Rhod-2 AM, fluorescent Ca2+ indicator from Abcam

Product Specs
ItemRhod-2 AM, fluorescent Ca2+ indicator
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab142780
Quantity1 mg
Purity0.93
TypeChemical
Molecule NameRhod-2 AM

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(1) Harpagide attenuates cerebral ischemic injury by modulating mitochondrial calcium homeostasis associated with the AMPK–MCU axisScientific ReportsJune 1, 2026Ke Wang, Huai-yu Liu, Yue Wang, Gen-zheng Su, Jin-lai Gao, Yan-Ping Wang, Zhi Heassay kit (S0027, Shanghai Beyotime Biotechnology, Shanghai, China), a Rhod-2 AM fluorescent probe (ab142780, Abcam), a 2’,7'-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe (S0033S, Shanghai Beyotime

(2) Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS productionJournal of VirologyMay 19, 2026Min Huang, Chengyin Liukang, Huiming Yang, Lu Sun, Lihua Tang, Pinyi Lv, Feixuan Hou, Ye Zhao, Guozhong Zhang, Jing Zhaomeasurement Following IBV infection or drug treatment, CEK cells were incubated with 5 μM Rhod-2 AM (Abcam, ab142780) at 37°C for 30 min in the dark. After incubation, the cells were washed twice with PBS and resuspended

(3) A Viscous DES‐AAV‐ Foxo1 Delivery System With High Transfection Efficiency for the Treatment of Corneal Endothelial Dysfunction by Restoring Mitochondria‐ER ContactsAdvanced ScienceMay 3, 2026Hongran Zhao, Xiaoyu Li, Hongwei Wang, Zongyi Li, Qun Wang, Yangyang Zhang, Xu Jing, Xia Qi, Qingjun Zhou, Shengqian Dou, et al.and MitoSOX Staining CEnCs were cultured in serum free medium containing 5 µM Rhod‐2 AM (Abcam, Cat# ab142780) and 200 nM Mito‐Tracker Green (Beyotime, Cat# C1048) for 35 min at 37°C. CEnCs were cultured in HBSS

(4) ChREBP-β Exacerbates Renal Tubular Disorders Caused by Fructose via ATF4FASEB JApril 30, 2026Ting Fang, Xinyu Yang, Xiaoqing Deng, Jingyi Wang, Ting Li, Hang Guo, Bei Sun, Liming ChenMeasurements Human kidney‐2 (HK2) cells were treated with 2 μM Fura‐2 AM (ab120873, Abcam) and 1 μM Rhod2‐AM (ab142780, Abcam) in a Pluronic‐F27 solution for 15 min at 37°C, followed by a wash. Time‐lapse fluorescence imaging

(5) JP2 and JCN Crosstalk Abrogate MURF1-Mediated JCN Ubiquitination and Degradation in CardiomyocytesJACC Basic Transl SciApril 17, 2026Xiaoyun Ji, Yifan Huang, Jinxi Wang, Zhifeng Dong, Rui Ni, Zijun Su, Dong Zheng, Guo-Chang Fan, Douglas L. Jones, Long-Sheng Song, et al.Methods.45 Briefly, after treatments, cardiomyocytes were stained with Rhod-2 AM (2 μmol/L, Abcam, ab142780) for 30 minutes at room temperature, washed twice and transferred to a bath chamber. The cardiomyocytes

(6) ChREBP‐β Exacerbates Renal Tubular Disorders Caused by Fructose via ATF4FASEB JApril 13, 2026Ting Fang, Xinyu Yang, Xiaoqing Deng, Jingyi Wang, Ting Li, Hang Guo, Bei Sun, Liming ChenMeasurements Human kidney‐2 (HK2) cells were treated with 2 μM Fura‐2 AM (ab120873, Abcam) and 1 μM Rhod2‐AM (ab142780, Abcam) in a Pluronic‐F27 solution for 15 min at 37°C, followed by a wash. Time‐lapse fluorescence imaging

(7) Clinically Relevant Doses of Remimazolam Modulate Cardiac Electrophysiology: Late Repolarization Prolongation and Increased Conduction Dispersion With Preserved QTcCardiol Res PractApril 13, 2026Zijun Wang, Ying Cao, Gao Su, Yanyan Feng, Rongfeng Yang, Xue Bai, Hong Gaoloading. For dual‐parameter optical mapping, RH237 (Chem Cruz, sc‐499456; 1 μg/mL) and Rhod‐2 AM (Abcam, ab142780; 1 μg/mL) were introduced sequentially. Illumination was provided by 530 nm light‐emitting diode (LED)

