H2DCFDA from MedChemExpress

Product Specs
ItemH2DCFDA
CompanyMedChemExpress
Price $20.00Supplier Page View Company Product Page
Catalog NumberHY-D0940
TypeChemical
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Quantity5 mg
Purity99.82%
Research AreaOthers

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(1) Natural molecule potentiates colistin efficacy in vivo via modulating adaptive LPS modifications and ferroptotic-like damagesVirulenceDecember 31, 2026Hui-Hui Zhang, Yi-Dan Cao, Ying-Ying Xie, Yu-Ze Li, Xiao-Na Fan, Qiu-Yue Diao, Yu-Jiao Liang, Li-Ren He, Zi-Xing Zhong, Li-Juan Xia, et al.mL−1) with or without candidate chemical (25 μg mL−1), followed by incubation with the DCFH-DA probe (HY-D0940, MCE, USA) for 3 h. Intracellular reactive oxygen species (ROS) in the treated bacterial cells were

(2) Hierarchical targeting biomimetic nanoparticles directing to lesion site and mitochondria promote neuroregeneration and attenuate inflammation after spinal cord injuryMater Today BioAugust 12, 2026Yu Zhou, Yijie Kong, Zhiquan Yang, Kai Zhang, Runlin Wen, Shiyu Fu, Wanrong Ma, Ge Long, Xing Li, Dingyang Liuinterventions were washed with cold PBS and incubated with H2DCFDA working solution (MedChemExpress, HY-D0940) for 30 min at 37 °C. The cells were immediately washed with PBS, and ROS levels were assessed by fluorescence microscopy

(3) Fisetin alleviates zinc overload-induced intestinal injury by inhibiting ferroptosis and remodeling gut microbiota in weaned pigletsJ Anim Sci BiotechnolAugust 9, 2026Feifei Huang, Yuhang Deng, Mohan Zhou, Huangen Xu, Jie Fengprevious study [23]. For piglet jejunum, frozen sections were prepared and incubated with DCFH-DA (#HY-D0940, MedchemExpress, Monmouth Junction, NJ, USA) at 37 °C for 30 min in the dark. After washing, ROS fluorescence

(4) NF-κB/Lipocalin 2 Signaling Pathway Mitigates the Chemoresistance of BRAFV600E-Mutant Colorectal Cancer to Cisplatin by Promoting FerroptosisCancers (Basel)August 9, 2026Meibao Feng, Xuesong Wu, Li Jiang, Jinyan Huang, Jing Zhang, Pei Chen, Chengdong Changwas incubated with the corresponding cells at a concentration of 30 μM for 24–48 h. H2DCFDA (Cat. No. HY-D0940) and ZVAD-FMK (Cat. No. HY-16658B) were from Med Chem Express (MCE, Hao yuan Bioscience, Shanghai, China).

(5) Integrated computational and in vitro analysis reveals the toxic mechanisms of PFAS in female diminished ovarian reserveSci RepAugust 1, 2026Hengbing Li, Xin Hu, Zizhen Guo, Zhengao Sun36126ES03) membranes were obtained from YEASEN(China). 2’,7’-dichlorofluorescin diacetate (DCFH-DA, HY-D0940) was purchased from MedChemExpress (MCE) (USA). PFOA was dissolved in DMSO to prepare a 200 mM stock

(6) Pyrroloquinoline Quinone Improves Cognitive-Related Behavioral Performance Associated with Enhanced Mitochondrial Bioenergetics in Naturally Aged MiceAntioxidants (Basel)July 24, 2026Yun Yan, Di Deng, Chunxia Tan, Qi Lu, Jiutang Sun, Shaoliang Wu, Zhanhua Jiang, Yibo Li, Tao Lufluorescence intensity. Intracellular ROS levels were assessed using the DCFH-DA fluorescent probe (HY-D0940; MedChemExpress, Monmouth Junction, NJ, USA). Following DCFH-DA incubation, cells were washed to remove

