RT Master Mix for qPCR II from MedChemExpress

Product Specs
ItemRT Master Mix for qPCR II
CompanyMedChemExpress
Price $210.00Supplier Page View Company Product Page
Catalog NumberHY-K0510A
Quantity100 rxns
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) Beyond defense: GTR-mediated uptake of glucosinolates drives growth and structural reprogramming in broccoli seedlingsPlant PhysiologyAugust 4, 2026Lorena Albaladejo-Marico, Christa Kanstrup, Christoph Crocoll, Micaela Carvajal, Josip Tomas, Marie Schuler, Arnaud Tatibouët, Hussam Hassan Nour-Eldin, Lucia Yepes-Molinaanalysis cDNA synthesis was carried out using 200 ng of total RNA with the RT Master Mix for qPCR II Kit (HY-K0510A, MedChemExpress, Sollentuna, Sweden), according to the manufacturer's instructions. Quantitative real-time PCR

(2) M2 macrophage-derived exosomes improves secondary lymphedema through cellular mitochondrial homeostasis regulation via the Keap1-Nrf2/mPTP axisFront ImmunolJuly 10, 2026Jinli Ma, Luya Pu, Yundong Zhang, Baiao Wu, Rui Fei, Dongmei Han, Miao Hao, Jianshi DuReverse transcription quantitative PCR Total RNA was extracted and reverse-transcribed into cDNA (MCE, HY-K0510A, USA). Quantitative PCR amplification was performed using SYBR Green qPCR Master Mix (MCE, HY-K0501A,

(3) Loss of KLF15 expression characterizes proximal tubule injury in cisplatin-induced acute kidney injury: A multi-omics studyCurrent Research in ToxicologyMay 19, 2026Lei Chen, Shiying Xie, Heqing Yang, Xiangshen Liu, Shunliang Yang, Dong WangThermo Fisher Scientific, USA). Complementary DNA (cDNA) was synthesized using the RT Master Mix for qPCR II (HY-K0510A, MCE, USA). RT-qPCR was performed on a QuantGene 9600 Real Time PCR System (BIOER, China) using HS Universal

(4) miR-495-3p promotes chronic obstructive pulmonary disease by activating ferroptosis in lung epithelial cells through regulation of the ETS1/GPX4 axisMol Biol RepApril 25, 2026Hongbo Chen, Yungui Tu, Lina Wang, Simeng ZhuRNA was subsequently reverse transcribed into cDNA using a conventional reverse transcription kit (HY-K0510A; MedChemExpress, USA) or a microRNA reverse transcription kit (HY-K0512; MedChemExpress, USA). qPCR

(5) Hypoxia-Inducible Factor-1α Drives Cellular Senescence via Autophagy Activation to Promote Acute Kidney Injury-to-CKD TransitionAmerican Journal of NephrologyMarch 16, 2026Manzhu Zhang, Lingxu Li, Xinyue Mao, Xiaoying Yun, Meiting Wu, Chang Wang, Bing Liextracted using TRIzol RNA isolation reagent (BS258A, Biosharp). cDNA was synthesized with RT Master Mix (HY-K0510A, MCE). mRNA expression was detected using SYBR Green qPCR Master Mix (B21203, Selleck) on a CFX96 Real-Time

(6) BMAL1-GPX3 axis in the choroid plexus mitigates Aβ pathology in an amyloid mouse modelJournal of NeuroinflammationFebruary 6, 2026Fenglin Tang, Yufeng Li, Xue Bai, Zhongmou Zhu, Hongwei Dong, Jianhui Chen, Bo Ye, Meng Yuan, Qilong Wu, Weishan Fu, et al.1. qPCR Total RNA was extracted using a commercial kit (Tiangen, DP451) and reverse transcribed using the MCE HY-K0510A kit. qPCR was performed using 2× Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) on a Roche real-time PCR system

