DAPI Staining Solution from Miltenyi Biotec

(1) Patient induced pluripotent stem cells identify specificities of a reticular pseudodrusen phenotype in age-related macular degenerationGenome MedMay 10, 2026Jenna C. Hall, Kavitha Krishna Sudhakar, Maciej Daniszewski, Anne Senabouth, Carla J. Abbott, Helena H. Liang, Himeesh Kumar, Grace E. Lidgerwood, Mehdi Mirzaei, Jessica Yw Ma, et al.suspension containing RPE cells and bound FITC-POS. RPE cells were incubated with DAPI (0.1 µg/mL, Miltenyi, 130–111–570), passed through a 35 µm cell strainer, then analysed on a Becton Dickinson FACS Aria III flow cytometer.

(2) Receptor-guided AAV Tropism Engineering via MATCHbioRxivApril 1, 2026Nolan Graham, Satheesh Kumar, Joseph Rainaldi, Seoin Yang, Andrew Portell, Billy Santoso, Prashant Malicentrifuged and resuspended in 1× PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 at 1 × 106 cells/mL and analyzed on the BD LSRFortessa™ Cell Analyzer, gating for live cells

(3) Expansion of bone marrow adipocytes in obese mice leads to PD-L1-driven bone marrow immunosuppression and osteoclastogenesisBone ResMarch 20, 2026Samantha N. Costa, Carolyn Chlebek, Lindsey Gray, Peter Caradonna, Sergey Ryzhov, Clifford J. Rosenand non-viable cells were distinguished using DAPI (to detect dead nucleated cells) (Miltenyi Biotec 130-111-570). Statistical analyses All data were graphed and analyzed using GraphPad Prism 10.4.1 software. Data

(4) Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial EnvironmentJournal of the American Heart AssociationMarch 17, 2026Ria Göttert, Majed Kikhia, Marie‐Louise Herzog, Wen Pan, Alexandra Klaus‐Bergmann, January Weiner, Dieter Beule, Samuel Knauss, Golo Kronenberg, Michael Potente, et al.BioLegend) were used: SYTOX green (Thermo Fisher Scientific, #S7020, 1:1000), DAPI (Miltenyi Biotec, #130‐111‐570, 1:100), CD45‐APC (#103112, 1:100), CD31‐PE/Cy7 (#102523, 1:100), CD45‐PE (#103106, 1:400), CD11b‐APC

(5) Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancerCancer CellMarch 5, 2026Marco Campisi, Tatsuya Osaki, Ian Dryg, Carla Stornante, Jacqueline Wolff, Jason Weirather, Nicholas Weaver, Mubin Tarannum, Ian Gillanders, Alan Bers, et al.room temperature. Nuclei were counterstained with DAPI staining solution (500:1, Miltenyi Biotec, Cat# 130-111-570) for 15 minutes at RT and mounted using EverBrite™ Hardset Mounting Medium with DAPI (Biotium, Cat#

(6) Mutational Signatures and Clonal Hematopoiesis in Intestinal Metaplasia across Countries with Varying Stomach Cancer IncidenceCancer DiscoveryMarch 2, 2026Kie Kyon Huang, Takeshi Hagihara, Benedict Shi Xiang Lian, Zhi Xuan Ong, Shen Kiat Lim, Roxanne Hui Heng Chong, Supriya Srivastava, Jason Xing Kang, May Yin Lee, Angie Lay-Keng Tan, et al.secondary antibodies at room temperature for 1 hour. Finally, the sections were stained with DAPI (cat. #130-111-570, Miltenyi Biotec) for 1 minute before observation. Mutational Signatures We used SigProfiler (RRID:

(7) Mutational Signatures and Clonal Hematopoiesis in Intestinal Metaplasia across Countries with Varying Stomach Cancer IncidenceCancer DiscovJanuary 14, 2026Kie Kyon Huang, Takeshi Hagihara, Benedict Shi Xiang Lian, Zhi Xuan Ong, Shen Kiat Lim, Roxanne Hui Heng Chong, Supriya Srivastava, Jason Xing Kang, May Yin Lee, Angie Lay-Keng Tan, et al.secondary antibodies at room temperature for 1 hour. Finally, the sections were stained with DAPI (cat. #130-111-570, Miltenyi Biotec) for 1 minute before observation. Mutational Signatures We used SigProfiler (RRID:

