Description
InformationOSMI-4 OSMI-4 is an inhibitor of O-GlcNAc transferase (OGT) with EC50 of 3 µM in cells.TargetsOGT (Cell-free assay) 3 µM(EC50)In vitroOSMI-4 reduces O-GlcNAc levels almost completely by 5 µM. A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 is observed in cells treated with OSMI-4. OSMI-4 is a kind of structure-based evolution of small molecule OGT inhibitors with low nanomolar binding affinities. OSMI-4 exhibits a ~3 µM EC50 in cells, making it especially attractive for probing OGT’s complex biology.Cell Research(from reference)Cell lines：HEK293T cells Concentrations：5 µM, 10 µM, 20 µM Incubation Time：2 h, 8 h, 16 h, 24 h