PMSF, protease inhibitor from Abcam

Product Specs
ItemPMSF, protease inhibitor
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab141032
Quantity10 g, 100 g
Purity0.99
TypeInhibitor, inhibitor
Molecule NamePhenylmethylsulfonyl fluoride

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot studyFront ImmunolMarch 16, 2026Lena Schröder Alvarez, Iván Conejeros, Gabriel Espinosa, Constanza Salinas-Varas, Benjamin Ott, Markus Weigel, Can Imirzalioglu, Moritz Fritzenwanker, Anita C. Windhorst, Torsten Hain, et al.cocktail (#P8340, Sigma-Aldrich, 1:200), 10 µL orthovanadate (#ab120386, Abcam, 1 mM) and 5 µL PMSF (#ab141032, Abcam, 1 mM). Samples were stored at -20 °C (urine) and -80 °C (blood) until further analysis. For

(2) Glycolysis reprogramming in CAFs promotes oxaliplatin resistance in pancreatic cancer through circABCC4 mediated PKM2 nuclear translocationCell death & diseaseFebruary 23, 2025He R, Hu C, Yuan Y, Li T, Tian Q, Huang T, Lin Q, Zheng S, Chen C, Fu Z, et al.The shRNA sequences are included in Table S2. Western blot analysis Add protease inhibitor (Abcam, ab141032) to RIPA buffer (Thermo Fisher Scientific, 89900) and use it to extract total proteins from cells. According

(3) Astrocyte Dysfunction Reflected in Ischemia-Induced Astrocyte-Derived Extracellular Vesicles: A Pilot Study on Acute Ischemic Stroke PatientsInternational Journal of Molecular SciencesNovember 20, 2024Timea Forró, Doina Ramona Manu, Lucian Barbu-Tudoran, Rodica Bălașavolume of ice-cold protease inhibitor phenylmethylsulfonyl fluoride (Abcam, Cambridge, UK, cat. no. ab141032)-enriched RIPA buffer (Abcam, Cambridge, UK, cat. no. ab156034), the protein amount was measured by

(4) Extracellular Vesicle-Based Characterization of Stem Cell Phenotype in GlioblastomasCureusSeptember 28, 2024Georgiana M Serban, Manu Doina, Rodica Balasa, Adrian F Balasawere lysed with an equal volume of ice-cold phenylmethylsulfonyl fluoride (PMSF, Abcam, catalog no. ab141032) enriched RIPA buffer (Abcam, Cambridge, UK, catalog no. ab156034). After one-step centrifugation at

(5) Protocol for inducing severe Scn2a insufficiency in mice by intracerebroventricular antisense oligonucleotide injectionSTAR ProtocolsJune 21, 2024Melody Li, Bernd Kuhn,Thermo Fisher Scientific,Hs99999905_m1 TRIzol,Invitrogen,15596026 RIPA buffer,Nacalai,16488-34 Protease inhibitor,Abcam,Ab141032 Bradford solution,Bio-Rad,500-0006 Pierce ECL western blotting substrate,Thermo Fisher Scientific,32106 4%–15% Mini-PROTEAN

(6) Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal musclePflügers Archiv - European Journal of PhysiologyJune 1, 2024Ed Maunder, Andrew King, Jeffrey A. Rothschild, Matthew J. Brick, Warren B. Leigh, Christopher P. Hedges, Troy L. Merry, Andrew E. KildingRIPA buffer (20 µL.mg−1 muscle) made with protease inhibitor cocktail (ab65621, Abcam®, 20 μL.mL−1) and PMSF (ab141032, Abcam®, 5 μL.mL−1), and PBS (20 μL.mg−1). Samples were then ground manually and thoroughly using a

(7) GFAP as Astrocyte-Derived Extracellular Vesicle Cargo in Acute Ischemic Stroke Patients—A Pilot StudyInternational Journal of Molecular SciencesMay 24, 2024Timea Forró, Doina Ramona Manu, Ovidiu-Lucian Băjenaru, Rodica BălașaUK, cat. no. ab156034) containing protease inhibitor phenylmethylsulfonyl fluoride (Abcam, cat. no. ab141032). After lysis, the protein amount was measured by a method based on the reaction of proteins with benzethonium

(8) Toxoplasma gondii infection-induced host cellular DNA damage is strain-dependent and leads to the activation of the ATM-dependent homologous recombination pathwayFront Cell Infect MicrobiolMarch 8, 2024Lisbeth Rojas-Barón, Carlos Hermosilla, Anja Taubert, Zahady D. Velásquezinhibitor (Abcam ab120386) and 1 mM phenylmethylsulphonyl fluoride, a serine protease inhibitor (Abcam ab141032). Protein extracts were sonicated for five cycles of 20 s sonication and 20 s resting and then centrifuged

(9) A single cell atlas of circulating immune cells involved in diabetic retinopathyiScienceFebruary 16, 2024Dan Liao, Wei Fan, Na Li, Ruonan Li, Xiaotang Wang, Jiangyi Liu, Hong Wang, Shengping Houextracted from the mixture of lysate buffer (Beyotime, P0013B, China) and protease inhibitor (Abcam, ab141032, UK). The protein concentration was measured by a BCA kit (Beyotime, P0010S, China). The same amount

(10) Toxoplasma gondii Me49 and NED strains arrest host cell cycle progression and alter chromosome segregation in a strain-independent mannerFrontiers in microbiologyJanuary 1, 2024Rojas-Barón L, Hermosilla C, Taubert A, Velásquez ZD.inhibitor (Abcam, ab120386), and 1 mM phenylmethylsulphonyl fluoride, a serine protease inhibitor (Abcam, ab141032). Protein extracts were sonicated for five cycles of 20 s sonication and 20 s resting and then centrifuged

(11) Scn2a insufficiency alters spontaneous neuronal Ca2+ activity in somatosensory cortex during wakefulnessiScienceNovember 17, 2023Melody Li, Mohamed Eltabbal, Hoang-Dai Tran, Bernd KuhnNonidet P-40 Substitute, 0.5% sodium deoxycholate, 0.1% SDS with protease inhibitor added (Abcam, cat# ab141032) and kept in 4°C for 30 min. The lysates were centrifuged at 21,000 rpm for 30 min and supernatants

(12) Fluorofenidone alleviates liver fibrosis by inhibiting hepatic stellate cell autophagy via the TGF-β1/Smad pathway: implications for liver cancerPeerJSeptember 28, 2023Xiongqun Peng, Huixiang Yang, Lijian Tao, Jingni Xiao, Ya Zeng, Yueming Shen, Xueke Yu, Fei Zhu, Jiao Qinlysed with RIPA buffer (#ab156034, Abcam, Cambridge, UK) containing a phosphatase inhibitor and PMSF (#ab141032, Abcam, Cambridge, UK). Protein samples were denatured and separated by 10–15% SDS-polyacrylamide gel

(13) Enlargement of the Axial Length and Altered Ultrastructural Features of the Sclera in a Mutant Lumican Transgenic Mouse ModelPLOS ONEOctober 6, 2016Yanzheng Song, Fengju Zhang, Yanyan Zhao, Mingshen Sun, Jun Tao, Yanchuang Liang, Ling Ma, Yanqiu Yu, Jianhua Wang, Junfeng Haoscleral tissue was homogenized and solubilized in ice-cold PBS containing PMSF protease inhibitor (Abcam/ab141032, Abcam Inc., Cambridge, MA). The total protein concentration was determined using a BCA Protein Assay Kit

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PMSF, protease inhibitor from Abcam

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PMSF, protease inhibitor from Abcam

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