RNA Loading Dye (2x) from New England Biolabs

Product Specs
ItemRNA Loading Dye (2x)
CompanyNew England Biolabs
Price $56.00 Inquire
Catalog NumberB0363S
Quantity4 ml
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Uridine analogs prevent stress granule formation, not by blocking PKR recognition, but by inhibiting the synthesis of T7 RNA Polymerase byproductsRNAMay 1, 2026Sean J. Ihn, Emily Jiang, Nevraj S. KeJiou, Yifan E. Wang, Laura Farlam-Williams, Alexander F. Palazzo, Hyun O. LeeColumns (Cytiva 27532501). Purified in vitro transcribed RNA was resuspended in RNA loading dye (NEB B0363S) and heated at 70°C for 10 min followed by rapid cooling on ice. Four micrograms of total RNA was loaded

(2) Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducensNucleic Acids ResApril 24, 2026Benjamin F Jepson, Eric J Wolf, Anthony R Dawson, Jingmin Jin, Vladimir Potapov, Nan Dai, Ivan R Corrêa Jr., Erbay Yigitincubated at room temperature for 15 min to inactivate the enzyme. Next, 10 μl of 2× RNA loading dye (NEB, B0363S) was added, and samples were heated at 70°C for 5 min. A total of 18 μl from each reaction was loaded

(3) A Simple and Easy Method for RNA Extraction from the Cyanobacterium Synechocystis sp. PCC 6803BIO-PROTOCOLApril 5, 2026Bharat Kumar Majhi, Julian J. Eaton-Rye1 kb Plus DNA ladder (Thermo Fisher Scientific, catalog number: 10787018) 19. RNA loading dye (NEB, catalog number: B0363S) 20. DNA loading dye (NEB, catalog number: B7024S) 21. BG-11 media (Gibco, catalog number: A1379901) 22.

(4) Functional siRNA Delivery via Jet Nebulization: Proof-of-Concept IL-1ß Silencing in Macrophage-like THP-1 CellsInt J Mol SciMarch 23, 2026Duy Bao Tran Nguyen, Ahmed S. M. Ali, Dongwei Wu, Johanna Berg, Daniel C. Lauster, Jens Kurreck, Beatrice Tolksdorfa single band if both strands are the same length. All samples were mixed with 2× RNA Loading Dye (B0363S, New England Biolabs, Ipswich, MA, USA). A 1 μg aliquot of each siRNA was loaded onto the polyacrylamide–urea

(5) Cell-free DNA methylome and fragmentome analysis for relapse monitoring of Ewing sarcomaEMBO Molecular MedicineMarch 6, 2026Sophie A Richardson, Aram Safrastyan, Mina Karimpour, Gayatri Gulati, Patrick J B Harker, Alan Redfern, Simon P Pearce, Vsevolod J Makeev, Bernadette Brennan, Alexander T J Lee, et al.Solution,Fisher,BP1332-1 Gel Star Nucleic Acid Gel Stain,Lonza,50535 RNA Loading Dye (2X),New England Biolabs,B0363S ssRNA Ladder (50 bp),New England Biolabs,N0364S Gel Loading Dye (6X),New England Biolabs,B7021S DNA

(6) DNMT2 inhibits anaplastic thyroid cancer progression by downregulating 5’tiRNAGly-GCC productionCell Death & DiseaseFebruary 21, 2026Ruixin Zhou, Baizhao Li, Mingyu Cao, Zhijing Wu, Fada Xia, Xinying Liand incubated at 37 °C for 1 min. Immediately afterwards, an equal volume of 2×RNA Loading Dye (Cat#B0363S, NEW ENGLAND Biolabs) was added, and the tRNA digestion products were analyzed by 15% urea-denaturing