(7) An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue EngineeringBioengineering (Basel)January 26, 2026Juhan Li, Nan Xiang, Lingbin Che, Jianfeng Wu, Dianwen Songsynthesized from total RNA using a reverse transcription kit (Monmouth Junction, NJ, USA, MedChemExpress HY-K0510A). qPCR was performed using SYBR Green Master Mix on a real-time PCR system (USA, Thermo Fisher Scientific,

(8) SYVN1 aggravates esophageal squamous cell carcinoma development by activating NF-κB pathway to facilitate macrophage M2 polarizationJournal of Thoracic DiseaseOctober 31, 2025Kang Wang, Nan Yang, Yuan Qiu, Zhao Huang, Yi Shen, Jing Luowere assessed using a NanoDrop (Thermo Fisher Scientific, Waltham, USA). The RT Master Mix kit (cat#HY-K0510A, MedChemExpress, Monmouth Junction,USA) was adopted for cDNA synthesis. By means of a Faststart SYBR Green Master

(9) Light intensity and sulfur deficiency modulate growth and water dynamics in broccoli plants via aquaporin regulationPlant Biol (Stuttg)October 1, 2025A. Almagro‐Lopez, O. Puma, V. Cirillo, A. Maggio, J. Nicolas‐Espinosa, M. CarvajalExtracted RNA samples were preserved at −80°C until further analysis. The RT Master Mix for qPCR II Kit (HY‐K0510A‐MedChemExpress, Sollentuna, Sweden) was used to synthesize cDNA from 2 μg total RNA, according to the

(10) Essential function of the integrator complex in Kaposi’s sarcoma-associated herpesvirus lytic replicationJournal of VirologySeptember 23, 2025Amy Nguyen, Tianqi Li, Conner Traugot, Kimberly Paulsen, Tiffany S. Nelson, Mingyi Xie, Zhe Masynthesized using the RT Master Mix for real-time quantitative PCR (qPCR) II from MedChemExpress (Cat#HY-K0510A). The housekeeping gene β-Actin was used to normalize transcript levels. An equal volume of supernatants

(11) KSHV miRNAs target STING to evade innate immunity and facilitate KSHV lytic reactivation from latencyCell ReportsJune 24, 2025Kimberly Paulsen, Rosenna Chan, Lauren Gay, Zhe MaTRIzol. Subsequently, cDNA synthesis was performed using a reverse transcription kit from (MedChemExpress HY-K0510A). qPCR reactions were carried out in triplicate using an SYBR Green master mix (APExBIO K1170) on a

(12) Unveiling the Therapeutic Potential of Baicalin in Intervertebral Disc Degeneration: Integrative Bulk and Single-Cell Transcriptome Analysis with Experimental Validation of PANoptosis InhibitionJ Inflamm ResMay 30, 2025Xiaoqiang Wang, Silong Gao, Daqian Zhou, Weiye Cai, Jiale Lv, Zhangchao Wei, Chao Song, Xubin Shan, Zongchao Liuand BCA protein assay kit (71285-M) were obtained from Sigma. Reverse transcription and qPCR kits (HY-K0510A and HY-K0501) were purchased from MedChemExpress. Hematoxylin-eosin (HE) staining kit (G1120) and Annexin V-FITC/PI apoptosis detection kit

(13) Unveiling the Therapeutic Potential of Baicalin in Intervertebral Disc Degeneration: Integrative Bulk and Single-Cell Transcriptome Analysis with Experimental Validation of PANoptosis InhibitionJ Inflamm ResMay 30, 2025Xiaoqiang Wang, Silong Gao, Daqian Zhou, Weiye Cai, Jiale Lv, Zhangchao Wei, Chao Song, Xubin Shan, Zongchao Liuand BCA protein assay kit (71285-M) were obtained from Sigma. Reverse transcription and qPCR kits (HY-K0510A and HY-K0501) were purchased from MedChemExpress. Hematoxylin-eosin (HE) staining kit (G1120) and Annexin V-FITC/PI apoptosis detection kit

