Isothermal Amplification Buffer Pack from New England Biolabs

Product Specs
ItemIsothermal Amplification Buffer Pack
CompanyNew England Biolabs
Price $32.00 Inquire
Catalog NumberB0537S
Quantity6 ml
ApplicationsDNA Analysis, Amplification
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.this point. Next 40 µl per plug of reaction mix containing 5 µl isothermal amplification buffer (NEB, B0537S), 3 µl 100 mM MgSO4 (NEB, B1003S), 2 µl 10 mM dNTPs, and 1 µl Bst 2 WarmStart DNA polymerase (NEB, M0538S)

(2) A portable and low-cost fluorescence reader for near-patient nucleic acid amplification assaysBiomed MicrodevicesFebruary 14, 2026Ethan Rosenfeld, Kathryn Pacheco, Evan Benke, Ian M. White, Don L. DeVoeused as a model sample in these experiments. Each sample included 1X isothermal amplification buffer (B0537S, New England Biolabs), trehalose (15% by weight), 0.6 mM dNTPs (N0447S, New England Biolabs), 1X EvaGreen

(3) Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tagBiotechnology & Biotechnological EquipmentDecember 31, 2025Enos C. Kline, Rose Duong, Qin Wang, Barry R. LutzMolecular Biology Grade Water (Corning®, 46–000-CM), 2 μL 10x Isothermal Amplification Buffer (NEB, B0537S), 0.5 μL 10 mM dNTP Solution Mix (NEB, N0447S), 1 μL of 10 μM RT primer (Supplemental Table ST1, IDT),

(4) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruziMicrobiology SpectrumNovember 4, 2025Sneider Alexander Gutierrez Guarnizo, Beth Jessy Condori, Luciana Basma, Shirley Equilia, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.(Sigma-Aldrich, CN: B0300-5VL), 1× Isothermal Amplification Buffer (20 mM Tris-HCl pH 8.8, NEB, CN: B0537S), and 8 U Bst Polymerase 2.0 WarmStart (NEB, CN: M0537L). Two microliters of DNA template were added

(5) Kinetic analysis and engineering of thermostable Cas12a for nucleic acid detectionNucleic Acids ResJune 13, 2025Juan Pan, Megumu Mabuchi, Daniel W Kneller, Ryan T Fuchs, Jennifer L Curcuru, Esta Tamanaha, Nathan A Tanner, G Brett Robb, Ivan R Corrêa(#B6002S), Proteinase K (#P8107S), Bst 2.0® DNA polymerase (#M0537S), Isothermal Amplification Buffer (#B0537S), WarmStart® RTx Reverse Transcriptase (#M0380L), dNTP solution mix (#N0447L), Lambda DNA (#N3011S),

(6) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruzimedRxivJanuary 13, 2025Sneider Alexander Gutierrez Guarnizo, Luciana Basma, Shirley Equilia, Beth Jessy Condori, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.(Sigma-Aldrich, CN: B0300–5VL), 1X Isothermal Amplification Buffer (20 mM Tris-HCl pH 8.8, NEB, CN: B0537S), and 8 U Bst Polymerase 2.0 WarmStart (NEB, CN: M0537L). Two microliters of DNA template were added

(7) Population expansion from central plain to northern coastal China inferred from ancient human genomesiScienceDecember 20, 2024Baitong Wang, Daohua Hao, Yu Xu, Kongyang Zhu, Rui Wang, Xiaomin Yang, Qu Shen, Mengting Xu, Tianyou Bai, Hao Ma, et al.acid,Sigma,695092 Sodium acetate,Sigma,S7899 Tween 20,Sigma,P7947 Isothermal amplification buffer,NEB,B0537S Deoxynucleotide (dNTP) solution mix,NEB,N0447L Bst 2.0 DNA polymerase,NEB,M0537L AMPure XP Beads,Beckman,A63881 Agarose,Biowest,111860 Tris-EDTA buffer solution

(8) Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complexCellNovember 27, 2024Owen T. Tuck, Benjamin A. Adler, Emily G. Armbruster, Arushi Lahiri, Jason J. Hu, Julia Zhou, Joe Pogliano, Jennifer A. DoudnaSuperose 6 increase 10/300 GL column,Cytiva,Cat#29091598 Isothermal Amplification Buffer,New England Biolabs,Cat#B0537S E. coli single-stranded DNA binding protein (SSB),QIAGEN,Cat#Y9030L Phage T4 single-stranded DNA binding

(9) Comparison and Optimization of Simple DNA Extraction Methods for LAMP-Based Point-of-Care Applications Employing Submillimeter Skin BiopsiesACS OmegaSeptember 17, 2024Juan M. Boza, Jason Cade Manning, David C. EricksonReal-Time LAMP (Figure 2a–d). The LAMP assay composition (Table S3) includes 10× isothermal buffer (NEB B0537S), 100 mM MgSO4 (NEB B1003S), 10 mM dNTPs (NEB N0447L), and BST 2.0 WarmStart polymerase (NEB M0538S),

(10) Protocol for a comprehensive pipeline to study ancient human genomesSTAR ProtocolsJune 21, 2024Kongyang Zhu, Haifeng He, Le Tao, Hao Ma, Xiaomin Yang, Rui Wang, Jianxin Guo, Chuan-Chao Wangacid,Sigma,695092 Sodium acetate,Sigma,S7899 Tween 20,Sigma,P7947 Isothermal amplification buffer,NEB,B0537S Deoxynucleotide (dNTP) solution mix,NEB,N0447L Bst 2.0 DNA polymerase,NEB,M0537L AMPure XP Beads,Beckman,A63881 Agarose,Biowest,111860 Tris-EDTA buffer solution

