DAPI from Biotium, Inc.

Product Specs
ItemDAPI
CompanyBiotium, Inc.
Price $126.00Supplier Page View Company Product Page
Catalog Number40011
Quantity10MG
StorageStore with desiccant; Protect from light

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Biotium, Inc.

46117 Landing Parkway

Fremont, CA 94538

United States

Phone: 510-265-1027
510-265-1352

Email: order@biotium.com

Company Profile

Website: https://biotium.com/company/

(1) Genetic Identification of Dopamine Neurons Required for Circadian Food Anticipatory Activity in MicebioRxivMarch 31, 2026Andrew P. Villa, Jacqueline Trzeciak, Damien Wolfe, David E. Ehichioya, Jeffrey Falkenstein, Justine T. Wong, Lauren Dimalanta, Wyatt Kaiban, Jaskaran Dhanoa, Gregory Stevens, et al.Invitrogen, A11006), alongside DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:1000, Biotium, #40011) for nuclei staining. Sections were mounted using Immu-Mount (Epredia, #9990402) and imaging was performed

(2) Involvement of AKT/β-catenin in Enhanced Osteogenesis by Cannabidiol in Bone Stromal Cells Harvested from Human ToriScientific ReportsFebruary 20, 2026Anupong Makeudom, Mananchaya Taechukorn, Kanyanut Muenhong, Pantira Danvirunhavanit, Janthima Petchnuan, Ekapong Dechtham, Pansakorn Sangkaew, Sumit Suamphan, Suttichai Krisanaprakornkitanti-rabbit IgG antibody (1:2,000; #NL004; R&D Systems, Inc., Minneapolis, MN, USA) and 1 µM DAPI (#40011; Biotium, Inc., Hayward, CA, USA). Subsequently, the coverslips were mounted using fluorescence mounting

(3) Inhibition of Proteasome LMP2 Activity Suppresses Chil3 Expression in Mouse Colon Adenocarcinoma Tissue and Restrains Tumor GrowthOncology ResearchAugust 28, 2025Tatiana M. Astakhova, Nikita S. Karpov, Nataliya O. Dashenkova, Elena V. Alpeeva, Mikhail V. Nesterchuk, Sergey B. Akopov, Arsen S. Mikaelyan, Anfisa S. Ryabchenko, Pavel A. Erokhov, Natalia P. SharovaThermo Fisher Scientific) for 1.5 h in the absence of light, washed with PBS and incubated with DAPI (#40011, Biotium, Fremont, CA, USA) (5 μM) for 10 min. After fluorescence microscopy (Olympus IX73 microscope

(4) Rat Islet pECM Hydrogel-Based Microencapsulation: A Protective Niche for XenotransplantationGelsJuly 2, 2025Michal Skitel Moshe, Stasia Krishtul, Anastasia Brandis, Rotem Hayam, Shani Hamias, Mazal Faraj, Tzila Davidov, Inna Kovrigina, Limor Baruch, Marcelle Machluf(1:100, cell signaling, #5679), and MAFA (1:1000, cell signaling, #79737). DAPI (0.5 µg/mL, Biotium #40011) was used for DNA staining. Images were taken using LSM700 and LSM710 confocal microscopes (Carl Zeiss,

(5) Wheat Bran Polymer Scaffolds: Supporting Triple-Negative Breast Cancer Cell Growth and DevelopmentBioengineering (Basel)May 26, 2025Abulquasem Rayat Hossain, Md Sultan Mahmud, Kaydee Koistinen, George Davisson, Brooke Roeges, Hayle Boechler, Md Abdur Rahim Badsha, Md Rakib Hasan Khan, Michael Kjelland, Dorsa Fereydoonpour, et al.blocking for 4 h, and fluorescent stain 4′,6-Diamidino-2-Phenylindole or DAPI (~1 ug/mL), Cat. No. 40011 (Biotium, Inc., USA) for 30 min to stain cell nuclei for identification. Images were captured with a

