DAPI from Abcam

Product Specs
ItemDAPI
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab285390
Molecule NameDAPI
TypeChemical
Quantity5 mg, 25 mg

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Abcam

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(1) Clinical Significance for risk stratification of papillary thyroid cancer by TUMORFISHER circulating tumor cells techniqueClinical Cancer ResearchApril 1, 2026Liehao Jiang, Xuejie Li, Tianyi Ma, Pingping Zeng, Zhuo Tan, Chuanming Zheng, Jiajie Xu, Lizhuo Zhang, Yaweng Guo, Juyong Liang, et al.(BioLegend, #324203) or cytokeratin (CK) mix (CKmix; Abcam, #ab26448), followed by DAPI staining (Abcam, #ab285390). Flow cytometry (FCM; BD Accuri C6 and FlowJo) and fluorescence microscopy (Olympus IX73 and ImageJ)

(2) Protease-mediated PRC1 dissociation promotes H2AK119ub remodeling during stress responsesThe EMBO JournalMarch 11, 2026Wei Cui, Qingyang Li, Jinsong Wei, Ting Zhou, Haozhe Zhu, Delai Huang, Shuai Wang, Jianan Gao, Ru Zhou, Zeyu Sun, et al.chloride, anhydrous",Sangon Biotech,Cat# 233-140-8 Glycerol,Sangon Biotech,Cat# 231-791-2 DAPI,Abcam,Cat# ab285390 Alexa Fluor-488,Abcam,Cat# ab150077 Alexa Fluor-647,Abcam,Cat# ab150115 β-Mercaptoethanol,Febio science,Cat#

(3) The TRAF6/SPP1 axis participates in osteoarthritis progression through regulating the catabolism and anabolism of cartilage matrixScientific ReportsMarch 4, 2026Jiapei Yao, Jiliu Huang, Chongrui Li, JingJing Shang, Xiaolong Lin, Xindie Zhou, Yaojun Lu(PA5-95599, Invitrogen, USA), β-actin (ab8226, Abcam, UK), and 4′−6-diamidino-2-phenylindole (DAPI) (ab285390, Abcam, UK), all sourced from Abcam. Additional reagents included MMP13 (18165-1-AP, Proteintech, USA),

(4) Modulation of Macrophage ferroptosis under the guide of infrared thermography promotes the healing of pressure injuriesJournal of Advanced ResearchFebruary 1, 2026Xiaoqiong Jiang, Xuanlong Zhang, Huiming Deng, Lulu Lin, Yu Wang, Yuqi Wang, Jiayi Huang, Ningning Yang, Shi Xu, Jian Wang, et al.sections were treated with secondary antibodies for 1 h at 37 °C and then stained with DAPI (Abcam, ab285390) solution. Positive cells were captured using a fluorescence microscope. The expression levels of iNOS,

(5) Rochelle salt-based biodegradable piezoelectric devices for nerve regeneration and intestinal motility monitoringNature CommunicationsJanuary 29, 2026Fanqi Dai, Haofeng Cheng, Hui Qi, Honglai Cai, Abdul Aziz, Tianqi Su, Yanjun Guan, Yuhui Cui, Jingwei Meng, Handi Deng, et al.ab150117, Abcam), was incubated at room temperature for 2 h. Nuclei were stained with DAPI (1:5000, ab285390, Abcam) for 15 min. Images were acquired using a confocal panoramic scanner (3DHISTECH, Panoramic Confocal,

(6) Ginkgolide C alleviates atherosclerosis via activating Nrf2 to inhibit ROS‑dependent NLRP3 inflammasome activationInternational Journal of Molecular MedicineJanuary 27, 2026Rui Zhangpurchased from Abcam. Nuclear visualization employed DAPI staining (0.1 μg/ml PBS; 5 min; cat. no. ab285390; Abcam). Confocal microscopy (Leica TCS SP8, 40x oil lens, NA 1.30) captured images using LAS X 3.7.4

