CCCP, Mitochondrial oxidative phosphorylation uncoupler from Abcam

Product Specs
ItemCCCP, Mitochondrial oxidative phosphorylation uncoupler
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab141229
Quantity500 mg
Purity0.99
TypeChemical
Molecule Name(3-Chlorophenyl)hydrazonomalononitrile

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Metallothionein loss in cancer cells contributes to increased mutations through defective DNA repair and metabolic imbalancebioRxivJuly 7, 2026Mirna Mina-Abouda, Amy C Rees, Della Evans, Evan Villamor, George Fullbright, Heather R Ghent, Madison A Clark, Wendy Y Zhang, Isabel Koehler, Isabella Berry, et al.37°C. Control samples were treated with 100 μM carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (Abcam Ab141229), a mitochondrial uncoupler. Cells were trypsinized and centrifuged at 1,000 × g for 2 minutes. Cell

(2) DNA fragmentation factor B suppresses interferon to enable cancer persister cell regrowthNature Cell BiologyNovember 17, 2025August F. Williams, David A. G. Gervasio, Claire E. Turkal, Anna E. Stuhlfire, Michael X. Wang, Brandon E. Mauch, Rhea Plawat, Ariel H. Nguyen, Michelle H. Paw, Mehrshad Hairani, et al.purchased from Tonbo Biosciences. Carbonyl cyanide m-chlorophenyl hydrazone was purchased from Abcam (ab141229). BioTracker NucView 530 Red Caspase 3 Dye (PBS) (SCT105) and cytochalasin B (C2743) were purchased

(3) The proteostatic landscape of healthy human oocytesThe EMBO JournalJuly 16, 2025Gabriele Zaffagnini, Miquel Solé, Juan Manuel Duran, Nikolaos P Polyzos, Elvan Böke,Thermo Fisher,T669 Me4Bpy,Bio-Techne,I-190-050 Verapamil,Spirochrome,n/a Bafilomycin A1,Abcam,ab120497 MG-132,Merck Millipore,474790 CCCP,Abcam,ab141229 Proteostat,Enzo,ENZ-51035-K100 Software,Software,Software FIJI,ImageJ.net,2.14.04/1.54f Prism 10,GraphPad,10.4.2 Reagents

(4) Phosphorylation of Optineurin by protein kinase D regulates Parkin-dependent mitophagyiScienceDecember 20, 2024Weil R, Laplantine E, Attailia M, Oudin A, Curic S, Yokota A, Banide E, Génin P.A1,Sigma-Aldrich,Cat#A8674 2-[2-(3-Chlorophenyl)hydrazinylyidene] propanedinitrile (CCCP),Abcam,Cat#ab141229 Phorbol 12-myristate 13-acetate (PMA),Sigma-Aldrich,Cat#P8139 Ionomycine,Sigma-Aldrich,Cat#I9657 Staurosporine,Sigma-Aldrich,Cat#S5921 Hydrogen peroxide solution

(5) Mitochondrial translocase TOMM22 is overexpressed in pancreatic cancer and promotes aggressive growth by modulating mitochondrial protein import and functionMol Cancer ResFebruary 1, 2024Mary Oluwadamilola Haastrup, Kunwar Somesh Vikramdeo, Shashi Anand, Mohammad Aslam Khan, James Elliot Carter, Seema Singh, Ajay Pratap Singh, Santanu Dasguptawell of a 96-well plate. At 48 hours, 50μM Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (Abcam, #ab141229) was added to the media, which was added to the control wells as a positive control. After 15 mins of

(6) Mitochondrial Translocase TOMM22 Is Overexpressed in Pancreatic Cancer and Promotes Aggressive Growth by Modulating Mitochondrial Protein Import and FunctionMolecular Cancer ResearchFebruary 1, 2024Mary Oluwadamilola Haastrup, Kunwar Somesh Vikramdeo, Shashi Anand, Mohammad Aslam Khan, James Elliot Carter, Seema Singh, Ajay Pratap Singh, Santanu Dasguptawell of a 96-well plate. At 48 hours, 50μM Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (Abcam, #ab141229) was added to the media, which was added to the control wells as a positive control. After 15 mins of