(6) ECM Stiffness-Induced Redox Signaling Enhances Stearoyl Gemcitabine Efficacy in Pancreatic CancerCancers (Basel)March 3, 2025Shuqing Zhao, Edward Agyare, Xueyou Zhu, Jose Trevino, Sherise Rogers, Enrique Velazquez-Villarreal, Jason Brant, Payam Eliahoo, Jonathan Barajas, Ba Xuan Hoang, et al.cat. 65-111, Thermo Fisher Scientific) for 30 min and DAPI dihydrochloride (dilution = 1:100,000, cat. 40011, Biotium Inc., Fremont, CA, USA) (nuclei staining) in the dark for 5 min. After stopping the reaction

(7) A Comparison of in vivo Tumor-Homing Abilities of Placental-Derived and Bone Marrow-Derived Mesenchymal Stromal Cells in High-Risk NeuroblastomaJournal of pediatric surgeryJanuary 1, 2025Doyle K, Hassan AE, Sutter M, Rodriguez M, Kumar P, Brown E.antibody (A31573, Invitrogen ) for 1 hour at 25°C. Finally, the cell nuclei were counterstained with DAPI (40011; Biotium) for 5 minutes at 25°C. GFP analysis was performed by examining all sections using an inverted

(8) Melanoma progression and prognostic models drawn from single-cell, spatial maps of benign and malignant tumorsScience AdvancesJuly 12, 2024Nick R. Love, Claire Williams, Emily E. Killingbeck, Alexander Merleev, Mohammad Saffari Doost, Lan Yu, John D. McPherson, Hidetoshi Mori, Alexander D. Borowsky, Emanual Maverakis, et al.clone: 30-9, concentration of 1:100), anti–Melan-A (Dako, clone: A103, RTU), and DAPI (Biotium, no. 40011, 5 μg/mL as a final concentration). The BOND RX fully automated research stainer (Leica) was used for

(9) Neuronal DAMPs exacerbate neurodegeneration via astrocytic RIPK3 signalingJCI insightMay 7, 2024Chang NP, DaPrano EM, Lindman M, Estevez I, Chou TW, Evans WR, Nissenbaum M, McCourt M, Alzate D, Atkins C, et al.temperature. Slides were washed 3 times to remove secondary antibody and were then stained with DAPI (Biotium, 40011) diluted in PBS for 20 minutes at room temperature, followed by another wash. Sections were coverslipped

(10) Matrix-Assisted Cell Transplantation for the Treatment of Limbal Stem Cell Deficiency in a Rabbit ModelBiomedicinesJanuary 3, 2024Yu Y, Andreev AY, Rogovaya OS, Subbot AM, Domogatsky SP, Avetisov SE, Vorotelyak EA, Osidak EO.(donkey-anti-mouse, 1:1000, Invitrogen, Carlsbad CA, USA; A32773). The nuclei were stained with DAPI dye 40011 (Biotium, Fremont, CA, USA) at a dilution of 1:2000 for 10 min. Images were captured using an inverted

(11) Neuronal DAMPs exacerbate neurodegeneration via astrocytic RIPK3 signalingbioRxiv : the preprint server for biologyJuly 24, 2023Chang NP, DaPrano EM, Evans WR, Nissenbaum M, McCourt M, Alzate D, Lindman M, Chou TW, Atkins C, Kusnecov AW, et al.remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature, followed by another wash. Sections were cover-slipped

(12) Medullary tachykinin precursor 1 neurons promote rhythmic breathingeLifeJuly 17, 2023Jean-Philippe Rousseau, Andreea Furdui, Carolina da da Silveira Scarpellini, Richard L Horner, Gaspard MontandonCapture software,Corel,, "Software, algorithm",EthoVision XT,Noldus,,Version 14 Other,DAPI,Biotium,#40011,"4′,6-diamidino-2-phenylindole(fluorescent marker binding to DNA)" Other,Needle,P1 technologies,C315I-SPC