(7) CHAC2-mediated glutathione metabolic reprogramming drives N1 polarization of bone marrow neutrophils and exacerbates inflammatory comorbiditiesInt J Oral SciJuly 9, 2026Yuting Niu, Yuman Li, Shiyu Sun, Chenyu Deng, Fan He, Yiming Chen, Tiansong Xu, Zhewen Hu, Gai Yang, Leran Li, et al.using ThiolTracker Violet (T10095, Thermo Fisher Scientific), and ROS levels were measured using DCFDA (HY-D0940, MedChemExpress). To preserve neutrophil viability and functional status, the entire procedure from

(8) Interferon-elicited lipoprotein metabolism in lung fibroblasts facilitates premetastatic niche formationNature CommunicationsJuly 1, 2026Jian Gao, Yilan Huang, Xiaojun Zhang, Chao Gao, Yiran Zhuang, Zheng Han, Mingyu Xia, Chun Jin, Dingguo Wang, Lingxian Zhang, et al.fluorescence microscope. For ROS detection, 2 × 10⁴ cells were stained with 1 μM H2DCFDA (MCE, Cat# HY-D0940) for 30 min at 37 °C. Afterward, the cells were washed twice with staining buffer, and cellular ROS

(9) Molecular Iodine/PPARγ Interaction in the Invasion and Angiogenesis of Neuroblastoma XenograftsCellsJune 30, 2026Edgar R. Juvera-Avalos, Gustavo Orizaga-Osti, Evangelina Delgado-Gonzalez, Hilda Lomeli, Brenda Anguiano, Carmen Acevesmethanesulfonate salt (Cat. No. A5040-25G) were obtained from Sigma-Aldrich (St. Louis, MO, USA). H2DCFDA (HY-D0940) was obtained from MedChemExpress (Monmouth Junction, NJ, USA). Hydrogen peroxide (Cat. No 56001) was

(10) Vitamin K2 Promotes Mitochondrial Structural and Functional Homeostasis to Ameliorate Alzheimer Pathology by Targeting the EGFR-Ras-ERK Signaling AxisInternational Journal of Molecular SciencesJune 24, 2026Yanan Li, Hanyu Zhao, Jie Wu, Yan Hu, Juhong Pan, Asante Obed Frimpong, Biguo Xie, Wanming Yang, Manman Sun, Wenjun Chen, et al.China). Additionally, tetramethylrhodamine ethyl ester (TMRE) dyes (Cat# HY-D0985A) and H2DCFDA probe (Cat# HY-D0940) were purchased from MCE (Shanghai, China). Color PAGE Gel Rapid Preparation Kit (Cat# PG112); SuperKine™

(11) Tanshinone IIA inhibits epithelial-mesenchymal transition in ovarian cancer by promoting NR3C2 transcriptional activation of ING1iScienceJune 19, 2026Xiaoqin Wang, Duoyi Zhang, Tingting Wang, Xin Li, Jing Zhangabsorbance at 490 nm was read. Cellular reactive oxygen species (ROS) assay Fluorescent probe DCFH-DA (HY-D0940, MedChemExpress) was used to assess ROS levels in SK-OV-3 and OVCAR-3 cells. OV cells were treated with

(12) Fine particulate matter and tobacco product exposure exacerbates metabolic syndrome-related colon cancer via regulating oxidative stress and tumor-associated macrophage interactionsCancer & MetabolismJune 16, 2026Shenghua Zhang, Dongfang Meng, Haibo Zhang, Fei Pan, Yongsheng Gao, Jijin Wangpopulation was determined by 2’,7’-dichlorodihydrofluorescein diacetate (10 µM) labeling (DCFH-DA, #HY-D0940, MedChemExpress). To detect mitochondrial ROS, the MitoSOX Red (#HY-D1055, MedChemExpress, Shanghai,

(13) Biphasic Responses of Porcine Oocytes to Metformin: Concentration-Dependent AMPK Activation and Nrf2-Mediated Antioxidant RegulationAnimals (Basel)June 13, 2026Junyu Wang, Min Li, Yaqi Zhou, Fuyin Fu, Feng Liu, Jinghe Tan, Mingjiu Luo, Shuai Gongspecies (ROS) levels were measured using 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA, HY-D0940, MCE, Monmouth Junction, NJ, USA). Oocytes were incubated with the probe at 37 °C for 10 min in the

(14) Mesoporous catechin hydrogel for enhanced astaxanthin-based inflammatory bowel disease therapyJournal of NanobiotechnologyJune 11, 2026Ningli Guo, Jiayi Shi, Xinyu Zhang, Hui Xu, Tongyao Ji, Fengkai Qiu, Kai Li, Zhenyi Wu, Ji Ma, Qiaoyou Weng, et al.1,3,5-Trimethylbenzene (Cat. #: 108-67-8) was obtained from Aladdin (Shanghai, China). DCFH-DA (Cat. #: HY-D0940), LPS (Cat. #: HY-D1056) and DiR (Cat. #: HY-D1048) were purchased from MedChemExpress (Shanghai, China).