(7) Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasisAutophagyJuly 1, 2023Jun YW, Lee S, Ban BK, Lee JA, Gao FB.penicillin-streptomycin in an incubator with 5% CO2 at 37°C. Plated U2OS cells were treated with CCCP (20 μM; Abcam, ab141229) for 2 h in the absence or presence of CHMP4B knockdown and were fixed by first removing half the plate

(8) Dual Mode of Mitochondrial ROS Action during Reprogramming to PluripotencyInternational journal of molecular sciencesSeptember 18, 2022Skvortsova EV, Nazarov IB, Tomilin AN, Sinenko SA.Tocris Bioscience, Bristol, UK), and a protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP, ab141229, Abcam, Cambridge, UK). Media was changed every day, and on the 3rd day, cells were trypsinized and

(9) Low mitochondrial DNA copy number induces chemotherapy resistance via epithelial-mesenchymal transition by DNA methylation in esophageal squamous cancer cellsJournal of Translational MedicineAugust 29, 2022Yuto Kubo et al.the epithelial–mesenchymal transition The uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP; ab141229, Abcam, Cambridge, UK), which is a compound that inhibits the conjugation of both reactions in oxidative

(10) Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex INatureJuly 1, 2022Rodríguez-Nuevo A, Torres-Sanchez A, Duran JM, De Guirior C, Martínez-Zamora MA, Böke E.N,N′-dicyclohexylcarbodiimide (DCCD) (Sigma, D80002) and 30 µM carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (Abcam, ab141229). Survival was assessed by counting the number of oocytes with intact morphology before and after treatments.

(11) Asteltoxin inhibits extracellular vesicle production through AMPK/mTOR-mediated activation of lysosome functionScientific reportsApril 23, 2022Mitani F, Lin J, Sakamoto T, Uehara R, Hikita T, Yoshida T, Setiawan A, Arai M, Oneyama C.cultured at 37 °C in a humidified 5% CO2 chamber. Chemicals Chemicals were obtained commercially: CCCP (ab141229) from Abcam (Cambridge, UK), rapamycin (AG-CN2-0025-M001) and bafilomycin A1 (BVT-0252) from Adipogen

(12) Psiadin and plectranthone selectively inhibit colorectal carcinoma cells proliferation via modulating cyclins signaling and apoptotic pathwaysPLOS ONEJune 4, 2021Khaled Y. Orabi, Mohamed S. Abaza, Yunus A. Luqmani, Rajaa Al-Attiyahinterest. Experimental conditions were standardized using the mitochondrial uncoupler CCCP from Abcam (ab141229) as a positive control. NFκB DNA binding activity Colorectal cancer cells were plated at 2.5 × 105

(13) SUMOylation of mitofusins: A potential mechanism for perinuclear mitochondrial congression in cells treated with mitochondrial stressorsBiochimica et biophysica acta. Molecular basis of diseaseJune 1, 2021Kim C, Juncker M, Reed R, Haas A, Guidry J, Matunis M, Yang WC, Schwartzenburg J, Desai S.Healthcare, Chicago, IL. Flag-antibody is from Sigma, St. Louis, MO (Cat#F3165). CCCP is from Abcam (Cat#ab141229), and MG132 (Cat # I-130) from Boston Biochemicals, Cambridge, MA. Human Flag-tagged Mfn1 (Cat#: RC207184)

(14) Functional role and molecular mechanisms underlying prohibitin 2 in platelet mitophagy and activationMolecular medicine reportsMay 1, 2021Hu LL, Zou K, Chen Y, Wu LJ, Cao J, Xiong XY, Wang L, Cheng XS, Xiao QZ, Yang RQ.(negative control; NC), 10, 20, 50, 70 or 100 µM carbonyl cyanide 3-chlorophenylhydrazone (CCCP; cat. no. ab141229; Abcam), a mitochondrial oxidative phosphorylation uncoupler (16,17). CCCP was diluted using DMSO (Sigma-Aldrich;

