Gel Loading Buffer II (Denaturing PAGE) from Thermo Fisher Scientific

Product Specs
ItemGel Loading Buffer II (Denaturing PAGE)
CompanyThermo Fisher Scientific
Price Inquire
Catalog NumberAM8547
FormatBottle, Liquid
Quantity10 mL
TypeSample Loading Buffers

Specifications/Features

Label or Dye: Bromophenol Blue

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Cellular assembly and functional resilience of the mammalian RNA exosomeThe EMBO JournalJune 25, 2026Tsimafei Navalayeu, Nikolaus Beer, Monika Bebjaková, Robert W Kalis, Ulrich Hohmann, Karel Stejskal, Gabriela Krššáková, Nina Fasching, Veronika A Herzog, Niko Popitsch, et al.Membrane, PVDF, 0.45 µm",EMD Millipore,Cat#IPVH00010 Gel Loading Buffer II,Thermo Fisher Scientific,Cat#AM8547 GlutaMAX Supplement,Gibco,Cat#35050061 L-Glutamine,Gibco,Cat#25030081 Lipofectamine 2000,Thermo Fisher Scientific

(2) OGG1 increases exercise endurance via elevated skeletal muscle FGF21Journal of Biological ChemistryMay 1, 2026Bhavya Blaze, Priyanka Sharma, Bhavya Prakash Gupta, Souvik Mandal, Sai Santosh Babu Santosh Babu Komakula, Tracy Anthony, Emmanuel Marfo, Harini Sampathdescribed previously (37). The reaction was stopped by adding an equal volume of gel loading buffer II (AM8547, Thermo Fisher) containing formamide and heated at 95 °C for 5 min. The excised substrate and product

(3) Ligase‐Catalyzed Transcription and Reverse Transcription of XNA by T3 DNA LigaseCurrent ProtocolsJanuary 12, 2026Natalie Khamissi, Luca Rustico, Ryan HiliD6492‐02)100% ethanol (required for Omega kit)2× gel loading buffer with dyes (Invitrogen, cat. no. AM8547)10% precast polyacrylamide TBE‐urea gel (Bio‐Rad, cat. no. 4566033)1× TBE buffer (see recipe)Low‐molecular‐weight

(4) The DinG exonuclease acts as a primary quality controller to remove unprocessed ribosomal RNAsNucleic Acids ResearchJanuary 6, 2026Karolis Vaitkevičius, Jörgen Johanssonlabeled oligonucleotide substrates were at defined time points mixed with a Gel Loading Buffer II (AM8547, Thermo Fisher Scientific) and frozen in liquid nitrogen for temporary storage. The samples were run

(5) Mechanistic study of glutamine metabolic reprogramming driving non-small cell lung cancer progression via the FGF17-FGFR4 axis mediating epithelial-mesenchymal transitionFrontiers in Molecular BiosciencesJanuary 2, 2026Qinghua Kong, Xiaoyan Wang, Wei Dingto measure protein levels. The samples were then denatured by being boiled in 5×SDS loading buffer (AM8547, Thermo) at 95 °C for 10 min. Separation of approximately 30 μg of total protein per lane was performed

(6) Transcriptomics and Proteomics Identify Serotonin Transporter as a Promising Therapeutic Target for Essential TremorMolecular & Cellular ProteomicsNovember 4, 2025Lingbing Wang, Zhuofan Zhou, Suzhen Lin, Yanjing Li, Shaoyi Zhang, Tian-Le Xu, Xing-Lei Song, Yiwen WuAfter sonication and centrifugation, the supernatant was combined with loading buffer (Thermo Fisher, AM8547). Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher, 23225).

(7) Functional microRNA targeting without seed pairingNucleic Acids ResOctober 16, 2025Matthew H Hall, Peter Y Wang, Thy M Pham, David P BartelSigma-Aldrich, P2069), ethanol precipitated, and re-suspended in 10 μL Gel Loading Buffer II (Invitrogen, AM8547). In parallel, some input RNA mixture was subjected to the same Proteinase K digestion, phenol-chloroform

(8) Repeated emergence of giant microRNA hairpins across invertebratesCell ReportsSeptember 23, 2025Mir-Mammad Javad-zada, Xiaodong Zou, Katsutomo Okamura, Renfu Shang, Ankur Garg, Ethan Lee, Leemor Joshua-Tor, Bastian Fromm, Eric C. LaiT4 Polynucleotide Kinase,NEB,Cat#M0201L T4 DNA Ligase,NEB,Cat#M0202L Gel Loading Buffer II,Invitrogen,Cat#AM8547 Strep-Tactin Superflow high-capacity resin,IBA Lifesciences,Cat# 2-1208-025 5-aminolevulinic acid hydrochloride

(9) Profiling RNA G‐Quadruplexes In vivoCurrent ProtocolsSeptember 19, 2025Bibo Yang, Yiliang Ding, Yueying Zhang(Sigma‐Aldrich, S6546)Circligase ssDNA Ligase (Epicentre, CL9021K)Gel loading buffer II (Invitrogen, AM8547)Novex TBE‐Urea Gels, 15% 10‐well (Invitrogen, EC6885BOX)SYBR Gold (Invitrogen, S11494)TEN250 buffer,

(10) AND-1 is a critical regulator of R-loop dynamics and a target to overcome endocrine resistanceSci AdvAugust 8, 2025Zhuqing Li, Yihan Peng, Huai-Chin Chiang, Yanjun Gao, Chen Chu, Yi Zhang, Muhammad Jameel Mughal, Chengyu Liu, Rong Li, Huadong Pei, et al.min at 37°C. Each sample was mixed with denaturing PAGE gel loading buffer (Thermo Fisher Scientific, AM8547) and was analyzed by 12% denaturing PAGE with 7 M urea in 0.5× TBE buffer. Gels were exposed at 600