(13) Cultured meat platform developed through the structuring of edible microcarrier-derived microtissues with oleogel-based fat substituteNature communicationsMay 23, 2023Yen FC, Glusac J, Levi S, Zernov A, Baruch L, Davidovich-Pinhas M, Fishman A, Machluf M.cellularized microtissues with Phalloidin (actins, Sigma-Aldrich, P5282) and DAPI (nuclei, Biotium, 40011) using light-sheet fluorescence microscopy (Zeiss Z7, Germany). The generated 3D images were then used

(14) FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla CellsCellsSeptember 1, 2022Kashirina A, Gavrina A, Mozherov A, Kozlov D, Kuznetsova D, Vorotelyak E, Zagaynova E, Kalabusheva E, Kashina A.diluted 1:500 in a blocking solution overnight at 4 °C. The nuclei were contrasted with DAPI (cat #40011) (Biotium, Fremont, CA, USA). For imaging, cells were embedded in a supersaturated fructose solution. 2.5.

(15) Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1Molecular biology of the cellJune 1, 2022Powers RM, Daza R, Koehler AE, Courchet J, Calabrese B, Hevner RF, Halpain S.-568, and -647–conjugated secondary antibodies, 4′,6-diamidino-2-phenylindole (DAPI) (1:2000; Biotium 40011), Hoechst (1:1000; Pierce 33258), or phalloidin (1:250; Invitrogen) in blocking buffer at 37°C for 45

(16) A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancyNature CancerJanuary 1, 2022Julie S. Di Martino, Ana Rita Nobre, Chandrani Mondal, Isra Taha, Eduardo F. Farias, Elana J. Fertig, Alexandra Naba, Julio A. Aguirre-Ghiso, Jose Javier Bravo-Corderosecondary antibodies (AlexaFluor, Molecular Probes) for 2 hours at room temperature, and in DAPI (Biotium 40011) to label the DNA. Tissue was washed in PBS 1X three times with a final wash in distilled water. Coverslip

(17) Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosisCell death discoveryAugust 12, 2021Feng YH, Li LF, Zhang Q, Zhang JH, Huang Y, Lv YL, Jia JZ, Zhang D, Hu JY, Huang YS.the corresponding secondary antibodies for 1 h at 37 °C. Nuclei were stained for 5 min with DAPI (#40011, Biotium, Inc) or Hoechst 33342 (#H1399, Invitrogen) or PI (propidium iodide, #P1304MP, Invitrogen).

(18) A modified parachute assay for assessment of gap junction intercellular communication in placental trophoblast cellsToxicology mechanisms and methodsJune 1, 2021Gingrich J, Pu Y, Veiga-Lopez A.354217) CW008 (Tocris Bioscience, Bristol, UK; Cat. #: 5495) DAPI (Biotium, Hayward, CA, US; Cat. #: 40011) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, US; Cat. #: 472301) DMEM/F12 cell culture media

(19) Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activationChinese medicineSeptember 22, 2020Wu MH, Lin CY, Hou CY, Sheu MT, Chang H.and TLR4 detection, respectively. Hoechst 33324 (H3570; Invitrogen, Paisley, UK) and DAPI (Biotium 40011, Fremont, CA, USA) were used for nuclear staining. The samples were analyzed through fluorescence microscopy

(20) Real-time imaging of senescence in tumors with DNA damageScientific ReportsDecember 1, 2019Ying Wang, Jun Liu, Xiaowei Ma, Chao Cui, Philip R. Deenik, Paul K. P. Henderson, Ashton L. Sigler, Lina Cui(1:500) at room temperature for 1 hour, and counter-stained with diamidino-phenyl-indole (DAPI, Catalog #40011, Biotium, USA) for 5 min. After washing, all cover slides were mounted by ProLong Gold Antifade Mountant

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Biotium, Inc.

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DAPI from Biotium, Inc.

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