DRAQ5(TM) from BioLegend

Product Specs
ItemDRAQ5(TM)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number424101
Quantity50 µL
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-F, Immunohistochemistry (IHC)-P
TargetDRAQ5

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Dissecting the influence of cellular senescence on cell mechanics and extracellular matrix formation in vitroAging CellMarch 1, 2023Erik Brauer, Tobias Lange, Daniela Keller, Sophie Görlitz, Simone Cho, Jacqueline Keye, Manfred Gossen, Ansgar Petersen, Uwe KornakFischer). F‐actin and cell nuclei were visualized using SYTOX™ Green (#S7020, Thermo Fischer), Draq5 (#424101, Biolegend) and Phalloidin‐Atto550 (19083, Sigma Aldrich). 4.18 SEM Fixed samples were incubated 2

(2) Redox-Dependent Modulation of Human Liver Progenitor Cell Line FateInternational Journal of Molecular SciencesJanuary 18, 2023Francesco Bellanti, Domenica Mangieri, Giorgia di Bello, Aurelio Lo Buglio, Giuseppe Pannone, Maria Carmela Pedicillo, Alberto Fersini, Michał Dobrakowski, Aleksandra Kasperczyk, Sławomir Kasperczyk, et al.−20 °C. The following day, cells were centrifuged at 300× g for 3 min and stained with 5 μM DRAQ5 (424101, BioLegend, San Diego, CA, USA) for 15 min at room temperature. To study cellular phenotype, PE-labeled

(3) In vivo labeling reveals continuous trafficking of TCF-1+ T cells between tumor and lymphoid tissueJournal of Experimental MedicineJune 6, 2022Zhi Li et al.Bright), fixed with BD Cytofix fixation buffer, and then stained with a far red DNA dye Draq5 (1:1,000, 424101; BioLegend). Images were taken using a Zeiss LSM 880 microscope (Zeiss) and analyzed with ImageJ. Bulk

(4) A multi-organ chip with matured tissue niches linked by vascular flowNature biomedical engineeringApril 1, 2022Ronaldson-Bouchard K, Teles D, Yeager K, Tavakol DN, Zhao Y, Chramiec A, Tagore S, Summers M, Stylianos S, Tamargo M, et al.Biolegend, 301324), PE anti-human CD68 Antibody (1:100, Biolegend, 333807), DRAQ5 (1:100, Biolegend, 424101), BUV395 Mouse Anti-Human ITGAX (CD11b) (1:100, BD Biosciences, 563787). Cells were measured using a

(5) Endothelial deletion of the cytochrome P450 reductase leads to cardiac remodellingFrontiers in physiologyJanuary 1, 2022Lopez M, Malacarne PF, Ramanujam DP, Warwick T, Müller N, Hu J, Dewenter M, Weigert A, Günther S, Gilsbach R, et al.were resuspended in FACS buffer 1 (DPBS, 0.5% BSA and 2 mM EDTA, 200 µl) containing DRAQ5 (1:200, #424101, BioLegend, Amsterdam, Netherlands). Cells positive for both DRAQ5 and Lectin were selected and FACS-sorted

(6) Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiationnpj Regenerative MedicineDecember 1, 2021Francesco Bellanti et al.The day after, cells were centrifuged at 300 × g for 3 min and stained with 5 μM DRAQ5 (BioLegend, 424101) for 15 min, at RT. Mitochondrial membrane potential was analyzed using JC-10 (Abcam, ab112133) as a

(7) NAA80 bi-allelic missense variants result in high-frequency hearing loss, muscle weakness and developmental delayBrain CommunicationsOctober 26, 2021Irena J J Muffels, Elsa Wiame, Sabine A Fuchs, Maarten P G Massink, Holger Rehmann, Jiska L I Musch, Gijs Van Haaften, Didier Vertommen, Emile van Schaftingen, Peter M van Hasseltafter fixation and permeabilization, they were stained with 1:100 phalloidin-TRITC and 1:8000 DRAQ5 (#424101, Biolegend, San Diego, USA). Cells were analysed with BD LSR Fortessa (BD Biosciences, San Jose, USA).

(8) In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic toolPlateletsOctober 3, 2021Butov KR, Osipova EY, Mikhalkin NB, Trubina NM, Panteleev MA, Machlus KR.Human recombinant SCF (Stem cell factor) – Stemcell Catalog # 78062.1 Optional: DRAQ5™ – Biolegend Catalog # 424101 Helix NP™ Blue – Biolegend Catalog # 425305 Anti-CD41a antibody (HIP8 clone) conjugated with FITC

(9) ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoidsCellAugust 19, 2021Bowles KR, Silva MC, Whitney K, Bertucci T, Berlind JE, Lai JD, Garza JC, Boles NC, Mahali S, Strang KH, et al.ImageJ software (NIH). Live cell staining was performed by incubation with 1:500 DRAQ5 (Biolegend, 424101) for 15 minutes at room temperature, per manufacturer instructions. Detection of live neurons was assessed

(10) Renal neoplasms in tuberous sclerosis mice are neurocristopathiesiScienceJuly 23, 2021Unachukwu U, Shiomi T, Goldklang M, Chada K, D'Armiento J.strainer, and resuspended in PBS containing 2% FBS, DNase I for cell sorting, and DRAQ5 (1:1000, Cat# 424101, BioLegend, San Diego, CA) in some experiments to distinguish viable cells. Flow cytometry was performed