(7) Conserved shifts in sperm small non-coding RNA profiles during mouse and human agingThe EMBO JournalJanuary 20, 2026Junchao Shi, Xudong Zhang, Chen Cai, Shichao Liu, Jiancheng Yu, Emma R James, Lihua Liu, Benjamin R Emery, Megan R McMurray Bires, Elizabeth Torres-Arce, et al.total RNAs The RNA sample, mixed with an equal volume of 2× RNA loading dye (New England Biolabs; B0363S), was incubated at 75 °C for 5 min. The mixture was loaded into 15% (wt/vol) urea polyacrylamide gel

(8) Genome-wide profiling of RNA 2’-O-methylation in neurons and identification of orphan snoRNA targetsResearch SquareJanuary 20, 2026Gordon Carmichael, Xuan Ye, Yaling Liu, Yinzhou Zhu, Saran Alshawi, Brittany Elliott, Christopher Holley, Jean-Denis Beaudoin, Brenton R. Graveleyroom temperature for 60 minutes. Prior to electrophoresis, 2× RNA loading dye (New England Biolabs, #B0363S) was added to the RNA sample, which was then incubated at 72°C for 2 minutes and placed on ice for 3

(9) A programmable ribozyme for RNA signal transductionNature CommunicationsJanuary 10, 2026Mandy Yu Theng Lim, Chermaine Tan, Charannya Sozheesvari Subhramanyam, Shi Jie Teo, Louis DeFalco, Samuel Kevin Pasaribu, Chong Hui Koh, Dheeraj Rayamajhi, Jieying Chi, Shengnan Li, et al.visualisation of cleavage product Cleavage reaction product was directly mixed with 2× RNA Loading Dye (#B0363S, New England Biolabs, USA), then heat-denatured at 70 °C for 10 min and separated on a 10% denaturing

(10) Highly efficient expression of DNA-peptide conjugates in growth-arrested cellsNature CommunicationsJanuary 7, 2026Zulfiqar Y. Mohamedshah, Chih-Chin Chi, Ember M. Tota, Alexis C. Komor, Neal K. Devarajammonium persulfate. Samples (150–200 ng DNA oligonucleotide) were diluted in 2× RNA loading dye (NEB B0363S), denatured at 98 °C for 5 min, and loaded into the gel. Electrophoresis was performed at 210 V for

(11) Structural basis for non-AUG translation regulation by 5MPsbioRxivDecember 31, 2025Ximena Zottig, Chun-Ying Huang, Zahra Seraj, Nikolaus Grigorieff, Andrei A. Korostelevfor a final volume of 20 μl. The reactions were stopped by the addition of 2× RNA loading dye (NEB; B0363S), and the samples were heated at 70 °C for 10 min. mRNA integrity was assessed by 1% denaturing formaldehyde

(12) A cholesterol-responsive hepatic tRNA-derived small RNA regulates cholesterol homeostasis and atherosclerosis developmentNat CommunDecember 15, 2025Xiuchun Li, Rebecca Hernandez, Xudong Zhang, Sijie Tang, Xiaohong Yuan, Jing Wu, Kathy Pham, Hukam C. Rawal, Erica C. Heinrich, Shenglong Zhang, et al.The hepatic RNA samples was mixed with an equal volume of 2× RNA loading dye (New England Biolabs, B0363S) and incubated at 75 °C for 5 min. The mixture was loaded into 15% (wt/vol) urea polyacrylamide gel

(13) Efficient circRNA Detection Using the Processive Reverse Transcriptase uMRTBIO-PROTOCOLOctober 20, 2025Ruben Warkentin, Anna Marie Pylenumber: 10777019) 5. CircRNA template or cell extracted RNA 6. RNA loading dye (2×) (NEB, catalog number: B0363S) 7. Reverse transcription primer (should not span circRNA junction) 8. DreamTaq PCR Master Mix (Thermo Fisher Scientific