(11) PAL-AI reveals genetic determinants that control poly(A)-tail length during oocyte maturation, with relevance to human fertilityNat CommunAugust 1, 2025Kehui Xiang, David P. BartelBefore gel loading, 2 µl of 0.5 M EDTA (pH 8.0) and 100 µl 2× Gel Loading Buffer II (Thermo Fisher, AM8547) were added. Samples were incubated at 65 °C for 5 min, then resolved on 5% urea–acrylamide denaturing

(12) Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjectionSTAR ProtocMay 9, 2025Grace S. Lee, Natalie A. Trigg, Colin C. Conine(NaOAc),Fisher Scientific,AM9740 2× RNA loading dye,NEB,B0363 Gel loading buffer II,Thermo Fisher Scientific,AM8547 Single-stranded RNA ladder,NEB,N0362 PMSG,Fisher Scientific,HOR-272-A hCG,Millipore,CG10-1VL KSOM,Millipore,MR-101-D Mineral oil

(13) DragonRNA: Generality of DNA-primed RNA-extension activities by DNA-directed RNA polymerasesNucleic Acids ResApril 8, 2025Emily Greenwald, Drew Galls, Joon Park, Nimit Jain, Stephen B Montgomery, Bijoyita Roy, Y Whitney Yin, Andrew Z Fire(SDS). Following quench, the crude products were mixed with 75% (v/v) Gel Loading Buffer II (Ambion AM8547), the mixture heated to 95°C for 5 min, and run on a pre-run 10% or 15% TBE-Urea gel (Novex 10% EC6875BOX,

(14) Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activityElifeMarch 13, 2025Shihui Chen, Carolyn Marie PhillipsDNA,ThermoFisher,Cat# SM1213, "Chemical compound, drug",Ambion RNA loading buffer II,ThermoFisher,Cat# AM8547, "Chemical compound, drug",SYBR Gold Nucleic Acid Gel Stain,Life Technologies,Cat# S11494, "Chemical

(15) Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activityElifeMarch 13, 2025Shihui Chen, Carolyn Marie PhillipsDNA,ThermoFisher,Cat# SM1213, "Chemical compound, drug",Ambion RNA loading buffer II,ThermoFisher,Cat# AM8547, "Chemical compound, drug",SYBR Gold Nucleic Acid Gel Stain,Life Technologies,Cat# S11494, "Chemical

(16) The structural basis for RNA slicing by human Argonaute2Cell ReportsJanuary 28, 2025Abdallah A. Mohamed, Peter Y. Wang, David P. Bartel, Seychelle M. VosDNase,Promega,Cat#M6101 SUPERase · In,Invitrogen,Cat#AM2694 Gel Loading Buffer II,Invitrogen,Cat#AM8547 cOmplete Mini EDTA-free Protease Inhibitor Cocktail,Roche,Cat#11836170001 cOmplete EDTA-free Protease

(17) CDH3-AS1 antisense RNA enhances P-cadherin translation and acts as a tumor suppressor in melanomabioRxivDecember 28, 2024Manon Chadourne, Crystal Griffith, Xiaonan Xu, Emily Brennan, Olga Vera, Nicol Mecozzi, Kaizhen Wang, Alex M. Jaeger, Florian A. Karreth12% TBE Urea precast gel (Thermo Fisher Scientific). 5 μL of Gel Loading Buffer (Thermo Fisher, Cat # AM8547) was added to the resuspended RNA and loaded into one or two wells of the gel alongside a 30-nucleotide

(18) Novel insights into the phenotypic spectrum and pathogenesis of Hardikar syndromeGenetics in MedicineOctober 1, 2024Alanna Strong, Michael E. March, Christopher J. Cardinale, Yichuan Liu, Mark R. Battig, Livia Sertori Sertori Finoti, Leticia S. Matsuoka, Deborah Watson, Sindura Sridhar, James F. Jarrett, et al.Dharmafect 4 per mL in Opti-MEM. Immunoblot: Cells were lysed in 1xLDS sample buffer (Invitrogen, AM8547). Samples were run on 4 – 12% NuPAGE MOPS/SDS gels (ThermoFisher Scientific) per the manufacturer’s

(19) The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complexMolecular CellAugust 8, 2024Peter Y. Wang, David P. Bartel0.025% [w./v.] xylene cyan, 0.025% [w./v.] bromophenol blue) or Gel Loading Buffer II (Invitrogen, AM8547) at 90°C for 1 min. The full-length RNA product was then purified on a 12% or 15% urea-polyacrylamide

(20) Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2iScienceJuly 19, 2024Planchais C, Fernández I, Chalopin B, Bruel T, Rosenbaum P, Beretta M, Dimitrov JD, Conquet L, Donati F, Prot M, et al.Biotech,N/A Polyethylenimine,Polysciences,#23966-2 Hoechst 33342,Invitrogen,#H3570 Denaturing Sample Loading Buffer,Invitrogen,#AM8547 Reducing Agent NuPAGE,Invitrogen,#NP0004 Paraformaldehyde 32% Aqueous Solution EM Grade,Electron Microscopy

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Gel Loading Buffer II (Denaturing PAGE) from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Gel Loading Buffer II (Denaturing PAGE) from Thermo Fisher Scientific

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