(8) Modelling EWS::FLI1 protein fluctuations reveal determinants of tumor plasticity in Ewing sarcomaEMBO Molecular MedicineJanuary 3, 2026Veveeyan Suresh, Christoph Hafemeister, Andri Konstantinou, Sarah Grissenberger, Caterina Sturtzel, Martha M Zylka, Florencia Cidre-Aranaz, Andrea Wenninger-Weinzierl, Karla Queiroz, Dorota Kurek, et al.DPBS). DAPI was added at a final concentration of 10 µg/mL (DAPI Staining Solution Miltenyi Biotec, Cat#130-111-570) to exclude dead cells. The fluorescence intensity of mNG was measured using a BD LSRFortessa Cell Analyzer

(9) CAR T cell engineering impacts antigen-independent activation and co-inhibitionMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Christoph Schultheiß, Simon Stücheli, Brenda Besemer, Maja Kadel, Paul Schmidt-Barbo, Andreas Zingg, Natascha Franz, Sarah Adamo, Frank Stenner, Claudia Fischer, et al.half incubated for 24 h. Killing of target cells was assessed by gating for viable (DAPI-negative, #130-111-570, Miltenyi Biotec) CD3−,CD19+,R110+ target NALM-6-R110 cells and by normalizing their percentage after

(10) Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial EnvironmentJ Am Heart AssocNovember 26, 2025Ria Göttert, Majed Kikhia, Marie‐Louise Herzog, Wen Pan, Alexandra Klaus‐Bergmann, January Weiner, Dieter Beule, Samuel Knauss, Golo Kronenberg, Michael Potente, et al.BioLegend) were used: SYTOX green (Thermo Fisher Scientific, #S7020, 1:1000), DAPI (Miltenyi Biotec, #130‐111‐570, 1:100), CD45‐APC (#103112, 1:100), CD31‐PE/Cy7 (#102523, 1:100), CD45‐PE (#103106, 1:400), CD11b‐APC

(11) Somatic gene delivery faithfully recapitulates a molecular spectrum of high-risk sarcomasNat CommunJune 16, 2025Roland Imle, Daniel Blösel, Felix K. F. Kommoss, Sara Placke, Eric Stutheit-Zhao, Christina Blume, Dmitry Lupar, Lukas Schmitt, Claudia Winter, Lena Wagner, et al.Buffer (Miltenyi Biotec, 130-121-565) before nuclear staining was performed with DAPI (Miltenyi Biotec, 130-111-570) diluted 1:10 in running buffer. Slides were washed three times with running buffer and submitted to

(12) Single-cell transcriptomic profiling reveals diversity in human iNKT cells across hematologic tissuesCell ReportsMay 27, 2025Reyka G. Jayasinghe, Derek Hollingsworth, Nathan C. Schedler, Emily Landy, Chaiyaporn Boonchalermvichian, Biki Gupta, Hao Yan, Jeanette Baker, Beruh Dejene, Kenneth I. Weinberg, et al.Cryoprotective Medium,Lonza,Catalog # BW12–132A "4’,6-diamidino-2-phenylindole (DAPI)",Miltenyi,Catalog # 130–111-570 ,, Critical commercial assays,, ,, "Anti-iNKT MicroBeads, human",Miltenyi,Catalog # 130–094-842 LS Columns

(13) Models of Early Resistance to CDK4/6 Inhibitors Unveil Potential Therapeutic Treatment SequencingInt J Mol SciMarch 14, 2025Elisabet Zapatero-Solana, Yan Ding, Nicholas Pulliam, Alfonso de Dios, Maria Jesus Ortiz-Ruiz, María José Lallenarpm, and were then resuspended in 100 μL Annexin buffer 1× with 1:100 DAPI staining solution (Miltenyi 130-111-570). The stained cells were analyzed using VioBlue (V1) laser for DAPI and PE laser (B2) for Annexin V PE

