Calcein AM, fluorescent dye for cell viability from Abcam

Product Specs
ItemCalcein AM, fluorescent dye for cell viability
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab141420
Quantity50 µg
Purity0.98
TypeChemical
Molecule NameCalcein AM

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Cambridge Biomedical Campus

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United Kingdom

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+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Modelling cigarette smoke-induced lung vascular dysfunction using an alveolus-on-chipMater Today BioJuly 6, 2026Abilash Ravi, Tarek Gensheimer, Annemarie van Schadewijk, Xinhui Wu, Jill R. Johnson, Martin C. Harmsen, Reinoud Gosens, Pieter S. Hiemstra, Andries D. van der Meer, Anne M. van der Doesin a total volume of 40 mL. To visualize viable THP-1 monocytes, cells were stained with Calcein-AM (ab141420, Abcam, USA). The Calcein-AM stock was diluted to prepare a 0.2 μM working solution in HBSS (Thermo Fisher Scientific

(2) Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon NanowiresACS NanoOctober 28, 2025Tania Assaf, Layan Habib, Adi Sasson, Shiri Karni-Ashkenazi, Menahem Y. RotenbergPBS. Cell Staining For 3D z-stack acquisition and intracellular visualization of SiNWs, Calcein AM (ab141420, Abcam) was used as a viability stain. After a 30 min incubation, cells were washed three times with

(3) In Vitro Investigation of 3D Printed Hydrogel Scaffolds with Electrospun Tidemark Component for Modeling Osteochondral InterfaceGelsNovember 15, 2024Victoria Effiong Effanga, Dana Akilbekova, Fariza Mukasheva, Xiao Zhao, Dilhan M. Kalyon, Cevat EriskenSLCG4328), and sodium borohydride (#S0480 Tokyo Chemical Industry, Tokyo, Japan, YDTOG-PY). Calcein AM (ab141420, APN20034-1-2), ethidium homodimer (ab145323, GR3192625-47), and Hoechst 33342 (ab228551, GR3436460-2)

(4) Magnesium lithospermate B enhances the potential of human-induced pluripotent stem cell-derived cardiomyocytes for myocardial repairChinese Medical JournalAugust 5, 2024Chengming Fan, Kele Qin, Chukwuemeka Daniel Iroegbu, Kun Xiang, Yibo Gong, Qing Guan, Wenxiang Wang, Jun Peng, Jianjun Guo, Xun Wu, et al.(ab150074); Alexa Fluor 488-conjugated donkey anti-mouse IgG (H&L) (ab150105); and calcein Acetoxymethyl (ab141420) were procured from Abcam (Cambridge, UK). Antibodies against F4/80 (#70076), cleaved-caspase-3 (9664s),

(5) Impact of Hydroxyapatite on Gelatin/Oxidized Alginate 3D-Printed Cryogel ScaffoldsGelsJune 18, 2024Ainur Zhanbassynova, Fariza Mukasheva, Madi Abilev, Dmitriy Berillo, Alexander Trifonov, Dana AkilbekovaMaterials ALP kit (ab83369), Hoechst 33,342 (ab228551), ethidium homodimer (ab145323), and Calcein AM (ab141420) were obtained from Abcam (Cambridge, UK). β-glycerophosphate disodium salt hydrate, 99% (G9422), sodium

(6) Hydrogel-Based Oxygen and Drug Delivery Dressing for Improved Wound HealingACS OmegaJune 4, 2024Wen Ren, Mia Sands, Xiaoxue Han, Michael Tsipursky, Joseph Irudayaraj(Dublin, OH). Propidium iodide was ordered from ThermoFisher Scientific (Waltham, MA). Calcein AM (ab141420) was purchased from Abcam (Boston, MA). The chemicals were used as received without further purification.

(7) Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healingNature communicationsApril 23, 2024Han X, Saengow C, Ju L, Ren W, Ewoldt RH, Irudayaraj J.anti-Rabbit IgG (H + L) Secondary Antibody (1:50). ROS/Superoxide detection assay kit (ab139476), calcein AM (ab141420), DAPI (ab228549) were purchased from Abcam. Human mesenchymal stem cell marker antibody panel was purchased

(8) Magnesium lithospermate B enhances the potential of human-induced pluripotent stem cell-derived cardiomyocytes for myocardial repairChin Med J (Engl)January 15, 2024Chengming Fan, Kele Qin, Chukwuemeka Daniel Iroegbu, Kun Xiang, Yibo Gong, Qing Guan, Wenxiang Wang, Jun Peng, Jianjun Guo, Xun Wu, et al.(ab150074); Alexa Fluor 488-conjugated donkey anti-mouse IgG (H&L) (ab150105); and calcein Acetoxymethyl (ab141420) were procured from Abcam (Cambridge, UK). Antibodies against F4/80 (#70076), cleaved-caspase-3 (9664s),

(9) The Proteome Landscape of Human Placentas for Monochorionic Twins with Selective Intrauterine Growth RestrictionGenomics, proteomics & bioinformaticsApril 29, 2023Meng XL, Yuan PB, Wang XJ, Hang J, Shi XM, Zhao YY, Wei Y.medium was discarded, and the cells were labeled with 2 µM of calcein AM fluorescent dye (Catalog No. ab141420, Abcam) at 37 °C for 20 min. A Leica DMI4000B inverted microscope was used to observe the capillary-like