(15) Genotype-environment interaction drives the onset of riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency in carriers of single heterozygous ETFDH variantsCell Communication and SignalingJune 2, 2026Jing Ma, Huiqiu Zhang, Jiaqi Jiao, Menghan Su, Junsen Zhao, Dan Liu, Qi Wen, Jianli Wang, Juan Wang, Weisong Duan, et al.measurement The detection of intracellular ROS levels was performed using the ROS-sensitive probe H2DCFDA (HY-D0940, MCE, USA). Cells were incubated with 10 µM H2DCFDA staining solution in serum-free DMEM in the dark

(16) Combine immune checkpoint indoleamine 2,3-dioxygenase 1 knockdown with ferroptosis inducer Erastin/RSL3 accelerates colorectal cancer cell ferroptosisCytojournalMay 29, 2026Fei Li, Taixi Xu, Yingpei Ling, Jiajia Xu, Xiwen Zhang, Shumei Sunremoved, and the cells were incubated with an appropriate volume of diluted DCFH-DA working solution (HY-D0940, MedChemExpress, USA) at 37°C for 20 min. During incubation, the dishes were gently inverted every 5

(17) Engineered neuronal exosomes mediate α-synuclein clearance to ameliorate Parkinson’s diseaseJournal of NanobiotechnologyMay 23, 2026Lufei Chen, Xiaoling Lin, Mingzhi Fu, Shenglang Chen, Zhexiao Yan, Yuelin Diao, Genyu Chen, Zichen Huang, Yan Sun, Yaxin Lin, et al.mitochondrial function in live cells All live-cell staining assays for cytoplasmic ROS (DCFH-DA, MCE, HY-D0940), mitochondrial ROS (MitoSOX Red, MCE, HY-D1055), mitochondrial membrane potential (JC-1, MCE, HY-15534),

(18) Endothelial NOX4‑driven oxidative stress inhibition reverses HtrA1 deficiency‑induced blood‑brain barrier disruption and cognitive impairmentInt J Mol MedMay 19, 2026Shi-Na Song, Jun-Ying Wu, Mao-Mei Song, Xiao-Feng Li, Jian-Ming Wang, Wen-Hui Jia, Chang-Xin Li, Sui-Yi Xu(1.5×105) were seeded in a 6-well plate and cultured for 24 h at 37°C. DCFH-DA (MedChemExpress; cat. no. HY-D0940) staining was performed when the cell density reached 80%. The culture medium was discarded and the

(19) Gut microbiota-induced perturbation in bile acids alter keratinocyte lipid metabolism via FXR-NQO1 signaling in psoriasisNature CommunicationsMay 18, 2026Panpan Lian, Renwei Lu, Chaode Gu, Junaid Wazir, Wentong Wang, Yangyongyi Zong, Yun Xia, Jie Ni, Huimei Chen, Enrico Petretto, et al.equivalents. Reactive oxygen species (ROS) The ROS levels in HaCaTs were measured by H2DCFDA (MedChemExpress, #HY-D0940), an ROS-sensitive fluorescent probe. HaCaTs were seeded in 12-well plates and treated with IL-17 (Sino,

(20) Staphylococcus epidermidis prevents UV-induced skin aging by suppressing TLR3-mediated senescenceFront ImmunolMay 12, 2026Xinxin Wang, Mengke Wang, Jingya Yang, Wenbo Liu, Haidong Jia, Yuanyuan Chen, Yuping LaiIntracellular reactive oxygen species (ROS) levels were measured using the DCFH-DA probe (MedChemExpress, HY-D0940). Fluorescence was detected with excitation at 488 nm and emission at 525 nm. Long-term UV-induced

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H2DCFDA from MedChemExpress

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