(14) Fusion oncoproteins and cooperating mutations define disease phenotypes in NUP98 -rearranged leukemiamedRxivJanuary 25, 2025Masayuki Umeda, Ryan Hiltenbrand, Nicole L. Michmerhuizen, Juan M. Barajas, Melvin E. Thomas, Bright Arthur, Michael P Walsh, Guangchun Song, Jing Ma, Tamara Westover, et al.washed by FACS buffer, and resuspended in FACS buffer with DAPI Staining Solution (Miltenyi Biotec.,130–111-570, 1:1000 dilution, for Fig.6–7 only), and analyzed by BD LSRFortessa X-20 Cell Analyzer (RRID:SCR_025285,

(15) Interleukin-2 receptor signaling acts as a checkpoint that influences the distribution of regulatory T cell subsetsiScienceDecember 20, 2024Shouse AN, Villarino AV, Malek TR.#130-104-454 and LS Columns, Miltenyi #130-042-401). Cells were resuspended in sorting buffer; DAPI (Miltenyi #130-111-570) was used at a final concentration of 0.1 μg/mL for viability; and sorting was performed using a BD

(16) Venetoclax induces BCL-2–dependent Treg to TH17 plasticity to enhance the antitumor efficacy of anti–PD-1 checkpoint blockadeCancer Immunol ResAugust 1, 2024Rosy Liao, Jocelyn Y. Hsu, Nada S. Aboelella, Joshua A. McKeever, Anika T. Thomas-Toth, Andrew S. Koh, James L. LaBelleflow cytometry). Immediately prior to flow analysis, samples were counterstained with DAPI (Miltenyi Biotec #130-111-570) according to manufacturer’s protocol. Samples were analyzed on the ImageStreamX MKII via Channel 1

(17) Venetoclax Induces BCL-2–Dependent Treg to TH17 Plasticity to Enhance the Antitumor Efficacy of Anti–PD-1 Checkpoint BlockadeCancer Immunology ResearchAugust 1, 2024Rosy Liao, Jocelyn Y. Hsu, Nada S. Aboelella, Joshua A. McKeever, Anika T. Thomas-Toth, Andrew S. Koh, James L. LaBelleflow cytometry). Immediately prior to flow analysis, samples were counterstained with DAPI (Miltenyi Biotec #130-111-570) according to manufacturer’s protocol. Samples were analyzed on the ImageStreamX MKII via Channel 1

(18) Effect of radiochemotherapy on peripheral immune response in glioblastomaCancer Immunology, ImmunotherapyMay 16, 2024Léa Hampe, Susy Daumoine, Emeric Limagne, Nicolas Roussot, François Borsotti, Julie Vincent, Sylvia Ilie, Caroline Truntzer, François Ghiringhelli, Marion Thibaudincondition was labelled with an anti-CD45 antibody APC-Violet750 (A79392, Beckman Coulter) and DAPI (130-111-570, Miltenyi Biotec). Unlabelled cells were then plated on a membrane coated with an IFN-γ-specific capture

(19) Bioluminescence imaging of Cyp1a1-luciferase reporter mice demonstrates prolonged activation of the aryl hydrocarbon receptor in the lungCommunications BiologyApril 10, 2024Nicolas Veland, Hannah J. Gleneadie, Karen E. Brown, Alessandro Sardini, Joaquim Pombo, Andrew Dimond, Vanessa Burns, Karen Sarkisyan, Chris Schiering, Zoe Webster, et al.dilution 1:100) for 1 h at 4 °C. After washing with 1X PBS, samples were stained with DAPI (Cat No. 130-111-570, Miltenyi) to assess cell viability and then analysed by flow cytometry in the BD FACS Aria III system.

(20) Vivaria housing conditions expose sex differences in brain oxidation, microglial activation, and immune system states in aged hAPOE4 miceExperimental Brain ResearchMarch 1, 2024E. M. Reyes-Reyes, J. Brown, M. D. Trial, D. Chinnasamy, J. P. Wiegand, D. Bradford, R. D. Brinton, K. E. Rodgersfollowed by PBS wash. After staining, the cells were resuspended, and DAPI (Miltenyi Biotec; Cat. #130-111-570) was added to exclude nonviable cells. Flow cytometry was used to analyze the results. Flow cytometry

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DAPI Staining Solution from Miltenyi Biotec

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DAPI Staining Solution from Miltenyi Biotec

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