(11) Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shorteningCell reportsMay 11, 2021Tichy ED, Ma N, Sidibe D, Loro E, Kocan J, Chen DZ, Khurana TS, Hasty P, Mourkioti F.C8267 Cytoseal-Xyl,Thermo Scientific,Cat# 8312-4 DAPI,Sigma-Aldrich,Cat# D9542 DRAQ5,Biolegend,Cat# 424101 Dextran sulfate sodium salt,Sigma-Aldrich,Cat# D8906 Dispase II,Roche,Cat# 04942078001 DMEM high

(12) Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sortingSTAR protocolsMarch 19, 2021Nicolás-Ávila JÁ, Sánchez-Diaz M, Hidalgo A.and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Draq5,BioLegend,CAT# 424101 DAPI,Thermo Fisher,CAT# D3571 HBSS,Thermo Fisher,CAT# 14175-053 DNase I,Sigma,CAT# D4527-10KU FBS,Gibco,CAT#

(13) Novel genetic features of human and mouse Purkinje cell differentiation defined by comparative transcriptomicsProceedings of the National Academy of Sciences of the United States of AmericaJune 30, 2020Buchholz DE, Carroll TS, Kocabas A, Zhu X, Behesti H, Faust PL, Stalbow L, Fang Y, Hatten ME.Dead Cell Stain was left out of the dissociation, and fixed cells were labeled with DRAQ5 (Biolegend; 424101) at 1:500 for 10 min at room temperature in the dark to label nuclei. Day 24 cells were analyzed on

(14) Cell release during perfusion reflects cold ischemic injury in rat liversScientific reportsJanuary 24, 2020de Vries RJ, Pendexter CA, Cronin SEJ, Marques B, Hafiz EOA, Muzikansky A, van Gulik TM, Markmann JF, Stott SL, Yeh H, et al.flowcytometry panels as follows. All aliquots were stained with DRAQ (1:200; Biolegend, Deadham, MA, USA; Cat# 424101) and PECy7-conjugated CD45 antibody (1:50; Biolegend, Cat# 202214). The aliquot for panel 1 was additionally

(15) B-Cell-Targeted 3DNA Nanotherapy Against Indoleamine 2,3-Dioxygenase 2 (IDO2) Ameliorates Autoimmune Arthritis in a Preclinical ModelClinical pathology (Thousand Oaks, Ventura County, Calif.)January 1, 2020Merlo LM, Bowers J, Stefanoni T, Getts R, Mandik-Nayak L.were fixed (IC Fixation Buffer, P/N 88882388; eBioscience) and subsequently incubated with DRAQ5 (P/N 424101; BioLegend) for nuclear visualization; 20 000 labeled cells were acquired using an Amnis ImageStreamX

(16) Delayed microglial depletion after spinal cord injury reduces chronic inflammation and neurodegeneration in the brain and improves neurological recovery in male miceTheranosticsJanuary 1, 2020Li Y, Ritzel RM, Khan N, Cao T, He J, Lei Z, Matyas JJ, Sabirzhanov B, Liu S, Li H, et al.on Zombie Aqua exclusion (SSC-A vs Zombie Aqua-Bv510). The cell permeant nuclear stain Draq5 (Cat# 424101, 1:500; Biolegend) was used in reference CNS samples to help delineate the leukocyte and singlet gates.

(17) Cx3cr1-deficient microglia exhibit a premature aging transcriptomeLife science allianceDecember 1, 2019Gyoneva S, Hosur R, Gosselin D, Zhang B, Ouyang Z, Cotleur AC, Peterson M, Allaire N, Challa R, Cullen P, et al.DRAQ7. The final sort gate selected CD11b+CD45lo events. Post-sort purity analysis included DRAQ5 (424101; BioLegend) for evaluating nucleated events; microglial purity was generally over 90% of nucleated events.

(18) Identification of Eph receptor signaling as a regulator of autophagy and a therapeutic target in colorectal carcinomaMolecular OncologyNovember 1, 2019Michael DiPrima et al.stained with 50 μg Hoechst 33342 (ThermoFisher; H3570) or 25 μm DRAQ5 (BioLegend, San Diego, CA, USA; 424101) for 60 min and 2 μg propidium iodide (ThermoFisher; P3566) for 15 min; analysis was performed on a

(19) Oxidative stress-mediated TXNIP loss causes RPE dysfunctionExperimental & molecular medicineOctober 15, 2019Ji Cho M, Yoon SJ, Kim W, Park J, Lee J, Park JG, Cho YL, Hun Kim J, Jang H, Park YJ, et al.× 105 cells/well) were detached and resuspended in PBS. The cells were stained with 5 μM Draq5TM (#424101, BioLegend, USA) for 15 min at room temperature. After incubation with Draq5TM, the cells were spun

(20) Synthesis and pharmacological characterization of ethylenediamine synthetic opioids in human μ-opiate receptor 1 (OPRM1) expressing cellsPharmacology research & perspectivesOctober 1, 2019Hsu T, Mallareddy JR, Yoshida K, Bustamante V, Lee T, Krstenansky JL, Zambon AC.by goat anti‐rabbit Alexa Fluor 488 (Thermo Fisher A11070) and counterstained with Draq5 (Biolegend 424101) at 1:200 dilution. Slides were mounted and visualized using Leica DMI6000 confocal microscope using

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4/25/2018Use Of DRAQ5 To Label Nuclei In Human T Cells Prior To Amnis Straining Analysis Flor NavarroDRAQ5 nuclear dye was used to label cell nuclei in order to use that as a marker of nuclei co-localization with other proteins.

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DRAQ5(TM) from BioLegend

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