(7) AAV-DJ–Mediated MYOC Silencing as a Gene Therapy Approach for Myocilin-Associated GlaucomaInvestigative Ophthalmology & Visual ScienceJanuary 22, 2026Yue Wan, Xiangxiang Liu, Xuejing Yan, Shen Wu, Yufei Teng, Luyi Han, Jingxue Zhang, Ningli Wang(1:1000, ab150077; Abcam). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:10,000, ab285390; Abcam). Confocal images were acquired using a Leica DMI6000 B inverted microscope (Leica Microsystems,

(8) Berberine Alleviates Shigella-Induced Dysentery by Regulating Intestinal Barrier and Inflammatory ResponsesInternational Journal of Molecular SciencesJanuary 21, 2026Jinwen Ding, Yu Zhang, Xinyu Fan, Yanxing Han, Yuan Lin(1:1000, CST91131) primary antibodies overnight at 4 °C. And then the nucleus was stained with DAPI (ab285390, Abcam, Cambridge, MA, USA). Images were collected on Leica TCS SPS microscope (Wetzlar, Germany). 4.7.

(9) Regulation of Periodontal Ligament Autophagy by Super-Activated Platelet Lysate in a Rat Maxillary Expansion ModelDrug Design, Development and TherapyJanuary 14, 2026Yang Liu, Jianan Li, Huiying Lu, Xiaoyao Liu, Yang Li, Yi Zhang, Peijun Wang(ab192890, Abcam), along with corresponding secondary antibodies (ab150116/ab150073, Abcam) and DAPI (ab285390, Abcam) for nuclear counterstaining. All images were observed and captured using a Leica DM3000 LED

(10) PCID2 is essential for spermatogonial differentiation by regulating alternative splicingCellular and Molecular Life SciencesJanuary 13, 2026Feiyin Zhu, Ying Zhang, Yu Xi, Chenjia Gong, Yanlin Tang, Yidong Chen, Liying Yan, Jie Qiao, Qiang Liuslices were incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, Abcam, ab285390) to stain the nuclei at room temperature for 1 h. The cells were then washed three times with PBST.

(11) YAP1 inhibition protects retinal vascular endothelial cells under high glucose by inhibiting autophagyOpen Life SciDecember 24, 2025Yu-Shan Li, Wen-Qiang Liu, Hong-Dan Yu, Zhong-Fu Zuo, Xue-Zheng Liudilution at 37°C. Post-incubation, the slides were treated with 4′,6-diamidino-2-phenylindole (DAPI) (ab285390) for 5 min in the dark, and subsequently, images were captured using a fluorescence microscope (IX81,

(12) The Neuroprotective Effect of 4-Octyl Itaconate on Acute Period of Experimental Autoimmune NeuritisInflammationDecember 17, 2025Lin-Jie Zhang, Ning Zhao, Jia Li, Hui Zhai, Jie Wu, Li YangiNOS, 1:200, Abcam, USA, cat#ab178945), fluorescent secondary antibodies, and DAPI (Abcam, USA, cat#ab285390), then observed by fluorescence microscopy (Olympus, Japan). MDA, SOD, ROS Detection MDA, SOD and

(13) Preparation of human serum albumin-doxorubicin modified carbon nanotubes mediated combined photothermal and chemotherapeutic strategies in melanoma applicationJ Mater Sci Mater MedDecember 5, 2025Xuechen Yin, Yajiang Yuan, Jianing LiuFluor®488 goat anti-rabbit IgG was purchased from Invitrogen (A21206). DAPI was purchased from Abcam (ab285390). B16 melanoma F10 (B16F10) cells were obtained from ATCC. DMEM was purchased from HyClone (SH30243.01).

