Description
It belongs to the trialkylphosphine class. Tris (2-carboxyethyl) phosphine (TCEP) is very effective in cleaving disulfide bonds in aqueous solution. It dissolves in water and is odorless, unlike other trialkylphosphines (tributylphosphine). It is also less toxic than 2-mercaptoethanol. These advantages make it better than the other reducing agents. Application：Tris (2-carboxyethyl) phosphine (TCEP) can be used in several downstream applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. It prevents oxidation of protein samples, which makes it a useful buffer component as it helps to preserve enzymatic activity. It has been used in the reduction and measurement of glutathione.Tris (2-carboxyethyl) phosphine (TCEP) has also been used:to cleave cysteine residues in a synthetic peptidein reduction buffer for RNA Sequential Probing of Targets (SPOTs) imagingfor the reduction of oligonucleotidesas reducing agent during mitochondrial isolation