(10) Dimethyloxalylglycine Attenuates Steroid-Associated Endothelial Progenitor Cell Impairment and Osteonecrosis of the Femoral Head by Regulating the HIF-1α Signaling PathwayBiomedicinesMarch 23, 2023Shao W, Li Z, Wang B, Gong S, Wang P, Song B, Chen Z, Feng Y.density of 1 × 10⁴ cells were then seeded on Matrigel for 18 h. Live cells were stained with calcein (ab141420, Abcam, Cambridge, UK), the tube formation ability of EPCs was assessed under a microscope (Olympus,

(11) Ferroptosis is involved in deoxynivalenol-induced intestinal damage in pigsJournal of Animal Science and BiotechnologyMarch 16, 2023Meng Liu, Lei Zhang, Yixin Mo, Jiahuan Li, Jiacheng Yang, Juan Wang, Niel Alexander Karrow, Hao Wu, Lvhui Sunμg/L DON plus 20 μmol/L DFP (DON+DFP) for 24 h. Then, calcein acetoxymethyl ester (Calcein AM, Abcam, ab141420) were used for assaying the cells viability. The fluorescence intensity was observed and analyzed using

(12) Electrodeposition of chitosan/graphene oxide conduit to enhance peripheral nerve regenerationNeural Regeneration ResearchJanuary 1, 2023Ya-Nan Zheng, Ping Chen, Zi-Yuan Zhao, Fei-Xiang Chen, Zi-Yuan Zhao, Fei-Xiang Chen, Xin-Wei Xiao, Zhi-Yi Zheng, Xin-Wei Chenfurther identified by calcein acetoxymethyl ester (calcein AM) staining (50 µg; Calcein AM Assay Kit, ab141420; Abcam, Cambridge, UK). Briefly, SCs on samples were stained with AM dye for 30 minutes at 37°C. Finally,

(13) Targeted delivery and ROS-responsive release of Resolvin D1 by platelet chimeric liposome ameliorates myocardial ischemia-reperfusion injuryJournal of nanobiotechnologyOctober 20, 2022Weng X, Tan H, Huang Z, Chen J, Zhang N, Wang Q, Li Q, Gao J, Sun D, Yakufu W, et al.680 Fluorescent Cell Labeling Dyes (NEV12000, Perkinelmer). It was then incubated with Calcein AM (ab141420, Abcam) labeled BMDMs, and co-localization was observed under a confocal microscope to assess the efferocytosis

(14) RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancerNature communicationsMay 19, 2022Hall AE, Pohl SÖ, Cammareri P, Aitken S, Younger NT, Raponi M, Billard CV, Carrancio AB, Bastem A, Freile P, et al.Following 72 h incubation at 37 °C, 5% CO2 atmosphere, cells were stained for 1 h with Calcein AM (Abcam, ab141420) and bottom and top fluorescence was read on a Victor2 Multilabel Plate Reader (PerkinElmer) at wavelengths

(15) Generation of individualized immunocompatible endothelial cells from HLA-I-matched human pluripotent stem cellsStem cell research & therapyFebruary 2, 2022Song C, Wang L, Li Q, Liao B, Qiao W, Li Q, Dong N, Li L.HUVEC served as the positive control. The resulting tubes were stained with 6 μM Calcein AM (Abcam, ab141420) at 37 °C for 15–30 min and observed under a fluorescence microscope. Matrigel plug assay for vascular

(16) Alcohol-induced CYP2E1, mitochondrial dynamics and retrograde signaling in human hepatic 3D organoidsFree Radical Biology and MedicineNovember 1, 2020Rajesh Angireddy, Anindya Roy Chowdhury, Jacek Zielonka, Gordon Ruthel, Balaraman Kalyanaraman, Narayan G. Avadhanibackground readings. For live and dead cell imaging, organoids were stained with Calcein-AM (Abcam, cat. no: ab141420) (25 μM final concentration) and propidium iodide (10 μg/ml), and live images were captured out using

(17) Type V Collagen in Scar Tissue Regulates the Size of Scar after Heart InjuryCellAugust 6, 2020Yokota T, McCourt J, Ma F, Ren S, Li S, Kim TH, Kurmangaliyev YZ, Nasiri R, Ahadian S, Nguyen T, et al.by liberase as described later. After digestion, cells were incubated with 10μM Calcein AM (Abcam, ab141420) and flow sorted to identify live cells followed by library preparation. Library was generated by Chromium™

(18) Investigation of Au SAMs Photoclick Derivatization by PM-IRRASLangmuirFebruary 4, 2020Wilson Luo, Sydney M. Legge, Johnny Luo, François Lagugné-Labarthet, Mark S. Workentinpeptide was purchased from Peptides International (RGD-3749-PI). Calcein AM was purchased from Abcam (ab141420). Azides A and B were synthesized according to a literature procedure.29-30 Azide C was synthesized

(19) A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte ActivationMediators of inflammationJanuary 1, 2019Ye Z, Zhang S, Liu Y, Wang S, Zhang J, Huang R.(qRT-PCR) analysis were designed and purchased from GenePharma Co. Ltd. (Shanghai, China). Calcein-AM (ab141420), mouse interleukin- (IL-) 6 (ab100712), TNF-α (100747), and IL-1β (ab100704) ELISA Kits and the cholesterol

(20) CDK4/6 dual inhibitor abemaciclib demonstrates compelling preclinical activity against esophageal adenocarcinoma: a novel therapeutic option for a deadly diseaseOncotargetNovember 21, 2017Juliann E. Kosovec, Ali H. Zaidi, Ashten N. Omstead, Daisuke Matsui, Mark J. Biedka, Erin J. Cox, Patrick T. Campbell, Robert W.W. Biederman, Ronan J. Kelly, Blair A. Jobe

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Calcein AM, fluorescent dye for cell viability from Abcam

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Calcein AM, fluorescent dye for cell viability from Abcam

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