Description
Col003 is a selective and potent inhibitor of Hsp47 and competitively binds to the collagen binding site on Hsp47 ( IC 50 =1.8 µM). Col003 discourages the interaction of Hsp47 with collagen and inhibits collagen secretion by destabilizing the collagen triple helix. Col003 can be used for the investigation of fibrosisIn VitroCol003 (4.4-40 µM) is cleaved from Col001 (AK-778). Col003 has an inhibitory effect on the interaction of Hsp47 with collagen in Hsp47 KO ?/? MEFs. ?\nCol003 (0.01-100 µM) exhibits dose-dependent effects on the interactions of Hsp47 with collagen. The IC 50 ?value is 1.8 µM for Hsp47. ?\nCol003 (100 µM; 20-60 mins) has an nhibitory effects on collagen secretion and accumulation. It inhibits collagen secretion by wild-type MEFs and the secretion is not abolished completely. ?\nCol003 (100 µM) degrades α,α′-dipyridyl collagen secretion completely by incubation with trypsin at a high temperature (50 °C for 15 min), but it resistant to trypsin digestion at 37 °C. ?. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: Wild-type MEFs Concentration: 100 µM Incubation Time: After 50 °C or 37 °C for 15 min Result: Affected correctly folded triple helix structures and α,α′-dipyridyl on collagen secretion.Form:SolidIC50& Target:HSP47 1.8 µM (IC 50 )