(14) Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferationPLOS BiologySeptember 16, 2025Ana Luisa Dian, Lucilla Fabbri, Antoine Moya-Plana, Giuseppina Claps, Juliana C. Ferreira, Céline M. Labbé, Virginie Quidville, Sylvain Martineau, Dorothée Baille, Laetitia Besse, et al.resuspended in RNAse-free water and incubated for 5 min in 1× RNA loading dye (New England Biolabs, #B0363S) at 80°C at 1,000 rpm. Samples were loaded onto a denaturing 6% TBE-urea gel and migrated at 70 V for

(15) Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate statesNat CommunAugust 26, 2025Bruna Favetta, Huan Wang, Jasmine Cubuk, Arjun Singh, Mayur Barai, Cesar Ramirez, Haiyan Zheng, Adam J. Gormley, N. Sanjeeva Murthy, Gregory Dignon, et al.T2040L). Purified RNA was verified for purity and size using an agarose gel and RNA Gel Loading Dye (NEB B0363S) and RiboRuler High Range RNA Ladder (Thermo Scientific SM183). Concentration was measured using a Nanodrop

(16) Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boostingCell Reports MedicineAugust 19, 2025Yanyan Wang, Liangru Lin, Xinyue Wang, Jing Li, Qian Pan, Haomeng Kou, Jie Yin, Fei Gao, Xinyuan Liao, Chenchen Zhang, et al.mixture was incubated at 37°C for either 5 or 15 min. To terminate the reaction, RNA loading dye (NEB, #B0363S) was added, and the RNA was subsequently separated using agarose gel electrophoresis. CircRNA transfection

(17) PANDORA-seq reveals human sperm sncRNA signature endowed with sperm quality assessmentGenes & DiseasesAugust 18, 2025Ruofan Huang, Yiting Yang, Wenlin Jiang, Zheng Cao, Junchao Shi, Xiao-Ou Zhang, Yunfang Zhangdescribed.26 Briefly, total RNA was denatured at 95 °C for 5 min with RNA loading dye (New England Biolabs, B0363S), separated by 15% denatured urea-PAGE, and stained with SYBR Gold (Thermo Fisher, S11494). 15–50 nucleotides

(18) Lifetime of ground conformational state determines the activity of structured RNANature Chemical BiologyJuly 1, 2025Rhese D. Thompson, Derek L. Carbaugh, Joshua R. Nielsen, Ciara M. Witt, Edgar M. Faison, Rita M. Meganck, Atul Rangadurai, Bo Zhao, Jeffrey P. Bonin, Nathan I. Nicely, et al.northern blotting. Northern blot First, 1.5 μg of RNA was resuspended in 2× RNA loading dye (New England Biolabs, B0363S), incubated at 65 °C for 7 min and cooled on ice. Samples were separated on a 10% denaturing polyacrylamide

(19) Chemical and topological design of multicapped mRNA and capped circular RNA to augment translationNature BiotechnologyJuly 1, 2025Hongyu Chen, Dangliang Liu, Abhishek Aditham, Jianting Guo, Jiahao Huang, Franklin Kostas, Kamal Maher, Mirco J. Friedrich, Ramnik J. Xavier, Feng Zhang, et al.points, 5 μl of reaction mixture was aliquoted out into 10 μl of denaturing gel-loading buffer (NEB, B0363S, with 5 M urea) and frozen at −80 °C. The fractions were determined using Novex 15% TBE–urea gel (Invitrogen,

(20) Conserved long-range interactions are required for stable folding of orthoflaviviral genomic RNANucleic Acids ResJune 16, 2025Michael Z Palo, Betty Ha, Christopher P Lapointe, Carlos Alvarado, John Janetzko, Jan E Carette, Joseph D Puglisi, Elisabetta Viani Puglisimg/ml creatine phosphokinase. Reactions were mixed with an equal volume of RNA Loading Dye, 2× (NEB, B0363S) and heated at 95°C for 2 min before loading onto 10% polyacrylamide-TBE gels containing 7.5 M urea.

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