Use Of DRAQ5 To Label Nuclei In Human T Cells Prior To Amnis Straining Analysis

Loyola University Chicago
Biochemistry and Molecular Biology
PhD Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

DRAQ5

Catalog Number:

424101

DRAQ5 nuclear dye was used to label cell nuclei in order to use that as a marker of nuclei co-localization with other proteins.

Experimental Design and Results Summary

Application

Amnis Image Straining, Flow Cytometry

Starting Material

Human primary T cells

Protocol Overview

DRAQ5 was added to already immunostained cells and incubated at RT for 10 minutes prior to analysis.

Tips

Make sure to cover your tubes or have them in the dark while adding DRAQ5.

Results Summary

Nuclei staining worked great and it was used to determine nuclear co-localization of class I hormone receptors/

DOI or PMID #

10.1089/gg.2017.0014

Additional Notes

N/A

Image Gallery

Summary

The Good

Fast staining and very bright.

The Bad

Small volume in vial, might need several if big experiments are being performed.

The Bottom Line

Great and fast nuclear staining similar to DAPI staining but read in laser 642 instead of 488.

Join the discussion