Description
Diflapolin is a highly active dual 5-lipoxygenase-activating protein (FLAP) /soluble epoxide hydrolase (sEH) inhibitor with marked anti-inflammatory efficacy and high target selectivity. Diflapolin inhibits 5-LOX product formation in intact human monocytes and neutrophils with IC 50 s of 30 and 170 nM, respectively, and suppressed the activity of isolated sEH ( IC 50 =20 nM).In VivoDiflapolin (1-10 mg/kg; i.p.; 30 min before zymosan injection) exhibits potent anti-inflammatory properties in in-vivo experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Male CD-1 mice (zymosan-induced peritonitis mouse model) Dosage: 1, 3 and 10 mg/kg Administration: I.p.; 30 min before zymosan injection Result: Induced a significant reduction of LTC4 and LTB4 peritoneal levels, starting from the dose of 1 mg/kg and comparable to the effect of MK886