(8) Pim1 Serves as a Therapeutic Target for Inflammatory Arthritis via Mitochondrial Metabolism and Th17 Cell DifferentiationResearch (Wash D C)February 27, 2026Zepeng Su, Yipeng Zeng, Qibo Li, Jinteng Li, Guan Zheng, Weihao Zhang, Zipeng Xiao, Zibin Chen, Yangfeng Lin, Ziqian Liu, et al.#C2001S) for detection of the mitochondrial membrane potential, stained with 5 μM Rhod-2AM (Abcam, #ab142780) for detection of mito-Ca2+, and stained with 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA;

(9) MiR-499-5P/PACS2/TRPV1 Axis Maintains Mitochondrial Homeostasis and Left Ventricular Function after Extreme Cold StressJ Cardiovasc Transl ResFebruary 5, 2026Renzheng Chen, Yan Ma, Sijia Chen, Cheng Qin, Hui Li, Naiyuan Sun, Feng Caoseeded on glass-bottomed cell culture dishes and incubated with 1μM of the calcium indicator Rhod2-AM (ab142780, Abcam) at 37 °C in the dark for 30 min. Next, cells were washed twice with Hanks’ Balanced Salt Solution

(10) Super mitochondria-enriched extracellular vesicles enable enhanced mitochondria transferNat CommunOctober 27, 2025Yi Wang, Hao-Yuan Yu, Zi-Juan Yi, Lian-Yu Qi, Jing-Song Yang, Hai-Xin Xie, Min Zhao, Na-Hui Liu, Jia-Qi Chen, Tian-Jiao Zhou, et al.HuaBio (Hangzhou, China). Anti-Vimentin mAb (ab92547), Anti-Complex I mAb (ab109798) and Rhod-2 AM (ab142780) were purchased from Abcam (Cambridge, UK). The siRNA targeting IP3R (siIP3R) from Riobio Company Limited

(11) Abnormal Ca2+ handling and reduced Ito contribute to citalopram-induced QT prolongation and cardiac arrhythmiasFrontiers in PharmacologySeptember 4, 2025Yangpeng Li, Jiamin Xie, Yuqing Zheng, Jianhong Li, Min Zhao, Li Liu, Hongping Shen, Ming Lei, Xiaoqiu Tan, Juan Xiao, et al.Then, 15 µL stock solution of Rhod2-AM (final concentration 0.356 mM, 1 mg/mL, stock solution, Cat: ab142780, Abcam, Cambridge, United Kingdom) and 15 µL Pluronic F127 (20% w/v in DMSO, Cat: P3000MP, Invitrogen,

(12) Targeting CB1R Rewires Ca2+-Dependent Mitophagy to Promote Nerve RegenerationTheranosticsAugust 11, 2025Ningning Wang, Weizhen Li, Tuo Yang, Baolong Li, Chuikai Meng, Xiongyao Zhou, Jialu Sun, Kaiming Yu, Shusen Cui, Rangjuan Caoactivity. Calcium imaging using Rhod-2 AM Mitochondrial Ca2+ levels were measured using Rhod-2 AM (Abcam, ab142780). Cells were incubated with 5 μM Rhod-2 AM in glass-bottom culture dishes at 37 °C for 30 min in the

(13) Mathematical model of the zebrafish ventricular cardiomyocyte action potential and calcium transientJ PhysiolJuly 31, 2025Ludovica Cestariolo, Zachary D. Long, Arie O. Verkerk, Jose M. Ferrero, T. Alexander Quinn, Jose F. Rodriguez MatasTwo different Ca2+‐sensitive dyes with different affinities for Ca2+ were used: (1) 10 µM Rhod‐2 AM [ab142780, Abcam, Cambridge, UK; dissociation constant (K d) ≈ 0.57 µM) or (2) 5 µM Fluo‐5F AM (20560, AAT Bioquest,

