Description
A GPX4 and TrxR1 inhibitor; covalently binds to the GPX4 active site residue Sec46 and allosteric site residue Cys66 but does not inhibit recombinant human GPX4 activity in a cell-free assay; inhibits TrxR1 in a cell-free assay (IC50 = 19.5 µM); inhibits TrxR1 in A549 cells at 0.5-15 µM; decreases GPX4 levels in MDA-MB-231 breast cancer cells; reduces the viability of BT-549 breast cancer cells, an effect that can be enhanced by α-eleostearic acid; induces Ras-synthetic lethality in mutant RAS oncogene-expressing cells (IC50s = 25 and 578 nM for HRASG12V-expressing and wild-type RAS-expressing BJeH cells, respectively)