(15) Unique metabolic phenotype and its transition during maturation of juvenile male germ cellsFASEB journal : official publication of the Federation of American Societies for Experimental BiologyMay 1, 2021Voigt AL, Kondro DA, Powell D, Valli-Pulaski H, Ungrin M, Stukenborg JB, Klein C, Lewis IA, Orwig KE, Dobrinski I.Canada, #BML-CM111), mitochondrial uncoupling compound FCCP (4 μM; 2 μM for germ cell comparison; abcam, #ab141229), and respiratory chain inhibitor antimycin A (Abcam, #A8674) and rotenone (Enzo Life Sciences, #ALX-350-360)

(16) Mitochondrial Targeting of the Enteropathogenic Escherichia coli Map Triggers Calcium Mobilization, ADAM10-MAP Kinase Signaling, and Host Cell ApoptosismBioOctober 27, 2020Rachana Pattani Ramachandran, Chaya Spiegel, Yael Keren, Tsafi Danieli, Naomi Melamed-Book, Ritesh Ranjan Pal, Efrat Zlotkin-Rivkin, Ilan Rosenshine, Benjamin Aroetiexposed to EPEC strains or the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP; 20 ␮M) (ab141229; Abcam) for 30 min at 37°C in a CO2 incubator. Cells were then immediately subjected to time-lapse confocal

(17) SHIP2 inhibition alters redox-induced PI3K/AKT and MAP kinase pathways via PTEN over-activation in cervical cancer cellsFEBS open bioOctober 1, 2020Azzi A.to control untreated cells. HeLa cells treated with carbonyl cyanide m‐chlorophenyl hydrazone 20 µm (ab141229; Abcam) were used as a control. Statistical analysis Statistical analyses were performed using graphpad

(18) Impaired mitochondrial-endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson's diseaseHuman molecular geneticsMay 28, 2020Berenguer-Escuder C, Grossmann D, Antony P, Arena G, Wasner K, Massart F, Jarazo J, Walter J, Schwamborn JC, Grünewald A, et al.was performed at day 30 of neuronal differentiation. Mitophagy was induced with 25 μM CCCP (Abcam, ab141229) for 24 h. Images were obtained on an Opera QEHS confocal spinning disk microscope (Perkin Elmer) with

(19) Circulating Tumor Cell Clustering Shapes DNA Methylation to Enable Metastasis SeedingCellJanuary 10, 2019Gkountela S, Castro-Giner F, Szczerba BM, Vetter M, Landin J, Scherrer R, Krol I, Scheidmann MC, Beisel C, Stirnimann CU, et al.CCCP treatment and Intracellular Ca2+ measurement FCCP (Abcam, Cat# ab120081) and CCCP (Abcam, Cat# ab141229) were reconstituted in DMSO at 100 mM concentration and further diluted in CTC medium to the indicated

(20) Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality ControlCellJanuary 1, 0001"Yau RG, Doerner K, Castellanos ER, Haakonsen DL, Werner A, Wang N, Yang XW, Martinez-Martin N, Matsumoto ML, Dixit VM, et al."Dithiothreitol (DTT),Invitrogen,Cat#15508-013 "N2,N4-Dibenzylquinazolinine-2,4-diamine (DBeQ)",Sigma-Aldrich,Cat#SML0031-5MG Carbonyl cyanide 3-chlorophenylhydrazone (CCCP),Abcam,Cat#ab141229 Doxorubicin,Sigma-Aldrich,Cat# D1515-10MG Chloroquine,Thermo Fisher Scientific,Cat#ICN19391910 Tunicamycin,Sigma-Aldrich,Cat#T7765-1MG Homoharringtonine,LKT

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CCCP, Mitochondrial oxidative phosphorylation uncoupler from Abcam

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CCCP, Mitochondrial oxidative phosphorylation uncoupler from Abcam

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