(14) Filamented Light (FLight) Bioprinting of Mini‐Muscles with Self‐Renewal PotentialAdvanced MaterialsJuly 23, 2025Hao Liu, Michael Winkelbauer, Jakub Janiak, Patrick Weber, Ali Kerem Kalkan, Inseon Kim, Parth Chansoria, Ori Bar‐Nur, Marcy Zenobi‐Wongfluorescent Ca2+ indicator). Briefly, 10 µL of Rhod‐2 AM calcium dye (Stock solution: 1 mg mL−1, Abcam, AB142780) in dimethyl sulfoxide (DMSO) was carefully mixed with 10 µL of Pluronic F‐127 (diluted in DMSO as 20%

(15) Oleanolic acid enhanced the anticancer effect of fluorouracil by regulating Ca2+ levels in hepatocellular carcinoma cellsBraz J Med Biol ResApril 14, 2025Jing Dong, Yin Gao, Penghui Li, Ping Chen, Yanxin Lv, Yanan Liu, Song Zhang, Minglong Zhang, Yu Wangindicator (Thermo Fisher Scientific, USA, M14206); Rhod-2AM calcium ion fluorescent probe (Abcam, UK, ab142780); D-Hanks’ solution (PYG0079, Wuhan Doctoral Biotechnology Co., Ltd., China); MTT Cell Proliferation

(16) Oleanolic acid enhanced the anticancer effect of fluorouracil by regulating Ca2+ levels in hepatocellular carcinoma cellsBraz J Med Biol ResApril 14, 2025Jing Dong, Yin Gao, Penghui Li, Ping Chen, Yanxin Lv, Yanan Liu, Song Zhang, Minglong Zhang, Yu Wangindicator (Thermo Fisher Scientific, USA, M14206); Rhod-2AM calcium ion fluorescent probe (Abcam, UK, ab142780); D-Hanks’ solution (PYG0079, Wuhan Doctoral Biotechnology Co., Ltd., China); MTT Cell Proliferation

(17) IL-1β enhances susceptibility to atrial fibrillation in mice by acting through resident macrophages and promoting caspase-1 expressionNature Cardiovascular ResearchFebruary 6, 2025Oscar Moreno-Loaiza, Vinicius Cardoso Soares, Manuela de Assumpção Souza, Narendra Vera-Nuñez, Ainhoa Rodriguez Rodriguez de Yurre Guirao, Tatiana Pereira da Silva, Ana Beatriz Pozes, Larissa Perticarrari, Evelin Monteiro, Maria Clara Albino, et al.saturated with O2. After approximately 5 min of stabilization, a fluorophore dye (Rhod-2- AM – ABCAM – #ab142780) diluted in oxygenated Tyrode’s solution was perfused. After dye-loading, Blebbistatin (4 μM) (Selleckchem,

(18) Dual calcium-voltage optical mapping of regional voltage and calcium signals in intact murine RyR2-R2474S heartsJournal of Molecular and Cellular Cardiology PlusDecember 1, 2024Yangpeng Li, Zhu Liu, Christopher O'Shea, Jianhong Li, Xian Luo, Tangting Chen, Xianhong Ou, Weichao Liu, Guoliang Hao, Christopher L.-H. Huang, et al.stock solution Cat: S1109, Thermo Fisher Scientific, USA) and Rhod2-AM (1 mg/mL, stock solution, Cat: ab142780, Abcam, UK) were perfused to enable simultaneous membrane voltage (Vm) and intracellular Ca2+ measurements

(19) Brain-to-heart cholinergic synapse-calcium signaling mediates ischemic stroke-induced atrial fibrillationTheranosticsOctober 7, 2024Yingran Liang, Gongxin Wang, Siwen Fan, Junyi Zhang, Shuang He, Guixiang Pan, Guoliang Hao, Yan ZhuLemeitian Pharmaceutical Technology Co., Ltd (Chengdu, China). Blebbistatin (ab120425) and Rhod-2AM (ab142780) were obtained from Abcam Plc (Cambridge, United Kingdom). F127 (P3000MP) was purchased from Invitrogen

(20) Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventionsNature communicationsSeptember 12, 2024Yang P, Zhu L, Wang S, Gong J, Selvaraj JN, Ye L, Chen H, Zhang Y, Wang G, Song W, et al.eliminate motion artifacts. Intracellular calcium changes were measured using 1 mg/mL RHOD-2AM (Abcam, ab142780). The entire heart was illuminated with 549 nm excitation light (MappingLab, LEDC-2001), and CMOS cameras

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Rhod-2 AM, fluorescent Ca2+ indicator from Abcam

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Rhod-2 AM, fluorescent Ca2+ indicator from Abcam

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