Cat# 28906837). 2.8. Immunocytochemistry HEK-293 cells

(14) The Role of SOX9 in IGF-II-Mediated Pulmonary FibrosisInternational journal of molecular sciencesJuly 8, 2023Waldrep KM, Rodgers JI, Garrett SM, Wolf BJ, Feghali-Bostwick CA.antibodies for 1 h at room temperature, washed with TBST, and imaged. The SignalFire Plus ECL reagent (Cat#12630S, Cell Signaling, Danvers, MA, USA) and the SuperSignal West Pico Plus reagent (Cat# 34578, Thermo Fisher Scientific

(15) ARPC5 deficiency leads to severe early-onset systemic inflammation and mortalityDisease models & mechanismsJuly 1, 2023Sindram E, Caballero-Oteyza A, Kogata N, Chor Mei Huang S, Alizadeh Z, Gámez-Díaz L, Fazlollhi MR, Peng X, Grimbacher B, Way M, et al.secondary antibodies before detection with ECL chemiluminescent substrate (Cell Signaling Technology, #12630S, 1:1). Anti-Tubulin (Proteintech, #HRP-66031, 1:5000) was used as a loading control and detected as

(16) The Potent G-Quadruplex-Binding Compound QN-302 Downregulates S100P Gene Expression in Cells and in an In Vivo Model of Pancreatic CancerMoleculesMarch 7, 2023Ahmed A. Ahmed, William Greenhalf, Daniel H. Palmer, Nicole Williams, Jenny Worthington, Tariq Arshad, Shozeb Haider, Effrosyni Alexandrou, Dilek Guneri, Zoe A. E. Waller, et al.Following washes in TBS-T, bands were observed following addition of SignalFire Plus ECL reagent (CST#12630). Images were taken using a GeneGnome imaging system (Syngene, UK). Densiometric analysis of the bands

(17) Temozolomide, Simvastatin and Acetylshikonin Combination Induces Mitochondrial-Dependent Apoptosis in GBM Cells, Which Is Regulated by AutophagyBiology (Basel)February 14, 2023Sima Hajiahmadi, Shahrokh Lorzadeh, Rosa Iranpour, Saeed Karima, Masoumeh Rajabibazl, Zahra Shahsavari, Saeid Ghavamifrom Sigma-Aldrich (Oakville, ON, Canada) as well. The enhanced chemiluminescence (ECL) (CAS Number: 12630) was acquired from Cell Signaling Technology Co. (Beverly, MA, USA). The bicinchoninic acid (BCA) protein

(18) First Characterization of the Transcriptome of Lung Fibroblasts of SSc Patients and Healthy Donors of African AncestryInternational Journal of Molecular SciencesFebruary 11, 2023Ludivine Renaud, Kristy M. Waldrep, Willian A. da Silveira, Joseph M. Pilewski, Carol A. Feghali-Bostwickdilutions for one hour at room temperature, washed again, and imaged using SignalFire Plus ECL reagent (Cat#12630S, Cell Signaling, Danvers, MA, USA) or SuperSignal West Pico Plus (Cat# 34578, ThermoFisher Scientific,

(19) An improved cell line-derived xenograft humanized mouse model for evaluation of PD-1/PD-L1 blocker BMS202-induced immune responses in colorectal cancerActa biochimica et biophysica SinicaOctober 25, 2022Shang P, Yu L, Cao S, Guo C, Zhang W.Cell Signaling Technology, Danvers, USA). These blots were detected using SignaIFire TM Plus ECL Reagent (12630; Cell Signaling Technology, Danvers, USA) and analyzed using the Tianon infrared imaging system and

(20) LDL receptor-related protein 1 (LRP1), a novel target for opening the blood-labyrinth barrier (BLB)Signal transduction and targeted therapyJune 10, 2022Shi X, Wang Z, Ren W, Chen L, Xu C, Li M, Fan S, Xu Y, Chen M, Zheng F, et al.milk diluted with TBST (1× working solution). The protein bands were visualized with an ECL Plus (CST, 12630P) chemiluminescence detection system (GE Healthcare). The exposure time period was 30 s, and the signal

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SignalFire ™ Plus ECL Reagent from Cell Signaling Technology

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SignalFire ™ Plus ECL Reagent from Cell Signaling Technology

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