Description
Methotrexate hydrate has been used:as a dihydrofolate (DHFR) inhibitor in sphere forming assay cultureas a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cellsin viability and co-culture assaysPotent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistantPlasmodium vivaxmalaria parasites