(14) Notch1 signaling regulates Sox9 and VEGFA expression and governs BMP2-induced endochondral ossification of mesenchymal stem cellsGenes & diseasesMay 1, 2025Zou J, Du C, Liu S, Zhao P, Gao S, Chen B, Wu X, Huang W, Zhu Z, Liao J.total RNA was reverse transcribed using a reverse transcription reagent kit (RT Master Mix for qPCR, HY-K0510A, MCE). The cDNA was diluted and mixed with SYBR Green qPCR Master Mix (HY–K0501, MCE) and specific primers

(15) The transition between M1 and M2 macrophage phenotypes is associated with the disease status following CD19 CAR-T therapy for B cell lymphoma/leukemiaCell Death DisApril 11, 2025Li Zhao, Fen Yan, Donghai Tang, Wenwen Li, Na An, Chunxiao Ren, Ying Wang, Kailin Xu, Kai Zhaoreverse transcription to cDNA was performed, followed by qPCR using RT Master Mix for qPCR II (Cat: HY-K0510A; MCE) and Magic SYBR Mixture (Cat: CW3008M; CWBIO) according to the manufacturer’s protocols. Melting

(16) Carnosic Acid Directly Targets STING C-Terminal Tail to Improve STING-Mediated Inflammatory DiseasesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2025Mu W, Xu G, Li L, Wen J, Xiu Y, Zhao J, Liu T, Wei Z, Luo W, Yang H, et al.enzyme‐free water. Next, 1 µg of total RNA was reverse transcribed into cDNA using RT Master Mix for qPCR II (HY‐K0510A, MCE). Then, the qPCR was performed in10 µL reaction mixtures containing 25 ng cDNA, 5 µL SYBR Green qPCR Master Mix

(17) The Role of Heat Shock Factor 1 in Preserving Proteomic Integrity During Copper-Induced Cellular ToxicityInternational journal of molecular sciencesOctober 30, 2024Ghai S, Shrestha R, Hegazi A, Boualoy V, Liu SH, Su KH.(P4707) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Additionally, RT Master Mix for qPCR II (HY-K0510A) and SYBR Green qPCR Master Mix (Low ROX) (HY-K0522) were obtained from MCE. 4.2. Cell Culture HEK293T

(18) Conduction and validation of a novel mitotic spindle assembly related signature in hepatocellular carcinoma: prognostic prediction, tumor immune microenvironment and drug susceptibilityFrontiers in GeneticsJuly 19, 2024Zhao Zhang, Yuezhou Zhang, Gangli Hu, Qianxue Wu, Yang Zhou, Fang LuoRE-03111, FOREGENE). Subsequently, the RNA was converted to cDNA using a cDNA synthesis kit (Cat. No.: HY-K0510A, MCE). Quantitative real-time PCR was performed using a Bio-Rad CFX96 instrument with GAPDH as the reference

(19) Adipose transplantation improves metabolism and atherosclerosis but not perivascular adipose tissue abnormality or vascular dysfunction in lipodystrophic Seipin/Apoe null miceAmerican journal of physiology. Cell physiologyMay 1, 2024Meng Z, Liu C, Xu M, Tao Y, Li H, Wang X, Liao J, Wang M.using Tri-reagent (R1100; Solarbio, Beijing, China) and reverse-transcribed into cDNA with an RT kit (HY-K0510A; MedChemExpress, Monmouth Junction, NJ). Quantitative real-time PCR was performed with SYBR Green qPCR

(20) Overexpression of TNFSF11 reduces GPX4 levels and increases sensitivity to ferroptosis inducers in lung adenocarcinomaJournal of Translational MedicineApril 9, 2024Zizhen Li, Wenhua Lu, Feng Yin, Peiting Zeng, Heping Li, Amin Huangtechologies #15596-026). cDNA was synthetized with 0.5–1 µg RNA by using a RT Master Mix for qPCR II (MCE, #HY-K0510A) and then mixed with SYBR-Green Master Mix for amplification by using the Bio Rad CFX96 system (Bio-Rad

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RT Master Mix for qPCR II from MedChemExpress

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RT Master Mix for qPCR II from MedChemExpress

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