(11) Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)Sci RepMarch 11, 2024May Khat Nwe, Nisachon Jangpromma, Lapatrada TaemaitreeTris-HCl, 100 mM (NH4)2SO4, 500 mM KCl, 20 mM MgSO4, 1% Tween® 20, pH 8.8 @ 25 °C], 10 ×, 1.25 µL, NEB, B0537S), EvaGreen dye (20 ×, 0.625 µL, Biotium). LAMP products were monitored using a real-time PCR machine,

(12) Relationships of Late Pleistocene giant deer as revealed by Sinomegaceros mitogenomes from East AsiaiScienceDecember 15, 2023Bo Xiao, Alba Rey-lglesia, Junxia Yuan, Jiaming Hu, Shiwen Song, Yamei Hou, Xi Chen, Mietje Germonpré, Lei Bao, Siren Wang, et al.,New England Biolabs,Cat#C2312 Quick Ligase buffer,New England Biolabs,Cat#B2200S Isothermal buffer,New England Biolabs,Cat#B0537S Q5 High-Fidelity DNA Polymerase,New England Biolabs,Cat#M0491V D1000 screen tape,Agilent,Cat#5067-5582 dsDNA

(13) Simultaneous and rapid colorimetric detection of distinct miRNAs using Split-LAMPFrontiers in Bioengineering and BiotechnologyNovember 2, 2023Yi Jing Chua, Steven Poh Chuen Sim, Medha Shridharan, Yiqi Seowhydroxide (catalog number BUF-1530, First Base), and was stored at −20°C. Isothermal buffer (catalog number B0537S, New England Biolabs) comprises of 200 mM Tris-HCl pH 8.8, 100 mM Ammonium Sulfate, 500 mM Potassium

(14) Development of a loop-mediated isothermal amplification (LAMP)-based electrochemical test for rapid detection of SARS-CoV-2iScienceSeptember 15, 2023Khushboo Borah Slater, Muhammad Ahmad, Aurore Poirier, Ash Stott, Bianca Sica Siedler, Matthew Brownsword, Jai Mehat, Joanna Urbaniec, Nicolas Locker, Yunlong Zhao, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, Isothermal amplification buffer,New England Biolabs,B0537S 6mM MgSO4,New England Biolabs,B1003 1.4mM dioxyribonucleotides dNTPs,New England Biolabs,N0447S 320U/mL

(15) Click display: a rapid and efficient in vitro protein display method for directed evolutionNucleic acids researchSeptember 8, 2023Zeng Y, Woolley M, Chockalingam K, Thomas B, Arora S, Hook M, Chen Z.all the variable regions, were incubated in 1× Isothermal Amplification Buffer (New England Biolabs, B0537S) in the presence of MgSO4 (final 6 mM) and dNTP (final 1.4 mM each) at 94°C for 2 min followed by slow

(16) Droplet-based high-throughput single microbe RNA sequencing by smRandom-seqNat CommunAugust 23, 2023Ziye Xu, Yuting Wang, Kuanwei Sheng, Raoul Rosenthal, Nan Liu, Xiaoting Hua, Tianyu Zhang, Jiaye Chen, Mengdi Song, Yuexiao Lv, et al.New England Biolabs, Cat. M0202S), dNTP (10 mM each), 1× isothermal amplification buffer (New England Biolabs, Cat. B0537S), nuclease-free water was prepared, then split into a round-bottom 96-well plate. The hydrogel beads

(17) Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMPLab on a ChipJuly 25, 2023Daniel W. Weisgerber, Krzysztof Langer, Venice Servellita, Peng Xu, Charles Y. Chiu, Adam R. Abateto RT-ddPCR and RT-qPCR. A LAMP master mix is prepared with 1× isothermal amplification buffer (NEB B0537S), 7.5 mM MgSO4 (NEB B1003S), 1.875 mM dNTPs (ThermoFisher Scientific R1122), 8 U Bst 2.0 WarmStart DNA

(18) Reduced Expression of TMEM16A Impairs Nitric Oxide-Dependent Cl− Transport in Retinal Amacrine CellsFrontiers in Cellular NeuroscienceJuly 27, 2022Tyler Christopher Rodriguez, Li Zhong, Hailey Simpson, Evanna Gleason(Thermo Fisher, EN0771). DNA was digested at 37°C for 2min. Reverse transcription components (NEB, B0537S, M0314S, and N0446S) were added to a final volume of 15μl, and RNA was denatured at 75°C for 5min. The

(19) Historical Genomes Reveal the Genomic Consequences of Recent Population Decline in Eastern GorillasSSRN Electronic JournalApril 2, 2022Tom van der Valk, David Díez-del-Molino, Tomas Marques‐Bonet, Katerina Guschanski, Love DalénT4 DNA ligase Thermofisher Scientific Cat#: EL0014 Isothermal Amplification buffer New England Biolabs Cat#: B0537S Bst polymerase 2.0, Large Fragment New England Biolabs Cat#: M0275S Pfu Turbo Cx Hotstart DNA Buffer Agilent technologies

(20) Preparation and Use of Cellular Reagents: A Low‐resource Molecular Biology Reagent PlatformCurrent ProtocolsApril 1, 2022Sanchita Bhadra, Inyup Paik, Jose‐Angel Torres, Stéphane Fadanka, Chiara Gandini, Harry Akligoh, Jenny Molloy, Andrew D. Ellingtonfor up to 12 months. 10X Isothermal buffer (may also be purchased from New England Biolabs, cat. No. B0537S) 200 mM Tris-HCl, pH 8.8100 mM (NH4)2SO4500 mM KCl20 mM MgSO41% Tween® 20pH 8.8 at 25°CStore at −20

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