Description
DA 3003-2 is a potent and selectively Cdc25 inhibitor. DA 3003-2 shows antiproliferative activity. DA 3003-2 induces cell cycle arrest at the G2/M phase and increases the expression of P-tyr 15 Cdc2. DA 3003-2 has the potential for the research of prostate cancer.In VitroDA 3003-2 (0.3-30 µM; 48 h) shows antiproliferative activity with an IC 50 value of 5 µM in PC-3 cells. DA 3003-2 (5, 10 µM; 24, 1 h) induces cell cycle arrest at G2/M phase and increases the expression of P-tyr 15 Cdc2 in PC-3 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity AssayCell Line: PC-3 cells Concentration: 0.3-30 µM Incubation Time: 48 h Result: Showed antiproliferative efficacy in a dose-dependent manner with an IC 50 value of 5 µM. Cell Cycle AnalysisCell Line: PC-3 cells Concentration: 5, 10 µM Incubation Time: 24 h Result: Induced cell cycle arrest at G2/M phase. Western Blot AnalysisCell Line: PC-3 cells Concentration: 5, 10 µM Incubation Time: 1 h Result: Increased the expression of P-tyr 15 Cdc2.Form:Solid