(14) MIF/CD74 axis regulates alveolar epithelial type II cell apoptosis, autophagy, and transdifferentiation in bronchopulmonary dysplasia via the TGFβ1/SMAD4 pathwayRespiratory ResearchNovember 25, 2025Mengyao Fan, Shaohua Wang, Zhenzhuang Zou, Jian Deng(Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)) for 1 h at room temperature. After counterstaining with DAPI (Abcam, ab285390), coverslips were mounted onto glass slides. Visualization was performed using a fluorescence microscope

(15) Establishment of a new method for detection of TROP2-positive circulating tumor cells in breast cancerBMC CancerNovember 21, 2025Anqi Wang, Pingping Zeng, Tianyi Ma, Haoyuan Shi, Ruifang Li, Hang Xu, Yinghua Feng, Qiongming Liu, Mengting Wang, Tingmei Chen, et al.was Goat Anti-Mouse IgG H&L/Cy5 (Bioss, BA04296728). Nuclei were stained with DAPI staining (Abcam, ab285390). TROP2 expression was detected using an ultrasensitive nano-flow cytometer (YYL-NXT) (Attune NxT).

(16) Lactate-mediated histone lactylation promotes melanoma angiogenesis via IL-33/ST2 axisCell Death DisOctober 6, 2025Mao Zhao, Yuxuan Qian, Lin He, Taoxin Peng, Hanbin Wang, Xiangxu Wang, Linhan Jiang, Jinrong Fan, Hengxiang Zhang, Di Qu, et al.antibodies: Cy3 (1:1000, ab6939, Abcam, UK), Alexa Fluor 488 (1:1000, ab150077, Abcam, UK), and DAPI (1:1000, ab285390, Abcam, UK). Fluorescence imaging was conducted by laser scanning confocal microscope (LSM 980, Zeiss,

(17) Role of Esketamine in Attenuating Neuroinflammation and Improving Postoperative Cognitive Function via Autophagy Activation Through PARP1 TargetingPhysiol ResOctober 1, 2025Chendi ZHAO, Jiaxin LIU, Shuang ZHAO, Peng LIU, Zhao LI, Yongle LI, Rui DONG, Xiu-Li WANGIgG-FITC labeled, goat anti-mouse IgG-FITC labeled, and DAPI, were all sourced from Abcam (Ab7086, Ab6785, Ab285390). The RFect plasmid DNA transfection kit was obtained from Changzhou Bio-Generating Biotechnology Corp

(18) Obesity-Induced Loss of Function of Bone Marrow Mesenchymal Stromal Cells Is Linked to Cellular Stress and Irreversible at Advanced StagesJ Cell Mol MedAugust 7, 2025Ece Gizem Polat, Mehmet Emin Şeker, Burcu Pervin, Barış Ulum, Fatima Aerts-Kaya(Thermofisher Scientific, A16024). Wells were counterstained with 5 μg/mL 4′,6‐Diamidino‐2‐phenylindole (DAPI, Abcam, ab285390) for 5 min. Pictures were taken using an Olympus IX‐73 fluorescent microscope and assessed using free

(19) Paternal Exposure to Methylphenidate Induces Poor-Quality Blastocyst and Epigenetic ChangesMol Reprod DevMay 23, 2025Ana Clara da Costa Nunes Gomes, Laura Eduarda S. C. Pagliari, Taiza Stumpp, Vanessa Vendraminiwere washed twice in 1% BSA (20 min each) and then stained with 4′,6′‐diamino‐2‐phenyl‐indole (1:1000; ab285390, Abcam) to the identification of nuclei, for 20 min. The blastocysts were then mounted on a slide with

(20) Inhibition of TLR4 mitigates sensorineural hearing loss resulting from cochlear inflammationMol MedMay 5, 2025Jintao Lou, Fan Wu, Wei Liu, Rui Hu, Wuhui He, Yisi Feng, Yan Huang, Jia Guo, Jingman Deng, Zhen Zhao, et al.2 h at room temperature. Finally, the samples were washed three times with PBS, mounted with DAPI (ab285390, Abcam, USA), and imaged using a semi-automatic inverted fluorescence microscope (Olympus IX73, Japan). Cytokine

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