Description
SW-100, a selective histone deacetylase 6 (HDAC6) inhibitor with an IC 50 of 2.3 nM, shows at least 1000-fold selectivity for HDAC6 relative to all other HDAC isozymes. SW-100 displays a significantly improved ability to cross the blood-brain-barrierIn VitroSW-100 (0.01-10 µM; 48 hours) shows obvious increase in the acetylated α-tubulin levels in a dose-dependent manner. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: HEK293 cells Concentration: 0.01, 0.1, 1, 10 µM Incubation Time: 48 hours Result: Showed obvious increase in the acetylated α-tubulin levels in a dose-dependent manner.In VivoSW-100 (20 mg/kg; i.p.; twice a day for two days) ameliorates several memory and learning impairments including novel object recognition, temporal ordering, and coordinate and categorical spatial processing in mouse model of Fragile X syndrome. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 8-10 weeks old C57BL/6 mice ( Fmr1 -/- mice) Dosage: 20 mg/kg Administration: Intraperitoneal injection; twice a day for two days Result: Ameliorated several memory and learning impairments including novel object recognition, temporal ordering, and coordinate and categorical spatial processing in Fmr1 -/- mice。Form:SolidIC50& Target:HDAC1 5.23 µM (IC 50 ) HDAC2 32.8 µM (IC 50 ) HDAC3 29.5 µM (IC 50 ) HDAC4 10.9 µM (IC 50 ) HDAC5 4.07 µM (IC 50 ) HDAC6 2.3 nM (IC 50 ) HDAC7 4.55 µM (IC 50 ) HDAC8 3.72 µM (IC 50 ) HDAC9 3.46 µM (IC 50 ) HDAC10 26.2 µM (IC 50 ) HDAC11 5.72 µM (IC 50 )